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Acta Botanica Brasilica

Print version ISSN 0102-3306

Acta Bot. Bras. vol.6 no.2 Feira de Santana Dec. 1992

https://doi.org/10.1590/S0102-33061992000200006 

Regeneração de plantas a partir de panículas imaturas de arroz (Oryza sativa L.)*

 

Regeneration of rice plants (Oryza sativa L.) from immature panicles

 

 

Liane T. DornellesI; José Antônio PetersII

IBolsista CAPES - UFPel
IIUFPel/Departamento de Botânica, Campus Universitário, C.P. 345, CEP 96100 - Pelotas - RS

 

 


RESUMO

Com o objetivo de verificar o potencial de regeneração direta e indireta, panículas imaturas de duas cultivares de arroz (BR IRGA 410 e 414) foram cultivadas em meio mineral de Murashige & Skoog, contendo lmg/1 e 2mg/l de 2,4-D ou 2mg/l de 2,4-D mais 0,2mg/l de K para indução de calos e lmg/l de ANA e 4 mg/l de K para regeneração direta de gemas. Em ambos os genótipos 100% das panículas formaram calos, porém sua regeneração foi dependente do meio de formação dos calos. Na regeneração direta 100% das panículas nas duas cultivares formaram gemas, com médias de 5,8 gemas/panículas.
ABREVIATURAS: MS - meio básico de Murashige & Skoog; 2,4-D - ácido 2,4 diclorofenoxiacético; K - cinetina; AIA - ácido 3-indolacético; ANA - ácido naftalenoacético;

Palavras-chave: Oryza sativa L.; regeneração; panículas imaturas


ABSTRACT

Aiming to observe the direct and indirect regeneration potencial, immature panicles of rice cultivars BR IRGA 410 and 414 were cultured on Murashige & Skoog's basal medium complemented with either lmg/l or 2 mg/l 2,4-D or 2 mg/12,4-D or 2 mg/l 2,4-D + 0,2 mg/l K for callus induction and with 1 mg/l of ANA + 4 mg/l of K for direct bud regeneration. In both genotypes, 100% of the panicles developed callus, but the regeneration showed dependency on the callus forming medium. Throguh direct regeneration, 100% of the panicles from both cultivars developed buds with average of 5.8 buds/panicle.
ABREVIATION: Murashige, and Skoog medium; 2,4-D - ácido 2,4 doclorofenoxiacetic acid; K - Kinetin; AIA - indole - 3 - acetic acid; ANA - acido - naphthaleneacetic acid.

Key words: Oryza sativa L.; regeneration; immature panicles.


 

 

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

 

 

Referências Bibliográficas

BAJAJ, Y.P.S. & M. BIDANI. 1980. Differentiation of genetically variable plants from embryo-derived callus cultures of rice. Phytomorphology 30(213): 290-4        [ Links ]

BRAR, D.S.; D.H. LING S. YOSHIDA. 1985. Plant regeneration from somatic cell cultures of some IR varieties of rice. In: Biotechology in International Agricultural Research, Manila, International Rice Research Institute, p. 169-77.         [ Links ]

CHOU, K. T.; K.L.GE; I.S. TSAI; C.S. YANG & H.W. YANG 1983. Callus induction and redifferentiation of different hybrid rice plant parts. In: Cell and Tissue Culture Techniques for cereal Crop Improvement Science Press, Beijing, p. 207-13.         [ Links ]

CHU, C. C.; C.C. WANG; C.S. SUN; C. HSU; K.C. YIN & C.Y.CHU 1975. Establishment of an efficient medium for anther nitrogen sources. Scientia Sinica 18:659-68.         [ Links ]

KOTT, L.S.; M.J. HOWART; R.L. PETERSON & K.J. KASHA 1985. Light and electron microscopy of callus initiaton from haploid barley embryos. Can. J. Bot 63:1801-5.         [ Links ]

LING, D.H.; W.Y. CHEN M.F. CHEN; Z.R. MA 1983. Direct development of plantlets from immature panicles of rice in vitro. Pant Cell Reports 2:172-4.         [ Links ]

MIKAMI, T. & T. KINOSHITA 1988. Genotypic effects on the callus formation from differnt explants of rice, Oryza sativa L. Plant Cell, Tissue and Organ Culture 12: 311-4         [ Links ]

MURASHIGE, T. & F. SKOOG. 1962. A revised for rapid growth and bioassays with tabacco tissue cultures. Physiol. Plant 15: 473-97.         [ Links ]

RAINA, S.K. 1989. Tissue culture in rice improvement: status and potencial. Advances in Agronomy 42: 339-98.         [ Links ]

 

 

Recebido em 16-09-91.
Aceito em 18-02-93.

 

 

* Parte da Dissertação de Mestrado em agronomia do primeiro autor, Faculdade de Agronomia da UFPEL. Trabalho apresentado no XLII Congresso Nacional de Botânica, Goiânia, GO.

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