Antimicrobial activity and acetylcholinesterase inhibition by extracts from chromatin modulated fungi

ABSTRACT

Major health challenges as the increasing number of cases of infections by antibiotic multiresistant microorganisms and cases of Alzheimer's disease have led to searching new control drugs. The present study aims to verify a new way of obtaining bioactive extracts from filamentous fungi with potential antimicrobial and acetylcholinesterase inhibitory activities, using epigenetic modulation to promote the expression of genes commonly silenced. For such finality, five filamentous fungal species (Talaromyces funiculosus, Talaromyces islandicus, Talaromyces minioluteus, Talaromyces pinophilus, Penicillium janthinellum) were grown or not with DNA methyltransferases inhibitors (procainamide or hydralazine) and/or a histone deacetylase inhibitor (suberohydroxamic acid). Extracts from T. islandicus cultured or not with hydralazine inhibited Listeria monocytogenes growth in 57.66 ± 5.98% and 15.38 ± 1.99%, respectively. Increment in inhibition of acetylcholinesterase activity was observed for the extract from P. janthinellum grown with procainamide (100%), when compared to the control extract (39.62 ± 3.76%). Similarly, inhibition of acetylcholinesterase activity increased from 20.91 ± 3.90% (control) to 92.20 ± 3.72% when the tested extract was obtained from T. pinophilus under a combination of suberohydroxamic acid and procainamide. Concluding, increases in antimicrobial activity and acetylcholinesterase inhibition were observed when fungal extracts in the presence of DNA methyltransferases and/or histone deacetylase modulators were tested.

Keywords:
Antimicrobial activity; Acetylcholinesterase inhibition; Epigenetic modulation; Penicillium sp.; Talaromyces sp.

Introduction

Multiresistant microbial strains have emerged over the last decades^1,2 probably related to the extensive use of currently marketed antimicrobial agents. This scenario led to an increase on the search for novel sources of compounds with antimicrobial activity. Essential oils as lemongrass oil³ are an example of new source of compounds with antibiotic activity. In addition, screening of fungal extracts has demonstrated that Aspergillus and Penicillium species produce metabolites with antibacterial activity against potentially pathogenic bacteria species such as Staphylococcus aureus, Salmonella enterica serovar Typhimurium and Pseudomonas aeruginosa.^4,5

Alzheimer's disease is the most common cause of dementia and affected approximately 35 million people around the world in 2010.⁶ One of the mechanisms of Alzheimer's disease involves the reduction in acetylcholine production which implies in gradual loss of memory and learning ability along with other risk factors.⁷ Based on the cholinergic hypothesis it was discovered that some molecules inhibit acetylcholine degradation by acetylcholinesterase enzyme, increasing the time of action of acetylcholine in the synaptic cleft.⁸

Fungi are important sources of bioactive secondary metabolites.^9,10 It is believed that the fungi represent the second largest kingdom in terms of diversity.¹¹ Ascomycota phylum, in which the genera Penicillium and Talaromyces pertain, has a greater genetic diversity for secondary metabolites production when compared to other fungi groups.¹²

Besides antimicrobial activity, fungi are important sources of bioactive molecules with acetylcholinesterase inhibition activity.¹³ This high metabolite diversity observed in fungi could be due to the development of mechanisms for communication, competition and chemical defense over evolution process.¹⁴

Gene expression in filamentous fungi can be modulated by different conditions such as nutrients availability, light incidence, cultivation temperature, co-cultivation with other microorganisms, and epigenetic modifications.¹⁵ The term epigenetics refers to chemical modifications in chromatin structure that can be able to change the conformation of this molecule in order to affect gene expression.¹⁶ The basic structure of chromatin organization is given by the formation of nucleosomes, a protein octamer conglomerate (pairs of histone proteins H3, H4, H2A and H2B) in which the double-stranded deoxyribonucleic acid molecule is rolled.¹⁷ Heterochromatic regions are less active in terms of gene transcription when compared to euchromatic regions.¹⁸ Genes encoding secondary metabolites are usually organized in clusters¹⁹ and silenced genes expression can be activated using epigenetic approaches.

Small chemicals, such as hydralazine, suberohydroxamic acid and procainamide are used as inhibitors of enzymes related to epigenetic modification mechanisms, including DNA methyltransferases (DNMT) and histone deacetylases (HDACs). Suberohydroxamic acid acts as a HDACs inhibitor while other epigenetic modulators, e.g. procainamide, inhibit DNMTs.²⁰ Despite results showing that secondary metabolism is affected by epigenetic modulators, few evidences prove a change in qualitative and quantitative patterns of metabolites with biological potential after epigenetic induction of the producing microorganisms.

In this context, the goal of the present study was to evaluate the effect of epigenetic modulation on the antimicrobial activity and acetylcholinesterase inhibition by extracts from fungal growth media of species pertaining to the Penicillium and Talaromyces genera.

Materials and methods

Microorganisms and inoculum preparation

Five filamentous fungi species (Talaromyces funiculosus, Talaromyces islandicus, Talaromyces minioluteus, Talaromyces pinophilus, Penicillium janthinellum) were isolated from soil and stored at the Laboratory of Biotechnology and Bioassays (Federal University of Minas Gerais, Belo Horizonte, MG, Brazil). They were reactivated in tubes containing potato dextrose agar (PDA, Himedia Labs) for 7 days to promote sporulation. Five milliliters of aqueous Tween 80 0.5% (w/v) were added to the tubes containing the reactivated fungal strains and mixed to suspend spores. Spores suspension was standardized using a Neubauer chamber to obtain about 1 × 10⁷ spores mL^-1.

Epigenetic modulation

Suberohydroxamic acid, hydralazine and procainamide (Sigma-Aldrich, Darmstadt, Germany) were used as epigenetic modulators. All experiments were conducted in duplicate in Erlenmeyer flasks (500 mL) containing 125 mL of potato dextrose broth prepared in distilled water (PDB Himedia Labs) previously sterilized in autoclave. In a first group of experiments, epigenetic modulators were added individually (0.1 mM) before inoculation with 0.5 mL of pre-standardized spore suspension. The second set of experiments was conducted, also in duplicate, in which two epigenetic modulators were added together in the same concentration used in the first experiment (0.1 mM each). The following combinations were used: procainamide + suberohydroxamic acid; procainamide + hydralazine; and hydralazine + suberohydroxamic acid. As controls, experiments without addition of epigenetic modulators were run in parallel for each species and to exclude modulators interference in posterior chromatographic analysis, controls were carried without inoculation. Culture was carried out with shaking (100 rpm) at room temperature for 15 days. After this, liquid-liquid extraction was performed using ethyl acetate (25 mL) for three times. The organic solvent was evaporated down and the resulting extracts were kept in desiccator.

Chromatographic analysis of fungi extracts

Crude extracts were submitted to high performance liquid chromatography (HPLC UV/Vis - Shimadzu Prominence LC-20AT) in order to evaluate a possible effect on metabolite diversification promoted by epigenetic modulation by comparing the chromatographic profiles of the experimental and control assays. Methodology used was as described by Bracarense & Takahashi²¹ by injecting 20 µL of each extract (1 mg mL^-1) with a mobile phase consisting of water acidified with formic acid 0.1% (v/v) and methanol in a C18 column with linear gradient H₂O/methanol from 70:30 to 0:100 over 30 min. Elution was kept by 10 min in the final concentration with a total flow of 0.7 mL min^-1. Column was conditioned using the initial concentration of mobile phase for 15 min between sample injections. All samples were analyzed at 244 nm.

Antimicrobial activity evaluation

Extracts antimicrobial activities were evaluated by determining the sensibility percentage based on CLSI broth microdilution standard methodology²² against the following indicator strains: Escherichia coli ATCC 25922, Listeria monocytogenes ATCC 15313 and Candida albicans ATCC 18804. Extracts were diluted in dimethyl sulfoxide (DMSO). Extract concentration varied from 200, 100, 50, 25, 12.5, 6.26, 3.13 to 1.16 µg mL^-1 after microdilution. Final DMSO concentrations in the samples were kept under 5% (v/v). Ampicillin and miconazole were used as antibiotic and antifungal standards, respectively.

Acetylcholinesterase activity evaluation

Tests were conducted based on the Ellman spectrophotometric method modified by Rhee et al.²³ in 96-well microplates. Eserine was used as standard inhibitor (10 mg mL^-1), and control test was performed without the presence of acetylcholinesterase inhibitors. Experiments were performed in triplicate. Twenty-five microliters of acetylcholine iodide were dispensed in each well together with 125 µL of 5,5'-dithiobis 2-nitrobenzoic acid (DTNB); 50 µL of Tris-HCl buffer pH 8, 0.1% (w/v) bovine serum albumin and 25 µL of each extract (10 mg mL^-1) solubilized in DMSO. After a first reading at 405 nm, 25 µL of acetylcholinesterase (Electrophorus electricus) solution (0.222 U mL^-1) were added and then, nine readings were performed over a period of 10 min. The interference of spontaneous hydrolysis of the substrate was corrected using the average value of the inhibition absorbance (%) before addition of the enzyme. Inhibition of enzyme activity was calculated from the following equation: Inhibition % = [(C - A) × 100]/C, where C: control containing enzyme and substrate; A: test containing extract, enzyme and substrate.

Statistical analysis

Means were compared using a multivariable comparing Tukey test using Microsoft Excel 2016 software with Action Stat extension.²⁴ Differences were considered significant for p < 0.05.

Results

Regarding to the five fungal species evaluated in this study, it must be noted that the taxonomic classification of some of them has been recently modified by Yilmaz et al.,²⁵ transferring T. funiculosus, T. pinophilus, T. minioluteus and T. islandicus from Penicillium genus to Talaromyces genus. There are evidences that even close fungi can express only few taxon-specific secondary metabolites.²⁶ Epigenetic treatment of the filamentous fungi with three epigenetic modulators, individually or combined, led to the acquisition of extracts that were then analyzed by HPLC. In addition, antimicrobial activity and acetylcholinesterase inhibition of these extracts were determined.

Epigenetic modulation

The HPLC profile of the extracts obtained when suberohydroxamic acid, hydralazine and procainamide were added to the culture broth showed that these compounds act in different ways according to the fungal species utilized. In this study, hydralazine had a higher modulation effect compared to the other epigenetic agents used, showing stimulation of the metabolic differentiation of T. islandicus, T. funiculosus and T. pinophilus (Fig. 1A, D and E) when compared to the control profiles. Similarly, Williams et al.²⁷ related the production of new metabolites by Cladosporium cladosporioides and Verticillium psalliotae by hydralazine induction. P. janthinellum exhibited a modified profile after hydralazine or procainamide modulation (Fig. 1B), whereas for T. minioluteus, HPLC profile was modified only by exposure to procainamide (Fig. 1C).

Fig. 1
(i) Metabolic profile after individual epigenetic modulation SA, suberohydroxamic acid; H, hydralazine; P, procainamide. (A) T. islandicus; (B) P. janthinellum; (C) T. minioluteus; (D) T. funiculosus; (E) T. pinophilus. (ii) Metabolic profile after combined epigenetic modulation SA, suberohydroxamic acid. H, hydralazine; P, procainamide. (F) T. islandicus; (G) P. janthinellum; (H) T. minioluteus; (I) T. funiculosus; (J) T. pinophilus.

T. islandicus, T. funiculosus and T. pinophilus showed different response when submitted to epigenetic modulation with combined agents (Fig. 1F, I and J). Procainamide/hydralazine was the only combination able to promote high metabolic diversification in P. janthinellum (Fig. 1G). Inversely, treatment with suberohydroxamic acid/procainamide reduced metabolite expression under the conditions analyzed, highlighting that the balance between reactions of methylation and acetylation end up in complex results.

T. minioluteus exhibited similar metabolic profiles after exposure to both combinations of suberohydroxamic acid/hydralazine and hydralazine/procainamide (Fig. 1H). It must be pointed out that increase of metabolites production observed when the fungal species was grown in the presence of some epigenetic modulators, such as hydralazine, was resulting of the production of novel metabolites but also of preexisting metabolites. In this way, extracts that showed HPLC profiles more successfully affected by the epigenetic induction were assayed for their antimicrobial activity and acetylcholinesterase inhibition.

Antimicrobial activity

Screening of extracts from fungi, such as of Penicillium genus, has consistently demonstrated that these microorganisms are potential sources of molecules with wide antibacterial activity.⁹ However, none of the extracts tested in this work were active against E. coli. The extracts were also not active against C. albicans. By contrast, extracts obtained after exposure to hydralazine or procainamide showed higher inhibition potential against L. monocytogenes growth (Table 1) when compared to the control extract (15.38 ± 1.99%). L. monocytogenes growth inhibition was more pronounced under extract concentration equivalent to 200 µg mL^-1. Considering that only the extract of T. islandicus treated with hydralazine promoted an inhibition superior to 50%, it was not possible to determine the minimal inhibition concentration of the extracts. Nevertheless, antimicrobial activity potential of the extracts at the concentration of 200 µg mL^-1 is presented in Table 1. The most significant increases in inhibition, when comparing extracts obtained in the absence and in the presence of epigenetic modulators were observed with extracts from T. islandicus treated with hydralazine (from 15.38 ± 1.99 to 57.66 ± 5.98%) and T. funiculosus treated with procainamide and hydralazine (from 31.10 ± 2.36 to 41.55 ± 2.15%).

Table 1
Effect of the extracts obtained from the treatments with SA, P and H, alone or in combination, over L. monocytogenes growth (%).

Species	Treatment
	Control	P	H	SA + P	SA + H	P + H
	Inhibition (%)
T. islandicus	15.38 ± 1.99c	-	57.66 ± 5.98a	39.54 ± 5.17b	35.53 ± 4.89b	36.73 ± 3.07b
P. janthinellum	32.67 ± 2.11a	36.97 ± 5.22a	-	32.191 ± 2.40a	30.52 ± 1.50a	39.50 ± 4.97a
T. minioluteus	32.48 ± 2.94ab	28.89 ± 1.87b	-	28.61 ± 1.58b	30.14 ± 1.29ab	36.73 ± 4.14a
T. funiculosus	31.10 ± 2.36bc	-	29.90 ± 1.22c	-	35.82 ± 1.53b	41.55 ± 2.15a
T. pinophilus	29.52 ± 3.36a	17.72 ± 3.56b	23.40 ± 0.79ab	29.52 ± 3.51a	-	-

• Control, absence of epigenetic modulators; SA, suberohydroxamic acid; P, procainamide; H, hydralazine. Test control: ampicilin, 95.03% ± 0.59 of inhibition. -, not analyzed. Extract concentration, 200 µg mL^-1. Different superscripts in a row differ significantly (p < 0.05).

Acetylcholinesterase inhibitory activity

Some epigenetic modulators used in the present study were also able to increase the expression of secondary metabolites with acetylcholinesterase inhibitory activity (Table 2). Without epigenetic modulation, T. minioluteus extract was extremely active (100% of inhibition) against acetylcholinesterase activity, followed by T. funiculosus (67.84 ± 1.89%) and T. islandicus (56.78 ± 5.07%). On the other hand, P. janthinellum and T pinophilum extracts exhibited low acetylcholinesterase inhibitory activity, of 39.92 ± 3.76% and 20.91 ± 3.90%, respectively.

Table 2
Acetylcholinesterase inhibition activity of the extracts prepared using epigenetic modulators (%).

Species	Treatment
	Control	P	H	SA + P	SA + H	P + H
	Inhibition (%)
T. islandicus	56.78 ± 5.07a	-	68.04 ± 5.49a	54.60 ± 5.47a	69.90 ± 1.10a	54.29 ± 5.05a
P. janthinellum	39.62 ± 3.76b	100a	-	0	54.13 ± 5.04b	100a
T. minioluteus	100a	100a	-	100a	100a	100a
T. funiculosus	67.84 ± 1.89b	-	92.79 ± 1.68a	-	74.91 ± 5.04ab	88.46 ± 4.55ab
T. pinophilus	20.91 ± 3.90c	46.99 ± 1.77b	83.30 ± 0.40a	92.20 ± 3.72a	-	-

• Control, absence of epigenetic modulators; SA, suberohydroxamic acid; P, procainamide; H, hydralazine. Test control, eserine 94.47 ± 2.45 of inhibition. -, not analyzed. Different superscripts in a row differ significantly (p < 0.05).

After epigenetic modulation, P. janthinellum extracts treated with procainamide increased significantly its acetylcholinesterase inhibition capacity, and T. minioluteus extract did not lose its total inhibitory ability. The high acetylcholinesterase inhibitory activity of T. minioluteus could be related to the pool of metabolites with retention times of 19 and 27 min (Fig. 1C and H) in the HPLC analysis. After procainamide induction, T. pinophilus had a significant increase in acetylcholinesterase inhibitory activity, as well as after exposure to suberohydroxamic acid (92.20 ± 3.72%) and the combination suberohydroxamic acid/hydralazine (83.30 ± 0.40%).

Discussion

Filamentous fungi are eukaryotic organisms that have their genetic content organized as chromatin. Chromatin organization can be modified by epigenetic agents modulating the diversity of genes transcribed. Methylations reduce gene transcription activity while acetylations increase gene transcription²⁸ rates (Fig. 2).

Fig. 2
Epigenetic modulation of chromatin structure prone to increase gene transcription rate. (A) Euchromatin, more compact; (B) epigenetic modulation; (C) hetereochromatin, more transcribed genes. Higher proportion of acetylation/methylation.

The HPLC profiles of the extracts obtained after epigenetic induction showed several effects on the metabolite production when compared to the extracts obtained without addition of inducers. When grown without epigenetic induction, T. islandicus, T. minioluteus and T. funiculosus exhibited a high basal (control) production of metabolites when compared to P. janthinellum and T. pinophilus (Fig. 1). It is well known that basal metabolism of filamentous fungi, grown under laboratorial conditions, shows a reduction of the expression of cryptic gene clusters and that epigenetics plays an important role in cryptic gene expression.²⁹

DNMT inhibitors, hydralazine and procainamide, were generally more effective metabolite modulators compared to suberohydroxamic acid, meaning that these modulators promoted the diversification of the HPLC profile or increase of the production of basal metabolites. This may be related to modulation of CpG islands, which are methylation hotspots constituted by cytosine-guanine residues.³⁰ Hydralazine and procainamide effectiveness must be related to the fact that promoter regions are rich in CpG islands,³¹ which are usually undermethylated and when DNMT inhibitors are applied the result is increasing in allowing gene expression. This is related to a structural characteristic of promoter regions, indeed. In addition to that, epigenetic modulation has a complex balance considering the rate of methylations and acetylations. According to Kondo et al.³² the different epigenetic modifications may play a synergic or an antagonist role in gene expression, which can explain the diversity of results described in the present study.

According to the acquired data, the lower susceptibility of E. coli to the extracts tested could be related to the higher resistance of Gram-negative bacteria to antibiotics due to lipopolysaccharides in their cell wall constitution.³³ Zutz and collegues²⁶ described an increase in antimicrobial activity of Aspergillus clavatus extracts after modulation with azacitidine, a DNTM inhibitor as procainamide and hydralazine. This same modulator was related to the activation of antimicrobial activity of Streptomyces coelicolor extracts against five bacterial strains.³⁴ Recently, Byrne and colleagues³⁵ demonstrated the occurrence of L. monocytogenes in vegetables and the resistance of some strains to tetracycline and penicillin G, important antibiotics commercialized. These finding raises the importance of the screening of new sources of antimicrobial products with potential activity toward L. monocytogenes as described in this work. Larsen and Knochel⁴ also demonstrated the susceptibility of pathogenic bacteria to Penicillium camemberti extracts, with a wide spectrum of activity. The inhibitory selectivity of the extracts obtained in the present study can be of interest depending on the objective of a therapeutic.

Acetylcholinesterase inhibitory activity screening also demonstrated different responses to epigenetic modulation and biological activity. For T. funiculosus, hydralazine seems to play an important role in modulation, as both hydralazine alone and combined with syberohydroxamic acid or procainamide led to high inhibitory activities (74.91 ± 5.04% and 88.46 ± 4.55% respectively). The greatest effect obtained by epigenetic modulation was observed for P. janthinellum extracts after exposure to procainamide alone or combined with hydralazine, reaching 100% of inhibitory activity when compared to 39.61 ± 3.76% in control condition. Interestingly, this effect was associated with a high diversification in the production of compounds with retention times of 9 and 13 min in HPLC profile. On the other hand, when cultivated with suberohydroxamic acid/hydralazine, the inhibitory activity reached only 54.13 ± 5.04% and dropped to 0% with suberohydroxamic acid/procainamide combined. Inhibitory reduction of this extract could be related to the lower metabolite production observed after treatment with suberohydroxamic acid/procainamide. Few information is available in the literature on the use of epigenetic modulation to obtain extract of filamentous fungi inhibiting acetylcholinesterase activity, but Talaromyces sp. extracts have been reported to possess acetylcholinesterase inhibition activity.³⁶

The present work showed the differential expression of cryptic genes of filamentous fungi by epigenetic modulation based on the comparison of metabolic profiles, suggesting that epigenetic modulation promoted the expression of cryptic genes or promoted distinct effects toward genes generally expressed under laboratorial conditions. Activation of silenced genes was more efficient with DNMTs inhibitors than with HDACs inhibitor used in this study, and the fungal species tested responded differently to each epigenetic treatment. After epigenetic modulation, some fungal extracts showed increased antibacterial activity, but only when L. monocytogenes was used as indicator strain, whereas inhibition of acetylcholinesterase activity was improved by extracts of T. islandicus, P. janthinellum and T. pinophilus. These epigenetic modifications of chromatin could be used to improve the production of bioactive compounds.

Acknowledgements

Authors thank Ms. Mirra Angelina Neres da Silva and Dr. Vany Perpétua Ferraz for technical support at the high-performance liquid chromatography laboratory at the Department of Chemistry of UFMG; and the support by grants from Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

References

• ¹
  McDermott PF, Walker RD, White DG. Antimicrobials: modes of action and mechanisms of resistance. Int J Toxicol. 2003;22(2):135-143.
• ²
  Nikaido H. Multidrug resistance in bacteria. Annu Rev Biochem. 2009;78:119-146.
• ³
  Silva C, Guterres S, Weisheimer V, Schapoval E. Antifungal activity of the lemongrass oil and citral against Candida spp.. Braz J Infect Dis. 2008;12(1):63-66.
• ⁴
  Larsen G, Knechel S. Antimicrobial activity of food-related Penicillium sp. against pathogenic bacteria in laboratory media and a cheese model system. J Appl Microbiol. 1997;83(1):111-119.
• ⁵
  Fawzy GA, Al-Taweel AM, Melake NA. In vitro antimicrobial and anti-tumor activities of intracellular and extracellular extracts of Aspergillus niger and Aspergillus flavus var. columinaris. J Pharm Sci Res. 2011;3(1):980-987.
• ⁶
  Querfurth HW, LaFerla F. Alzheimer's disease. N Engl J Med. 2010;364(4):77-78.
• ⁷
  Craig LA, Hong NS, McDonald RJ. Revisiting the cholinergic hypothesis in the development of Alzheimer's disease. Neurosci Biobehav Rev. 2011;35(6):1397-1409.
• ⁸
  Mesulam M. The cholinergic lesion of Alzheimer's disease: pivotal factor or side show. Learn Mem 2004;11:43-49.
• ⁹
  Petit P, Lucas EMF, Abreu LM, Pfenning LH, Takahashi JA. Novel antimicrobial secondary metabolites from a Penicillium sp. isolated from Brazilian cerrado soil. Electron J Biotechnol. 2009;12(4):8-9.
• ¹⁰
  Hussain MS, Fareed S, Ansari S, Rahman MA, Ahmad IZ, Saeed M. Current approaches toward production of secondary plant metabolites. J Pharm Bioallied Sci. 2012;4(1):10-20.
• ¹¹
  Wright GD. Molecular mechanisms of antibiotic resistance. Chem Commun. 2011;47(14):4055.
• ¹²
  Pusztahelyi T, Holb IJ, Pócsi I. Secondary metabolites in fungus-plant interactions. Front Plant Sci. 2015;6:1-23.
• ¹³
  Wang Z, Ma Z, Wang L, et al. Active anti-acetylcholinesterase component of secondary metabolites produced by the endophytic fungi of Huperzia serrata. Electron J Biotechnol 2015;18(6):399-405.
• ¹⁴
  Brakhage AA. Regulation of fungal secondary metabolism. Nat Rev Microbiol. 2013;11(1):21-32.
• ¹⁵
  Takahashi JA, Teles APC, De Almeida Pinto Bracarense A, Gomes DC. Classical and epigenetic approaches to metabolite diversification in filamentous fungi. Phytochem Rev. 2013;12(4):773-789.
• ¹⁶
  Reuter S, Gupta SC, Park B, Goel A, Aggarwal BB. Epigenetic changes induced by curcumin and other natural compounds. Genes Nutr. 2011;6(2):93-108.
• ¹⁷
  Jiang C, Pugh BF. Nucleosome positioning and gene regulation: advances through genomics. Nat Rev Genet. 2009;10(3):161-172.
• ¹⁸
  Bartova E, Krejci J, Harnicarova A, Galiova G, Kozubek S. Histone modifications and nuclear architecture: a review. J Histochem Cytochem. 2008;56(8):711-721.
• ¹⁹
  Fox EM, Howlett BJ. Secondary metabolism: regulation and role in fungal biology. Curr Opin Microbiol. 2008;11(6):481-487.
• ²⁰
  Lu D. Epigenetic modification enzymes: catalytic mechanisms and inhibitors. Acta Pharm Sin B. 2013;3(3):141-149.
• ²¹
  Bracarense AAP, Takahashi JA. Modulation of antimicrobial metabolites production by the fungus Aspergillus parasiticus. Braz J Microbiol. 2014;45(1):313-321.
• ²²
  CLSI (Clinical and Laboratory Standards Institute). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grows Aerobically - Document M7-A6 Wayne, PA: Clinical and Laboratory Standards Institute; 2003.
• ²³
  Rhee IK, Meent M, Ingkaninan K, Verpoorte R. Screening for acetylcholinesterase inhibitors from Amaryllidaceae using silica gel thin-layer chromatography in combination with bioactivity staining. J Chromatogr A. 2001;915(1-2):217-223.
• ²⁴
  Action Stat. São Carlos, SP, Brazil: Estatcamp; 2014 http://www.portalaction.combr/
  » http://www.portalaction.combr/
• ²⁵
  Yilmaz N, Visagie CM, Houbraken J, Frisvad JC, Samson RA. Polyphasic taxonomy of the genus Talaromyces. Stud Mycol. 2014;78(1):175-341.
• ²⁶
  Zutz C, Bandian D, Neumayer B, et al. Fungi treated with small chemicals exhibit increased antimicrobial activity against facultative bacterial and yeast pathogens. Biomed Res Int. 2014;:540292.
• ²⁷
  Williams RB, Henrikson JC, Hoover AR, Lee AE, Cichewicz RH. Epigenetic remodeling of the fungal secondary metabolome. Org Biomol Chem. 2008;6(11):1857-2020.
• ²⁸
  Irvine RA, Lin IGHC. DNA methylation has a local effect on transcription and histone acetylation. Mol Cell Biol. 2002;22(19):6689.
• ²⁹
  Lim FY, Sanchez JF, Wang CCCKN. Toward awakening cryptic secondary metabolite gene clusters in filamentous fungi. Methods Enzym. 2012;517:303-324.
• ³⁰
  Takai D, Jones PA. Comprehensive analysis of CpG islands in human chromosomes 21 and 22. PNAS 2002;99(6):3740-3745.
• ³¹
  Sharif J, Endo TA, Toyoda T, Koseki H. Divergence of CpG Island promoters: a consequence or cause of evolution? Dev Growth Differ 2010;52(6):545-554.
• ³²
  Kondo Y, Shen L, Issa JP. Critical role of histone methylation in tumor suppressor gene silencing in colorectal cancer. Mol Cell Biol. 2003;23(1):206-215.
• ³³
  Silhavy TJ, Kahne D, Walker S. The bacterial cell envelope. Cold Spring Harb Perspect Biol. 2010;2(5):1-17.
• ³⁴
  Kumar J, Sharma VK, Singh DK, et al. Epigenetic activation of antibacterial property of an endophytic Streptomyces coelicolor strain AZRA 37 and identification of the induced protein using MALDI TOF MS/MS. PLOS ONE. 2016;11(2):e0147876.
• ³⁵
  Byrne VV, Hofer E, Vallim DC, Almeida RC. Occurrence and antimicrobial resistance patterns of Listeria monocytogenes isolated from vegetables. Braz J Microbiol 2016;47(2):438-443.
• ³⁶
  Ngokpol S, Suwakulsiri W, Sureram S, et al. Drimane sesquiterpene-conjugated amino acids from a marine isolate of the Fungus Talaromyces minioluteus (Penicillium minioluteum). Mar Drugs. 2015;13:3567-3580.

Edited by

Associate Editor: Gisele Monteiro de Souza

Publication Dates

• Publication in this collection
  Jan-Mar 2018

History

• Received
  29 Sept 2016
• Accepted
  17 June 2017