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Brazilian Archives of Biology and Technology

Print version ISSN 1516-8913On-line version ISSN 1678-4324

Braz. arch. biol. technol. vol.51 no.spe Curitiba Dec. 2008

https://doi.org/10.1590/S1516-89132008000700007 

NUCLEAR MEDICINE: ONCOLOGY (DIAGNOSIS AND THERAPY)

 

Problems and pitfalls in vulvar and cervical cancer sentinel node scintigraphy

 

 

Helmut SinzingerI,II,*; Susanne GraneggerII; Barbara PalumboIII; Renato PalumboIII

IISOTOPIX -Institute for Nuclear Medicine; Mariannengasse 30; 1090; Vienna -Austria.
IIDepartment of Nuclear Medicine; Medical University of Vienna; Währinger Gürtel 18-20; A-1090; helmut.sinzinger@chello.at; Vienna - Austria.
IIINuclear Medicine Section; Department of Surgical, Radiological and Odontostomatological Sciences; Policlinico Monteluce; Perugia - Italy

 

 


ABSTRACT

After the introduction for penile cancer, the sentinel lymph node imaging is increasingly applied in various types of cancer. After the initial learning phase, 105 patients with vulvar and 24 with cervical cancer have been investigated. In vulvar cancer all the imaged sentinel nodes were discovered by the portable probe intraoperatively. No false negative sentinel node was observed. The most critical issue is the tracer application. Performed strictly intradermally, the sentinel node shows up immediately. Concomitant use of isosulfan blue dye did not improve the results and was stopped therefore. Similarly, more superficial (intra/subendothelial) application brings up better results as compared to deeper injection in cervical cancer patients. No false negative results were seen. Apparently, an almost 100% detection is possible. Our findings clearly show that tracer application is the key for successful imaging. If not done properly, sentinel node may appear later or may even more likely be missed.

Key words: sentinel lymph node -vulvar cancer -cervical cancer -scintigraphy


RESUMO

Após a introdução para câncer do pênis, a imagem do linfonodo sentinela é cada vez mais aplicada nos diversos tipos de câncer. Após a fase inicial de aprendizagem, 105 pacientes com câncer vulvar e 24 com câncer cervical foram investigados. No câncer vulvar todas as imagens de nodos sentinela foram descobertas por sonda portátil durante o exame. Nenhum nodo sentinela falso negativo foi observado. A questão mais crítica é a aplicação do traçador. Realizada pela via intradérmica, o nodo sentinela surge imediatamente. O corante isosulfan blue não melhora os resultados e seu uso concomitante foi abandonado. Do mesmo modo, a aplicação mais superficial (intra/subendotelial) apresenta melhores resultados quando comparada com a administração mais profunda em pacientes com câncer cervical. Não foram observados resultados falsos negativos. Aparentemente, uma detecção de aproximadamente 100% é possível. Nossos achados mostram claramente que a administração do traçador é um ponto chave para uma imagem com qualidade. Se não for feita corretamente, o nodo sentinela pode aparecer tardiamente ou pode até ser perdido.

Palavras-chave: linfonodo sentinela, câncer vulvar, câncer cervical, cintilografia.


 

 

INTRODUCTION

Cabanas in 1977 originally was defining sentinel lymph node in penile cancer patients. Already at this time lymph drainage imaging in melanoma has been done in many centers including ours to define the drainage of lymph flow without recognizing its unique clinical relevance. The unique concept says that a histologically negative sentinel lymph node predicts that the remaining lymph nodes will be tumor free. The reliability of this concept has been assessed in melanoma (Morton et al., 1992) and breast cancer patients (Cox et al., 1988) so far and is currently applied for a variety of other tumors such as in the maxillofacial region (Hyde et al., 2002), colorectal (Basilio et al., 2006), breast (Frisell et al., 2001), esophageal and gastric (Kitagawa et al., 2005), cervical (Hubalewska et al., 2003), vulvar (De Hullu et al., 2000) and penile (Valdés Olmos et al., 2001) cancer. We are reporting our experience with vulvar and cervical cancer. We performed the first vulvar cancer sentinel node scintigraphy in 1999 (Granegger et al., 1999) and cervical in 2001 (Sinzinger and Meghdadi, 2001), preliminary data were published in 2002.

 

PATIENTS AND METHODS

Patients (T1, T2) received a total of 12 MBq for a variety of other tumors such as in the 99mTc-nanocolloid (GE Healthcare Buchlar GmbH maxillofacial region (Hyde et al., 2002), colorectal and Co.KG, Braunschweig, Germany) in 1 ml (Basilio et al., 2006), breast (Frisell et al., 2001), saline intradermally divided in 4 parts in each quadrant around the tumor using an insulin syringe (BD 1 ml syringe, Luer-LokTM Tip; BD, New Jersey, USA). In cervical cancer 0.5 - 1.5 ml were used. Lymphoscintigraphy was performed with a double-headed gamma camera using a low energy high resolution collimator. Static images were performed at 5, 30, 60 and 90 minutes after tracer application. If possible, the sentinel was marked on the skin and the gamma camera images were available to the surgeon before starting the operation.

 

Table 1

 

Histopathological examination of the sentinel node and the other nodes was done after hematoxylin/eosine staining and serial sectioning. Nodes initially were cut into 2 to 3 mm slices and then each block again at 400 µdistance. The count rate in-vivo and in-vitro was measured, localization and count rate of all suspected sentinel nodes was documented. Radioactive nodes were separated and lymph node dissection performed. In negative sentinel nodes additional immunohistochemical examination was done.

 

RESULTS

Sentinel nodes appear mainly on the ipsi-lateral (tumor) side. From the ones showing up immediately presenting 1 sentinel only (n = 24, 22.86%), the overwhelming majority (n = 22, 22.95%) appeared on the ipsilateral side (Table 2). Only in 4 cases there were more than 2 sentinels showing up immediately on the ipsilateral side, no one at the contralateral side. In 5 patients (4.76%) a sentinel node was found up to 30 minutes, in 1 (0.95%) at 60 minutes. A total of 22 sentinel nodes (20.95%) were showing up as late as after 30 minutes, 7 others (6.67%) even later at 60 minutes. 22 patients (20.95%) showed 1 sentinel node immediately on both sides. In the other patients a variety of combinations was seen concerning site, number and time of appearance. In 6 patients a clear transport along the lymph vessel was discovered. The maximal number of sentinels seen in one patient was 5; 3 patients also presenting the primary tumor more than 1 cm from the midline, exhibited a sentinel node on the contralateral side, one of them showing even 2 nodes.

 

 

 

 

 

 

 

 

Cervical cancer sentinel nodes present mainly unilateral appearance sentinel localization next to bilateral along the pelvic wall and next to the the cervix is predominant. cervix (table 3), while in those cases withunilateral appearance sentinel localization next to the cervix is predominant.

 

DISCUSSION

Sentinel lymph node scintigraphy is now widely performed for both, vulvar cancer (De Hullu et al., 2000) and cervical cancer (Van de Lande et al., 2007).

Wydra et al. (2003) found that the superficial administration of the radiocolloid into the cervix provides a higher sentinel node detection rate compared to deeper administration, a fact we can strongly support. All the negative images in the learning phase apparently were due to inappropriate administration. In order to avoid false negative imaging, strictly intradermal injection combined with an at least 60 minutes follow-up and a trained, experienced team are key requirements. From the point of the application technique, both vulvar and cervical cancer are probably the most difficult ones. Hauspy et al. (2008) found that in vulvar cancer being at least 1 cm from the midline no contralateral sentinel node appeared, a finding we were unable to confirm. In contrast to Hauspy we found a higher rate of bilateral nodes (67.62% vs. 46%).

Overall, vulva sentinel shows excellent findings. Various authors with significant number of cases (Louis-Sylvestre et al., 2005, Moore et al., 2003, Hauspy et al., 2008, De Hullu et al., 2000) reported no false negative node. Vulva sentinel is probably one of the most relevant ones combining reduction of postoperative morbidity of these mainly older aged patients with excellent results.

 

REFERENCES

Basilio, P.; Da Fonseca, L. M. (2006), Sentinel lymph node detection in colorectal cancer: Importance, techniques and results. Arg Gastroenterol., 43, 163-167.         [ Links ]

Cabanas, R. M. (1977), An approach for the treatment of penile carcinoma. Cancer., 37, 456-466.         [ Links ]

Coleman, R. L.; Frumovitz, M.; Levenback, C. F. (2006), Current perspectives on lymphatic mapping in carcinomas of the uterine corpus and cervix. J Natl. Compr Cancer Network., 4, 471-478.         [ Links ]

Cox, C. E.; Pendas, S.; Cox, J. M.; Joseph, E.; Shons, A. R.; Yeatman, T. et al. (1988), Guidelines for sentinel node biopsy and lymphatic mapping of patients with breast cancer. Ann Surg., 227, 645-651.         [ Links ]

De Hullu, J. A.; Hollema, H.; Piers, D. A.; Verheijen, R. H.; van Diest, P. J.; Mourits, M. J.; Aaiders, J. G.; van Der Zee, A. G. (2000), Sentinel lymph node procedure is highly accurate in squamous cell carcinoma of the vulva. J Clin Oncol., 18, 2795-2797.         [ Links ]

Frisell, J.; Bergqvist, L.; Liljegren, G. et al. (2001), Sentinel node in breast cancer - a Swedish pilot study of 75 patients. Eur J Surg., 167, 179-183.         [ Links ]

Granegger, S.; Reinthaller, A.; Kainz, C.; Sliutz, G.; Sinzinger, H. (1999), Sentinel lymph node scintigraphy in patients suffering from vulva carcinoma. XVI Congr de la Asociacion Latinoam de Sociedades de Biologia y Medicina Nuclear. Buenos Aires, Abstract p. 53.         [ Links ]

Hauspy, J.; Beiner, M.; Harley, I.; Ehrlich, L.; Rasty, G.; Covens, A. (2008), Sentinel lymph node in vulvar cancer. Cancer, 112, 1869-1870.         [ Links ]

Hubalewska, A.; Sowa-Staszczak, A.; Huszno, B.; Markocka, A.; Pitynski, K.; Basta, A.; Oplawski, M.; Basta, P. (2003), Use of Tc99m-nanocolloid for sentinel node identification in cervical cancer. Nucl Med Rev., 5, 127-130.         [ Links ]

Hyde, N. C.; Prvulovich, E.; Keshtgar, M. R. (2002), A needle free system for cervical lymphatic mapping and sentinel node biopsy in oral squamous cell carcinoma. Oral Oncol., 38, 797-799.         [ Links ]

Kitagawa, Y.; Fujii, H.; Mukai, M.; Kubo, A.; Kitajima, M. (2005), Sentinel lymph node mapping in esophageal and gastric cancer. Cancer Treat Res., 127, 123-139.         [ Links ]

Louis-Sylvestre, C.; Evangelista, E.; Leonard, F.; Itti, E.; Meignan, M.; Paniel, B. J. (2005), Sentinel node localization should be interpreted with caution in midline vulvar cancer. Gynecol Oncol., 97, 151-154.         [ Links ]

Moore, R. G.; DePasquale, S. E.; Steinhoff, M. M.; Gajewski, W.; Steller, M.; Noto, R.; Falkenberry, S. (2003), Sentinel node identification and the ability to detect metastatic tumor to inguinal lymph nodes in squamous cell cancer of the vulva. Gynecol Oncol., 89, 475-479.         [ Links ]

Morton, D. L.; Wen, D. R.; Wong, D. H.; Economou, J. S.; Cagle, L. A.; Storm, F. K. et al. (1992), Technical details of intraoperative lymphatic mapping for early stage melanoma. Arch Surg., 127, 392-399.         [ Links ]

Sinzinger,H.; Meghdadi, S. (2001), Die Sentinel-Lymphknotenbiopsie aus der Sicht des Nuklearmediziners. Acta Chir Austriaca, 33, 5.         [ Links ]

Sliutz, G.; Reinthaller, A.; Lantzsch, T.; Mende, T.; Sinzinger, H.; Kainz, C.; Koelbl, H. (2002), Lymphatic mapping of sentinel nodes in early vulvar cancer. Gynecol Oncol., 84, 449-452.         [ Links ]

Valdés Olmos, R. A.; Tanis, P. J.; Hoefnagel, C. A.; Jansen, L.; Nieweg, O. E.; Meinhardt, W.; Horenblas, S. (2001), Penile lymphoscintigraphy for sentinel node identification. Eur J Nucl Med., 28, 581-585.         [ Links ]

Vande Lande, J.; Torrenga, B.; Riajmakers, P.; Hoekstra, O.; van Baal, M.; Brölmann, H.; Verheijen, R. (2007), Sentinel lymph node detection in early stage uterine cervix carcinoma: A systematic review. Gynecol Oncol., 106, 604-613.         [ Links ]

Wydra, D.; Sawicki, S.; Emerich, J.; Romanowicz, G. (2003), The influence of depth of marker administration on sentinel node detection in cervical cancer. Nucl Med Rev., 6, 131-133.         [ Links ]

 

 

Received: August 15, 2008;
Accepted: September 03, 2008.

 

 

*Author for correspondence

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