ABSTRACT Introduction: Recently, the obesity rate in Chinese universities has increased substantially and the indicators of physical fitness have declined. The physical quality of college students is an alarming problem that cannot be ignored. The results of structured work could establish a basis for colleges and universities to formulate scientific functional physical training programs. Objective: Analyze the role of functional physical training in teaching physical education to male college students in colleges and universities. Methods: 116 male volunteer students from 4 colleges and universities were selected and divided into control and experimental groups. The groups received three physical education classes lasting 90 minutes for eight weeks. The experimental group received an additional 30 minutes of functional physical training. Both groups were tested for vital capacity, seated forward bending, standing long jump, 50-meter run, pull-up, and 1000-meter test. The tests were compared and statistica50-meteryzed. Results: After 1000-meters of training, the experimental group was superior to the control group on all measures except sitting forward flexion. The results showed significant changes (P<0.01). The restorative effect of the experimental group was significantly enhanced. There was no significant difference in body weight, BMI, 50-meter run, jump, vital capacity, pull-up, and other indicators before and after control training (P>0.05). Conclusion: The 8 weeks of functional physical training promoted a significant effect on improving the physical health of college students. Functional physical training should be appropriately reinforced in university physical education classes to improve students' physical fitness. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMEN Introducción: Recientemente, la tasa de obesidad en las universidades chinas ha aumentado considerablemente y los indicadores de aptitud física han disminuido. La calidad física de los estudiantes universitarios es un problema alarmante que no se puede ignorar. Los resultados de un trabajo estructurado podrían sentar las bases para que los colegios y universidades formulen programas científicos de entrenamiento físico funcional. Objetivo: Analizar el papel del entrenamiento físico funcional en la enseñanza de la educación física de los estudiantes universitarios varones en los colegios y universidades. Métodos: Se seleccionaron 116 estudiantes voluntarios varones de 4 colegios y universidades, divididos en dos grupos: control y experimental. Los grupos recibieron tres clases de educación física de 90 minutos durante ocho semanas. El grupo experimental recibió 30 minutos adicionales de entrenamiento físico funcional. Ambos grupos fueron sometidos a pruebas de capacidad vital, flexión hacia delante sentada, salto de longitud de pie, carrera de 50 metros, dominadas y prueba de 1000 metros. Las pruebas se compararon y se analizaron estadísticamente. Resultados: Tras ocho semanas de entrenamiento, el grupo experimental fue superior al grupo de control en todas las medidas, excepto en la flexión hacia delante en posición sentada. Los resultados mostraron cambios significativos (P<0,01). El efecto restaurador del grupo experimental fue significativamente mayor. No hubo diferencias significativas en el peso corporal, el IMC, la carrera de 50 metros, el salto, la capacidad vital, la dominada y otros indicadores antes y después del entrenamiento de control (P>0,05). Conclusión: Las 8 semanas de entrenamiento físico funcional promovieron un efecto significativo en la mejora de la salud física de los estudiantes universitarios. El entrenamiento físico funcional debe reforzarse adecuadamente en las clases de educación física de la universidad para mejorar la aptitud física de los estudiantes. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

RESUMO Introdução: Recentemente, a taxa de obesidade nas universidades chinesas tem aumentado substancialmente e os indicadores de aptidão física têm diminuído. A qualidade física dos estudantes universitários é um problema alarmante que não pode ser ignorado. Os resultados de um trabalho estruturado poderiam estabelecer uma base para que faculdades e universidades possam formular programas científicos de treinamento físico funcional. Objetivo: Analisar o papel do treinamento físico funcional no ensino da educação física de estudantes universitários do sexo masculino em faculdades e universidades. Métodos: Foram selecionados 116 estudantes voluntários do sexo masculino em 4 faculdades e universidades, divididos em dois grupos: controle e experimental. Os grupos receberam três aulas de educação física com duração de 90 minutos durante oito semanas. O grupo experimental recebeu 30 minutos adicionais de treinamento físico funcional. Os dois grupos foram testados quanto à capacidade vital, flexão sentado para frente, salto em pé à distância, corrida de 50 metros, pull-up e teste de 1000 metros. Os testes foram comparados e analisados estatisticamente. Resultados: Após oito semanas de treinamento, o grupo experimental foi superior ao grupo de controle em todas as medidas, exceto a flexão sentado. Os resultados apresentaram alterações significativas (P<0,01). O efeito restaurador do grupo experimental foi significativamente aprimorado. Não houve diferença significativa no peso corporal, IMC, 50 metros de corrida, salto, capacidade vital, pull-up e outros indicadores antes e depois do treinamento controle (P>0,05). Conclusão: As 8 semanas de treinamento físico funcional promoveram um efeito significativo na melhoria da saúde física dos estudantes universitários. O treinamento físico funcional deve ser adequadamente reforçado nas aulas de educação física universitária para melhorar a aptidão física dos estudantes. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

ABSTRACT To explore the functions of Txnip and its mechanism in adipocyte differentiation, the preadipocytes were isolated from subcutaneous adipose tissue of three-day-old piglets and induced into adipogenic differentiation. The expression of Txnip and ChREBP was silenced, and the Txnip overexpression was achieved in the cells with transfection of the recombinant lentivirus strategies. Txnip silencing promoted the differentiation of porcine preadipocytes and PPARγ expression, and a PPARγ inhibitor reduced this facilitation. Instead, Txnip overexpression exerted a suppressive effect on the cell differentiation and PPARγ expression, and the PPARγ agonist offset this inhibition. High glucose stimulated the preadipocyte differentiation and expressions of ChREBP and Txnip. In contrast, Txnip expression was reduced by ChREBP silencing, suggesting glucose-regulated Txnip expression through the mediation of ChREBP. Moreover, the expressions of ChREBP and Glut4 induced by high glucose and glucose uptake of the cells were reduced by Txnip-overexpression, but increased by Txnip silencing, while these changes of Txnip did not alter their expressions under low glucose. Collectively, Txnip could be an inhibitor of porcine preadipocyte differentiation, which attenuated the adipogenesis through the negative feedback regulation on PPARγ. Txnip impaired the induction of glucose on the preadipocyte differentiation through decreasing Glut4 expression and glucose uptake and subsequent decrease of the expression and transcriptional activity of ChREBP. threedayold three day old silenced strategies facilitation Instead inhibition contrast glucoseregulated regulated Moreover Glut Txnipoverexpression, Txnipoverexpression overexpression, Txnip-overexpression Collectively

Abstract The object of this study was to investigate the influence of bagging or the combination of umbrella and bags on berry composition, especially anthocyanin accumulation, in ‘Kyoho’ (V. labruscana) berry skins. Five different colors and bag materials commonly used in production were chosen for this study: white, green, yellow, nonwoven and transparent, and the umbrellas were made of plastic material. The composition and content of anthocyanins in the berry skin of ‘Kyoho’ were analyzed by HPLC-MS. The results showed that bagging or a combination of umbrella and bags on anthocyanin concentration in ‘Kyoho’ grapes showed inconsistent effects that depended on the colors and materials of the bags and the use of an umbrella or not. Compared with the control, the inhibition effects of white, green and yellow bags on the soluble solids content and the total and individual anthocyanin concentrations in ‘Kyoho’ grapes reached significant levels; effects of green and yellow bags were the most significant among them. The plastic umbrella used in this study also presented a lower relative light intensity and produced more even-colored clusters. White, green, and yellow bags and combinations of umbrella and bags significantly increased some stable anthocyanin proportions to varying degrees, such as coumaroylated and methylated anthocyanins, compared to the control.

OBJECTIVE: To investigate the feasibility of using free gracilis muscle transfer along with the brachialis muscle branch of the musculocutaneous nerve to restore finger and thumb flexion in lower trunk brachial plexus injury according to an anatomical study and a case report. METHODS: Thirty formalin-fixed upper extremities from 15 adult cadavers were used in this study. The distance from the point at which the brachialis muscle branch of the musculocutaneous nerve originates to the midpoint of the humeral condylar was measured, as well as the length, diameter, course and branch type of the brachialis muscle branch of the musculocutaneous nerve. An 18-year-old male who sustained an injury to the left brachial plexus underwent free gracilis transfer using the brachialis muscle branch of the musculocutaneous nerve as the donor nerve to restore finger and thumb flexion. Elbow flexion power and hand grip strength were recorded according to British Medical Research Council standards. Postoperative measures of the total active motion of the fingers were obtained monthly. RESULTS: The mean length and diameter of the brachialis muscle branch of the musculocutaneous nerve were 52.66±6.45 and 1.39±0.09 mm, respectively, and three branching types were observed. For the patient, the first gracilis contraction occurred during the 4th month. A noticeable improvement was observed in digit flexion one year later; the muscle power was M4, and the total active motion of the fingers was 209°. CONCLUSIONS: Repairing injury to the lower trunk of the brachial plexus by transferring the brachialis muscle branch of the musculocutaneous nerve to the anterior branch of the obturator nerve using a tension-free direct suture is technically feasible, and the clinical outcome was satisfactory in a single surgical patient.

OBJECTIVE: In gracilis functioning free muscle transplantation, the limited caliber of the dominant vascular pedicle increases the complexity of the anastomosis and the risk of vascular compromise. The purpose of this study was to characterize the results of using a T-shaped vascular pedicle for flow-through anastomosis in gracilis functioning free muscle transplantation for brachial plexus injury. METHODS: The outcomes of patients with brachial plexus injury who received gracilis functioning free muscle transplantation with either conventional end-to-end anastomosis or flow-through anastomosis from 2005 to 2013 were retrospectively compared. In the flow-through group, the pedicle comprised a segment of the profunda femoris and the nutrient artery of the gracilis. The recipient artery was interposed by the T-shaped pedicle. RESULTS: A total of 46 patients received flow-through anastomosis, and 25 patients received conventional end-to-end anastomosis. The surgical time was similar between the groups. The diameter of the arterial anastomosis in the flow-through group was significantly larger than that in the end-to-end group (3.87 mm vs. 2.06 mm, respectively, p<0.001), and there were significantly fewer cases of vascular compromise in the flow-through group (2 [4.35%] vs. 6 [24%], respectively, p=0.019). All flaps in the flow-through group survived, whereas 2 in the end-to-end group failed. Minimal donor-site morbidity was noted in both groups. CONCLUSIONS: Flow-through anastomosis in gracilis functioning free muscle transplantation for brachial plexus injury can decrease the complexity of anastomosis, reduce the risk of flap loss, and allow for more variation in muscle placement.

In this paper, the notions of SPN-compactness, countable SPNcompactness and the SPN-Lindelöf property are introduced in L-topological spaces by means of strongly preclosed L-sets. In an L-space, an Lset having the SPN-Lindelöf property is SPN-compact if and only if it is countably SPN-compact. (Countable) SPN-compactness implies (countable) N-compactness, the SPN-Lindelöf property implies the N-Lindelöf property, but each inverse is not true. Every L-set with finite support is SPN-compact. The intersection of an (a countable) SPN-compact L-set and a strongly preclosed L-set is (countably) SPNcompact. The strong preirresolute image of an (a countable) SPNcompact L-set is (countably) SPN-compact. Moreover SPN-compactness can be characterized by nets