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Revista Brasileira de Zoologia

Print version ISSN 0101-8175

Rev. Bras. Zool. vol.18 no.2 Curitiba June 2001

https://doi.org/10.1590/S0101-81752001000200018 

Polimorfismo enzimático em populações de Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera, Apidae, Meliponinae)

 

Enzymatic polymorphism in Melipona quadrifasciata anthidioides Lepeletier populations (Hymenoptera, Apidae, Meliponinae)

 

 

Davi S. Aidar; Rubiane C. Pagotto; Eucleia P.B. Contel

Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo. Avenida Bandeirantes 3900, Monte Alegre, 14049-900 Ribeirão Preto, São Paulo, Brasil

 

 


ABSTRACT

Them aim scope of this study is to characterize the enzymatic polymorphism found in the Melipona quadrifasciata Lepeletier, 1936 populations from Ribeirão Preto, São Paulo and Espírito Santo, Brazil and its hybrids. Samples from each colony (about 52) were prepared for starch gel electrophoresis in order to investigate the genetic variation of the following enzimes: esterase (EST), isocitrate dehydrogenase (IDH), malic enzyme (ME), phosphoglucomutase (PGM), superoxide desmutase (SOD), α-glycerophosphate dehydrogenase (αPGD), malate dehydrogenase (MDH), leucine aminopeptidase (LAP), hexokinase (HK) and phosphoglucose isomerase (PGI). The analysis showed that LAP and HK did not show enzymatic activity and EST showed two alleles(est-sand and est-f) while all the others were shown to be monomorphic. The allele EST-S showed a frequency of 82,6%.

Key words: Melipona quadrifasciata anthidioides, polymorphism, electrophoresis, enzyme, stingless bees


 

 

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Full text available only in PDF format.

 

 

AGRADECIMENTOS. Agradecemos ao CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) e FAPEMIG (Fundação de Amparo a Pesquisas de Minas Gerais) pelos recursos doados. Ao Prof. Warwick Estevam Kerr, agradecemos pela leitura do manuscrito e pelas sugestões e pela orientação.

 

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Recebido em 29.VI.2000; aceito em 22.V.2001.

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