Abstract Introduction Scientific studies in Brazil grew around 10.7% compared to previous years. However, the level of quality of evidence has been decreasing. The aim in our study is to examine the meeting abstracts of the Brazilian congress of coloproctology and analyze the level of evidence in trends and variables. Methods A descriptive bibliometric study, working with secondary data to review scientific abstracts in the annals of the coloproctology congress from 2015 to 2019. Results A total of 1756 abstracts of the Brazilian Congress of Coloproctology were analyzed for 5 years (2015-2019). There was a higher trend of abstracts presented with lower levels of evidence (level of evidence 5: 52.3% and 3: 30%), being the majority composed of case reports (49.4%) and retrospective studies (30.4%). The last two years analyzed (2018: 55.2% and 2019: 59.3%) had a predominance above average of case reports. From 2017 to 2019 there was a significant decrease in the number of level 2 evidence studies (18.10%,11.80% and 5.50%), while the number of studies with level 5 evidence showed an increase (45.60%, 56.60% and 61.40%). Statistical analysis occurred in only 17%, with an important decrease for the last two years (2018: 13.6%; 2019: 12.1%). Conclusions Although the data of this study is from the Brazilian coloproctology point of view, they are important for the global scientific community, as they allow a quantitative evaluation of the relative contribution from the level of evidence of Brazilian coloproctology researchers to the scientific scenario.

ABSTRACT Introduction The purpose of this retrospective bibliometric study was to assess the discrepancies between coloproctology surgery meeting abstracts and subsequent full-length manuscript publications. Methods Abstracts presented at the Brazilian Congress of Coloproctology Surgery from 2015 to 2019 were compared with matching manuscript publications. Discrepancies between the abstract and therefore the subsequent manuscript were categorized as major (changes within the purpose, methods, study design, sample size, statistical analysis, results, and conclusions) and minor (changes within the title, authorship, and number of female authors) variations. Results The conversion rate of abstracts in published manuscripts was 6,9% (121 abstracts). There were inconsistencies between the study title (66,1%), authorship (69,5%), study design (3,3%), sample size (39,2%), statistical analysis (24,8%), results (25,6%), and conclusions (12,4%) of manuscripts compared with their corresponding meeting abstracts. Conclusion As changes occur before manuscript publication of coloproctology surgery meeting abstracts, caution should be exercised in referencing abstracts or altering surgical practices based on abstracts content.

Abstract The presented development is an intelligent diagnostic system for transformers that studied machine learning techniques to determine the operational status of these transformers. The study of these techniques is initiated by observing the quantities that define the operational behavior of large transformers, aiming to identify anomalies in their operation from data from sensors that equipment it in the functioning environment. This large power transformer has a theoretical service life of above 20 years and a low failure rate. Thus, obtaining failure values, which have their evolution monitored for large transformers, is almost nil. Therefore, a supervised machine training methodology to diagnose these cases is practically unfeasible. The study carried out with several traditional intelligent techniques can verify this. Several supervised methods (Closest Neighbor K-th Neighbor, Support Vector Machine, Radial Base Function, Decision Trees, Random Forest, Neural Network, AdaBoost, Gaussian Naive Bayes, and Quadratic Discriminant Analysis) were studied.

Abstract As high-voltage circuit breakers (HVCBs) are responsible for switching off the load in the event of anomalies, they suffer various wear and tear, both on their main contacts and on the other actuation mechanisms. Not only load maneuvers but also weather conditions can bring factors that contribute to deterioration and, consequently, contribute to failures of this component that is so important for energy supply. Both failures and maintenance shutdowns generate costs for substations, something that could be minimized if there was monitoring of the condition of the HVCBs. This paper shows a methodology to analyze the vibration signal of HVCB in order to identify and quantify contact failures. The proposed methodology is verified through an experimental setup. The results show that it is possible not only to identify the fault but also to assess its intensity using vibration analysis.

ABSTRACT Introduction: the presentation of research at a congress is an interesting means for scientific dissemination, but only with publication in an indexed journal does the data become accessible and disseminated. The conversion rate in published articles of abstracts presented at congresses is an indicator to assess the scientific quality of those events. The aim of this study is to evaluate bibliometric characteristics of abstracts presented at the Brazilian Congress of Coloproctology and to determine the factors that affect publication rates. Methods: Retrospective evaluation of all abstracts presented at the Brazilian Congresses of Coloproctology from 2015 to 2019. Multiple databases were analyzed to estimate the conversion rate of the presented papers, as well as variables associated with the conversion of abstracts into full manuscripts through bivariate analysis and multivariate variables of these predictors. Results: 1756 abstracts were analyzed. Most studies are retrospective, series or case reports, and even personal experience. The conversion rate was 6.9%. The presence of statistical analysis was twice as high for published abstracts as for unpublished ones. Conclusion: the data presented demonstrate a low scientific productivity of the specialty, since the research carried out is, for the most part, not published as complete manuscripts. The predictors of publication of abstracts were: multicenter studies, studies with statistical analysis, study designs with a higher level of evidence and studies awarded by the congress.

RESUMO Introdução: a apresentação de pesquisas em um congresso é meio interessante para disseminação científica, porém apenas com a publicação em revista indexada é que os dados se tornam acessíveis e disseminados. A taxa de conversão em artigos publicados de resumos apresentados em congressos é um indicador para avaliar a qualidade científica de seus eventos. O objetivo deste estudo é avaliar características bibliométricas dos resumos apresentados no Congresso Brasileiro de Coloproctologia e determinar os fatores que afetam as taxas de publicação. Métodos: avaliação retrospectiva de todos os resumos apresentados nos Congressos Brasileiros de Coloproctologia dos anos de 2015 a 2019. Análise de múltiplas bases de dados para estimar a taxa de conversão dos trabalhos apresentados, assim como variáveis associadas à conversão dos resumos em manuscritos completos através de análises bivariadas e multivariadas desses preditores. Resultados: foram analisados 1756 resumos. A maioria dos estudos são retrospectivos, séries ou relatos de casos e até experiência pessoal. A taxa de conversão foi de 6,9%. A presença de análise estatística foi o dobro para os resumos publicados frente aos não publicados. Conclusão: os dados apresentados demonstram uma baixa produtividade científica da especialidade, já que as pesquisas realizadas não são publicadas, em sua maioria, como manuscritos completos. Os fatores preditores de publicação dos resumos foram: estudos multicêntricos, estudos contendo análise estatística, desenhos de estudo de maior nível de evidência e estudos premiados pelo congresso.

Abstract Previous studies have pointed to a possible relationship between vitamin D deficiency and the severity of the disease promoted by SARS-CoV-2, reducing respiratory and cardiovascular complications caused by a hyperreaction of the immune system known as “cytokine storm”. This vitamin exerts multiple functions that depend on the presence and levels of different proteins, such as the vitamin D receptor (VDR) and the vitamin D binding protein (DBP), and the existence of single nucleotide polymorphisms (SNPs) of the genes that encode these proteins. The objective of this review is to assess whether some VDR and GC SNPs are risk factors for the most severe forms of COVID-19 disease and whether they condition the response to vitamin D supplementation. A search was performed in PubMed, Google Scholar and Scielo, finding that genotypes in patients affected by COVID-19, were rarely performed, although some studies find a relationship between different alleles and the severity of the disease. The ApaI polymorphism of the VDR gene stands out, as the minor allele “a” increases the risk of mortality from COVID-19 (OR = 11.828, CI: 2,493-56,104, p = 0.002). RESULTS divergency in the efficacy of vitamin D supplementation suggest the need for a larger number of studies. In CONCLUSION, the study of VDR and GC polymorphisms seems essential to effectively treat vitamin D deficiency and particularly to protect against COVID-19. Well-designed studies are needed to elucidate whether plasma vitamin D levels play a role of casuality or causality.

Resumen Estudios previos han señalado una posible relación entre la deficiencia de la vitamina D y la severidad de la enfermedad promovida por el SARS-CoV-2, reduciendo las complicaciones respiratorias y cardiovasculares causadas por una respuesta exacerbada del sistema inmune. Esta vitamina ejerce múltiples funciones que dependen de la presencia y niveles de diferentes proteínas, como el receptor de la vitamina D (VDR) y la proteína de unión de la vitamina D (DBP), y de la existencia de polimorfismos de un solo nucleótido (SNP) de los genes que codifican a estas proteínas. El objetivo de esta revisión es evaluar si algunos SNP de VDR y GC son factores de riesgo de las formas más severas de la enfermedad COVID-19 y si condicionan la respuesta a la suplementación con vitamina D. Se realizó una búsqueda en PubMed, Google Scholar y Scielo, encontrándose que son escasos los genotipados en pacientes afectados por COVID-19, aunque algunos trabajos hallan una relación entre diferentes alelos y la severidad de la enfermedad. Destaca el polimorfismo ApaI del gen VDR, el cual alelo menor “a” aumenta el riesgo de mortalidad por COVID-19 (OR = 11,828, CI: 2.493-56.104, p = 0,002). La divergencia de resultados en la eficacia de la suplementación de vitamina D sugiere la necesidad de un mayor número de estudios. En conclusión, el estudio de polimorfismos VDR y GC resulta fundamental para tratar eficazmente la deficiencia de vitamina D y en particular en la protección frente a COVID-19. Se necesitan estudios bien diseñados para dilucidar si los niveles plasmáticos de vitamina D juegan un papel de casualidad o causalidad.

Resumo Objetivo Apresentar os resultados clínicos e radiográficos da correção cirúrgica de hálux valgo utilizando quatro técnicas percutâneas escolhidas de acordo com uma classificação radiográfica predefinida. Métodos Avaliamos prospectivamente 112 pés em 72 pacientes com hálux valgo operado em um período de um ano. A liberação de tecido mole distal (LTMD) percutâneo e o procedimento de Akin (LTMD-Akin) foram realizados em casos leves. Em hálux valgo de leve a moderado com ângulo distal da articulação do metatarso acima de 10°, adicionamos a osteotomia de Reverdin-Isham (RI). Em casos moderados com incongruência articular, realizamos o chevron percutâneo (CHP). Finalmente, uma osteotomia proximal percutânea fixada (OPPF) com um parafuso, semelhante à de Ludloff, foi proposta em casos graves com ângulo intermetatarsal (AIM) acima de 17°. De acordo com esses critérios, foram realizados 26 LTMDs-Akin, 36 CHPs, 35 RIs e 15 OPPFs. O seguimento médio foi de 17,2 meses (12 a 36 meses). A média de idade em operação foi de 58,8 anos (17 a 83 anos), e 89% dos pacientes eram do sexo feminino. Resultados A média do ângulo de hálux valgo (AHV) pré-operatório e o AIM diminuíram de 21° para 10,2°, e de 11,2° para 10,3°, respectivamente, em casos de LTMD-Akin. Em casos de RI, a média do AHV diminuiu de 26,6° para 13,7°, e o AIM, de 11,2° para 10,3°; em casos de CHP, o AHV médio diminuiu de 31° para 14,5°, o AIM diminuiu de 14,9° para 10,7°, e a OPPF, de 39,2° para 17,7°, e o AIM 11,8° para 6,8°. A média do escore de tornozelo e retropé da American Orthopaedic Foot and Ankle Society (AOFAS) aumentou de 49,2 para 88,6. A taxa de complicação foi de 11%. Conclusão Nosso protocolo de tratamento não difere muito dos clássicos, com resultados semelhantes. Temos como vantagem menos agressividade aos tecidos moles e melhores resultados cosméticos. Nível de evidência: nível IV, série de casos prospectivos.

Abstract Objective To present the clinical and radiographic results of hallux valgus surgical correction using four percutaneous techniques, chosen according to a predefined radiographic classification. Methods We prospectively evaluated 112 feet in 72 patients with hallux valgus operated over the course of 1 year. Percutaneous distal soft tissue release (DSTR) and the Akin procedure (DSTR-Akin) were performed in mild cases. In mild to moderate hallux valgus with distal metatarsal joint angle > 10°, we added the Reverdin-Isham (RI) osteotomy. In moderate cases with joint incongruity, we performed the percutaneous chevron (PCH). Finally, a Ludloff-like percutaneous proximal osteotomy fixed (PPOF) with a screw was proposed in severe cases with an intermetatarsal angle (IMA) > 17°. According to these criteria, 26 DSTRs-Akin, 36 PCHs, 35 RIs, and 15 PPOFs were performed. The mean follow-up was of 17.2 months (range: 12 to 36 months). The mean age at operation was 58.8 years (range: 17 to 83 years), and 89% of the patients were female. Results The mean preoperative hallux valgus angle (HVA) and the IMA decreased from 21° to 10.2° and from 11.2° to 10.3° respectively in the DSTR-Akin. In the RI, the mean HVA decreased from 26.6° to 13.7°, and the IMA, from 11.2° to 10.3°; in the PCH, the mean HVA decreased from 31° to 14.5°, and the IMA decreased 14.9° to 10.7°; as for the PPOF, the mean HVA decreased from 39.2° to 17.7°, and the IMA, from 11.8° to 6.8°. The average ankle and hindfoot score of the American Orthopaedic Foot and Ankle Society (AOFAS) increased from 49.2 to 88.6. The rate of complications was of 11%. Conclusion Our treatment protocol does not differ much from the classic ones, with similar results as well. We have as advantages less aggression to soft tissues and better cosmetic results. Level of Evidence: level IV, prospective case series.

RESUMO Objetivo: Descrever a primeira série de casos de transplante autólogo de condrócitos (TAC) em membrana de colágeno realizada no Brasil. Métodos: Doze joelhos de onze pacientes, com idade de 32,1 ± 10,9 anos, com lesões de cartilagem de espessura total do joelho de tamanho de 5,3 ± 2,6 cm 2 foram submetidos ao TAC, com seguimento mínimo de seis meses. Realizamos dois procedimentos cirúrgicos: biópsia artroscópica de cartilagem para isolamento e expansão de condrócitos, que foram semeados em uma membrana de colágeno implantada no leito da lesão. Foi realizada caracterização com imunofluorescência para colágeno tipo II (COL2) de células cultivadas e implantes, viabilidade celular e microscopia eletrônica no implante. Foram avaliados a segurança clínica, os escores funcionais KOOS e IKDC e a ressonância magnética. Utilizamos teste ANOVA para medidas repetidas, com comparações post-hoc, α = 5%. Resultados: COL2 foi identificado no citoplasma da célula, viabilidade celular foi superior a 95% e houve distribuição adequada e adesão celular na membrana. O seguimento mediano foi de 10,9 meses (7 a 19). Como complicações, ocorreram dois casos de artrofibrose, um de hipertrofia do enxerto e um de infecção superficial, nenhum deles havendo comprometimento da melhora clínica. Escalas KOOS e IKDC passaram de 71,2 ± 11,44 e 50,72 ± 14,10, no pré-operatório, para 85,0 ± 4,4 e 70,5 ± 8,0, aos 6 meses (p = 0,007 e 0,005). Ressonância magnética mostrou tecido regenerado compatível com cartilagem hialina. Conclusão: TAC em membrana de colágeno foi viável e seguro em seguimento de curto prazo, apresentando formação de regenerado visualizado através de imagens de ressonância magnética e melhora de função clínica. Nível de evidência IV, Série de casos.

ABSTRACT Objective: To describe the first series of cases of autologous chondrocyte implantation (ACI) in collagen membrane performed in Brazil. Methods: ACI was performed in 12 knees of 11 patients, aged 32.1 ± 10.9 years, with 5.3 ± 2.6 cm2 full-thickness knee cartilage lesions, with a six-month minimum follow-up. Two surgical procedures were performed: arthroscopic cartilage biopsy for isolation and expansion of chondrocytes, which were seeded onto collagen membrane and implanted in the lesion site; the characterization of cultured cells and implant was performed using immunofluorescence for type II collagen (COL2) for cell viability and electron microscopy of the implant. Clinical safety, KOOS and IKDC scores and magnetic resonance imaging were evaluated. We used repeated-measures ANOVA and post-hoc comparisons at α = 5%. Results: COL2 was identified in the cellular cytoplasm, cell viability was higher than 95% and adequate distribution and cell adhesion were found in the membrane. The median follow-up was 10.9 months (7 to 19). We had two cases of arthrofibrosis, one of graft hypertrophy and one of superficial infection as complications, but none compromising clinical improvement. KOOS and IKDC ranged from 71.2 ± 11.44 and 50.72 ± 14.10, in preoperative period, to 85.0 ± 4.4 and 70.5 ± 8.0, at 6 months (p = 0.007 and 0.005). MRI showed regenerated tissue compatible with hyaline cartilage. Conclusion: ACI in collagen membrane was feasible and safe in a short-term follow-up, presenting regenerated formation visualized by magnetic resonance imaging and improved clinical function. Level of evidence IV, Case series.

Resumen Introducción. La evaluación endoscópica del ámpula de Vater (AV), aunque recomendada como parte de la valoración rutinaria del tracto digestivo superior e incluso propuesta como un dato de certeza de un estudio completo, no siempre es posible debido a su localización y configuración anatómica que pueden ocultarla del campo visual del endoscopio frontal haciendo entonces necesario el uso del duodenoscopio. Dos estudios previos y la más reciente actualización de la Sociedad Europea de Endoscopia Gastrointestinal para la valoración duodenal y periampular en pacientes con síndromes polipósicos proponen como alternativa al duodenoscopio el uso de un cap distal en el endoscopio de visión frontal para facilitar la examinación del AV, reportando una efectividad del 91 al 95%. Objetivo. Determinar la factibilidad y eficacia del uso del endoscopio frontal asistido por cap para la valoración completa del AV. De manera secundaria se valora su morfología, el tiempo para realizar la localización correcta del AV, el tiempo total del estudio y los eventos adversos asociados al uso del cap. Material y métodos. Estudio prospectivo de un solo brazo realizado de agosto del 2019 a febrero del 2020. Se incluyeron de manera consecutiva los pacientes con indicación de endoscopia alta electiva de acuerdo con lo recomendado por las guías nacionales e internacionales vigentes. Se excluyeron a los pacientes con neoplasia avanzada, anatomía modificada por antecedente quirúrgico, diagnóstico establecido o sospecha de estenosis u obstrucción del tracto digestivo alto por clínica, endoscopia o estudios de imagen previos. La exploración endoscópica se realizó en todos los casos por o bajo la supervisión de un endoscopista experto certificado con un gastroscopio de visión frontal en cuya punta se colocó un cap plástico transparente de 4 mm de longitud. Se registraron los datos demográficos, la indicación del procedimiento, el diagnóstico final, el tiempo total intraprocedimiento en segundos (s), el tiempo de búsqueda una vez alcanzada la segunda porción duodenal (s), la visualización completa (bordes y orificio), incompleta/no visualizada del AV y su morfología clasificada en 4 tipos de acuerdo con lo propuesto por Haraldson y cols., en caso de poliposis duodenal se utilizó la clasificación de Spigelman. Se registraron los eventos adversos inmediatos y en las siguientes 72 horas. El análisis estadístico se realizó con el programa SPSS v23. Las variables continuas se describieron con medias, medianas y desviación estándar y las cualitativas con frecuencias relativas. Resultados: Se incluyeron 90 pacientes en el periodo establecido, de los cuales 36 fueron hombres (40%) y 54 mujeres (60%), la edad promedio fue de 52.4 (DE ±16.2) años. Un 15.5% contaba con el antecedente de un síndrome hereditario para cáncer de colon. El AV fue visible por completo en 98.8% (89/90) pacientes, en un paciente no fue posible identificarla a pesar de varios intentos y la evaluación por un segundo endoscopista. Se clasificó el AV como tipo 1 (clásica) en 49.4%, tipo 2 (plana) en 16.8%, tipo 3 (protuberante) 11.2% y tipo 4 (anillada) 22.4%, no se identificaron cambios ampulares neoplásicos en algún paciente, una paciente con síndrome de Lynch se encontró con poliposis duodenal (Spigelman IV). El tiempo promedio de búsqueda fue de 37.7 s (DE ±31.6), con un tiempo total procedimiento de 487.4 s (DE ±206.2). No se reportaron eventos adversos asociados a la técnica. Conclusiones. El uso combinado del endoscopio frontal y cap permitió la visualización completa y caracterización del AV en el 98.8% de los casos, con un tiempo promedio de búsqueda de 37 segundos y un tiempo total de procedimiento de 8 minutos, sin reportarse eventos adversos mayores. Su uso facilita la evaluación rutinaria del AV sin aumentar el tiempo recomendado de procedimiento ni las complicaciones de este. La utilidad de esta práctica puede ser aún mayor en pacientes con un riesgo aumentado de patología a este nivel (p. ej., poliposis adenomatosa familiar).

Abstract: Objective: To evaluate early childhood development (ECD) and its determinants in 12 to 59 months old children residents of communities <100 000 inhabitants. Materials and methods: The Encuesta Nacional de Salud y Nutrición of communities <100 000 inhabitants (Ensanut 100k) evaluated language level, access to ECD care services and standardized indicators of the eight quality of the development environment. We report indicator prevalence and standardized language scores according to variables of interest. Results: 20.7% of children attended eight well-child care visits within the first year of life, 13.0% received an ECD assessment, 75.0% receive support for learning, 23.4% have books and 57.7% experiment violent discipline. Improved language levels are associate with socioeconomic capacities, maternal education, preschool attendance, support for learning and household books. Children exposed to more protective factors present a language level 1.5 standard deviations higher than their peers exposed to more risk factors. Conclusion: There is a need to increase the coverage of ECD care services and to improve early development opportunities within households.

Resumen: Objetivo: Evaluar el desarrollo infantil temprano (DIT) y sus determinantes en niños/as de 12 a 59 meses residentes en localidades de menos de 100 000 habitantes. Material y métodos: La Encuesta Nacional de Salud y Nutrición en localidades con menos de 100 000 habitantes (Ensanut 100k) evaluó el nivel de lenguaje, acceso a servicios de atención al DIT e indicadores de calidad del contexto de desarrollo. Se estiman prevalencias de indicadores y puntajes estandarizados de lenguaje según variables de interés. Resultados: 20.7% de los niños/as asistió a ocho consultas del niño sano en su primer año, 13.0% recibió evaluación de DIT, 75.0% recibe apoyo al aprendizaje, 23.4% cuenta con libros y 57.7% sufre disciplina violenta. Mejores niveles de lenguaje se asocian con las capacidades económicas, escolaridad materna, asistencia a preescolar, apoyo al aprendizaje y acceso a libros. Los niños/as expuestos a más factores protectores presentan nivel de lenguaje 1.5 DE mayor que en niños/as con más factores de riesgo. Conclusión: Se requiere aumentar la cobertura de atención al DIT y mejorar las oportunidades de desarrollo en hogares.

Abstract Introduction and objective: The diagnostic and accuracy of fine needle aspiration biopsy assisted by endoscopic ultrasound (USE-FNAB) ranges between 68.4% and 84.3%, depending on several factors. The objective of this study is to demonstrate the safety, diagnostic performance and technical aspects involved in the adequacy of lymph node sampling by EUS-FNAB with emphasis on the use of suction. Material and methods: 38 lymph node biopsies were taken and analyzed for all variables that could have an impact on the final adaptation of the samples. A descriptive analysis was made by means and percentages, and an inferential analysis by parametric and nonparametric tests depending on the distribution and type of variable considering a statistical significance of p < 0.05. Results: Material was obtained in 100% of the studies, although only 73.7% of samples were reported adequate for diagnosis during the rapid cytology analysis, the final diagnostic yield was 84.2% after the review of the cell block (p = 0.031). In our study, there was no impact on the suitability of the sample when comparing the use of suction (55.3%) versus no suction (44.7%) (p = 0.258). Conclusions: The final review of the cell block increases the diagnostic yield of USE-FNAB in lymph nodes.

Resumen Introducción y objetivo: La precisión diagnóstica de la biopsia por aspiración con aguja fina asistida por ultrasonido endoscópico (USE-BAAF) oscila entre el 68.4 y el 84.3%, dependiendo de varios factores. El objetivo de este estudio es demostrar la seguridad, rendimiento diagnóstico y aspectos técnicos involucrados en la adecuación del muestreo de ganglios linfáticos por USE-BAAF con énfasis en el uso de succión. Material y métodos: Se tomaron 38 biopsias de ganglios linfáticos y se analizaron todas las variables que pudieran tener impacto sobre la adecuación final de las muestras. Se realizó un análisis descriptivo mediante medias y porcentajes, y un análisis inferencial mediante pruebas paramétricas y no paramétricas dependiendo de la distribución y tipo de variable, tomando en cuenta una significancia estadística de p < 0.05. Resultados: Se obtuvo material en el 100% de los estudios aunque solo se reportó un 73.7% de muestras adecuadas para el diagnóstico durante la revisión rápida de laminillas y un rendimiento diagnóstico final de un 84.2% después de la revisión del bloque celular (p = 0.031). En nuestro estudio no hubo impacto en la adecuación de la muestra al comparar el uso succión (55.3%) vs. no succión (44.7%) (p = 0.258). Conclusiones: La revisión final de bloque celular aumenta el rendimiento diagnóstico de la USE-BAAF en ganglios linfáticos.

Abstract: Objective: To evaluate early childhood development (ECD) and its determinants in 12 to 59 months old children residents of communities <100 000 inhabitants. Materials and methods: The Encuesta Nacional de Salud y Nutrición of communities <100 000 inhabitants (Ensanut 100k) evaluated language level, access to ECD care services and standardized indicators of the eight quality of the development environment. We report indicator prevalence and standardized language scores according to variables of interest. Results: 20.7% of children attended eight well-child care visits within the first year of life, 13.0% received an ECD assessment, 75.0% receive support for learning, 23.4% have books and 57.7% experiment violent discipline. Improved language levels are associate with socioeconomic capacities, maternal education, preschool attendance, support for learning and household books. Children exposed to more protective factors present a language level 1.5 standard deviations higher than their peers exposed to more risk factors. Conclusion: There is a need to increase the coverage of ECD care services and to improve early development opportunities within households.

Resumen: Objetivo: Evaluar el desarrollo infantil temprano (DIT) y sus determinantes en niños/as de 12 a 59 meses residentes en localidades de menos de 100 000 habitantes. Material y métodos: La Encuesta Nacional de Salud y Nutrición en localidades con menos de 100 000 habitantes (Ensanut 100k) evaluó el nivel de lenguaje, acceso a servicios de atención al DIT e indicadores de calidad del contexto de desarrollo. Se estiman prevalencias de indicadores y puntajes estandarizados de lenguaje según variables de interés. Resultados: 20.7% de los niños/as asistió a ocho consultas del niño sano en su primer año, 13.0% recibió evaluación de DIT, 75.0% recibe apoyo al aprendizaje, 23.4% cuenta con libros y 57.7% sufre disciplina violenta. Mejores niveles de lenguaje se asocian con las capacidades económicas, escolaridad materna, asistencia a preescolar, apoyo al aprendizaje y acceso a libros. Los niños/as expuestos a más factores protectores presentan nivel de lenguaje 1.5 DE mayor que en niños/as con más factores de riesgo. Conclusión: Se requiere aumentar la cobertura de atención al DIT y mejorar las oportunidades de desarrollo en hogares.

Resumo Objetivo: O diagnóstico da fibrose cística depende do valor da concentração de íons de cloreto no teste do suor (≥ 60 mEq/mL - reconhecido como o indicador-padrão para o diagnóstico da doença). Além disso, as glândulas salivares expressam a proteína RTFC igualmente às glândulas sudoríparas. Nesse contexto, nosso objetivo foi verificar a correlação da concentração de cloreto na saliva e a concentração de cloreto no suor e entre a concentração de sódio na saliva e a concentração de sódio no suor em pacientes com fibrose cística e indivíduos controles saudáveis, como uma ferramenta para diagnóstico de fibrose cística. Métodos: Contamos com a participação de 160 indivíduos [57/160 (35,70%) com fibrose cística e duas mutações no gene RTFC conhecidas e 103/160 (64,40%) indivíduos controles saudáveis]. A concentração de íons na saliva foi analisada pelo equipamento ABL 835 da Radiometer® e a concentração de cloreto no suor e sódio no suor, respectivamente, por titulação manual utilizando o método mercurimétrico de Schales & Schales e fotometria de chama. A análise estatística foi realizada pelo teste qui-quadrado, pelo teste de Mann-Whitney e pela correlação de Spearman. Alpha = 0,05. Resultados: Os pacientes com fibrose cística apresentaram maiores valores na concentração de cloreto no suor, concentração de sódio no suor, concentração de cloreto na saliva e concentração de sódio na saliva do que os indivíduos-controle saudáveis (valor de p < 0,001). A correlação entre as concentrações de cloreto na saliva e cloreto no suor mostrou Rho de Spearman (coeficiente de correlação) positivo = 0,475 (IC de 95% = 0,346 a 0,587). Além disso, a correlação entre concentração de sódio na saliva e concentração de sódio no suor mostrou Rho de Spearman positivo = 0,306 (IC de 95% = 0,158 a 0,440). Conclusões: A concentração de cloreto na saliva e a concentração de sódio na saliva são candidatas a ser usadas como diagnóstico de fibrose cística, principalmente em casos em que é difícil atingir a quantidade correta de suor, e/ou o exame da mutação RTFC é difícil e/ou o método de referência para o teste do suor não se encontra disponível ou não é de fácil acesso ao público em geral.

Abstract Objective: Cystic fibrosis diagnosis is dependent on the chloride ion concentration in the sweat test (≥ 60 mEq/mL - recognized as the gold standard indicator for cystic fibrosis diagnosis). Moreover, the salivary glands express the CFTR protein in the same manner as sweat glands. Given this context, the objective was to verify the correlation of saliva chloride concentration and sweat chloride concentration, and between saliva sodium concentration and sweat sodium concentration, in patients with cystic fibrosis and healthy control subjects, as a tool for cystic fibrosis diagnosis. Methods: There were 160 subjects enrolled: 57/160 (35.70%) patients with cystic fibrosis and two known CFTR mutations and 103/160 (64.40%) healthy controls subjects. Saliva ion concentration was analyzed by ABL 835 Radiometer® equipment and, sweat chloride concentration and sweat sodium concentration, respectively, by manual titration using the mercurimetric procedure of Schales & Schales and flame photometry. Statistical analysis was performed by the chi-squared test, the Mann -Whitney test, and Spearman's correlation. Alpha = 0.05. Results: Patients with cystic fibrosis showed higher values of sweat chloride concentration, sweat sodium concentration, saliva chloride concentration, and saliva sodium concentration than healthy controls subjects (p-value < 0.001). The correlation between saliva chloride concentration and sweat chloride concentration showed a positive Spearman's Rho (correlation coefficient) = 0.475 (95% CI = 0.346 to 0.587). Also, the correlation between saliva sodium concentration and sweat sodium concentration showed a positive Spearman's Rho = 0.306 (95% CI = 0.158 to 0.440). Conclusions: Saliva chloride concentration and saliva sodium concentration are candidates to be used in cystic fibrosis diagnosis, mainly in cases where it is difficult to achieve the correct sweat amount, and/or CFTR mutation screening is difficult, and/or reference methods for sweat test are unavailable to implement or are not easily accessible by the general population.

RESUMO Desde a publicação em 2015 das recomendações da Sociedade Brasileira de Hepatologia sobre a prevenção e tratamento de doenças hepáticas autoimunes, novos dados baseados em evidências científicas foram publicados na literatura, mudando o diagnóstico e tratamento da hepatite autoimune (HAI), colangite biliar primária (CBP), colangite esclerosante primária (CEP), síndromes de sobreposição de HAI, CBP e CEP e o manejo de complicações específicas além de outros tópicos relativos à HAI e doenças hepáticas colestáticas. Este manuscrito atualiza as recomendações anteriores de acordo com as melhores evidências disponíveis na literatura até o momento. O mesmo painel de experts que participou da primeira diretriz revisou todas as recomendações de acordo com os dados publicados na literatura e elaborou um manuscrito submetido subsequentemente à apreciação e revisão de todos os membros da Sociedade Brasileira de Hepatologia via homepage da sociedade. As recomendações finais atualizadas foram condensadas no presente documento.

ABSTRACT New data concerning the management of autoimmune liver diseases have emerged since the last single-topic meeting sponsored by the Brazilian Society of Hepatology to draw recommendations about the diagnosis and treatment of autoimmune hepatitis (AIH), primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC), overlap syndromes of AIH, PBC and PSC and specific complications and topics concerning AIH and cholestatic liver diseases. This manuscript updates those previous recommendations according to the best evidence available in the literature up to now. The same panel of experts that took part in the first consensus document reviewed all recommendations, which were subsequently scrutinized by all members of the Brazilian Society of Hepatology using a web-based approach. The new recommendations are presented herein.

Abstract Introduction: Ochroma pyramidale (Cav. ex Lam.) Urb. is a fast-growing native species of economic and ecological importance. It is currently the only species in the genus Ochroma. Objective: To determine the effect of seven pre-germination treatments applied on seeds of O. pyramidale in its typical variety and O. pyramidale var. bicolor (Rowlee) Brizicky in the Lacandon Jungle. Materials and methods: Seven germination treatments were evaluated: control, soaking in water at room temperature (Soaking12h and Soaking24h), immersion in boiling water at 100 °C (Boiling3s and Boiling10s) and immersion in boiling water and soaking in coconut water (Boiling3s + Soaking24h, and Boiling10s + Soaking24h). Coconut water was used as a natural source of cytokinins. Results and discussion: Germination of O. pyramidale in its typical variety (62 to 69 %) was statistically higher (P = 0.05) with treatments that included immersing the seeds in boiling water. In the case of O. pyramidale var. bicolor, the highest germination values were obtained by the Boiling3s (64 %) and Boiling3s + Soaking24h (59 %) treatments. The O. pyramidale var. bicolor seeds were susceptible to the boiling water immersion time, since germination was statistically greater (P = 0.05) at 3 s than at 10 s. Conclusions: Treatments that included immersion in boiling water had a greater effect on germination. Coconut water had no significant effect on the variable; therefore, the use of synthetic cytokinins is suggested in order to control the phytohormone dosage and thus verify its effect on germination.

Resumen Introducción: Ochroma pyramidale (Cav. ex Lam.) Urb. es una especie nativa de crecimiento rápido con importancia económica y ecológica. Actualmente, es la única especie del género Ochroma. Objetivo: Determinar el efecto de siete tratamientos pregerminativos aplicados en semillas de O. pyramidale en su variedad típica y O. pyramidale var. bicolor (Rowlee) Brizicky en la Selva Lacandona. Materiales y métodos: Se evaluaron siete tratamientos germinativos: testigo (control), remojo en agua a temperatura ambiente (Remojo12h y Remojo24h), inmersión en agua en ebullición a 100 °C (Ebullición3s y Ebullición10s) e inmersión en agua en ebullición y remojo en agua de coco (Ebullición3s + Remojo24h, y Ebullición10s + Remojo24h). El agua de coco se utilizó como fuente natural de citocininas. Resultados y discusión: La germinación de O. pyramidale en su variedad típica (62 a 69 %) fue estadísticamente mayor (P = 0.05) con los tratamientos que incluyeron inmersión de las semillas en agua en ebullición. En el caso de O. pyramidale var. bicolor, los valores más altos de germinación corresponden a los tratamientos Ebullición3s (64 %) y Ebullición3s + Remojo24h (59 %). Las semillas de O. pyramidale var. bicolor fueron susceptibles al tiempo de inmersión de agua en ebullición, ya que la germinación fue estadísticamente mayor (P = 0.05) a los 3 s que a los 10 s. Conclusión: Los tratamientos que incluyeron inmersión en agua en ebullición tuvieron mayor efecto sobre la germinación. El agua de coco no tuvo efecto significativo sobre la variable; por tanto, se sugiere el uso de citocininas sintéticas, con el fin de controlar la dosis de la fitohormona y así constatar su efecto en la germinación.