SciELO - Scientific Electronic Library Online

 
vol.33 issue3Performance of nile tilapia fed with spray-dried or vat-dries bovine blood mealWater footprint of pigs slaughtered in the states of south-central Brazil author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

  • English (pdf)
  • Article in xml format
  • How to cite this article
  • SciELO Analytics
  • Curriculum ScienTI
  • Automatic translation

Indicators

Related links

Share


Acta Scientiarum. Animal Sciences

On-line version ISSN 1807-8672

Acta Sci., Anim. Sci. vol.33 no.3 Maringá July/Sept. 2011

https://doi.org/10.4025/actascianimsci.v33i3.10702 

Effect of phytase supplementation on performance, bone densitometry and carcass yield in broilers chicks

 

Efeito da adição da enzima fitase sobre o desempenho, densitometria óssea e rendimento de carcaça de frangos de corte

 

 

Otto Mack JunqueiraI; Karina Ferreira DuarteI*; Vinícius AssuenaI; Rosemeire da Silva FilardiII; Antonio Carlos de LaurentizII; Maria Fernanda Ferreira Menegucci PraesI

IDepartamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, Universidade Estadual Paulista "Júlio de Mesquita Filho", Via de Acesso Prof. Paulo Donato Castellane, 14884-900, Jaboticabal, São Paulo, Brazil.
II
Departamento de Biologia, Faculdade de Engenharia de Ilha Solteira, Universidade Estadual Paulista "Júlio de Mesquita Filho", Ilha Solteira, São Paulo, Brazil.

Correspondence to

 

 


ABSTRACT

An experiment was conducted to evaluate the performance, bone densitometry and carcass yield of broilers chicks, using different levels of phytase enzyme. Nine hundred and sixty male one-day-old broiler chicks were used. The birds were distributed in a completely randomized experimental design, involving five treatments and six replications of 32 chicks each. The treatments consisted of a control diet for each phase, and four other diets were formulated adding growing levels of the phytase enzyme (250, 500, 750 and 1,000 FTU of phytase kg-1 feed). When adding the phytase enzyme, the nutritional matrix was valued to guarantee the same nutritional levels as the control diet. In general, the addition of phytase enzyme determined a linear decrease on the performance of the birds. However, the performance obtained with the level of 250 FTU phytase kg-1 feed were no different from the control treatment. The best bone density results were observed in the control treatment with no phytase, and the highest leg and thigh yield were obtained at the level of 514 FTU phytase kg-1.

Keywords: bone characteristics, carcass yield, performance, phytase enzyme.


RESUMO

Um experimento foi realizado com o objetivo de avaliar a inclusão de diferentes níveis da enzima fitase, sobre o desempenho, a densitometria óssea e o rendimento de carcaça e partes de frangos de corte nas diferentes fases de criação. Foram utilizados 960 pintainhos de corte com um dia de idade, da marca comercial Ross, distribuídos em um delineamento inteiramente casualizado, com quatro tratamentos mais uma testemunha (quatro níveis da enzima fitase - 250, 500, 750 e 1.000 FTU de fitase kg-1 de ração + testemunha) e seis repetições de 32 aves cada. Ao adicionar a enzima fitase, a matriz nutricional da mesma foi valorizada para garantir os mesmos níveis nutricionais da dieta testemunha. Foram avaliados os índices de desempenho, de densitometria óssea e o rendimento de carcaça e de partes nas diferentes fases de criação. De maneira geral o aumento na inclusão da enzima fitase determinou declínio linear sobre o desempenho das aves. Entretanto, até o nível de 250 FTU de fitase kg-1 de ração não foram observadas diferenças expressivas em relação ao tratamento isento de fitase. Os melhores resultados de densitometria óssea foram observados no tratamento controle sem a adição da enzima fitase e o maior rendimento de coxa+sobrecoxa foi obtido com o nível de 514 FTU de fitase kg-1 de ração.

Palavras-chave: características ósseas, desempenho, enzima fitase, rendimento de carcaça.


 

 

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

 

 

References

AHMAD, T.; RASOOL, S.; SARWAR, M.; HAQ, A.; HSAN, Z. Effect of microbial phytase produced from a fungus aspergillus niger on biovailability of phosphorus and calcium in broiler chickens. Animal Feed Science and Technology, v. 83, n. 2, p. 103-114, 2000.         [ Links ]

ANGEL, R.; SAYLOR, W. W.; MITCHELL, A. D.; POWERS, W.; APPLEGATE, T. J. Effect of dietary phosphorus, phytase, and 25 – hydroxycholecalciferol on broiler chicken bone mineralization, litter phosphorus, and processing yields. Poultry Science, v. 85, n. 7, p. 1200-1211, 2006.         [ Links ]

BANKS, K. M.; THOMPSON, K. L.; JAYNES, P.; APPLEGATE, T. J. The effect of copper on the efficacy of phytase, growth, and phosphorus retention in broiler chicks. Poultry Science, v. 83, n. 8, p. 1335-1341, 2006.         [ Links ]

BHARATHIDHASAN, A.; CHANDRASEKARAN, D.; NATARAJAN, A.; RAVI, R.; EZHILVALAVAN, S. Effect of enzyme supplementation on carcass quality, intestinal viscosity and ileal digestibilities of broilers to nutrient reduced diet. Tamilnadu Journal of Veterinary and Animal Sciences, v. 5, n. 6, p. 239-245, 2009.         [ Links ]

BROZ, J.; OLDALE, P.; PERRIN-VOLTZ, A-H; RYCHEN, G.; SCHULZE, J.; SIMÕES NUNES, C. Effect of supplemental phytase on performance and phosphorus utilization in broiler chickens fed a low phosphorus diet without addition of inorganic phosphates. British Poultry Science, v. 35, n. 2, p. 273-280, 1994.         [ Links ]

CATALÁ-GREGORI, P.; GARCÍA, V.; HERNÁNDEZ, F.; MADRID, J.; CERÓN, J. J. Response of broilers to feeding low - calcium and phosphorus diets plus phytase under different environmental conditions: Body weight and tibiotarsus mineralization. Poultry Science, v. 85, n. 11, p. 1923-1931, 2006.         [ Links ]

CONTE, A. J.; TEIXEIRA, A. S.; FIALHO, E. T.; SCHOULTEN, N. A.; BERTECHINI, A. G. Efeito da fitase e xilanase sobre o desempenho e as características ósseas de frangos de corte alimentados com dietas contendo farelo de arroz. Revista Brasileira de Zootecnia, v. 32, n. 5, p. 1147-1156, 2003.         [ Links ]

COOPER, R. J.; GOWING, H. S. Mammalian small intestine phytase (EC 3.1.3. 8). British Journal of Nutrition, v. 50, n. 3, p. 673-678, 1983.         [ Links ]

COSGROVE, D. J. Inositol phosphates: their chemistry, biochemistry and physiology. New York: Elsevier Scientific Publishing Co., 1980.         [ Links ]

DENBOW, D. M.; RAVINDRAN, V.; KORNEGAY, E. T.; SELF, B. B.; HULET, R. M. Improving phosphorus availability in soybean meal for broilers by supplemental phytase. Poultry Science, v. 74, n. 11, p. 1831-1842, 1995.         [ Links ]

DENBOW, D. M.; GRABAU, E. A.; LACY, G. H.; KORNEGAY, E. T.; RUSSELL, D. R.; UMBECK, P. F. Soybeans transformed with a fungal phytase gene improve phosphorus availability for broilers. Poultry Science, v. 77, n. 6, p. 878- 88, 1998.         [ Links ]

EECKHOUT, W.; DE PAEPE, M. Total phosphorus, phytate-phosphorus and phytase activity in plant feedstuffs. Animal Feed Science and Technology,
v. 47, n. 1-2, p. 19-29, 1994.

JORDÃO FILHO, J.; VILAR DA SILVA, J. H.; FERNANDES, A. C.; TEIXEIRA, E. N. M.; RIBEIRO, M. L. G.; COSTA, F. G. P. Níveis de P disponível e de fitase na ração de frangos de 22 a 42 dias. Revista Brasileira de Ciência Avícola, v. 8, supl., p. 107, 2006.         [ Links ]

LAN, G. Q., ABDULLAH, N.; JALALUDIN, S.; HO, Y. W. Efficacy of supplementation of a phytase–producing bacterial culture on the performance and nutrient use of broiler chickens fed corn – soybean meal diets. Poultry Science, v. 81, n. 10, p. 1522-1532, 2002.         [ Links ]

LOUZADA, M. J. Q.; PELÁ, C. A.; BELANGERO, W. D.; SANTOS-PINTO, R. Metodologia para avaliação de densidade em imagem radiográfica. Revista Brasileira de Engenharia Biomédica, v. 14, n. 2, p. 37-47, 1998.         [ Links ]

MARTINEZ-AMEZCUA, C.; PARSONS, C. M.; BAKER, D. H. Effect of microbial phytase and citric acid on phosphorus bioavailability, apparent metabolizable energy, and amino acid digestibility in distillers dried grains with solubles in chicks. Poultry Science, v. 85, n. 3, p. 470-475, 2006.         [ Links ]

MOHANNA, C.; NYS, Y. Changes in zinc and manganese availability in broiler chicks induced by vegetal and microbial phytases. Animal Feed Science and Technololy, v. 77, n. 3-4, p. 241-253, 1999.         [ Links ]

MURATA, L. S.; OLIVEIRA, A. S. C.; CORDEIRO, C. D. C.; McMANUS, C. M.; REZENDE, M. J. M.; SANTANNA, A. P. Desempenho de frangos de corte consumindo dieta com diferentes níveis de fitase. Revista Brasileira de Ciência Avícola, supl. 8, p. 133, 2006.         [ Links ]

OLIVEIRA, M. C.; MARQUES, R. H.; GRAVENA, R. A.; BRUNO, L. D. G.; RODRIGUES, E. A.; MORAES, V. M. B. Qualidade óssea de frangos alimentados com dietas com fitase e níveis reduzidos de fósforo disponível. Acta Scientiarum. Animal Sciences, v. 30, n. 3, p. 263-268, 2008.         [ Links ]

ONYANGO, E. M.; BEDFORD, M. R.; ADEOLA, O. The yeast production system in which Escherichia coli phytase is expressed may affect growth performance, bone ash, and nutrient use in broiler chicks. Poultry Science, v. 83, n. 3, p. 421-427, 2004.         [ Links ]

PALLAUF, J.; HOHLER, D.; RIMBACH, G. Effekt einer zulage an mikrobieller phytase zu einer mais-soja-diät auf die scheinbare absorption von Mg, Fe, Cu, Mn und Zn sowie auf parametter des zinkstatus beim ferkel. Journal of Animal Physiology and Animal Nutrition, v. 68, n. 1, p. 1-9, 1992.         [ Links ]

PAYNE, R. L.; LAVERGNE, T. K.; SOUTHERN, L. L. A comparison of two sources of phytase in liquid and dry forms in broilers. Poultry Science, v. 84, n. 2,
p. 265-272, 2005.

PERSIA, M. E.; SAYLOR, W. W. Effects of broiler strain, dietary nonphytate phosphorus, and phytase supplementation on chick performance and tibia ash. The Journal of Applied Poultry Research, v. 15, n. 1, p. 72-81, 2006.         [ Links ]

QIAN, H.; KORNEGAY, E. T.; DENBOW, D. M. Utilization of phytate phosphorus and calcium as influenced by microbial phytase, cholecalciferol and the calcium:total phosphorus ratio in broiler diets. Poultry Science, v. 76, n. 1, p. 37-46, 1997.         [ Links ]

QIAN, H.; VEIT, H. P.; KORNEGAY, E. T.; RAVINDRAN, V.; DENBOW, D. M. Effects of supplemental phytase and phosphorus on histological and other tibial bone characteristics and performance of broilers fed semi-purified diets. Poultry Science, v. 75, n. 5, p. 618-626, 1996.         [ Links ]

RAVINDRAN, V.; CABAHUG, S.; RAVINDRAN, G.; SELLE, P. H.; BRYDEN, W. L. Response of broiler chickens to microbial phytase supplementation as influenced by dietary phytic acid and non-phytate phosphorus levels. II. Effects on apparent metabolisable energy, nutrient digestibility and nutrient retention. British Poultry Science, v. 41, n. 2, p.193-200, 2000.         [ Links ]

ROBERSON, K. D.; EDWARDS JR., H. M. Effects of 1,25-Dihydroxicholecalciferol and phytase on zinc utilization in broiler chicks. Poultry Science, v. 73,
n. 8, p. 1312-1326, 1994.

ROSTAGNO, H. S.; ALBINO, L. F. T.; DONZELE, J. L.; GOMES, P. C.; OLIVEIRA, R. F.; LOPES, D. C.; FERREIRA, A. S.; BARRETO, S. L. T. Composição de alimentos e exigências nutricionais. Tabelas brasileiras para aves e suínos. 2. ed. Viçosa: UFV, 2005.         [ Links ]

SAS-Statistical Analisys System. User's guide statistics. release 6.12. Cary: Statistical Analysis System Institute, 2002.         [ Links ]

SEBASTIAN, S.; TOUCHBURN, S. P.; CHAVEZ, E. R.; LAGUE, P. C. The effects of supplemental microbial phytase on the performance and utilization of dietary calcium, phosphorus, copper, and zinc in broiler chickens fed corn-soybean diets. Poultry Science, v. 75, n. 6, p. 729-736, 1996.         [ Links ]

TEJEDOR, A. A.; ALBINO, L. F. T.; ROSTAGNO, H. S.; LIMA, C. A. R.; VIEITES, F. M. Efeito da adição da enzima fitase sobre o desempenho e a digestibilidade ileal de nutrientes. Revista Brasileira de Zootecnia, v. 30, n. 3, p. 802-808, 2001.         [ Links ]

VIVEROS A.; BRENES, A.; ARIJA, I.; CENTENO, C. Effects of microbial phytase supplementation on mineral utilization and serum enzymes activities in broiler chicks fed different levels of phosphorus. Poultry Science, v. 81, n. 8, p. 1172-1183, 2002.         [ Links ]

VIVEROS, A.; CENTENO, C.; BRENES, A.; CANALES, R.; LOZANO, A. Phytase and acid phosphatase activities in plant feedstuffs. Journal of Agricultural and Food Chemistry, v. 48, n. 9, p. 4009-4013, 2000.         [ Links ]

WU, Y. B.; RAVINDRAN, V.; MOREL, P. C. H.; HENDRIKS, W. H.; PIERCE, J. Evaluation of a microbial phytase, produced by solid-state fermentation, in broiler diets. 1. Influence on performance, toe ash contents, and phosphorus equivalency estimates. Journal of Applied Poultry Research, v. 13, n. 3, p. 373-383, 2004.         [ Links ]

WYSS, M.; BRUGGER, R.; KRONENBERGER, A.; REMY, R.; FIMBELD, R.; OESTERHELT, G.; LEHMANN, M.; VAN LOON, A. P. Biochemical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): Catalytic properties. Applied and Environmental Microbiology, v. 65, n. 2, p. 367-373, 1999.         [ Links ]

 

*Author for correspondence
E-mail: karinafduarte@yahoo.com.br

Received on July 21, 2010.
Accepted on January 25, 2011.

License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

 

 

1 Natuphos® 10000: commercial brand of the company BASF S/A: Estr. Samuel Aizemberg, 1707 - Bloco C - 2. Andar, São Bernardo do Campo, São Paulo, CEP 09851-550.

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License