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au:AZEVEDO, JOAO L.
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1.
Molecular detection of Leishmania infantum in donkeys and mules under semiarid conditions in Brazil
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Limeira, Clécio Henrique
; Oliveira, Murilo Duarte de
; Araújo Júnior, João Pessoa
; Malossi, Camila Dantas
; Ullmann, Leila Sabrina
; Silva, Maria Luana Cristiny Rodrigues
; Azevedo, Sérgio Santos de
; Alves, Clebert José
.
Revista Brasileira de Parasitologia Veterinária
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Resumo A leishmaniose visceral é uma zoonose parasitária que afeta principalmente populações mais pobres e vulneráveis, e os cães domésticos são considerados as principais fontes de infecção para o vetor e, portanto, para os humanos. Porém diversos estudos têm pesquisado o papel de outros hospedeiros vertebrados no ciclo da doença. Neste contexto, objetivou-se realizar um levantamento da infecção por Leishmania infantum em asininos e muares, vivendo em região semiárida do Brasil. Foi utilizado sangue total de 72 equídeos (65 asininos e 7 muares) para a realização de diagnóstico molecular por meio da técnica de Reação em Cadeia de Polimerase em Tempo Real (qPCR). Um total de 25% das amostras (18/72) resultaram positivas na qPCR, porém não houve diferença significativa entre as espécies (asininos e muares), sexo (macho e fêmea) e situação de abandono dos animais (sim ou não). Asininos e muares, vivendo em condições semiáridas, apresentam alta frequência de infecção por L. infantum, sendo válido atribuir importância a essas espécies no ciclo epidemiológico da leishmaniose visceral, seja como um reservatório em potencial, seja apenas como uma fonte alimentar abundante para os vetores.
Abstract Visceral leishmaniasis is a parasitic zoonosis that mainly affects poorest and most vulnerable populations, and domestic dogs are considered to be the main source of infection to the vector and therefore humans. However, several studies have investigated the role of other vertebrate hosts in the disease cycle. In this context, the aim of the present study was to conduct a survey of Leishmania infantum infection in donkeys and mules living in a semiarid region of Brazil. Whole blood sampled from 72 equids (65 donkeys and 7 mules) was used to perform molecular diagnosis using the real-time polymerase chain reaction (qPCR) technique. A total of 25% of the samples (18/72) were positive through qPCR, but there were no significant differences between the species (donkeys or mules), sex (male or female) and abandonment situation of the animals (yes or no). Donkeys and mules living under semiarid conditions have high frequency of L. infantum infection. It is therefore worth assigning importance to these species in the epidemiological cycle of visceral leishmaniasis, either as potential reservoirs or just as an abundant food source for vectors.
https://doi.org/10.1590/s1984-29612021095
3 downloads
2.
Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research
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COELHO, CATARINA A.
; BORDELO, JOÃO P.
; CAMASSA, JOSÉ A.
; BARROS, VERA A.
; BABO, PEDRO S.
; GOMES, MANUELA E.
; REIS, RUI L.
; AZEVEDO, JORGE T. DE
; REQUICHA, JOÃO F.
; FAÍSCA, PEDRO
; CARVALHO, PEDRO P.
; VIEGAS, CARLOS A.
; DIAS, ISABEL R.
.
Abstract Osteoporosis is a metabolic disorder characterized by a loss of bone mass and structure and increasing the risk of fragility fractures, mostly among postmenopausal women. Sheep is a recognized large animal model for osteoporosis research. An experimental group of ewes (3-4 years old) was subjected to ovariectomy (OVX) and weekly glucocorticoid (GC) application for 24 weeks and compared with a sham control group. Blood and bone marrow parameters were analyzed before and 24 weeks after OVX and GC administration. Osteopenia was confirmed through micro-computed tomography and histomorphometric analysis of L4 vertebra in the study end. A statistically significant increase was observed in mean corpuscular volume, mean cell hemoglobin and monocytes and a decrease in red blood count and eosinophils (p<0.05). Alkaline phosphatase (ALP), gamma-glutamyl transpeptidase, magnesium and α1-globulin increased, and creatinine, albumin, sodium and estradiol decreased (p<0.05). A slight decrease of bone formation markers (bone ALP and osteocalcin) and an increase of bone resorption markers (C-terminal telopeptides of collagen type 1 and tartrate-resistant acid phosphatase) were observed, but without statistical significance. This study aims to contribute to better knowledge of sheep as a model for osteoporosis research and the consequences that a performed induction protocol may impose on organic metabolism.
https://doi.org/10.1590/0001-3765202020200435
677 downloads
3.
Leptospira interrogans infection in tegu lizard (Tupinambis merianae), Brazil
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Fernandes, Juciê Jales
; Pinheiro, Tiago Junior
; Costa, Diego Figueiredo
; Araújo Júnior, João Pessoa
; Malossi, Camila Dantas
; Ullmann, Leila Sabrina
; Silva, Maria Luana Cristiny Rodrigues
; Azevedo, Sérgio Santos
; Alves, Clebert José
; Higino, Severino Silvano dos Santos
.
RESUMO: Foram utilizados 12 lagartos Teiús (Tupinambis merianae) do Nordeste do Brasil. Encontramos nove animais positivos (75%) para Leptospira sp. na PCR e seis (50%) soropositivos. O sequenciamento de DNA de Leptospira sp. revelou 99% de semelhança com L. interrogans. Os resultados indicam que esta espécie pode desempenhar um papel importante na transmissão da leptospirose humana.
ABSTRACT: We used 12 tegu lizards (Tupinambis merianae) from northeastern Brazil, and we reported nine (75%) Leptospira sp. PCR-positive animals and six (50%) seropositive. Leptospira sp. DNA sequencing revealed 99% similarity with L. interrogans. Our findings indicated that this species may play a role in the transmission of human leptospirosis.
https://doi.org/10.1590/0103-8478cr20200424
463 downloads
4.
Physiological responses of lima bean subjected to salt and water stresses
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Pereira Filho, João V.
; Viana, Thales V. de A.
; Sousa, Geocleber G. de
; Chagas, Keivia L.
; Azevedo, Benito M. de
; Pereira, Carmem C. M. de S.
.
Revista Brasileira de Engenharia Agrícola e Ambiental
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RESUMO Sob condições de déficit hídrico e/ou estresse salino as culturas sofrem diversos distúrbios fisiológicos, que levam à redução no crescimento e, consequentemente, decréscimos em seus rendimentos agronômicos. Neste sentido, o objetivo do trabalho foi avaliar as respostas fisiológicas da cultura da fava, cultivar Espírito Santo, sob diferentes salinidades da água de irrigação associadas a dois regimes hídricos. O experimento foi conduzido na área experimental da Universidade Federal do Ceará, no período de setembro a novembro de 2017, sendo realizado em vasos a pleno sol, sob um delineamento estatístico inteiramente casualizado em parcelas subdivididas, onde as salinidades da água de irrigação (1,1; 2,1; 3,1; 4,1 e 5,1 dS m-1) foram consideradas como as parcelas, enquanto os regimes hídricos de 50 e 100% da evapotranspiração de referência - ETo, as subparcelas. Aos 45 dias após a semeadura avaliaram-se as trocas gasosas foliares e os índices relativos de clorofila total (índice SPAD). De maneira geral, o aumento da concentração de sais na água de irrigação provoca reduções nas respostas fisiológicas do feijão fava. Sob um regime hídrico de 50% da ETo, a eficiência instantânea e intrínseca do uso da água é maior.
ABSTRACT Under water deficit and/or salt stress conditions, crops suffer from several physiological disturbances, which lead to the reduction in growth and, consequently, decreases in their yields. Thus, the objective of this study was to evaluate the physiological responses of lima bean, cultivar Espírito Santo, under different irrigation water salinity associated to two water regimes. The experiment was carried out in the experimental area of the Universidade Federal do Ceará, Brazil, from September to November 2017, and it was carried out in pots in full sun, under a completely randomized design in split plots, where water salinities (1.1, 2.1, 3.1, 4.1 and 5.1 dS m-1) were considered as the plots, and the treatments of water regime (50 and 100% of the reference evapotranspiration - ETo) were considered as the subplots. At 45 days after sowing, leaf gas exchanges and relative index of total chlorophyll (SPAD index) were evaluated. In general, the increase of salt concentration in irrigation water causes reductions in the physiological responses of lima beans. Under a water regime of 50% ETo, the instantaneous and intrinsic water use efficiency is higher.
https://doi.org/10.1590/1807-1929/agriambi.v23n12p959-965
1304 downloads
5.
ENZYMATIC AND ANTAGONIST ACTIVITY OF ENDOPHYTIC FUNGI FROM Sapindus saponaria L. (SAPINDACEAE)
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SANTOS, Caroline Menicoze dos
; RIBEIRO, Amanda da Silva
; GARCIA, Adriana
; POLLI, Andressa Domingos
; POLONIO, Julio Cesar
; AZEVEDO, João Lúcio
; PAMPHILE, João Alencar
.
RESUMEN Los microorganismos endofíticos tienen gran interés biotecnológico, con características aplicables a diferentes áreas y potencialmente útiles en la agricultura. El presente estudio determinó el potencial biotecnológico de los hongos endofíticos, aislados de las hojas de Sapindus saponaria, en el control de hongos fitopatógenos y evaluación de su producción de enzimática. La taxonomía molecular fue realizada por la secuencia de la región ITS1-5.8S-ITS2 del ADN ribosomal, identificando los géneros Phomopsis, Sordariomycetes, Diaporthe y Colletotrichum. El antagonismo in vitro contra fitopatógenos mostró mejores resultados contra Fusarium solani y proporcionó índices de inhibición de entre el 41,8 % y el 67,5 %. El linaje endofítico SS81 (Diaporthe citri) presentó el mayor índice de antagonismo contra los patógenos. Contra Glomerella sp. y Moniliophthora perniciosa, las tasas de inhibición variaron entre el 18,7 % y el 57,4 % y entre el 38,3 % y el 64,8 %, respectivamente. El ensayo enzimático reveló que el linaje SS65 (Diaporthe sp.) produjo 1,16 UI μmol / min de amilasa; el linaje SS77 (Diaporthe sp.) produjo 2,74 UI μmol / min de pectinasa; y el linaje SS08 (Diaporthe sp.) produjo 1,51 UI μmol / min de celulasa. Así, el presente estudio evidencia la importancia de los endófitos aislados con propiedades fitoprotectoras como alternativas para el control biológico y como fuentes naturales de productos con interés biotecnológico.
ABSTRACT Endophyte microorganisms have great biotechnological interest, with features applicable to different areas and are potentially useful in agriculture. The current study determines the biotechnological potential of endophytic fungi, isolated from leaves of Sapindus saponaria, to control phytopathogenic fungi and evaluate their enzyme production. Molecular taxonomy was performed by sequencing of the ITS1-5.8S-ITS2 ribosomal DNA region, identifying the genera Phomopsis, Sordariomycetes, Diaporthe, and Colletotrichum. In vitro antagonism against phytopathogens showed better results against Fusarium solani and provided inhibition indices between 41.8 % and 67.5 %. The endophytic strain SS81 (Diaporthe citri) presented the highest antagonism index against the pathogen. Against Glomerella sp. and Moniliophthora perniciosa, inhibition rates ranged between 18.7 % and 57.4 % and between 38.3 % and 64.8 %, respectively. Enzyme assays revealed that strain SS65 (Diaporthe sp.) produced 1.16 UI μmol/min of amylase; strain SS77 (Diaporthe sp.) produced 2.74 UI μmol/min of pectinase, and strain SS08 (Diaporthe sp.) produced 1.51 UI μmol/min of cellulase. Thus, the current study shows evidence the importance of isolated endophytes with phytoprotective properties of plants with medicinal properties as alternatives for biological control and natural sources of products with biotechnological interest.
https://doi.org/10.15446/abc.v24n2.74717
988 downloads
6.
The potential of compounds isolated from Xylaria spp. as antifungal agents against anthracnose
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Elias, Luciana M.
; Fortkamp, Diana
; Sartori, Sérgio B.
; Ferreira, Marília C.
; Gomes, Luiz H.
; Azevedo, João L.
; Montoya, Quimi V.
; Rodrigues, André
; Ferreira, Antonio G.
; Lira, Simone P.
.
ABSTRACT Anthracnose is a crop disease usually caused by fungi in the genus Colletotrichum or Gloeosporium. These are considered one of the main pathogens, causing significant economic losses, such as in peppers and guarana. The current forms of control include the use of resistant cultivars, sanitary pruning and fungicides. However, even with the use of some methods of controlling these cultures, the crops are not free of anthracnose. Additionally, excessive application of fungicides increases the resistance of pathogens to agrochemicals and cause harm to human health and the environment. In order to find natural antifungal agents against guarana anthracnose, endophytic fungi were isolated from Amazon guarana. The compounds piliformic acid and cytochalasin D were isolated by chromatographic techniques from two Xylaria spp., guided by assays with Colletotrichum gloeosporioides. The isolated compounds were identified by spectrometric techniques, as NMR and mass spectrometry. This is the first report that piliformic acid and cytochalasin D have antifungal activity against C. gloeosporioides with MIC 2.92 and 2.46 µmol mL-1 respectively. Captan and difenoconazole were included as positive controls (MIC 16.63 and 0.02 µmol mL-1, respectively). Thus, Xylaria species presented a biotechnological potential and production of different active compounds which might be promising against anthracnose disease.
https://doi.org/10.1016/j.bjm.2018.03.003
1176 downloads
7.
ISOLATION OF CYTOTOXIC NEOLIGNANS FROM Saururus cernuus L. (SAURURACEAE) USING IONIC LIQUID IN THE MICROWAVE ASSISTED EXTRACTION (MAE)
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Brito, Juliana R.
; Camilo, Fernanda F.
; Figueiredo, Carlos R.
; Azevedo, Ricardo A.
; Romoff, Paulete
; Buturi, Fátima O. S.
; Fávero, Oriana A.
; Lago, João Henrique G.
; Ferreira, Edgard A.
.
In the present work, dried leaves of Saururus cernuus (Saururaceae) were subjected to extraction using an ionic liquid (1-butyl-3-methylimidazolium bromide - BMImBr) in the microwave assisted extraction (MAE). The obtained extract was partitioned using n-hexane and cytotoxicity activity of this organic phase against murine melanoma cell line (B16F10-Nex2) was evaluated in vitro. Since this extract displayed activity (100% of cell death at 200 µg mL-1) it was subjected to a bioactivity-guided fractionation to afford four related neolignans: threo-austrobailignan-5 (1), threo-austrobailignan-6 (2), threo-dihydroguaiaretic acid (3) and saucernetin (4). Their chemical structures were established based on NMR and MS spectral analysis. Among the isolated neolignans, compound 2 exhibited the highest cytotoxic activity against HeLa (human cervical melanoma) cells with IC50 of 28.3 ± 3.9 µg mL-1 (86 ± 12 µmol L-1). Furthermore, compounds 2 and 3 exhibited the highest cytotoxic activity against A2058 (human melanoma) cells with IC50 of 44.3 ± 4.2 µg mL-1 (135 ± 13 µmol L-1) and 41.5 ± 7.5 µg mL-1 (126 ± 23 µmol L-1), respectively, similar to positive control cisplatin (IC50 = 43.2 ± 3.2 µg mL-1 or 144 ± 11 µmol L-1). Otherwise, compound 4 was inactive (IC50 > 100 µg mL-1 or > 300 µmol L-1). The obtained results provide important data for the selection of bioactive neolignans with promising cytotoxic potential using a simple and fast method employing a green solvent as 1-butyl-3-methylimidazolium bromide (BMImBr).
https://doi.org/10.21577/0100-4042.20170246
490 downloads
8.
Lectotypes for species of Passiflora L. (Passifloraceae) described by João Barbosa Rodrigues
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ABSTRACT During taxonomic review of the Brazilian species of the Passiflora subgenus Astrophea and P. subg Decaloba, six names published by the Brazilian botanist João Barbosa Rodrigues were found in need of discussion: Passiflora alliacea, P. amalocarpa, P. cabedelensis, P. hexagonocarpa, P. hydrophila and Tacsonia coccinea. The original illustrations are here designated as lectotypes for P. alliacea, P. amalocarpa, P. cabedelensis (a synonym of P. amalocarpa), P. hexagonocarpa, P. hydrophila (a synonym of P. costata) and Tacsonia coccinea (a synonym of P. spinosa).
https://doi.org/10.1590/0102-33062016abb0330
1333 downloads
9.
TRIAGEM METABÓLICA POR PKS E NRPS EM ACTINOBACTÉRIAS ENDOFÍTICAS DE Citrus reticulata
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Cruz, Pedro L. R. da
; Giarola, Leila R.
; Moraes, Suellen da Silva
; Silva, Déborah Ellen S. G. da
; Marcon, Joelma
; Azevedo, João L.
; Araújo, Welington L.
; Oliveira,, Luciana G. de
.
Polyketides and non-ribosomal peptides are natural products widely found in bacteria, fungi and plants. The biological activities associated with these metabolites have attracted special attention in biopharmaceutical studies. Polyketide synthases act similarly to fatty acids synthetases and the whole multi-enzymatic set coordinating precursor and extending unit selection and reduction levels during chain growth. Acting in a similarly orchestrated model, non-ribosomal peptide synthetases biosynthesize NRPs. PKSs-I and NRPSs enzymatic modules and domains are collinearly organized with the parent gene sequence. This arrangement allows the use of degenerated PCR primers to amplify targeted regions in the genes corresponding to specific enzymatic domains such as ketosynthases and acyltransferases in PKSs and adenilation domains in NRPSs. Careful analysis of these short regions allows the classifying of a set of organisms according to their potential to biosynthesize PKs and NRPs. In this work, the biosynthetic potential of a set of 13 endophytic actinobacteria from Citrus reticulata for producing PKs and NRP metabolites was evaluated. The biosynthetic profile was compared to antimicrobial activity. Based on the inhibition promoted, 4 strains were considered for cluster analysis. A PKS/NRPS phylogeny was generated in order to classify some of the representative sequences throughout comparison with homologous genes. Using this approach, a molecular fingerprint was generated to help guide future studies on the most promising strains.
https://doi.org/10.5935/0100-4042.20150010
2525 downloads
10.
Growing knowledge: an overview of Seed Plant diversity in Brazil
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Zappi, Daniela C.
; Filardi, Fabiana L. Ranzato
; Leitman, Paula
; Souza, Vinícius C.
; Walter, Bruno M.T.
; Pirani, José R.
; Morim, Marli P.
; Queiroz, Luciano P.
; Cavalcanti, Taciana B.
; Mansano, Vidal F.
; Forzza, Rafaela C.
; Abreu, Maria C.
; Acevedo-Rodríguez, Pedro
; Agra, Maria F.
; Almeida Jr., Eduardo B.
; Almeida, Gracineide S.S.
; Almeida, Rafael F.
; Alves, Flávio M.
; Alves, Marccus
; Alves-Araujo, Anderson
; Amaral, Maria C.E.
; Amorim, André M.
; Amorim, Bruno
; Andrade, Ivanilza M.
; Andreata, Regina H.P.
; Andrino, Caroline O.
; Anunciação, Elisete A.
; Aona, Lidyanne Y.S.
; Aranguren, Yani
; Aranha Filho, João L.M.
; Araújo, Andrea O.
; Araújo, Ariclenes A.M.
; Araújo, Diogo
; Arbo, María M.
; Assis, Leandro
; Assis, Marta C.
; Assunção, Vivian A.
; Athiê-Souza, Sarah M.
; Azevedo, Cecilia O.
; Baitello, João B.
; Barberena, Felipe F.V.A.
; Barbosa, Maria R.V.
; Barros, Fábio
; Barros, Lucas A.V.
; Barros, Michel J.F.
; Baumgratz, José F.A.
; Bernacci, Luis C.
; Berry, Paul E.
; Bigio, Narcísio C.
; Biral, Leonardo
; Bittrich, Volker
; Borges, Rafael A.X.
; Bortoluzzi, Roseli L.C.
; Bove, Cláudia P.
; Bovini, Massimo G.
; Braga, João M.A.
; Braz, Denise M.
; Bringel Jr., João B.A.
; Bruniera, Carla P.
; Buturi, Camila V.
; Cabral, Elza
; Cabral, Fernanda N.
; Caddah, Mayara K.
; Caires, Claudenir S.
; Calazans, Luana S.B.
; Calió, Maria F.
; Camargo, Rodrigo A.
; Campbell, Lisa
; Canto-Dorow, Thais S.
; Carauta, Jorge P.P.
; Cardiel, José M.
; Cardoso, Domingos B.O.S.
; Cardoso, Leandro J.T.
; Carneiro, Camila R.
; Carneiro, Cláudia E.
; Carneiro-Torres, Daniela S.
; Carrijo, Tatiana T.
; Caruzo, Maria B.R.
; Carvalho, Maria L.S.
; Carvalho-Silva, Micheline
; Castello, Ana C.D.
; Cavalheiro, Larissa
; Cervi, Armando C.
; Chacon, Roberta G.
; Chautems, Alain
; Chiavegatto, Berenice
; Chukr, Nádia S.
; Coelho, Alexa A.O.P.
; Coelho, Marcus A.N.
; Coelho, Rubens L.G.
; Cordeiro, Inês
; Cordula, Elizabeth
; Cornejo, Xavier
; Côrtes, Ana L.A.
; Costa, Andrea F.
; Costa, Fabiane N.
; Costa, Jorge A.S.
; Costa, Leila C.
; Costa-e-Silva, Maria B.
; Costa-Lima, James L.
; Cota, Maria R.C.
; Couto, Ricardo S.
; Daly, Douglas C.
; De Stefano, Rodrigo D.
; De Toni, Karen
; Dematteis, Massimiliano
; Dettke, Greta A.
; Di Maio, Fernando R.
; Dórea, Marcos C.
; Duarte, Marília C.
; Dutilh, Julie H.A.
; Dutra, Valquíria F.
; Echternacht, Lívia
; Eggers, Lilian
; Esteves, Gerleni
; Ezcurra, Cecilia
; Falcão Junior, Marcus J.A.
; Feres, Fabíola
; Fernandes, José M.
; Ferreira, D.M.C.
; Ferreira, Fabrício M.
; Ferreira, Gabriel E.
; Ferreira, Priscila P.A.
; Ferreira, Silvana C.
; Ferrucci, Maria S.
; Fiaschi, Pedro
; Filgueiras, Tarciso S.
; Firens, Marcela
; Flores, Andreia S.
; Forero, Enrique
; Forster, Wellington
; Fortuna-Perez, Ana P.
; Fortunato, Reneé H.
; Fraga, Cléudio N.
; França, Flávio
; Francener, Augusto
; Freitas, Joelcio
; Freitas, Maria F.
; Fritsch, Peter W.
; Furtado, Samyra G.
; Gaglioti, André L.
; Garcia, Flávia C.P.
; Germano Filho, Pedro
; Giacomin, Leandro
; Gil, André S.B.
; Giulietti, Ana M.
; A.P.Godoy, Silvana
; Goldenberg, Renato
; Gomes da Costa, Géssica A.
; Gomes, Mário
; Gomes-Klein, Vera L.
; Gonçalves, Eduardo Gomes
; Graham, Shirley
; Groppo, Milton
; Guedes, Juliana S.
; Guimarães, Leonardo R.S.
; Guimarães, Paulo J.F.
; Guimarães, Elsie F.
; Gutierrez, Raul
; Harley, Raymond
; Hassemer, Gustavo
; Hattori, Eric K.O.
; Hefler, Sonia M.
; Heiden, Gustavo
; Henderson, Andrew
; Hensold, Nancy
; Hiepko, Paul
; Holanda, Ana S.S.
; Iganci, João R.V.
; Imig, Daniela C.
; Indriunas, Alexandre
; Jacques, Eliane L.
; Jardim, Jomar G.
; Kamer, Hiltje M.
; Kameyama, Cíntia
; Kinoshita, Luiza S.
; Kirizawa, Mizué
; Klitgaard, Bente B.
; Koch, Ingrid
; Koschnitzke, Cristiana
; Krauss, Nathália P.
; Kriebel, Ricardo
; Kuntz, Juliana
; Larocca, João
; Leal, Eduardo S.
; Lewis, Gwilym P.
; Lima, Carla T.
; Lima, Haroldo C.
; Lima, Itamar B.
; Lima, Laíce F.G.
; Lima, Laura C.P.
; Lima, Leticia R.
; Lima, Luís F.P.
; Lima, Rita B.
; Lírio, Elton J.
; Liro, Renata M.
; Lleras, Eduardo
; Lobão, Adriana
; Loeuille, Benoit
; Lohmann, Lúcia G.
; Loiola, Maria I.B.
; Lombardi, Julio A.
; Longhi-Wagner, Hilda M.
; Lopes, Rosana C.
; Lorencini, Tiago S.
; Louzada, Rafael B.
; Lovo, Juliana
; Lozano, Eduardo D.
; Lucas, Eve
; Ludtke, Raquel
; Luz, Christian L.
; Maas, Paul
; Machado, Anderson F.P.
; Macias, Leila
; Maciel, Jefferson R.
; Magenta, Mara A.G.
; Mamede, Maria C.H.
; Manoel, Evelin A.
; Marchioretto, Maria S.
; Marques, Juliana S.
; Marquete, Nilda
; Marquete, Ronaldo
; Martinelli, Gustavo
; Martins da Silva, Regina C.V.
; Martins, Ângela B.
; Martins, Erika R.
; Martins, Márcio L.L.
; Martins, Milena V.
; Martins, Renata C.
; Matias, Ligia Q.
; Maya-L., Carlos A.
; Mayo, Simon
; Mazine, Fiorella
; Medeiros, Debora
; Medeiros, Erika S.
; Medeiros, Herison
; Medeiros, João D.
; Meireles, José E.
; Mello-Silva, Renato
; Melo, Aline
; Melo, André L.
; Melo, Efigênia
; Melo, José I.M.
; Menezes, Cristine G.
; Menini Neto, Luiz
; Mentz, Lilian A.
; Mezzonato, A.C.
; Michelangeli, Fabián A.
; Milward-de-Azevedo, Michaele A.
; Miotto, Silvia T.S.
; Miranda, Vitor F.O.
; Mondin, Cláudio A.
; Monge, Marcelo
; Monteiro, Daniele
; Monteiro, Raquel F.
; Moraes, Marta D.
; Moraes, Pedro L.R.
; Mori, Scott A.
; Mota, Aline C.
; Mota, Nara F.O.
; Moura, Tania M.
; Mulgura, Maria
; Nakajima, Jimi N.
; Nardy, Camila
; Nascimento Júnior, José E.
; Noblick, Larry
; Nunes, Teonildes S.
; O'Leary, Nataly
; Oliveira, Arline S.
; Oliveira, Caetano T.
; Oliveira, Juliana A.
; Oliveira, Luciana S.D.
; Oliveira, Maria L.A.A.
; Oliveira, Regina C.
; Oliveira, Renata S.
; Oliveira, Reyjane P.
; Paixão-Souza, Bruno
; Parra, Lara R.
; Pasini, Eduardo
; Pastore, José F.B.
; Pastore, Mayara
; Paula-Souza, Juliana
; Pederneiras, Leandro C.
; Peixoto, Ariane L.
; Pelissari, Gisela
; Pellegrini, Marco O.O.
; Pennington, Toby
; Perdiz, Ricardo O.
; Pereira, Anna C.M.
; Pereira, Maria S.
; Pereira, Rodrigo A.S.
; Pessoa, Clenia
; Pessoa, Edlley M.
; Pessoa, Maria C.R.
; Pinto, Luiz J.S.
; Pinto, Rafael B.
; Pontes, Tiago A.
; Prance, Ghillean T.
; Proença, Carolyn
; Profice, Sheila R.
; Pscheidt, Allan C.
; Queiroz, George A.
; Queiroz, Rubens T.
; Quinet, Alexandre
; Rainer, Heimo
; Ramos, Eliana
; Rando, Juliana G.
; Rapini, Alessandro
; Reginato, Marcelo
; Reis, Ilka P.
; Reis, Priscila A.
; Ribeiro, André R.O.
; Ribeiro, José E.L.S.
; Riina, Ricarda
; Ritter, Mara R.
; Rivadavia, Fernando
; Rocha, Antônio E.S.
; Rocha, Maria J.R.
; Rodrigues, Izabella M.C.
; Rodrigues, Karina F.
; Rodrigues, Rodrigo S.
; Rodrigues, Rodrigo S.
; Rodrigues, Vinícius T.
; Rodrigues, William
; Romaniuc Neto, Sérgio
; Romão, Gerson O.
; Romero, Rosana
; Roque, Nádia
; Rosa, Patrícia
; Rossi, Lúcia
; Sá, Cyl F.C.
; Saavedra, Mariana M.
; Saka, Mariana
; Sakuragui, Cássia M.
; Salas, Roberto M.
; Sales, Margareth F.
; Salimena, Fatima R.G.
; Sampaio, Daniela
; Sancho, Gisela
; Sano, Paulo T.
; Santos, Alessandra
; Santos, Élide P.
; Santos, Juliana S.
; Santos, Marianna R.
; Santos-Gonçalves, Ana P.
; Santos-Silva, Fernanda
; São-Mateus, Wallace
; Saraiva, Deisy P.
; Saridakis, Dennis P.
; Sartori, Ângela L.B.
; Scalon, Viviane R.
; Schneider, Ângelo
; Sebastiani, Renata
; Secco, Ricardo S.
; Senna, Luisa
; Senna-Valle, Luci
; Shirasuna, Regina T.
; Silva Filho, Pedro J.S.
; Silva, Anádria S.
; Silva, Christian
; Silva, Genilson A.R.
; Silva, Gisele O.
; Silva, Márcia C.R.
; Silva, Marcos J.
; Silva, Marcos J.
; Silva, Otávio L.M.
; Silva, Rafaela A.P.
; Silva, Saura R.
; Silva, Tania R.S.
; Silva-Gonçalves, Kelly C.
; Silva-Luz, Cíntia L.
; Simão-Bianchini, Rosângela
; Simões, André O.
; Simpson, Beryl
; Siniscalchi, Carolina M.
; Siqueira Filho, José A.
; Siqueira, Carlos E.
; Siqueira, Josafá C.
; Smith, Nathan P.
; Snak, Cristiane
; Soares Neto, Raimundo L.
; Soares, Kelen P.
; Soares, Marcos V.B.
; Soares, Maria L.
; Soares, Polyana N.
; Sobral, Marcos
; Sodré, Rodolfo C.
; Somner, Genise V.
; Sothers, Cynthia A.
; Sousa, Danilo J.L.
; Souza, Elnatan B.
; Souza, Élvia R.
; Souza, Marcelo
; Souza, Maria L.D.R.
; Souza-Buturi, Fátima O.
; Spina, Andréa P.
; Stapf, María N.S.
; Stefano, Marina V.
; Stehmann, João R.
; Steinmann, Victor
; Takeuchi, Cátia
; Taylor, Charlotte M.
; Taylor, Nigel P.
; Teles, Aristônio M.
; Temponi, Lívia G.
; Terra-Araujo, Mário H.
; Thode, Veronica
; Thomas, W.Wayt
; Tissot-Squalli, Mara L.
; Torke, Benjamin M.
; Torres, Roseli B.
; Tozzi, Ana M.G.A.
; Trad, Rafaela J.
; Trevisan, Rafael
; Trovó, Marcelo
; Valls, José F.M.
; Vaz, Angela M.S.F.
; Versieux, Leonardo
; Viana, Pedro L.
; Vianna Filho, Marcelo D.M.
; Vieira, Ana O.S.
; Vieira, Diego D.
; Vignoli-Silva, Márcia
; Vilar, Thaisa
; Vinhos, Franklin
; Wallnöfer, Bruno
; Wanderley, Maria G.L.
; Wasshausen, Dieter
; Watanabe, Maurício T.C.
; Weigend, Maximilian
; Welker, Cassiano A.D.
; Woodgyer, Elizabeth
; Xifreda, Cecilia C.
; Yamamoto, Kikyo
; Zanin, Ana
; Zenni, Rafael D.
; Zickel, Carmem S
.
Resumo Um levantamento atualizado das plantas com sementes e análises relevantes acerca desta biodiversidade são apresentados. Este trabalho se iniciou em 2010 com a publicação do Catálogo de Plantas e Fungos e, desde então vem sendo atualizado por mais de 430 especialistas trabalhando online. O Brasil abriga atualmente 32.086 espécies nativas de Angiospermas e 23 espécies nativas de Gimnospermas e estes novos dados mostram um aumento de 3% da riqueza em relação a 2010. A Amazônia é o Domínio Fitogeográfico com o maior número de espécies de Gimnospermas, enquanto que a Floresta Atlântica possui a maior riqueza de Angiospermas. Houve um crescimento considerável no número de espécies e nas taxas de endemismo para a maioria dos Domínios (Caatinga, Cerrado, Floresta Atlântica, Pampa e Pantanal), com exceção da Amazônia que apresentou uma diminuição de 2,5% de endemicidade. Entretanto, a maior parte das plantas com sementes que ocorrem no Brasil (57,4%) é endêmica deste território. A proporção de formas de vida varia de acordo com os diferentes Domínios: árvores são mais expressivas na Amazônia e Floresta Atlântica do que nos outros biomas, ervas são dominantes no Pampa e as lianas apresentam riqueza expressiva na Amazônia, Floresta Atlântica e Pantanal. Este trabalho não só quantifica a biodiversidade brasileira, mas também indica as lacunas de conhecimento e o desafio a ser enfrentado para a conservação desta flora.
Abstract An updated inventory of Brazilian seed plants is presented and offers important insights into the country's biodiversity. This work started in 2010, with the publication of the Plants and Fungi Catalogue, and has been updated since by more than 430 specialists working online. Brazil is home to 32,086 native Angiosperms and 23 native Gymnosperms, showing an increase of 3% in its species richness in relation to 2010. The Amazon Rainforest is the richest Brazilian biome for Gymnosperms, while the Atlantic Rainforest is the richest one for Angiosperms. There was a considerable increment in the number of species and endemism rates for biomes, except for the Amazon that showed a decrease of 2.5% of recorded endemics. However, well over half of Brazillian seed plant species (57.4%) is endemic to this territory. The proportion of life-forms varies among different biomes: trees are more expressive in the Amazon and Atlantic Rainforest biomes while herbs predominate in the Pampa, and lianas are more expressive in the Amazon, Atlantic Rainforest, and Pantanal. This compilation serves not only to quantify Brazilian biodiversity, but also to highlight areas where there information is lacking and to provide a framework for the challenge faced in conserving Brazil's unique and diverse flora.
https://doi.org/10.1590/2175-7860201566411
33340 downloads
11.
Diversidade e usos de plantas medicinais nos quintais da comunidade de São João da Várzea em Mossoró, RN
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RESUMO Nos quintais do nordeste brasileiro são mantidas tradicionalmente diversas espécies vegetais para o tratamento de doenças. O objetivo do presente trabalho foi realizar um levantamento das espécies medicinais em quintais na Comunidade de São João da Várzea, Mossoró, Rio Grande do Norte, Brasil. Participaram do estudo 22 informantes e foram aplicadas para a coleta de dados técnicas de observação direta e participante, turnê guiada, lista livre e entrevistas semiestruturadas. Verificou-se a presença de 57 espécies pertencentes a 37 famílias botânicas. As espécies com maior frequência de ocorrência foram goiabeira (Psidium guajavaL.) e coqueiro (Cocos nucifera L.), presentes em 59% e 55% dos quintais, respectivamente. Para estas espécies houve maior índice de concordância de uso (CUPc de 61,5% e 92,3%). Foram citadas 67 patologias e o maior número de plantas medicinais foi indicado para o tratamento de gripe, disenteria, inflamações e como cicatrizante de ferimentos. O principal modo de administração foi por via oral, na forma de decocção (58%) e as folhas foram as partes mais usadas (32,6%). Os quintais são sistemas que atuam como uma estratégia de uso mais sustentável dos recursos medicinais por conservarem também as espécies nativas. As espécies medicinais mais frequentes nos quintais são arvores frutífero-alimentícias importantes para a renda familiar. A gripe e desinteria são os principais agravos de saúde tratados com espécies medicinais pela comunidade. Ziziphus joazeiro Mart., Citrus X limonia (L.) Osbeck, Punica granatum L., Cocos nucifera L. e Psidium guajava L. são espécies que merecem maiores estudos farmacológicos.
ABSTRACT Traditionally, at homegardens in the northeastern of Brazil, several plant species are employed for the treatment of diseases. The aim of this study was to research the species of medicinal use in homegardens of São João da Varzea, Mossoró, Rio Grande do Norte, Brazil. 22 homegardens were studied through the techniques of direct and participant observations , guided tours, free list and semi structured interviews. We verified the presence of 57 botanical species belonging to 37 families. The two species with the highest frequency of occurrence were the guava (Psidium guajava L.) and the coconut (Cocos nucifera L.), present in 59% and 55% of the homegardens, respectively. For these species there was more agreement of use (CUPc 61.5% and 92.3%). 67 diseases, including influenza and dysentery were the most common ones mentioned during the interviews. The main mode of administration was orally, in the form of decoction (58%), and the leaves were the most employed component (32.6%). Homegardens are systems that act as a more sustainable use of medicinal resources strategy since they conserve native species. The most common medicinal plants in the homegardens are the fruitful-food trees, important to the family income. The flu and the dysentery are the main health problems treated with medicinal plants by the community. The Ziziphus joazeiro Mart., Citrus X limonia (L.) Osbeck, Punica granatum L., Cocos nucifera L. and Psidium guajava L. are species that deserve further pharmacological studies.
https://doi.org/10.1590/1983-084X/14_080
4476 downloads
12.
II Diretriz de Ressonância Magnética e Tomografia Computadorizada Cardiovascular da Sociedade Brasileira de Cardiologia e do Colégio Brasileiro de Radiologia
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Sara, Leonardo
; Szarf, Gilberto
; Tachibana, Adriano
; Shiozaki, Afonso Akio
; Villa, Alexandre Volney
; Oliveira, Amarino Carvalho de
; Albuquerque, Andrei Skromov de
; Rochitte, Carlos Eduardo
; Nomura, César Higa
; Azevedo, Clerio Francisco
; Jasinowodolinski, Dany
; Tassi, Eduardo Marinho
; Medeiros, Fabio de Morais
; Kay, Fernando Uliana
; Junqueira, Flávia Pegado
; Azevedo, Guilherme S. A.
; Monte, Guilherme Urpia
; Pinto, Ibraim Masciarelli Francisco
; Gottlieb, Ilan
; Andrade, Joalbo
; Lima, João A. C.
; Parga Filho, José Rodrigues
; Kelendjian, Juliana
; Fernandes, Juliano Lara
; Iquizli, Leonardo
; Correia, Luis C. L.
; Quaglia, Luiz Augusto
; Gonçalves, Luiz Flavio Galvão
; Ávila, Luiz Francisco
; Zapparoli, Marcello
; Hadlich, Marcelo
; Nacif, Marcelo Souto
; Barbosa, Márcia de Melo
; Minami, Márcio Hiroshi
; Bittencourt, Marcio Sommer
; Siqueira, Maria Helena Albernaz
; Silva, Marly Conceição
; Lopes, Marly Maria Uellendahl
; Marques, Mateus Diniz
; Vieira, Mônica La Rocca
; Coellho Filho, Otávio Rizzi
; Schvartzman, Paulo R.
; Santos, Raul D.
; Cury, Ricardo C.
; Loureiro, Ricardo
; Cury, Roberto Caldeira
; Sasdelli Neto, Roberto
; Macedo, Robson
; Cerci, Rodrigo Julio
; Faria Filho, Rui Alberto de
; Cardoso, Sávio
; Naves, Thiago
; Magalhães, Tiago Augusto
; Senra, Tiago
; Burgos, Ursula Maria Moreira Costa
; Moreira, Valéria de Melo
; Ishikawa, Walther Yoshiharu
.
https://doi.org/10.5935/abc.2014S006
67586 downloads
13.
Survey of potential sharpshooter and spittlebug vectors of Xylella fastidiosa to grapevines at the São Francisco River Valley, Brazil
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Ringenberg, Rudiney
; Lopes, João Roberto Spotti
; Müller, Cristiane
; Azevedo-Filho, Wilson Sampaio de
; Paranhos, Beatriz Aguiar Jordão
; Botton, Marcos
.
Survey of potential sharpshooter and spittlebug vectors of Xylella fastidiosa to grapevines at the São Francisco River Valley, Brazil. Pierce's disease of grapevines, caused by Xylella fastidiosa, is a serious problem in some regions of North America, not yet reported in Brazil. In this study, a survey of potential sharpshooter (Hemiptera, Cicadellidae, Cicadellinae) and spittlebug (Hemiptera, Cercopidae) vectors of X. fastidiosa was conducted in vineyards at the São Francisco River Valley, a major grape growing region in Brazil. Four vineyards of Vitis vinifera L. were sampled fortnightly from June/2005 to June/2007, using yellow sticky cards, each placed at two different heights (45 cm aboveground and 45 cm above the crop canopy) in 10 sampling localities. A total of 4,095 specimens of sharpshooters were collected, nearly all from 3 Proconiini species, Homalodisca spottii Takiya, Cavichioli & McKamey, 2006 (96.8% of the specimens), Tapajosa fulvopunctata (Signoret, 1854) (3.1%), and Tretogonia cribrata Melichar, 1926 (1 specimen). Hortensia similis (Walker, 1851) (2 specimens) was the only Cicadellini species. Only 1 cercopid specimen, belonging to Aeneolamia colon (Germar, 1821), was trapped. Even though they are not considered potential Xylella vectors, 2 Gyponini leafhoppers were collected: Curtara samera DeLong & Freytag, 1972 (11 specimens) and Curtara inflata DeLong & Freytag, 1976 (1 specimen). Homalodisca spottii was observed feeding and mating on green branches of grapevines, in addition to egg masses. Because of its prevalence on the crop canopy, occurrence throughout the year (with peaks from February to August), and ability to colonize grapevines, H. spottii could be an important vector if a X. fastidiosa strain pathogenic to grapevines becomes introduced at the São Francisco River Valley.
https://doi.org/10.1590/S0085-56262014000200013
3936 downloads
14.
ASPECTS OF THE ECOLOGY OF PHLEBOTOMINES (Diptera: Psychodidae: Phlebotominae) IN AN AREA OF CUTANEOUS LEISHMANIASIS OCCURRENCE, MUNICIPALITY OF ANGRA DOS REIS, COAST OF RIO DE JANEIRO STATE, BRAZIL
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Aguiar, Gustavo Marins de
; Azevedo, Alfredo Carlos Rodrigues de
; Medeiros, Wagner Muniz de
; Alves, João Ricardo Carreira
; Rendeiro, Vanessa
.
Revista do Instituto de Medicina Tropical de São Paulo
- Journal Metrics
Durante dois anos completos foram feitas capturas de flebotomíneos em área de leishmaniose tegumentar no município de Angra dos Reis. Utilizou-se tubo de sucção manual, para as capturas dos flebotomíneos pousados nas paredes da casa, além de armadilhas luminosas, no domicílio, peridomicílio e na mata. Foram obtidos 14.170 exemplares, de treze espécies, duas do gênero Brumptomyia França & Parrot 1921 e onze do gênero Lutzomyia França 1924. L. intermedia teve supremacia no peridomicílio e no domicílio, com pouca presença na mata, o mesmo ocorreu com L. migonei, comprovando a adaptação dessas espécies ao ambiente humano. L. fischeri aparece com característica eclética quanto ao local, mostrando-se proporcionalmente mais endófila. L. intermedia e L. migonei foram mais numerosas no peridomicílio, durante todos os meses do ano, enquanto L. fischeri, excetuando os meses de março, abril, maio e setembro, foi mais numerosa no domicílio. Pela prevalência, comprovada antropofilia e por ter sido encontrada infectada naturalmente por Leishmania(Viannia) braziliensis, L. intermedia pode ser incriminada como o principal vetor desse agente da leishmaniose tegumentar na área de estudo, sobretudo no ambiente peridomiciliar. L. fischeri, pelas características apresentadas, pode ser um coadjuvante na veiculação do parasita.
Over a complete two-year period, phlebotomine specimens were caught in an area of cutaneous leishmaniasis occurrence in the municipality of Angra dos Reis. A manual suction tube was used to catch phlebotomines on house walls, and also light traps in domestic and peridomestic settings and in the forest. This yielded 14,170 specimens of 13 species: two in the genus Brumptomyia and eleven in the genus Lutzomyia. L. intermedia predominantly in domestic and peridomestic settings, with little presence in the forest, with the same trend being found in relation to L. migonei, thus proving that these species have adapted to the human environment. L. fischeri appeared to be eclectic regarding location, but was seen to be proportionally more endophilic. L. intermedia and L. migonei were more numerous in peridomestic settings, throughout the year, while L. fischeri was more numerous in domestic settings except in March, April, May and September. From the prevalence of L. intermedia, its proven anthropophily and findings of this species naturally infected with Leishmania(Viannia) braziliensis, it can be incriminated as the main vector for this agent of cutaneous leishmaniasis in the study area, especially in the peridomestic environment. L. fischeri may be a coadjuvant in carrying the parasite.
https://doi.org/10.1590/S0036-46652014000200010
2530 downloads
15.
Componentes de produção do girassol Embrapa 122-V2000 sob salinidade da água e adubação nitrogenada
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Centeno, Cruz R. M.
; Santos, João B. dos
; Xavier, Diego A.
; Azevedo, Carlos A. V. de
; Gheyi, Hans R.
.
RESUMO O trabalho foi realizado com objetivo de avaliar os componentes de produção da cultura do girassol, submetida à irrigação com águas salinizadas e adubação nitrogenada. O experimento foi conduzido em ambiente protegido pertencente a Universidade Federal de Campina Grande, durante o período de abril a julho de 2012, em delineamento inteiramente casualizado usando o arranjo fatorial 5 x 5, referente aos níveis de salinidade da água de irrigação, expressas pelas condutividades elétricas: 0,7; 1,7; 2,7; 3,7 e 4,7 dS m-1 (25 oC) e as doses de nitrogênio: 50; 75; 100; 125 e 150 mg kg-1, com três repetições, totalizando 75 unidades experimentais. Avaliou-se o número total de aquênios, produção de aquênios, porcentagem de aquênios viáveis, porcentagem de fitomassa de aquênios viáveis, fitomassa do capitulo, fitomassa de 1000 aquênios, fitomassa seca da parte aérea e da raiz. A salinidade da água de irrigação afetou a produção de aquênios, número total de aquênios, porcentagem de número de aquênios viáveis, porcentagem de fitomassa de aquênios viáveis, fitomassa do capitulo, fitomassa de 1000 aquênios, fitomassa seca da parte aérea e da raiz, independentemente da dose de nitrogênio utilizada. As plantas de girassol cv. EMBRAPA 122-V2000 foram afetadas de forma distinta, tanto pela salinidade da água de irrigação, quanto pela adubação nitrogenada, embora não tenha ocorrido efeito significativo da interação entre os fatores. A aplicação de doses crescentes de nitrogênio promoveu aumento linear na produção de aquênios, número total de aquênios, fitomassa do capitulo e fitomassa seca da raiz.
ABSTRACT The study was conducted to assess the variables of sunflower production, subjected to irrigation with saline water and nitrogen fertilization. The experiment was conducted in a greenhouse at the Federal University of Campina Grande, from April to July 2012 in a completely randomized design using the 5 x 5 factorial scheme, related to salinity levels of irrigation water expressed by electrical conductivity of 0.7, 1.7, 2.7, 3.7 and 4.7 dS m-1 at 25 oC and nitrogen levels: 50, 75, 100, 125 and 150 mg kg-1, with three replications, totaling 75 experimental units. The total number of achenes, production of achene, percentage of viable achenes, percentage of biomass of viable achenes, the chapter biomass, dry weight of 1000 seeds, dry weight of shoot and root were evaluated. The salinity of water affected the production of achenes, the total number of achenes, percentage of number of viable achenes, percentage of biomass of viable achenes, the biomass of chapter, biomass of 1000 seeds, dry weight of shoot and root, regardless of the dose of nitrogen used. The sunflower cv. EMBRAPA 122-V2000 was affected by salinity of water, as well as by nitrogen fertilization, though there was no significant effect of their interaction. The increasing rates of nitrogen caused a linear increase in achene production, total number of achenes, the biomass and dry biomass of chapter and root.
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