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Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276On-line version ISSN 1678-8060

Mem. Inst. Oswaldo Cruz vol.90 no.4 Rio de Janeiro July/Aug. 1995 

Antibody isotypes, including IgG subclasses, in Ecuadorian patients with pulmonary Paragonimiasis

Angel Guevara E.1 

Juan Carlos Vieira G.2 

Edmundo Araujo N.2 

Manuel Calvopiña H.2 

Ronald H. Guderian2 

Yves Carlier3 

Universite Libre de Bruxelles, Laboratoire de Parasilogie, Bruxelles, Belgium

Hospital Vozandes, Department of Clinital Investigations, Quito, Ecuador

Universitef Libre de Bruxelles, Laboratoire de Parasilogie, Bruxelles, Belgium


An ELISA test was developed to detect Paragonimus-specific antibodies, including IgG subclasses, using P. mexicanus crude water-soluble antigens. The test was standardized to detect antibodies in sera of Ecuadorian patients with pulmonary paragonimiasis and negative controls from the endemic area. The detected mean levels of IgG (0.753, SEM: 0.074) and IgM (0.303, SEM: 0.033) were significantly elevated (P<0.05). Within the IgG subclasses, IgG4 showed the highest detected mean level (0.365, SEM: 0.116) and the other three subclasses showed considerably lower mean levels (IgG1, 0.186 SEM: 0.06; IgG2, 0.046 SEM: 0.01; IgG3, 0.123 SEM: 0.047). The number of P. mexicanus eggs found in sputum of infected individuals showed a positive correlation with the level of antibodies detected for IgM, IgG and its subclasses (P<0.001). The relevance of these findings in Ecuadorian patients suffering from pulmonary paragonimiasis is discussed.

Key words: cuador; pulmonary paragonimiasis; IgM; IgG; IgG subclasses


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