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Ciência Rural

Print version ISSN 0103-8478On-line version ISSN 1678-4596

Cienc. Rural vol.48 no.7 Santa Maria  2018  Epub July 02, 2018

https://doi.org/10.1590/0103-8478cr20170206 

CLINIC AND SURGERY

Hyaluronic acid and synovial fluid in preventing adhesion formation after tenorrhaphy: An in vivo study on rabbit Achilles tendon

Ácido hialurônico e líquido sinovial na prevenção da formação de aderências após tenorrafia: Um estudo in vivo sobre o tendão de Aquiles de coelho

1Department of Surgery, Faculty of Veterinary Medicine, Kafkas University, TR36100, Kars, Turkey.

2 Department of Pathology, Faculty of Veterinary Medicine, Ondokuz Mayıs University, Samsun, Turkey.

3 Department of Orthopaedics and Traumatology, Faculty of Medicine, Kafkas University, Kars, Turkey.

4 Department of Mechanical Engineering, Faculty of Engineering and Architecture, Kafkas University, Kars, Turkey.


ABSTRACT:

Peritendinous adhesions are a significant problem in tendon surgery. One of the most preferred methods for preventing adhesion formation is anti-adhesive applications. This study aimed to investigate the efficacy of natural synovial fluid as an alternative viscoelastic agent for preventing adhesion in the healing period compared to hyaluronic acid. Thirty adult New Zealand rabbits were used in the study to form three experimental models. In all groups, a rupture was induced in the Achilles tendons. Following tenorrhaphy, hyaluronic acid was applied in one group and synovial fluid in the other group for anti-adhesive purposes. In the control group, no viscoelastic application was made. Bandage was applied to the operated extremities for three weeks post-operatively. At the end of five weeks, euthanasia was performed on the animals using pentobarbital sodium. Surgical sites in all groups were macroscopically examined for healing status as well as the presence and extent of adhesions. Biomechanical tests and histopathological examinations were then performed on the tendons in all groups. Findings established once again the positive contribution of hyaluronic acid to preventing adhesion formation as well as to healing and tensile strength in tendon surgery. Although, we found that it is possible to use synovial fluid, which is a natural source of hyaluronic acid, as a viscoelastic material, it is not superior to commercial hyaluronic acid preparation.

Key words: Achilles tendon; adhesion; synovial fluid; hyaluronic acid; rabbit

RESUMO:

Asderências peritendinares são um problema significativo na cirurgia tendínea. Um dos métodos mais empregados para evitar a formação de adesão são as aplicações anti-adesivas. Este estudo tem como objetivo investigar a eficácia do líquido sinovial natural como um agente viscoelástico alternativo para prevenir a adesão no período de cicatrização comparado ao ácido hialurônico. Trinta coelhos adultos da Nova Zelândia foram utilizados no estudo para formar três modelos experimentais. Em todos os grupos, uma ruptura foi induzida nos tendões de Aquiles. Após tenorrafia, o ácido hialurônico foi aplicado em um grupo e o líquido sinovial no outro grupo para fins anti-adesivos. No grupo controle, não foi feita qualquer aplicação viscoelástica. A bandagem foi aplicada às extremidades operadas por três semanas pós-operatório. No final de cinco semanas, a eutanásia foi realizada nos animais utilizando pentobarbital sódico. Os locais cirúrgicos em todos os grupos foram examinados macroscopicamente quanto ao estado de cura, bem como a presença e extensão das aderências. Testes biomecânicos e exames histopatológicos foram realizados nos tendões em todos os grupos. Os achados estabeleceram mais uma vez a contribuição positiva do ácido hialurônico para prevenir a formação de aderências, bem como para a cicatrização e resistência à tração na cirurgia do tendão. Embora se tenha constatado que é possível utilizar fluido sinovial, que é uma fonte natural de ácido hialurónico, como material viscoelástico, não é superior à preparação de ácido hialurónico comercial.

Palavras-chave: tendão de Aquiles; aderência; fluido sinovial; acido hialurônico; técnica de sutura Kessler modificada; coelho

INTRODUCTION:

Tendon injuries are one of the most common orthopaedic problems. They are usually accompanied by trauma to bones and the surrounding soft tissues (SLATTER, 1985; AYGÜN, 2013). Blood vessels, tenocytes, and collagen fibres that regenerate after a trauma in addition to suture procedures and immobility facilitate adhesion formation at the site (KHANNA et al., 2009; ORYAN et al., 2011; ÖZGENEL et al., 2001). The most significant factor of interest to clinicians in tendon healing is tendon adhesion to surrounding tissues, which causes a decrease in the gliding function of the tendon (LUI, 2007; ÖZGENEL et al., 2001; SLATTER, 1985).

No matter which technique is selected, the objectives are the same in all tendon surgeries. These are maintaining physical integrity of the original structure, returning tendon strength to the pre-injury condition, and restore the range of motion to pre-injury standards by minimising adhesion to the surrounding tissues (LIU et al., 2008; ÖZGENEL et al., 2001; SLATTER, 1985). Various physical barriers and biological membranes have been developed to reduce or prevent adhesions. Some of these methods include polyethylene, silicone, or chondroitin sulphate membrane (LIU et al., 2008) application and placement of an arterial sheath graft on the tendon (ÖZAYDIN et al., 1995).

Studies established the need to apply viscoelastic agents that do not affect the healing process negatively and at the same time can also protect the gliding function of the tendon for successful tendon treatment. Hyaluronic acid has been used in joint, bone, eye and ear surgery for its anti-inflammatory and anti-adhesive properties for a long time (ORYAN et al., 2011). It is also one of the most commonly used viscoelastic agents for limiting post-operative tendon adhesion (LIU et al., 2008; ÖZGENEL et al., 2001). Synovial fluid contains a high amount of hyaluronic acid (3-4mg/mL) (HUI et al., 2012). It has been used as a viscosupplementation and anti-inflammatory agent in joint diseases (LIPOWITZ, 1985; ÖZAYDIN & KOÇ, 1993).

The main idea of this study was to find out whether or not synovial fluid is effective in preventing tendon adhesion due to its high hyaluronic acid content and viscoelastic properties. For this purpose, we planned to investigate the efficacy of synovial fluid compared to commercial hyaluronic acid in a rabbit Achilles tendon model for reducing adhesion, which is one of the most common complications occurring after tendon operations in human medicine and veterinary practice.

MATERIALS AND METHODS:

Animal material and experimental groups

The animal material for the study consisted of 30 adult female (non-pregnant) white New Zealand rabbits weighing 3.5-4.0 kg. The study was conducted in three groups, each of which consisted of 10 rabbits: Hyaluronic Acid (HA), Synovial Fluid (SF), and Control (C) groups.

Study plan

In all groups, the operations were performed on right hindlimb and under dissociative anaesthesia (5mg/kg, Xylazine HCl, Rompun®, BAYER-50mg/kg, Ketamine HCl, Ketasol®, RICHTER PHARMA AG). A complete incision of the Achilles tendon was performed and repaired with the Modified Kessler technique using 4/0 polypropylene (Prolene®, ETHICON) suture material. An anti-adhesive gel (0.5ml) containing hyaluronic acid (10mg/ml, Hyalgan®, FIDIA) was applied to the suture line in the HA group and synovial fluid (1.5ml) in the SF group. The amount of synovial fluid (1.5ml) used in the present study was adjusted to the commercial anti-adhesive gel which contains 10mg/ml hyaluronic acid as synovial fluid contains approximately 3mg/ml hyaluronic acid. In group C, no anti-adhesive was used after tenorrhaphy. The site was closed using the routine technique.

In the present study synovial fluid collected from bovine was used as it was not possible to collect required amount of synovial fluid from joints of rabbits. Synovial fluid specimens collected from the tarsal joints of healthy cattle were centrifuged to separate blood cells at 3000-3500 rpm for 10 minutes before application to the rabbit tendons to prevent inter-species antigen reaction.

Postoperative care and clinical observation

In all experimental groups, Cephazolin sodium (30mg/kg, IM, Cefamezin®, ZENTIVA) and Ibuprofen (10mg/kg, IV, Intrafen®, GEN) was administered postoperatively. The operated extremity was placed in a fiberglass orthopaedic cast tape (OrthoTape®, BEKSEL) and the bandages were kept clean and dry for three weeks. Animals were allowed to move freely in their individual cages. During this period, gait and function of the extremities of the animals was observed and clinically evaluated.

Ultrasonography

At the end of five weeks, ultrasonographic examination of the tendons was performed. A Pie Medical brand (Model: 100 Falco Vet) ultrasonography device and 8MHz linear probe was used. Transverse and longitudinal sections of the Achilles tendon were scanned, and the images were examined for the presence of tendon echogenicity and peritendinous adhesions.

uthanasia and evaluation of the results

Following ultrasonographic examinations, euthanasia was performed on the animals through intravenous (IV) administration of high-dose pentobarbital sodium (100mg/kg). In all experimental groups, the surgical sites were macroscopically examined for adhesion grading based on the length, density and, range of motion (Grade 1: No adhesion, Grade 2: Adhesion easily dissectible from the surrounding tissues, Grade 3: Adhesion not easily dissectible from the surrounding tissues, Grade 4: Adhesion involving 35-60% of the affected site, Grade 5: Adhesion involving more than 60% of the affected site) (LIU et al., 2008).

Each main group (HA, SF, and C) was then divided into two sub-groups: the biomechanical (B) test group (n=4) and the histopathological (H) examination (n=6) group. Tendons were totally excised and were subjected to tensile testing in group B. A Besmak brand Mechanical Tensile Testing Device was used for the procedure. In the tests performed at room temperature, tendons were placed between two jaws, immobile at the upper part and mobile at the lower part, and were strained at a rate of 10mm/min (till tendon rupture). Each test was repeated 3 times. The statistical average of the maximum load obtained from these three tests was used for evaluation (Table 1). The differences in tendon healing between the groups were determined based on the rupture point of tendons.

Table 1 Maximum load and average values obtained from the tensile tests on tendons. 

Groups Hyaluronic acid Control Synovial fluid
1 147.8N 141.4N 121.6N
2 183.4N 136.5N 112.5N
3 160.1N 151.1N 172.5N
Avg. 163.8N 143.0N 135.5N

In group H, tendon tissue specimens were fixed in a 10% formaldehyde solution. The tendon tissues were then soaked in 10% formic acid solution for three days for easy excision and rinsed under running tap water for 12 hours. Rinsed tendon tissue specimens were dehydrated in a tissue processing device (Leica, TP1020) with alcohol and xylene series and embedded longitudinally in paraffin. After being cut at a thickness of 5 microns using a microtome (Leica, RM2125RT), tendons were stained with Haematoxylin Eosin (HE) and Masson’s Trichrome histochemical staining methods. Stained tendon specimens were examined under a light microscope with a camera (Nikon, Eclipse E600, Nikon Inc., Melville, NY). The sections stained with Masson’s Trichrome and HE histochemical staining methods were evaluated for grade and severity of adhesion and quality of tendon healing by modifying the criteria reported by TANG et al. (1996) (Figure 1).

Figure 1 Average values of the semi-quantitative analysis results of tendon adhesion and tendon healing in the control and experimental groups. 

RESULTS:

Clinical observation findings

From the first day after the operation, animals in all groups could stand on their bandaged extremities, and walk even though they avoided the affected extremity. Animals in the HA and SF experimental groups were found to be more attentive to their surroundings and more active. At the end of three weeks, bandages were removed and animals were allowed to walk freely for adaptation. Slight limping was observed for three days in all groups. Following 24 days of healing period after the operation, all rabbits could walk without any difficulty; they could even perform activities such as jumping, running, etc. in their cages. However, asymmetry has been observed between the healthy and operated limbs in two animals from each group.

Ultrasonographic examination findings

In all experimental group, the tendons had increased in thickness and echogenicity, and heterogenous echotexture (C>SF>HA). In addition, due to irregular echogenicity along the tendon, interruption of the fibrillar echotexture, and the presence of hypoechoic intratendinous foci in two of these rabbits in the HA group, one in the SF group, and one in the C group, the tendons were considered suspect of rupture.

Macroscopic examination findings

The macroscopic examination revealed peritendinous adhesions with a focus on the suture line in all groups. In the HA experimental group, the tendon surfaces were smooth, and there were grade 2 adhesions in the form of fibrillary bands surrounding the suture line that could be easily dissected. In the SF experimental group, the tendon surfaces were partially smooth, and there were grade 3 adhesions between the suture line and peritendinous tissues in the form of thick bands that could not be easily dissected. In the control group, the tendons were thickened, the surfaces appeared rough, and there were grade 4 adhesions along the tendon extending to the periosteum in the tendinous and peritendinous line, with a focus on the suture line (Figure 2). A comparison of the tendons in terms of healing status revealed that two animals in each group developed tendon rupture, and the remaining ones healed without any problems.

Figure 2 Macroscopic appearances of the tendons after euthanasia. A: Group HA, grade 2 adhesion B: Group SF, grade 3 adhesion C: Group C, grade 4 adhesion.  

iomechanical test findings

In the HA group, the tendons ceased elongating and ruptured when they reached the maximum load. After rupturing, elongation continued incrementally for the rest of load application. In the SF and C group, no sudden change was observed in the load after reaching the maximum load. The average unit elongation values in all groups varied from 19 to 20mm. The data obtained during the tensile test were recorded by the computer and are reported in table 1.

Microscopic findings

According to the semi-quantitative analysis results, tendon adhesion in the HA group was more limited compared to the SF and C groups. Furthermore, the grade of tendon healing in the HA group was higher than the SF and C groups (Figures 3A, 3C). The grade of tendon healing was lower and the grade of tendon adhesion was higher in the SF group than in the HA group but better than group C (Figures 3A, 3B).

Figure 3 Microscopic appearances of tendons. A: Marked thickening in the paratenon due to vascularization (black arrows), fibrosis (green arrow), and inflammatory cell infiltration (red arrows). Peritendinous adhesions (brown arrows). Fibrils in the tendon that are not yet mature but are partially organized and has begun to appear more regular (yellow arrows). Group C, Masson’s Trichrome, x10 B: Paratenon with significant thickening due to fibrosis and vascularization (black arrows). Gap area (between green arrows) without adhesion between paratenon and tendon tissue that is not yet mature but is partially organized, and point where adhesion has begun (red arrow) between paratenon and tendon tissue. Group SF, Masson’s Trichrome, x2 C: Gap area where no adhesion has formed (between green arrows) between partially thickened paratenon (between black arrows) and tendon tissue that is highly organized. Group HA, Masson’s Trichrome, x4. 

In all experimental groups, there were significant adhesions characterized by loose fibrous filaments between the tendon and the surrounding epitenon and paratenon tissues, which thickened due to highly vascular and oedematous granulation tissue. Fibroblasts in this region were not yet organized and matured (Figure 3A). In the tendon tissue, the collagen was not yet mature but intratendinous collagen fibrils were partially organized and began to appear more regular (Figures 3A, 3B, 3C). In rare cases, the fibroblast nuclei between the collagen fibres at these sites began thinning and were located parallel to the collagen fibres (Figure 3C).

DISCUSSION AND CONCLUSION:

Cell proliferation as part of the healing physiology after tendon injuries, suture applications, and immobility facilitate adhesion formation. Although, alternative tenorrhaphy techniques have been developed, adhesions that form during the healing process and loss of function induced by this condition are still a significant problem in tendon surgery. This study investigated the efficacy of synovial fluid compared to a commercial hyaluronic acid preparation in preventing tendinous and peritendinous adhesions.

Some studies attempted to prevent adhesion using natural or synthetic barriers or viscoelastic materials (HUI et al., 2012; LIU et al., 2008; TAN et al., 2010). However, our literature review did not reveal any studies that used synovial fluid as an agent for preventing tendon adhesion. For this purpose, we applied a gel containing hyaluronic acid in one group, and synovial fluid in the other group after tenorrhaphy and then evaluated the tendons for adhesion formation.

When the general mobility of the animals was examined, clinical findings revealed that anti adhesive agent applied HA and SF groups performed better than that of the control group. This mobility increase, as ORYAN et al. (2011) indicated, is associated with pain control and the anti-degenerative properties of hyaluronic acid. Earlier tendon healing and the efficacy of the gliding function explain the increased activity of the animals in their cages. In human medicine, it is possible to weaken adhesions during the healing process through controlled minimal movements. However, because controlled voluntary movement is not possible in animals, this application may cause re-formation of tendon rupture and poor healing. SEVERO et al. (2012) argued that it is important to attempt to activate the site by week 3, when tendon healing takes place that mobility in the early period will not completely prevent adhesions but will help to re-gain normal function by ensuring tendon gliding. Conversely, some researchers suggested that three weeks is not adequate for tendon healing, and the extremity should remain in bandages for up to five weeks. In our study, we limited the duration of bandage application on the extremities to three weeks in order to eliminate the preliminary factors that impact adhesion formation in the postoperative period. At the end of three days after the bandages were removed, all the rabbits could walk without limping. Clinical findings showed that tendons re-gain their normal function after tenorrhaphy. However, tendon rupture developed in two animals in each experimental group. Results revealed the importance of extending the period of bandaging and providing minimal movements in tendon surgery. This condition demonstrated the dilemma of veterinarians when it comes to ensuring healing versus preventing adhesion formation in tendon surgery in the field of veterinary medicine.

Ultrasonographic diagnosis of inflammatory activity in tendons with paratenon can be made based on pathological images (MARTINOLI et al., 2002; PAAVOLA et al., 1998). In a study with horses, MARR et al. (1993) reported that the presence of fibrous adhesions can be diagnosed with ultrasonographic examination of peritendinous tissues, but warned that this evaluation may be subjective. In our study, in cases with suspected tendon rupture where ultrasonographic examination revealed severe adhesion, findings were confirmed with the macroscopic tendon examination after euthanasia. Although, the ruptured tendons of all animals had irregular echogenicity, intratendinous hypoechoic foci and interruption of the fibrillar echotexture, in one case from SF and in other case from C group could not be diagnosed ultrasonographically even though animals had ruptured tendons. Macroscopic tendon examination showed that there were partial ruptures in those tendons. Similarly, KAYSER et al. (2005) reported that ultrasonography is not sufficiently reliable for all pathologies of the tendons, especially partial ruptures of the Achilles tendon.

In macroscopic tendon examination, limited adhesion in the SF group compared to the C group demonstrates the anti-adhesive effect of the synovial fluid. However, results in the SF group fell short of those achieved in the HA group. Hyaluronic acid is known to remain at the injected site for approximately 34 days. Commercial HA preparation in gel formation creates a barrier between the tendon and the surrounding tissues and maintains its efficacy because it stays intact at the site for a long period of time. However, there is no information regarding how long synovial fluid stays in the region where it is applied. These findings obtained from our study support findings from the previous studies where hyaluronic acid is used as an anti-adhesive agent (HAGBERG&GERDIN, 1992; LIU et al., 2008; MORO-OKA et al., 2000; ORYAN et al., 2011).

In our study, when the tensile strengths of the tendons were compared among all groups, HA was superior to the other groups. When load values obtained from the tests were reviewed, strength differences were considered acceptable by previous studies as well (AYGÜN et al., 2010). The fact that elongation continued incrementally for the rest of the load application after rupture, particularly in the experimental group where HA was applied, was attributed to the flexible structure of the paratendinous tissue. AYGÜN et al. (2010) also reported that elongation continued during tensile tests even after rupture occurred due to the heterogenous structure of the tendons.

According to the histopathological examination, tendon adhesion was more limited in the SF group than in the C group. The fact that known effects of commercial HA preparations also occurred in the group where synovial fluid was applied suggests that it holds promise as an alternative. These findings, which are parallel to those reported by studies on anti-adhesive use in tendon surgery (HAGBERG&GERDIN, 1992; HUI et al, 2012; LIU et al., 2008; ORYAN et al., 2011), have once again established that application of viscoelastic materials, which are designed to maintain the gliding motion of the tendon for successful tendon healing during the operation is beneficial.

In conclusion, we demonstrated in this study that in addition to the use of HA as a commercial preparation to reduce or prevent adhesion formation, one of the most common complications occurring after tendon operations both in human and veterinary medicine, natural synovial fluid, which is a significant source of HA naturally found in the body, can also be used as a viscoelastic material. However, in order to better understand the role of synovial fluid in tendon healing, more extensive studies should be conducted. We believe that synovial fluid will be a good alternative to other agents as an affordable and effective treatment option in tendon surgery.

ACKNOWLEDGEMENTS

This study was supported by the Kafkas University Office of the Scientific Projects Coordinator. (Project No: KAÜ- BAP-2015-TS-65).

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0CR-2017-0206.R2

BIOETHICS AND BIOSSECURITY COMITTEE APPROVAL

1The study was conducted after approval was obtained from the Kafkas University Animal Experiments Local Ethics Committee (Approval No: KAÜ-HADYEK/2015-049). All applicable national guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of Animal Experiments Local Ethics Committee.

Received: March 25, 2017; Accepted: April 23, 2018; Revised: August 06, 2018

E-mail: basakyakin@hotmail.com. *Corresponding author.

The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.

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