Resumo em Inglês:

ABSTRACT The objective of this study was to evaluate and compare two chemical preservatives in terms of their sample preservation capabilities, considering the individual bacterial count (IBC) and time and temperature variables. Samples were collected in expansion tanks in three commercial dairy farms located in the northwest of Rio Grande do Sul, characterized as G1: low IBC values, G2: average IBC values, and G3: high IBC values. The tanks were stored at three different temperatures (4, 10, and 25 °C) for 14 d. Samples supplemented with the preservative Azilat in G1 (lower IBC group) exhibited the best results at a temperature of 4 °C, whereas for G2 and G3, the results showed no statistically significant difference between temperatures 4 and 10 °C. The temperature 25 °C exhibited the worst results. For samples preserved with Azidiol, regardless of the studied group (G1, G2, and G3), the temperatures of 4 and 10 °C did not present a significant difference regarding the preservation of the samples, with the temperature of 25 °C exhibiting the worst results. Azilat was effective in keeping the samples conserved when they presented low IBC, being able to fluctuate with the increase in IBC and temperature variation. Azidiol was effective regardless of the initial IBC level.

Resumo em Inglês:

ABSTRACT The objective of the present study was to evaluate the qPCR for detection and enumeration of Staphylococcus aureus and Streptococcus agalactiae using different milk samplings in comparison to the conventional microbiology. Four dairy herds with a history of subclinical mastitis caused by S. aureus and S. agalactiae were selected. Sampling approach included milk samples from bulk tank (BT), cow level (composite samples, CO), and mammary quarter level (MQ) from 785 lactating cows. Three consecutive monthly milk samplings were carried out, totaling 3347 MQ milk samples, 912 CO, and 12 from BT. All collected milk samples were subjected to conventional microbiology and qPCR for detection and enumeration of S. aureus and S. agalactiae. The qPCR showed 71.5% of diagnostic sensitivity for S. aureus isolated from MQ milk samples, 71.8% for CO, and 50% for BT milk samples compared with conventional microbiology methodology. Taken together, the diagnostic sensitivity for S. agalactiae isolated from MQ milk samples was 90.2, 87.7 for CO, and 90.9% for BT milk samples. In general, the qPCR methodology enabled the detection of S. aureus and S. agalactiae, regardless of the type of milk sampling. The direct use of milk samples to estimate the counting of S. aureus by qPCR demonstrated lower sensitivity than the counting of S. agalactiae, which can be explained by the pathogen infection dynamics and differences in milk sample type.

Resumo em Inglês:

ABSTRACT The objective of this study was to evaluate the effect of milking hygiene practices, herd size, water hardness, and temperature-humidity index (THI) on the physicochemical and microbiological characteristics of raw milk, and standard plate count (SPC) in milking machines of dairy farms in the central region of Mexico. Data were collected from fifty-three dairy farms during one year. The evaluated effects included milking hygiene conditions (good, medium, poor), herd size (1-50, 51-100, 101-150, ≥151 heads), water hardness (soft or moderately hard), and THI (comfortable or stressful). The increase in milking hygiene produced greater milk yield (MY) and energy corrected milk (ECM) but lower protein content, and decreased the individual bacterial count (IBC) and somatic cell count (SCC). The MY, ECM, protein content, IBC, and SCC were higher on bigger farms. The use of soft water reduced MY, IBC, and SCC, but improved fat, lactose, total solids (TS), and non-fat solids (NFS). Heat stress negatively affected fat, protein, TS, NFS, acidity, freezing point (FP), SCC, and methylene blue dye reduction test. Poor milking hygiene contributes to higher SPC in milking machine parts. Water hardness and THI did not affect SPC in all milking machine parts. Proper milking hygiene practices, larger herd size, softer water, lower THI, and adequate cleaning and disinfection of the milking machine parts benefits the physicochemical and microbiological quality of the milk.

Resumo em Inglês:

ABSTRACT We sought to develop a typology describing structural, production, and socioeconomic characteristics of dairy systems that produce artisanal cheese and compare it with that of systems that produce only raw milk. Data on 204 raw milk producers and 58 artisanal cheese producers in Paraná State, Brazil, were collected through on-site surveys and subjected to descriptive analysis, factor analysis, and means tests. Descriptive analyses were applied to characterize the sample and artisanal cheese production processes. Factor analysis identified the following three typological components: system production capacity, herd breed and milking characteristics, and farmer social characteristics. Farmers were divided into two groups, as follows: non-cheese producers (NCP) and artisanal cheese producers (ACP). Groups of farmers were compared in terms of typological components. It was found that ACP have smaller structure and production scale and focus less on herd breed and practices for improving milk quality than NCP. These results suggest that artisanal cheese production is a strategy to add value to milk that does not meet institutional or market requirements for transactions with the dairy industry, providing a foothold for producers to remain in the dairy business. Groups of farmers (ACP and NCP) do not differ in social indicators of typology.

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ABSTRACT This study mapped cattle carcass lesions and associated the isoline maps with the occurrence factors of these lesions. Isoline maps were drawn by common kriging. The associated occurrence factors were the layout of the boards in the crowding tub (CT), characteristics of the hauling truck (general state and the presence of loose and/or sharp boards), broken boards in the corral (BBC), type of road covered (TRC), and animal falls upon unloading at the slaughterhouse (FAUS). The BBC showed a higher number of carcass lesions in the rib region. The data on FAUS and deterioration of hauling trucks fit an exponential model, with more carcass lesions in the rib and hindquarters, and plate regions, respectively. The data on spaced boards in the CT and TRC (mixed) showed greater carcass lesions in the rib region. Therefore, this method provides important information about the spatial distribution of lesions in bovine carcasses, and the drawing sheet used is adequate to represent such lesions.

Resumo em Inglês:

ABSTRACT The objective of this study was to assess behavior patterns in Brazilian farm milk prices. We employed a structural time series techniques model, the Unobserved Component Model (UCM), which is part of the family of State Space models, to assess the trend, seasonality, cyclical behavior, and impacts of exogenous regressors on aggregated farm milk price behavior in Brazil from January 2005 to December 2019. We tested five alternative models with different regressors using the monthly national average prices of milk paid to farmers. The fit of the models was assessed with Akaike information criterion and Bayesian information criterion. Predictions were assessed by the root mean squared error (RMSE), mean absolute error (MAE), and mean absolute percentage error (MAPE). All models demonstrated a high degree of accuracy. Trends, seasonality, and two cycles were statistically significant, with the trend and long-period cycle contributing the most to price variation. Exogenous factors such as feed cost and international dairy product prices also had significant positive effects on the level of Brazil’s farm milk prices. All models demonstrated a high degree of accuracy, which may indicate their usefulness for price forecasting and policy formulation.

Resumo em Inglês:

ABSTRACT The objective of this study was to evaluate the potential of improving the economic value of integrated crop-livestock systems (ICLS) compared to conventional systems specialized in monoculture. The experimental area was 16.02 ha, divided into 18 paddocks of 0.89 ha each, organized in a randomized block design, with three replicates and six models of production systems: crop system [corn ( Zea mays ) grain production], livestock system (beef cattle under grazing conditions), and four ICLS, identified as: ICLS-1, corn integrated with Marandu palisadegrass [ Urochloa brizantha (Hoechst. ex A. Rich.) R.D. Webster cv. Marandu (syn. Brachiaria brizantha cv. Marandu] sown simultaneously without herbicide; ICLS-2, corn and Marandu palisadegrass sown simultaneously with herbicide; ICLS-3, corn and Marandu palisadegrass with lagged sowing; and ICLS-4, corn and Marandu palisadegrass sown simultaneously, with herbicide in rows and between-rows of corn. We demonstrated the economic impact analysis combined with the risk optimization and discounted cash flow techniques based on Monte Carlo simulation, considering price and productivity uncertainties. The indicators of added value and return on investment of ICLS had an economic advantage compared with conventional systems. It was also found that ICLS needed a smaller operational area than conventional systems for the economic break-even point. Integrated systems provide lower financial and operational risk levels and greater economic value per hectare compared with conventional systems specialized in monoculture.

Resumo em Inglês:

ABSTRACT The effects of diets containing oilseeds were measured to evaluate the productive and economic parameters in the finishing of young, feedlot Nellore bulls. Twenty-four young Nellore bulls were used, with an initial body weight (BW) of 311.46±0.37 kg and 24 months of age, distributed into individual stalls (4×20 m) in a completely randomized design, totaling four treatments with six repetitions per treatment. Four diets (control, based on corn and soybean meal, and three diets containing cottonseed, soybean, and sunflower) were evaluated. Feed and orts were measured daily to calculate intake and costs. The dry matter intake of the control group was higher than soybean (10.64 kg/day), cotton (9.88 kg/day), and sunflower (9.30 kg/day) treatments, respectively. The cottonseed treatment showed the highest average neutral detergent fiber intake. There was a dietary effect of diets on average daily gain, total weight gain, and final weight. The soybean treatment showed the highest performance, total gain (232.55 kg), and final weight (544.38 kg). Oilseed intake can modify the fatty acids profile in the meat, decreasing its saturated fatty acid content. Whole soybean seed favors performance, improves feed efficiency, fatty acid profile, and fat distribution in the carcass, and can reduce production costs.

Resumo em Inglês:

ABSTRACT The main objective of this study was to evaluate the effects of phenotypic, genotypic, and auction characteristics that may influence the selling prices of bulls. Data from 1,540 Braford and 1,179 Brangus bulls sold at auctions in the state of Rio Grande do Sul, Brazil, were collected and evaluated individually. Body condition score (BCS), muscularity, frame, foreskin size, presence or absence of horns, and data regarding the auction such as name/venue, event edition, forms of payment, among others, were considered. Information such as weight at the time of sale, scrotal circumference (SC), expected profit differences, selection indexes, and date of birth were collected from the catalogs supplied at the auctions. To obtain the influence of the selected explanatory variables throughout the conditional distribution of bull prices, a quantile regression was used, and the quantiles were established as follows: 10th, 25th, 50th, 75th, and 90th. The buyers of Braford and Brangus bulls valued characteristics such as age, weight, and SC in relation to frame, muscularity, BCS, and foreskin size. In addition, the price behavior at different auctions was positively related to the operation time of the auction in this market and with the reputation of the seller. Regarding genetic variables of Brangus bulls, these had little or no influence on the selling price. There was a positive influence on the weaning index in the 90th quantile and on the final index in the 50th and 75th quantiles. Buyers of synthetic bull breeds at auctions value the phenotypic characteristics of bulls more than they do genetic characteristics, which may not reflect permanent gains in the cattle herd.

Resumo em Inglês:

ABSTRACT The objective of this study was to spatially estimate the technical efficiency of sheep farms and explore the factors affecting the technical efficiency. Research data were collected randomly from 328 selected sheep farms in Ankara, Antalya, Diyarbakir, Konya, Mersin, and Sanliurfa provinces in Türkiye. The meta-frontier technical efficiencies (MTE) were estimated by using a stochastic meta-frontier production model. The meta-frontier technology ratio (MTR) was used to calculate the distance from the individual frontier to the meta-frontier. The determinants of technical efficiency were explored by using the two-limit Tobit model. Research results showed that the technical efficiency level of sheep farms varied spatially. The MTE of sheep farms in Ankara, Antalya, Diyarbakir, Konya, Mersin, and Sanliurfa were 0.45, 0.24, 0.25, 0.36, 0.42, and 0.21, respectively. The MTR was the highest in Konya, while the smallest was in Sanliurfa. Research results also showed that the variables of family size, education level of the operator, liquidity, return on asset, and information on sheep breeding positively affected the technical efficiency level of sheep farms. In contrast, total capital and farmland were negatively affected. The study suggests strengthening the economic viability of sheep farms and increasing their knowledge of sheep breeding to increase the technical efficiency level of sheep farms.

Resumo em Inglês:

ABSTRACT A feeding trial was performed to assess dietary protein:lipid ratios for the grow-out phase of the bullfrog (Lithobates catesbeianus). Nine balanced isoenergetic diets were formulated, combining three different protein levels (300, 400, and 500 g kg−1) with three different lipid levels (50, 100, and 200 g kg−1), designated as P300/50L, P300/100L, P300/200L, P400/50L, P400/100L, P400/200L, P500/50L, P500/100L, and P500/200L. Additionally, a commercial fish feed, commonly used in Mexico to feed bullfrogs, was also tested during the experiment. Growth performance, animal performance parameters, carcass composition, and fatty acid profiles in muscle and liver were evaluated. The feeding trial results showed that all the experimental diets enhanced growth, feed conversion ratio, and frog-leg weight compared with the commercial diet. Bullfrogs had higher growth with 400 and 500 g kg−1 of dietary protein regardless of dietary lipid content. It was also notable that with the P500/200L diet, frogs doubled the weight of those fed the commercial diet. DHA, EPA, and total omega-3 fatty acids were double in muscle and two to eight times higher in the liver compared with the commercial diet. In all cases, the final proximal composition of carcass reflected the diet composition. It is suggested that a diet containing 400 g kg−1 of protein and 50 g kg−1 lipids (protein/lipid ratio: 7.4; gross energy: 18.2 MJ kg−1) is adequate for bullfrog performance during the grow-out phase to achieve market size in a shorter period, thus, reducing farming risks and production costs.

Resumo em Inglês:

ABSTRACT The objective of this work was to assess the performance of two aeration types, splash and blower, used on a commercial biofloc technology (BFT) farm of red tilapia, Oreochromis sp., and their effect on growth performance and water quality parameters. For this, red tilapia juveniles were randomly distributed into twelve tanks. Each tank had an independent aeration system, and two experimental groups were characterized: six tanks used splash aerators (SPL group; one per tank, 1 hp), and the remaining tanks used a blower aerator (BLW group) with the same potency (1 hp). Water quality parameters were registered daily, and after 90 days of commercial culture, we obtained final growth parameters for each batch (tank). We observed no statistical differences on growth parameters, while water quality parameters showed that dissolved oxygen and oxygen saturation were significantly higher for the SPL group. Therefore, this field study corroborated indications from prior research at laboratory conditions that, at a commercial scale, splash aerators are more adequate for the production of red tilapia in BFT conditions.

Resumo em Inglês:

ABSTRACT In this study, we aimed to determine the effect of Moringa oleifera essential oil (MOEO) supplementation to rations of Japanese quail ( Coturnix coturnix japonica ) grown under heat stress (HS) on some adipokine (visfatin, adiponectin, and chemerin), intestinal (citrulline), 3,5,3′-triiodothyronine (T3), thyroxine (T4), and thyroid-stimulation hormone (TSH) levels in the serum. For this purpose, 320 day-old male quail chicks were used. The study was conducted over 42 days, including a 7-d adaptation period and a 35-d experimental period. Eight trial groups were established, each including 10 quail: CT (control temperature, 25 ℃), MOEO 200, MOEO 400, MOEO 600, HSC (HS control), SMOEO 200, SMOEO 400, and SMOEO 600. Based on the results, a dose-dependent decrease was observed on days 21 and 42 in serum adiponectin and T3 in the stress and stress-free groups compared with the control group due to MOEO supplementation. The lowest decrease was observed in the MOEO 600 mg/kg dose group. In addition, an increase in stressed groups was observed when examining serum citrulline levels, while chemerin levels did not have a statistically significant effect. While the decrease in serum visfatin, T4, and TSH levels on the 21st day did not have a statistically significant effect, a significant effect was determined on the 42nd day. The addition of MOEO at 600 mg/kg to the ration may be beneficial in preventing intestinal damage and inflammation that may occur due to HS.

Resumo em Inglês:

ABSTRACT This study evaluated the behavior of broiler chickens subjected to thermal stress during different periods of the day and age of birds. A total of 180 one-day-old male broiler chicks were allocated to three chambers. Each chamber contained five boxes, with 12 chicks/box. Birds were assigned to a completely randomized design. The birds were filmed, and the images recorded every minute during the two periods: morning (from 08:00 to 09:00 h) and afternoon (from 17:00 to 18:00 h), at 7, 14, and 35 d of age. The frequency of the behavioral category (water intake, feed intake, resting, exploration, and comfort) was determined. Data were subjected to variance analysis for mixed models with rearing temperatures (cold, usual, and hot) and age of birds (7, 14, and 35 d) as subdivided plot, and periods of the day (morning and afternoon) as sub-subdivided plot. Birds at cold and usual temperatures showed higher feed intake than those at hot temperature in the morning. Birds exposed to cold and usual temperatures explored the environment less frequently at 35 d when compared with 14 d. However, chickens at hot temperature showed decreased exploration according to the increase of age. Birds spent more time feeding during the morning, regardless of age and rearing temperature. Also, during this period of the day, chickens spent most of the time exploring the environment. The frequency of feed intake and exploration decreases with the increase of age. The similarity of behavior between chickens reared at cold and usual temperatures may be due to a change in the thermal comfort zone of the birds. It suggests that the real thermal comfort temperature is between the two temperature ranges studied (cold and usual).

Resumo em Inglês:

ABSTRACT The present study investigated whether the same temperature-humidity index (THI) values under different conditions of air temperature and relative humidity (RH) would affect the thermoregulatory, nutritional, and behavioral responses of laying hens. One hundred twenty Hy-Line Brown laying hens (60-weeks-old) were divided equally in two environmental chambers: 26 °C with 70% RH (hRH75) and 30 °C with 30% RH (hT75) for 28 days. The two ambient environments (hRH75 and hT75) had an identical THI value of 75, calculated using an empirical formula for laying hens. Neither hRH75 nor hT75 affected rectal and body-surface temperatures and heart and respiratory rates. The concentration of volatile fatty acids in fecal excreta were altered by the thermal treatments. hT75 vs. hRH75 decreased the proportion of acetate and increased the proportion of propionate in fecal samples. hT75 vs. hRH75 lowered the digestibility of dry matter, crude protein, and neutral detergent fiber at 14 days. Thermal treatments did not affect heat stress-associated behavioral responses including feeding, drinking, panting, and wing elevation at any stage. Laying hens exposed to the same THI at different temperatures and RH exhibit equal physiological responses including rectal and body-surface temperatures, heart and respiratory rates, and behavioral responses. Nonetheless, high-temperature treatment (hT75; 30 °C and 30% RH) vs. low temperature treatment (hRH75; 26 °C and 70% RH) affects nutrient digestibility and gut metabolites, suggesting that there are negligible but discernable responses to temperature in the gut physiology.

Resumo em Inglês:

ABSTRACT The objective of this study was to assess the effect of Suanzaoren decoction on stress response of horses in transportation. A total of six male Ili horses were equally divided into Suanzaoren decoction treatment group (n = 3; basal diet supplemented with Suanzaoren decoction, three times/day) and control group (n = 3; basal diet, three times/day). After feeding for five days, all horses were transported for 8 h to simulate stress. Blood and serum samples were obtained before transport (BT), during transport (T), and after transport (AT). Results showed that there was significant interaction between road transport and dietary Suanzaoren decoction supplementation for the white blood cells (WBC), intermediate cell ratio (MID%), granulocyte ratio (GRAN%), alanine aminotransferase (ALT), glucose (GLU), aspartate transaminase (AST), creatine (CK), cortisol (COR), human growth hormone (HGH), adrenocorticotropic hormone (ACTH) and arginine vasopressin content, insulin (INS), and thyronine 4 (T4) in blood and serum. The highest WBC was found in T group. The highest GRAN%, ALT, GLU, AST, CK, T4, ACTH, and INS contents were observed in serum from AT groups, which was decreased by treatment. In conclusion, dietary Suanzaoren decoction supplementation did relieve horse transportation stress. This study provides a useful clinical therapy for relieving transportation stress of horses.

Resumo em Inglês:

ABSTRACT The study evaluates the influence of environmental enrichment on behavior, leucometry, and performance of commercial pigs. Thirty-two hybrid pigs (Landrace × Large White × Pietran), at 69 days of life, were divided into four groups and subjected to different environmental stimuli: concrete floor, with no enrichment (C); floor bedding with wood chips (CM); concrete floor, with mobiles (MO); floor bedding with wood chips and the presence of mobile (CM + MO). The study collected behavioral data of the animals during 84 days, through the growing and finishing phases, each lasting 42 days, assessing the animals’ position and behavior by the instantaneous scan sampling method. The animals received ad libitum water and a specially formulated feed following the nutritional requirements for growing and finishing phases. The study considered daily feed intake, daily weight gain, and feed conversion. Group CM in the growing phase showed prolonged standing position periods and demonstrated a greater exploration of their environment (2.1%). Groups MO and CM + MO, in the growing phase, demonstrated extended periods of interaction with the offered enrichments (10.8±2.1 and 9.1±2.8%, respectively). Piglets in the finishing phase housed in pens with floor bedding with wood chips and those housed in pens with floor bedding with wood chips and the presence of mobile (tires and chains) showed longer interaction time with enrichments (4.8 and 5.4%, respectively), compared with the other groups C and Mo. Group C remained overall the longest in a standing position in both breeding phases. There was no effect on the animals’ leukogram. Environmental enrichment with wood shavings (as beddings) and hanging mobiles improves behavioral aspects of piglets in the growing and finishing periods.

Resumo em Inglês:

ABSTRACT This study integrated four microarray datasets by Robust Rank Aggregation (RRA) method to identify the differentially expressed genes (DEG) in bovine mammary epithelial (BME) cells in response to Escherichia coli and Staphylococcus aureus infection. Furthermore, the GO function and KEGG pathway enrichment analysis of the integrated DEG were performed. Finally, the protein-protein interaction (PPI) network was constructed. A total of 72 integrated DEG were identified from the four datasets. The most significantly enriched terms within the integrated DEG were mainly involved in the immune response. The PPI network of DEG was constructed with 53 nodes. Seventeen genes, which constitute a significant module, were identified as hub genes. Among them, CD40, CXCL6, and NFKBIZ were further screened as the key genes and have the potential to become biomarkers of E. coli and S. aureus mastitis, considering the specificity of biomarkers for diseases. The identified key genes and pathways in this study can assist in the search for biomarkers for mastitis diagnosis and disease resistance breeding.

Resumo em Inglês:

ABSTRACT To characterize the N6-methyladenosine (m6A)-related gene expression profiles in various tissues of Meishan pigs at different stages, m6A modification-related genes (METTL3, METTL14, METTL16, WTAP, RBM15, and FTO) were detected from newborn to physical maturity of Meishan pigs at eight important developmental stages (1, 7, 14, 21, 28, 35, 134, and 158 days old). The expression of m6A-related genes was tissue-specific. Furthermore, the level of METTL3 messenger RNA (mRNA) was higher on day 35 than in other stages in most tissues, and the expression of METTL14 increased after day 35, and FTO exhibited a peak on day 14 in muscle, intestine, lymph nodes, thymus, and kidney. This study provided a reference for an in-depth study of the expression patterns of m6A modification-related genes in Meishan pigs.

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ABSTRACT The objective of this study was to evaluate the effect of intronic single nucleotide polymorphisms (SNP) on temperament traits in a Brahman cattle population. The SNP located in CACNG4, EXOC4, NRXN3, and SLC9A4 candidate genes were genotyped in 250 animals with temperament records of exit velocity, pen score, and temperament score. Rs3423464051:G>A in the CACNG4 gene was associated with exit velocity and temperament score. An in silico analysis of the five intronic SNP showed that alternative alleles of CACNG4-rs3423464051, EXOC4-rs109393235, and SLC9A4-rs109722627 SNP could alter branch point sites during splicing, while a protein–protein interaction network analysis demonstrated a GRIA2 gene-mediated interaction between CACNG4 and NRXN3. The present results support previously reported evidence regarding bovine temperament-related candidate genes, particularly CACNG4, which is a confirmed candidate gene in need of more detailed analyses to reveal its role in temperament-related traits.

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ABSTRACT The objectives of this work were to estimate the genetic parameters for the traits longevity (LG) and accumulated milk yield at 305 days (MY305) using a bitrait animal model and the single-step GBLUP method and estimate the genetic gain for LG through direct and indirect selection for MY305. We used 4,057 records of first lactations of Murrah dairy buffaloes, collected between 1987 and 2020, belonging to six Brazilian herds located in the states Ceará, Rio Grande do Norte, and São Paulo and 960 animals genotyped using the 90K Axiom Buffalo Genotyping (Thermo Fisher Scientific, Santa Clara, CA) to estimate the genetic parameters. The heritability estimate was 0.25 for MY305 and 0.13 for LG. The genetic gain for LG was 0.13 months under direct selection, and 0.14 months under indirect selection, which results in a relative selection efficiency of 11% under selection for MY305 compared with the direct selection. The genetic correlation between the two traits was 0.77, indicating that animals with genetic potential for high MY305 tend to live longer. The genetic trends for MY305 and LG were 0.22 kg/year and 5.20 days/year, respectively, indicating a positive response, which reaffirms its relationship with the high genetic correlation between the two traits.

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ABSTRACT The objective of this study was to identify differentially expressed genes (DEG) between females and males in early bovine embryos. Superovulated cattle (n = 4) in the age span of 16–18 months were artificially inseminated with semen from the same bull that has been proven to be fertile. Blastocysts were collected by routine non-surgical uterine flushing on day 7 after insemination. This study determines the sex of embryos using a micro-injection pipette to collect a few blastomeres through the Zona pellucida. The remaining blastomeres were used for single-cell RNA sequencing (scRNA-seq) analysis on the Illumina platform, followed by differential expression analysis, Gene Ontology (GO) function analysis, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The criteria for identifying DEG will be set at |log2(Fold change)| ≥ 1 and P<0.05. A total of 8004 DEG were detected in female (Denoted as BLX, n = 3) and male (Denoted as BLY, n = 3) blastocysts. Transcripts highly expressed in the female embryos were related to catalytic activity, nucleotide binding, and catabolic process, while transcripts highly expressed in the male embryos were linked to oxidative phosphorylation, mitochondrion, and the ribosome. Nine genes that may be involved in blastocyst growth and development were screened, suggesting that their differential expression may be responsible for the differences in the development of male and female embryos. This study provides important information on potential genes and pathways associated with differences in early female and male embryonic development.

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ABSTRACT To explore the functions of Txnip and its mechanism in adipocyte differentiation, the preadipocytes were isolated from subcutaneous adipose tissue of three-day-old piglets and induced into adipogenic differentiation. The expression of Txnip and ChREBP was silenced, and the Txnip overexpression was achieved in the cells with transfection of the recombinant lentivirus strategies. Txnip silencing promoted the differentiation of porcine preadipocytes and PPARγ expression, and a PPARγ inhibitor reduced this facilitation. Instead, Txnip overexpression exerted a suppressive effect on the cell differentiation and PPARγ expression, and the PPARγ agonist offset this inhibition. High glucose stimulated the preadipocyte differentiation and expressions of ChREBP and Txnip. In contrast, Txnip expression was reduced by ChREBP silencing, suggesting glucose-regulated Txnip expression through the mediation of ChREBP. Moreover, the expressions of ChREBP and Glut4 induced by high glucose and glucose uptake of the cells were reduced by Txnip-overexpression, but increased by Txnip silencing, while these changes of Txnip did not alter their expressions under low glucose. Collectively, Txnip could be an inhibitor of porcine preadipocyte differentiation, which attenuated the adipogenesis through the negative feedback regulation on PPARγ. Txnip impaired the induction of glucose on the preadipocyte differentiation through decreasing Glut4 expression and glucose uptake and subsequent decrease of the expression and transcriptional activity of ChREBP.

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ABSTRACT This study aimed to determine the effects of relocation time (RT; Experiment 1) and storage time after relocation (ST; Experiment 2) on microbial population, fermentative characteristics, and chemical composition of corn silage. In experiment 1, corn silage was stored for 30 d, subjected to different RT (0–60 h), and stored again for 30 d. Thirty experimental silos were used in a completely randomized design, with three replicates per treatment. In experiment 2, after 150 d of ensiling, silage was removed from a bunker silo, exposed to air for 9 h, relocated to experimental silos, and stored for periods ranging from 0 to 128 d. Twenty-eight experimental silos were used in a completely randomized design, with four replicates per treatment. Relocation time had no effect on fungi counts and concentrations of lactic and propionic acids in corn silage but resulted in a significant increase in dry matter content. In experiment 2, dry matter recovery and concentration of non-fiber carbohydrates decreased in corn silage stored for more than 32 d after relocation. Exposure of corn silage to air during relocation for up to 60 h followed by 30 d of storage did not compromise the fermentation profile or nutritive value of the silage. Increased storage time of relocated corn silage (up to 128 d) consistently decreases its nutritional value. The storage period seems to have an increased impact on nutrient loss in relocated silage than the relocation period.

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ABSTRACT The objective of this study was to verify the repeatability of the expression of forage characters in intraspecific hybrids of Paspalum notatum Flügge to aid early selection. Across five harvests, plant height, tiller population density, leaf dry matter, stem dry matter, inflorescence dry matter, total dry matter, and growth habit were quantified for five parents, 189 hybrids, and a commercially available cultivar as a control (n = 195). Analysis of variance, principal components analysis, and structural analysis methods were used to determine the repeatability coefficients. The repeatability coefficients (ρ^) for all evaluated characteristics generated by the different methods were between 0.05 (ANOVA II) and 0.95 (PCACov). For most of the characteristics studied, repeatability coefficients and determination coefficients were considered high. The repeatability coefficients estimates obtained for the eight characteristics evaluated with the ANOVA I and II methods were almost always lower than those obtained by PCA and structural analysis methods. Based on the covariance matrix, the principal component method generated higher estimates than those produced by ANOVA or structural analysis. Assuming a minimum 80% reliability to verify the relative superiority of the hybrids across all assessed traits, the five harvests proved adequate for selecting the optimal plant materials to advance to the next phase of the breeding program. However, reliable early selection for leaf dry matter, leaf:stem ratio, and total dry matter required a minimum of two harvests. The genetic parameters (h2 and CVg) showed a favorable scenario for direct selection to increase forage production.

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ABSTRACT The objective of this study was to evaluate the effects of dietary supplementation with different levels of nano-selenium (NS) on growth performance, antioxidant activity, biochemical parameters, and selenium content in Landes geese. A total of 120 80-week-old healthy Landes geese (4.44±0.03 kg) were randomly assigned to three groups, each with four replicates of 10 birds. The control birds were fed a basal diet without further dietary supplementation (0.0 mg/kg of NS) and the two experimental groups were fed the basal diet supplemented with dietary NS at 0.2 or 0.4 mg/kg of feed. The results demonstrated that NS dietary supplementation had no significant effect on growth performance. Increased total superoxide dismutase activity in serum, breast muscle and liver, glutathione peroxidase level in serum and liver, and catalase in breast muscle and liver were observed for both NS supplemented groups. Additionally, reduced malondialdehyde in serum, breast muscle, and liver was detected in both NS-supplemented groups. Compared with the control, the birds fed diets supplemented with NS had lower concentrations of alanine aminotransferase, triglyceride, aspartate aminotransferase, and low-density lipoprotein cholesterol in serum, while high-density lipoprotein cholesterol was increased. Furthermore, increased selenium, especially in the liver, was found in groups with dietary supplementation of NS. These findings suggest the supplementation of NS in diets can improve antioxidant status, biochemical parameters, and tissue selenium content, although it has no significant effect on growth performance of Landes geese.

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ABSTRACT The objective of this study was to estimate the nutritional requirement of digestible methionine + cystine for Japanese quail during the growth phase and its residual effect on the laying phase. One experiment was conducted, including three phases: starter, from 1 to 14 days of age; grower, from 15 to 42 days of age; and laying, from 43 to 168 days of age. The experimental design was entirely randomized with five treatments (0.52, 0.64, 0.76, 0.88, and 1.00% of digestible methionine + cystine) and five replicates. In starter phase, 48 quail/experimental unit (box) were used; in grower phase, 31 quail/experimental unit (box) were used, which were reared until 14 days old, receiving conventional feed and, in laying phase, 12 quail/experimental unit were selected from grower phase to evaluate the residual effect. To evaluate animal performance, feed intake (g/bird), body weight (g), weight gain (g), feed conversion (g/g), and viability (%) were analyzed. At 14 and 42 days of age, the relative weight (%) of liver, spleen, and cloacal pouch organs, the birds’ warping (%) and body chemical composition (%) were evaluated. During laying, performance and egg quality were evaluated. In the starter and grower stages, quadratic effects were observed for all performance variables. Laying performance results confirmed the estimates obtained in the grower phase; however, there was no effect on egg quality. Considering the best feed conversion, the nutritional recommendation of digestible methionine + cystine for Japanese quail in the starter phase is 0.85% and in the grower phase, it is 0.77%, corresponding to daily intake of 50.43 and 158.5 mg of digestible methionine + cystine/day, respectively.

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ABSTRACT The objective of this study was to evaluate the effects of different levels of microencapsulated carvacrol and cinnamaldehyde on metabolizable energy, feed digestibility, intestinal morphometric analysis, and antioxidant parameters in broilers. A completely randomized design with five treatments and eight replications of eight broilers (Cobb male) was used, and collections were carried out at 20 and 42 days of age. Carvacrol and cinnamaldehyde concentrations (mg/kg) were: 0 (control), 50, 100, 150, and 200. Carvacrol and cinnamaldehyde improved apparent metabolizable energy but did not change protein and ether extract digestibility. Supplementation increased intestinal villus height and villi:crypt ratio; in which 100 mg/kg produced the greatest villus height. Serum uric acid levels were lower in birds receiving supplementation. Improvement in the activity of glutathione peroxidase and glutathione-S-transferase was observed, while lower uric acid, thiobarbituric acid reactive substances, and reactive oxygen species levels were observed. Microencapsulated carvacrol and cinnamaldehyde improve apparent metabolizable energy and can be administered in broiler feed without risk to the bird’s health. These supplements may serve as alternative products to aid the performance of commercial poultry.

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ABSTRACT This study was conducted to investigate the effects of amylin, a pancreatic hormone polypeptide, on egg quality traits in laying hens. A total of 40 birds (14 wk of age) were randomly divided into two treatments with two replicates, 10 birds in each replicate. Twenty birds were subcutaneously injected with amylin at 75 µg/kg every other day (treatment 1), and the remaining animals (20 hens) were given only water as the control group (treatment 2). Eggs collected from the two groups (140 eggs per group) were examined for several quality performance traits such as egg weight, specific gravity, shape index, eggshell strength, eggshell thickness, eggshell ash, eggshell weight, Haugh unit (HU), albumen index, yolk index, yolk color, and albumen height. The results indicated that amylin had a positive effect on some egg quality traits in laying hens. Specific gravity, eggshell thickness, eggshell weight, HU, albumen index, yolk index, and albumen height, which are all considered as important quality parameters from the consumer point of view, especially HU, were relatively improved in the amylin treatment group. These results showed that the injection of 75 µg/kg amylin has a significant effect on some egg quality traits. Considering that egg quality characteristics are crucial for the egg industry, this study can be a reference for the detailed investigation of the use of amylin in the different stages of egg production.

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ABSTRACT In this study, we evaluated how guanidinoacetic acid (GAA) addition in diets with various metabolizable energy (ME) contents affects the performance of broiler chickens. We also estimated the equivalence of GAA in ME. We distributed 1,280 one-day-old broilers in a completely randomized design with eight treatments, eight replicates, and twenty birds per experimental unit. Treatments were based on ME levels (2,775-2,875-2,975 kcal/kg; 2,850-2,950-3,050 kcal/kg; 2,925-3,025-3,125 kcal/kg; or 3,000-3,100-3,200 kcal/kg, from 1 to 7, 8 to 21, and 22 to 42 days of age) and the inclusion of GAA (0 or 600 mg/kg). Supplementation of GAA increased weight gain in broilers at an energy level of 2,908 kcal/kg and improved feed conversion ratio (FCR) at energy levels of 2,908 and 2,983 kcal/kg. There was a linear reduction in feed intake and an improvement in FCR of broilers with increasing levels of energy in diets, with and without GAA addition. Solving the equivalence equation, by applying each of the weighted average energy levels studied. indicates the GAA equivalence of 133, 103, 74, and 44 kcal/kg of diet. In conclusion, GAA supplementation improves broilers’ efficiency of energy use; the average ME equivalence of 600 mg/kg of GAA is 88.5 kcal/kg.

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ABSTRACT The objective was to compare deoxynivalenol (DON) concentrations in feed ingredients and commercial swine diets measured by enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC). Seventy feed ingredient samples consisted of corn, corn dried distillers grains with solubles, corn gluten feed, corn gluten meal, palm kernel expellers, rice bran, soy hulls, soybean meal, and wheat. Commercial swine diet samples (n = 92) were collected from 23 swine farms of varying regions in Korea and different growth stages of pigs. The DON concentration of all samples was determined in duplicate. Statistical comparisons were performed to compare the analytical methods (ELISA vs. HPLC), diet phases, and regions. The DON concentrations in most ingredients and all diets determined by ELISA method were greater than those determined by HPLC. The DON concentrations determined by the ELISA method were less than 1 mg/kg in all ingredients except corn dried distillers grains with solubles and corn gluten feed, and those determined by the HPLC were less than 0.5 mg/kg in all ingredients. The DON concentrations in complete diets did not vary by region or growth stages of pigs. The DON concentrations in most feed ingredients and commercial swine diets determined by ELISA method are greater than those determined by HPLC, but does not vary by regions in Republic of Korea or pig growth stages.

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ABSTRACT The objective of this study was to determine the energy values and metabolizability of different lipid sources in the diet of Japanese quail at the laying phase. The quail were distributed in a completely randomized design with ten replications of seven poultry per treatment, totaling six treatments: basal feed (control) and basal feed containing 10% soybean oil, corn oil, canola oil, sunflower oil, and poultry fat. The values of nitrogen-corrected apparent metabolizable energy (AMEn) and the metabolizability coefficient (MC%) were evaluated. No significant difference was found between the different lipid sources for AMEn (kcal/kg) and MC (%). The value of metabolizable energy corrected for nitrogen for soybean oil was 8,790 kcal/kg; 8,773 kcal/kg for corn oil; 8,784 kcal/kg for canola oil; 8,788 kcal/kg for sunflower oil; and 8,681 kcal/kg for poultry fat in laying Japanese quail. The digestibility coefficients were 93.88% for soybean oil, 93.53% for corn oil, 93.32% for canola oil, 93.74% for sunflower oil, and 93.06% for poultry fat.

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ABSTRACT The objective of this study was to evaluate whether the addition of the enzymes phytase and xylanase, isolated or associated, in the liquid form after feed pelletization could improve energy utilization and digestibility of calcium and phosphorus by broiler chickens. Three experiments were performed using 120 birds each, divided into five treatments with eight replicates per group (n = 3), identified as: experiment 1 (xylanase: control, 1000 IU, 1500 IU, 2000 IU, 2500 IU), experiment 2 (phytase: control, 500 FTU, 1000 FTU, 1500 FTU, 2000 FTU), experiment 3 (xylanase + phytase: control, 3000 IU + 500 FTU, 3000 IU + 1000 FTU, 3000 IU + 1500 FTU, 3000 IU + 2000 FTU). Samples for digestibility tests were collected at 14 to 21 days of age. Therefore, the inclusion of liquid phytase and liquid phytase + xylanase after pelletization in broiler diets has become a relevant way to reduce the inclusion of inorganic P, which can reduce the cost of feed and P excretion in the environment. Furthermore, it is an interesting strategy to avoid enzyme denaturation in the pelleting process.

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ABSTRACT The objective of this study was to evaluate the effects of replacing choline chloride with a plant source of choline on the locomotor system, liver health, and development of breast myopathies in broilers aged 1-42 days of age. We allocated 1,120 one-day-old Cobb broilers to four treatment groups and fed them commercial diets based on corn and soybean meal. The treatments included choline in the form of 1,800.00 mg/kg choline chloride; 1,350.00 mg/kg choline chloride + 450.00 mg/kg herbal choline; 900.00 mg/kg choline chloride + 900.00 mg/kg herbal choline; and 1,000.00 mg/kg herbal choline. Each treatment group had eight replications. Throughout the experiment, gait score, footpad dermatitis, hock burn, and leg deformities (valgus and varus) were evaluated in the birds at 28 and 35 days of age. After slaughter, parameters such as breast myopathies, tibial dyschondroplasia score, and histological slides of the pectoral muscle, liver, and proximal tibial epiphysis were assessed. The results demonstrated good hepatic and locomotor health in the broilers, as no classical signs of choline deficiency were observed. Statistical analyses indicated no significant differences between treatments in terms of liver and locomotor health, suggesting that broilers fed diets supplemented with the plant source did not experience choline deficiency. Additionally, no statistically significant differences were found between treatments regarding breast myopathies. Overall, the tested choline plant source can effectively replace choline chloride in broiler diets.

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ABSTRACT The objective of this study was to evaluate whether the addition of different levels of capsaicin in the diet of female turkeys has beneficial effects on growth performance and the antioxidant and oxidant status in serum and meat. A total of 150 female turkeys were distributed in a completely randomized design with three treatments with five replicates per treatment and ten birds per experimental unit. Treatments were identified as control (without additive); T400 – basal diet containing 400 mg/kg of pepper extract per kg of feed; and T800 – basal diet supplemented with 800 mg/kg of pepper extract per kg of feed. Growth performance was measured on days 1 and 20 of the experiment. Blood samples were collected at 20 days of the experiment for analysis of the oxidant and antioxidant status, and at 20 days, five birds were euthanized per treatment for the analysis of the oxidant and antioxidant status of the meat. The turkeys fed diet supplemented with capsaicin had lower feed intake and better feed:gain ratio. Turkeys in the T800 treatment showed a reduction in serum levels of reactive oxygen species (ROS) and thiobarbituric acid, and this same effect was observed for ROS in their breast meat. Capsaicin supplementation improves feed conversion and reduces feed intake without altering weight gain in female turkeys. Furthermore, the addition of 800 g/ton reduces lipid peroxidation and protein oxidation in the serum and reduces protein oxidation in broiler turkey meat.

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ABSTRACT The objective of this study was to investigate the effect of cottonseed peptides from in vitro degradation with protease, Enterococcus faecalis ( E. faecalis ), or both on the growth performance, nutrient digestibility, and health status of pigs. We used four diets containing non-degraded, protease-degraded, E. faecalis -degraded, or both-degraded cottonseed meal at 20% for growing phase (6 wk) and 16% for finishing phase (7 wk), corresponding to barrows, with six replicates of six pigs each. Results showed that the in vitro degradation of cottonseed meal increased proportions of peptides and organic acids and decreased nitrogen-free extract and phytate phosphorus. The degraded cottonseed meal increased feed intake, body weight gain, feed efficiency, apparent ileal digestibility of dry matter, energy, crude protein, Ile, Leu, Lys, Met, Cys, Ser, and Gly, and blood profiles of superoxide dismutase, glutathione peroxidase, catalase, IgA, and IgG. The diet containing cottonseed meal by dual degradation showed more pronounced effects on nutritional compositions, growth performance, nutrient digestibility, and serum parameters than the other diets. The data suggest that cottonseed meal dually degraded in vitro with enzymes and probiotics can improve its nutritional properties and application in animal feed.

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ABSTRACT We conducted an experiment to evaluate the effect of different inclusion rates and routes of administration of Ganoderma spp. on growth performance and gut morphology in broilers. We randomly assigned 320 one-day-old male broilers (Ross 308) to eight treatments with the same basal diet. Performance parameters were food intake (FI), body weight gain (BWG), and feed conversion ratio (FCR). Treatments were a basal diet (NC) with 55 ppm of bacitracin methylene disalicylate BMC (PC), basal diet with Ganoderma at 50 ppm, 100 ppm, and 150 ppm in drinking water (WG50, WG100, WG150), and feed (FG50, FG100, FG150). Body weight gain was higher for FG150 compared with NC. Treatment FG150 and PC had the best indicators of intestinal morphometry, showing significant differences on villi height to crypt depth ratio compared with other treatments. Ganoderma supplementation may be an alternative for the replacement of growth-promoting antibiotics because it offers comparable results to those generated by bacitracin methylene disalicylate (BDM).

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ABSTRACT The objective of this study was to examine the effects of replacement of an antibiotic (ANT, chlorohydroxyquinoline) in the diet of weaned piglets with a prebiotic composed of β-glucans, glucomannans, and mannan-oligosaccharides on growth performance, diarrhea occurrence (DO), hematological parameters, pH of digestive content, organ weights, intestinal epithelium morphology, and intestinal bacterial counts. A total of 120 piglets (weaned at 21 d old; 6.32±0.10 kg BW) were allocated in a randomized block design, with six pens replicates and four piglets per pen as experimental unit. Treatments consisted of diets with 0, 1,000, 2,000, or 3,000 mg kg−1 of prebiotic and a basal diet with 120 mg kg−1 ANT. No differences of treatments were observed on performance, DO, pH of digestive content, organ weights, and hematological parameters. Duodenal villus density (VD) increased linearly with the prebiotic levels. In the jejunum, the prebiotic level of 2,000 mg kg−1 resulted in a greater VD compared with the ANT. Enterobacteriaceae count in the ileum and total and lactic acid bacteria counts in the cecum responded quadratically to increasing prebiotic levels. The equations estimated the prebiotic levels of 2,125, 1,167, and 1,500 mg kg−1 to provide reduced counts of the respective bacteria (ileal enterobacteria: 6.47 log CFU g−1; total cecal bacteria: 6.70 log CFU g−1; and cecal lactic acid: 7.29 log CFU g−1). Salmonella spp. was not detected, whereas Escherichia coli and Lactobacillus spp. gene copies were unaffected by the treatments. Prebiotic levels or ANT do not enhance performance or health of weanling pigs, but the effects observed on VD and microbiota profile might be an indication of the potential benefits of these products in commercial farming conditions.

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ABSTRACT This report examines the effects of calcium anacardate (CA) on the morphology, histology, and proteomics of the testes and epididymal ducts of breeding quail. To that end, 22 140-day-old males were grouped in four treatments, each with different concentrations of CA: 0% (control), 0.25%, 0.50%, and 0.75%. The testicles of the animals were weighed and measured, and proteins from the testicles were separated by 1D SDS-PAGE and analyzed via mass spectrometry. Overall, 35 bands were identified in the testicular tissue gels of European quail. Of these, 13 were significantly different, with treatment 0.75% CA being different from the control in all bands. Significant differences among the mean heights of the seminiferous epithelium and diameters of the epididymal duct were observed. In the control group, the average height of the seminiferous epithelium was 71.2 µm, and in 0.75% CA, it was 45.6 µm. Supplementing feed with CA altered the expression of specific proteins, such as superoxide dismutase and heat shock proteins, of the seminal plasma of quail, in addition to facilitating histological alterations regarding the height of the seminiferous epithelium and the diameter of the epididymal duct, improving the reproductive parameters.

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ABSTRACT The objective of this study was to evaluate characteristics of the testicular parenchyma and vascular parameters of the pampiniform plexus obtained by ultrasound, semen quality parameters, and sperm freezability in Nellore bulls classified based on residual feed intake (RFI). Twenty-seven bulls (21.82±0.88 months of age) evaluated for feed efficiency were sampled for the study, including 15 with low RFI (−0.592±0.09 kg dry matter/day) and 12 with high RFI (0.792±0.10 kg dry matter/day). In ultrasound and Doppler assessment, the most efficient animals (low RFI) showed higher pulsatility and resistive indexes, as well as a tendency towards greater heterogeneity of the testicular parenchyma (0.625±0.032 vs. 0.508±0.032, 1.012±0.072 vs. 0.802±0.072, and 12.9±0.96 vs. 10.2±0.96, respectively, for low vs. high RFI). However, these animals tended to have lower peak diastolic velocity (5.19±0.50 for low RFI vs. 6.54±0.50 for high RFI). Analysis of fresh semen showed a lower percentage of minor defects in low RFI animals (2.67±1.19%) compared with high RFI animals (8.10±1.19%), without differences in the other parameters in fresh or thawed semen and after thermoresistance testing. Evaluation of flow cytometry parameters showed a higher quality of mitochondrial respiration in semen samples of low RFI animals (22.04±2.50%) compared with high RFI animals (12.29±2.71%). Therefore, although RFI exerts an effect on the Doppler parameters of the pampiniform plexus, it is not sufficient to affect the quality of fresh or thawed semen.

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ABSTRACT The objective of this study was to evaluate the relationships between preovulatory follicle (POF) and corpus luteum (CL) diameters, and POF and CL vascular perfusion with progesterone production, ovulation, and pregnancy in Nellore cows subjected to timed artificial insemination (TAI). Nellore cows (n = 201) were subjected to ovulation synchronization and later to ultrasound evaluation of POF and CL at the time of insemination (D0) and seven days later (D7), respectively. Females were divided into three categories according to the POF diameter assessed at the time of insemination: small (SF), medium (MF), and large (LF) follicles. The LF group had a greater number and intensity of pixels in the POF ultrasound exam compared with the SF group. The CL flow intensity and progesterone concentration were also higher in the LF group. The SF group showed lower flow intensity and lower ovulation rate compared with the others. When non-pregnant females were compared to pregnant ones, no difference was observed in any of the analyzed variables. The results show for the first time in Nellore cattle the relationship between the size of ovarian structures and blood flow (quantity and intensity) as well as the ability of the CL to produce progesterone. The intensity of the POF pixels proved to be relevant, demonstrating correlations with the size and flow of the CL, which were not found when evaluating only the number of pixels, thus revealing the importance of evaluating complementary characteristics of the flow.

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ABSTRACT The objective of this study was to evaluate the effects of cocoa meal in feed concentrate on the spermatogenesis in sheep. Twenty-five uncastrated males were divided into four groups and fed concentrated feed, supplemented with 0, 10, 20, and 30% cocoa meal, respectively, for 150 days. At the end of this period, the animals were slaughtered, the testicles were collected for histological slides, and testicular morphometric assessments were conducted, including cell type quantification in the seminiferous epithelium, intrinsic spermatogenesis yield, Sertoli-cell index, total number of Sertoli cells (TNSC), TNSC per testicular gram, daily sperm production (DSP), DSP per testicular gram, testicular sperm reserve (TSR), and TSR per testicular gram. Data were subjected to normality analysis using the Shapiro-Wilk test, followed by the tests for each condition, at a significance level of 5%. The administration of cocoa meal did not alter the germinal epithelial cell population, except for the number of type-A spermatogonia, which was lower in the control group than in the group that received 20% supplementation. A difference was found in the ratio between the number of type-A spermatogonia and primary spermatocytes in pre-leptotene/leptotene, and in the ratio between Sertoli cells and primary spermatocytes in pre-leptotene/leptotene, the control group and the group that received 10% cocoa meal significantly varied from each other, but not in relation to the other evaluated percentages. The TNSC, TNSC/g, DSP, and TSR levels were diminished in the group that consumed 30% cocoa meal. The TNSC, DSP, and TSR exhibited a negative quadratic tendency. Supplementation with 10% cocoa meal improved the TNSC, DSP, and TSR of sheep.

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ABSTRACT The objective of this study was to determine the impact of metabolic changes occurring in induced subclinical gestation toxaemia in ewes on metabolic parameters and vigour in the first 72 h of their lambs’ life. Fifty-one adult Corriedale ewes of known gestation date and fed on a natural pasture were randomly divided into four groups at day 145 of gestation. Group A (ewes with single pregnancies) and group C (ewes with twin pregnancies) were fed on a natural pasture throughout the trial; group B (ewes with single pregnancies) and group D (ewes with twin pregnancies) were subjected to 75% feed restriction for three days until they reached glycaemia and β-hydroxybutyrate (BHB) values indicative of subclinical gestational toxaemia. The birth-to-first station and birth-to-first suckling times of the born lambs were studied. Within the first hour after parturition and at 24, 48, and 72 h, glycaemia and BHB concentrations were determined in the blood of lambs, and body temperature and body weight were assessed at the same time. Relative weight gain at 72 h of age was calculated for all lambs. The BHB concentration determined at all times studied showed no difference among the groups. Subclinical gestation toxaemia induced in ewes subjected to feed restriction causes a decrease in the glycaemia of their lambs in the first hour of life; however, it does not cause an increase in ketone bodies. The metabolic changes induced in the mothers do not have negative effects on the vigour of the lambs at birth, as the weight, temperature, and the time it took for lambs to stand and suckle are not affected. However, they have a negative effect on relative weight gain in the critical period of the first 72 h of life.

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ABSTRACT This experiment was conducted to investigate the effects of lactic acid bacteria preparations on microbial diversity and community structure of calves. On days 1 and 7 of the trial period, feces were collected into sterile tubes and labeled (Day 1: control group D1DZ, experimental group D1SY, and Day 7: control group D7DZ, experimental group D7SY). Twenty Angus calves (150±10 kg) were selected and randomly divided into two groups of 10 calves each. The control group fed a basal diet. In addition to feeding the basal diet, the experimental group was given 15 mL lactobacillus preparation orally at 09:00 and 16:00 h every day. Calves were allowed free feeding and drinking water. All other feeding environments and management conditions remained consistent with the experiment lasting for seven days. At the end of the experiment, the fecal microflora of the calves was analyzed using 16S rRNA sequencing techniques. The 16S rRNA analysis data were processed using the Excel 2007 software and analyzed by the IBM SPSS statistical software (Statistical Analysis System, version 22). The Alpha diversity index analysis showed that the Chao and the Ace indices were significantly different after feeding supplemented with lactic acid bacteria. The PCA analysis showed that the fecal flora structure differed significantly after supplementation with the lactic acid bacteria preparation. Further analysis showed that the lactic acid bacteria increased Firmicutes, Patescibacteria, Rikenellaceae_RC9_gut_group, Clostridium_sensu_stricto_1, and prevotellaceae_UCG-003 in the feces. Therefore, we speculate that lactic acid bacteria preparations play an important role in animal production and are beneficial to the diversity of the fecal microflora of the calves.

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ABSTRACT The objectives were to evaluate the effects of monensin and virginiamycin, alone or combined, on supplemented Nellore cattle grazing tropical grass during the rainy season. Two experiments were conducted simultaneously to evaluate intake, digestibility, CH4 emissions, blood parameters, performance, and carcass characteristics (Exp. 1), and ruminal fermentation and relative abundance of ruminal microorganisms (Exp. 2). Animals (n = 92 Exp. 1 and n = 12 Exp. 2) were distributed in a completely randomized design and allocated in twelve paddocks composed of Urochloa brizantha (A. Rich.) Stapf. cv. Xaraés. A protein-energetic supplementation of 3 g/kg of BW per day was provided to all animals. Supplements were: without additives (WA), monensin alone at 80 mg/kg of product (MN), virginiamycin alone at 150 mg/kg of product (VM), and monensin (80 mg/kg of product) combined with virginiamycin (150 mg/kg of product; MNVM). Treatments did not affect intakes of total dry matter (DM), supplement DM, and nutrients. However, the intakes of forage DM and crude protein decreased in cattle fed MNVM compared with animals fed WA, MN, and VM. Total volatile fatty acids increased in animals fed VM. Ruminal NH3-N decreased, and pH increased in animals fed MN, VM, and MNVM. Relative abundance of total F. succinogenes and S. ruminantium decreased and R. flavefaciens increased in animals fed MN and VM at d 118. Treatments had no effect on enteric CH4 emissions. The average daily gain (ADG) and total gain were greater in cattle fed MNVM than in cattle fed MN. Combination of monensin and virginiamycin altered the rumen microbial populations but did not decrease enteric CH4 emissions. However, it decreased forage dry matter intake without altering the ADG and total weight gain, leading to an increase in feed efficiency. Results from this study indicate an advantage in including feed additives combined in the diet of supplemented Nellore cattle grazing tropical grass during the rainy season.

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ABSTRACT This study investigated oxidative damage and exocrine dysfunction of fetal pancreas caused by maternal nutritional restriction. Eighteen ewes carrying singleton fetus were randomly divided into control group (CG, ad libitum, 0.67 MJ ME/BW0.75/d, n = 6), restricted group 1 (RG1, 0.33 MJ ME/BW0.75/d, n = 6), and restricted group 2 (RG2, 0.18 MJ ME/BW0.75/d, n = 6) at d 90 of pregnancy. Maternal undernutrition was imposed from d 90 to 140 of pregnancy. At 140 d of gestation, fetal blood and pancreas tissue were collected to determine fetal pancreatic extracellular matrix, antioxidant capacity, and indicators of exocrine dysfunction. With the decrease of maternal nutrition, the fetal body weight, pancreatic weight, and DNA content were reduced in RG2 compared with CG, and increased and thickened collagen fibers were observed in RG2. Fetuses in RG2 exhibited increased collagen 3 (COL3) and fibronectin (FN) levels relative to CG, and the COL1:COL3 ratio was lower than that of the CG. For RG1, we found increased COL3 compared with CG. Malondialdehyde, serum amylase, and serum lipase in fetal pancreas in RG2 increased, but the total antioxidant capacity (T-AOC) decreased compared with the CG. The impaired ovine fetal pancreas growth, antioxidant imbalance, and pancreatic exocrine dysfunction are induced by maternal undernutrition during late pregnancy.

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ABSTRACT The objective of this study was to assess the effects of two levels of soybean hulls (0 and 400.1 g/kg) and whole or ground corn in the diet of newborn crossbred dairy male calves on intake, performance, blood indicators, and feeding cost. Twenty-eight calves with an average weight of 33.0±6.2 kg and four days of age were distributed into four treatment groups in a completely randomized design (n = 7) for 56 days. Weekly samples of feed, diets, and leftovers were collected to determine dry matter and nutrient intakes. To evaluate apparent digestibility, samples were taken using titanium dioxide as a maker. Blood samples were also collected to evaluate blood indicators. The inclusion of soybean hulls resulted in greater neutral detergent fiber intake by the calves, but reduced their non-fibrous carbohydrates intake, which was also reduced by the use of whole corn in the diet. Although the total digestible nutrients content of diets decreased with the use of whole corn and inclusion of soybean hulls, its intake did not vary (0.75 kg/d), regardless of the factors analyzed. The apparent digestibilities of dry matter (0.87 kg/kg) and crude protein (0.89 kg/kg) were similar, resulting in similar performance between the animals, regardless of the factors analyzed. In the quantities evaluated, the use of soybean hulls or whole corn did not affect blood indicators and was insufficient to reduce feed costs; the cost of daily feed was $2.06, while the cost per kilogram of gain was $3.74. The inclusion of up to 400.1 g/kg of soybean hulls or the replacement of ground corn with whole corn does not affect the performance of crossbred dairy calves during the preweaning period, demonstrating that both can be used in animal feed during this phase of production.

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ABSTRACT The objective of our study was to evaluate the effect of the use of legume (Arachis pintoi) or nitrogen fertilization on animal performance, characteristics of carcass and meat, and fatty acids profile of crossbred steers on Megathyrsus maximus cv. Aruana pasture, overseeded with temperate grasses. The experimental design was completely randomized, with three treatments and three replicates. The experiment was carried out from June to October (127 d). The treatments were: Low-N: 100 kg of N/ha; Medium-N: 200 kg of N/ha; and Legume: Arachis pintoi + 100 kg of N/ha. The pasture with higher nitrogen fertilization (N200) showed a more significant forage mass yield. The mixed grass with legumes presented a higher concentration of saturated fatty acids and saturated:unsaturated ratio in the meat. However, the grass pastures resulted in a higher content of unsaturated fatty acids in the meat. The other pasture variables, and characteristics of carcass and meat were not influenced by the treatments. The increase in nitrogen fertilization, from 100 to 200 kg/ha, and Arachis pintoi mixed with Aruana grass pasture overseeded with black oat and ryegrass does not affect the daily weight gain and the carcass and meat characteristics of the steers. The grass-legume mixture decreases the total concentration of unsaturated fatty acids in meat without influencing the concentration of polyunsaturated fatty acids.

Resumo em Inglês:

ABSTRACT The purpose of this study was to evaluate the performance of beef cattle with two levels of energy-protein supplementation of low-consumption on African Bermudagrass pasture, overseeded in winter with oat and ryegrass, with or without irrigation. Twenty-four castrated Angus steers (11 months old and had an initial average of 220 kg body weight (BW)) were used in experimental area of 3.6 ha. The experiment was completely randomized in a 2×2 factorial design, with three replicates. The evaluation period was 249 days (July/2019 to March/2020). The treatments were: irrigated pasture with supplementation of 1 g/kg BW or 2.7 g/kg BW and non-irrigated pasture with 1 g/kg BW of supplement or 2.7 g/kg BW. The grazing method was continuous with a variable stocking rate. Irrigation provided pastures with better chemical composition in winter and spring. Irrigation increased the daily accumulation rate in winter (84.6 vs. 45.9 kg DM/ha/day), providing a greater stocking rate (1,702 vs. 1,385 kg/ha) and, consequently, body weight gain per hectare. Supplementation of 2.7 g/kg BW provided a greater stocking rate in winter (1,652 vs. 1,435 kg/ha) and spring (3,096 vs. 2,811 kg/ha), not changing in summer. The association of irrigation and supplementation of 2.7 vs. 1.0 g/kg BW improves the intake pattern by the animal in summer without changing productivity parameters. Irrigation increases productivity and the nutritional value of pasture with higher livestock production per area in periods of water deficit. The supply of 2.7 vs. 1.0 g/kg BW provides a greater stocking rate and body weight gain per hectare.

Resumo em Inglês:

ABSTRACT The objective of this study was to evaluate the effect of either a limited forage intake or concentrate supplementation prior to the adaptation to high-concentrate diets on dry matter intake, ruminal pH, bacteria, and protozoa of Nellore cattle. The experiment was designed as a two 3×3 Latin square, and six cannulated Nellore steers were used. Each experimental period was composed by three feeding phases: pre-adaptation (14 days), adaptation (12 days), and finishing (seven days) diet, in a total of 33 days per period. The steers were assigned to one of three pre-adaptation dietary treatments: control (Tifton hay fed ad libitum + mineral supplement), restriction (Tifton hay fed at 1.4% of BW + mineral supplement), and concentrate (Tifton hay fed ad libitum + 0.5% of BW of a mix of concentrate feedstuffs and mineral supplement). The adaptation period consisted of two adaptation diets, which contained 72 and 79% concentrate for six days each. The finishing diet contained 86% concentrate. During the pre-adaptation phase, restricted cattle had higher pH than concentrate-fed cattle. There was a reduction in M. elsdenii relative population in cattle from either restriction or concentrate groups. During adaptation and finishing phases, cattle from concentrate group had smaller F. succinogenes populations compared with the control group. The previous nutritional backgrounds impact ruminal microbiota during adaptation and finishing phases without causing any negative effect on ruminal pH. Feeding concentrate prior to the adaptation positively impacted the transition to high-concentrate diets and promoted increased dry matter intake.

Resumo em Inglês:

ABSTRACT This study aimed to evaluate the effects of sunflower meal (SFM) inclusion on the performance, nutrient digestibility, and respirometric parameters in sheep. Twenty-four Dorper × Santa Inês uncastrated males, with an average age of 5 mo and initial body weight (BW) of 27.91±6.0 kg, were distributed in a randomized block design with four treatment diets containing 0, 100, 200, and 300 g of SFM/kg of total dry matter, replacing soybean meal. Intake, weight gain, ingestive behavior, and in vitro digestibility were evaluated according to the SFM inclusion. The respirometric parameters were evaluated in an open-circuit respirometric chamber. There was a quadratic response in dry matter intake expressed according to the SFM inclusion, with minimum points of 90.56 g/kg 0.75 BW for inclusion levels of 96 g of SFM/kg. A linear decrease in the in vivo digestibility of dry matter, neutral detergent fiber, and non-fibrous carbohydrate and in vitro fermentation of dry matter and fibrous carbohydrates was observed in response to SFM inclusion. These results reflect the higher fiber content of the byproduct, which reduced the non-fibrous carbohydrates in the diets. Despite the change in nutrient availability, average daily gain (299 g/day), O 2 consumption (26.24 L/kg 0.75 BW), and CH 4 production (2.25 L/kg 0.75 BW) were not influenced by the SFM inclusion, indicating that changes in the nutritional value of the diet did not affect the animals’ energetic metabolism. Sunflower meal inclusion decreased the digestibility but did not affect the animal gain and the respirometric parameters. The SFM can replace soybean meal in the diet of growing lambs; however, it has a maximum inclusion point of 88 g SFM/kg for feed conversion, which is a necessary factor for evaluating the replacement cost.

Resumo em Inglês:

ABSTRACT This study evaluated how nutritional insults applied at different stages of intrauterine development affect the growth and development of total tract organs and the mRNA expression of genes that encode growth factors, tight junction proteins, digestive enzymes, and glucose transporters in the small intestine of newborn goats. Fourteen nulliparous dairy goats were assigned to one of two nutritional plans over gestation: maintenance-restriction (M-R, n = 8) – 100% of maintenance requirement from d 8-84 of gestation followed by feeding at 50% maintenance requirement from d 85 of gestation to parturition; or restriction-maintenance (R-M, n = 6) – 50% of maintenance requirement from d 8-84 of gestation followed by feeding at 100% maintenance requirement from d 85 of gestation to parturition. Fisher's least significant difference test was performed using SAS 9.4, considering a full fixed-effect model. When expressed per kg of body weight, the weights of the small intestine and total intestine were 18.7 and 18.1% lower, respectively, for R-M offspring. The lengths of the small intestine and the total intestine of the R-M newborns were 19.1 and 9.5% shorter, respectively. This group also had a lower villus height:intestinal crypt depth ratio. The R-M offspring tended to have lower Solute carrier family 5 member 1 (SLC5A1), Solute carrier family 2 member 2 (SLC2A2), and Occludin (OCLN) mRNA expressions. Therefore, feed restriction in the first half of gestation is detrimental to the second half of gestation, reducing the offspring's intestinal absorptive area but enhancing their intestinal permeability.

Resumo em Inglês:

ABSTRACT The objective was to examine the effect of two short-term protein-energy supplementation (STPES) strategies at the rearing phase on the performance of Nellore calves grazing on Mombaça or Marandu grasses. The experiment used 72 calves (7-mo old, 229±3.0 kg body weight [BW]) allotted in a completely randomized block design to four treatments in a 2 × 2 factorial arrangement: two forage cultivars (Panicum maximum cv. Mombaça vs. Brachiaria brizantha cv. Marandu) and two STPES (WEAN – STPES immediately after weaning vs. TRAN – STPES in the transition of dry-rainy). The WEAN animals received 1 kg day−1 (n = 36) of a protein-energy supplement, whereas TRAN animals were subjected to STPES at 2 g/kg BW day−1 (n = 36), both for 53 days. Every 28 days, calves were weighed to measure performance, and pastures were sampled to evaluate productive and structural traits. Growth performance was analyzed considering a completely randomized block design with a 2 × 2 arrangement of treatment, whereas forage responses included repeated measures. Marandu had a higher forage mass (3,586 kg) than Mombaça (2,890 kg), but there was no difference in forage mass in each cultivar between preconditioning periods. The nutritional composition of Marandu and Mombaça cultivars did not differ and had similar results of in vitro fermentation variables. Stocking rate was higher for WEAN, in the Mombaça pastures. The STPES at weaning on Marandu provided greater gains per area. When applied in the period following the weaning and coinciding with the dry season, the STPES improves the performance of newly weaned calves kept on Marandu and Mombaça grasses.