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ABSTRACT Objective: To describe the epidemiological profile of the list of patients with pediatric scoliosis (0 to 18 years old) treated at a tertiary public hospital in the Midwest Region. Methods: A cross-sectional analytical study of patients with scoliosis from the orthopedic service of a reference center was carried out, and data collected on age, sex, date of menarche, weight, height, etiology, curve classification, form of referral, treatment performed before and after care and waiting time for surgery after indication. The sample consisted of 60 patients randomly selected among those treated. Results: 60 patients were evaluated, 44 (73.3%) were female, and 16 (26.7%) were male, with a mean age of 13.4 years. Until the moment of the study, six patients underwent surgery after being treated at the reference center. Idiopathic scoliosis was the most frequent in 38 (63%) patients. No conduct had been performed in 47 (78.3%) patients before referral to the reference center. Aftercare at the reference center, surgical treatment was indicated in 44 (73.3%) patients. The mean waiting time for surgery after the indication was 22 months, ranging from 6 to 40 months. Conclusions: The list of patients with pediatric scoliosis treated at the reference center in the Midwest region is composed of young girls with idiopathic scoliosis who, for the most part, did not have the indication of using a brace before being referred to the reference center, and most of them had an indication for surgery for adequate treatment. Level of Evidence IV; Descriptive Study.

RESUMEN Objetivo: Describir el perfil epidemiológico de pacientes con escoliosis pediátrica (0 a 18 años) atendidos en un hospital público terciario de la Región Centro Oeste. Métodos: Se realizó un estudio analítico transversal de pacientes con escoliosis del servicio de ortopedia del centro de referencia y se recogieron datos sobre edad, sexo, fecha de la menarquia, peso, talla, etiología, clasificación de la curva, forma de derivación, tratamiento realizado antes y después de la atención y el tiempo de espera para la cirugía después de la indicación. La muestra estuvo constituida por 60 pacientes seleccionados aleatoriamente. Resultados: De los 60 pacientes, 44 (73,3%) eran mujeres y 16 (26,7%) hombres, con edad media de 13,4 años. Hasta el momento del estudio, seis pacientes fueron intervenidos quirúrgicamente. La escoliosis idiopática fue la más frecuente, en 38 (63%) pacientes. En 47 (78,3%) pacientes no se había realizado ninguna conducta antes de la derivación al centro de referencia. Tras la atención en el centro de referencia, se indicó tratamiento quirúrgico en 44 (73,3%) pacientes. El tiempo medio de espera de la cirugía fue de 22 meses, con un rango de 6 a 40 meses. Conclusiones: La lista de pacientes con escoliosis pediátrica atendidos en el único centro de referencia de la región Centro Oeste está compuesta por niñas jóvenes, quienes en su mayor parte no tenían indicación de uso de ortesis, y la mayoría tenía indicación de cirugía para un adecuado tratamiento. Nivel de evidencia IV; Estudio Descriptivo.

RESUMO Objetivo: Descrever o perfil epidemiológico da lista de pacientes com escoliose pediátrica (0 a 18 anos) atendidos em hospital público terciário da Região Centro-Oeste. Métodos: Foi realizado estudo analítico transversal de pacientes com escoliose do serviço de ortopedia do centro de referência e coletados dados de idade, sexo, data da menarca, peso, altura, etiologia, classificação da curva, forma de encaminhamento, tratamento realizado antes e após o atendimento e tempo de espera para cirurgia após indicação. A amostra foi composta por 60 pacientes selecionados randomicamente. Resultados: Dos 60 pacientes avaliados, 44 (73,3%) eram do sexo feminino e 16 (26,7%) do sexo masculino, com idade média de 13,4 anos. Até o momento do estudo, seis pacientes foram submetidos a cirurgia após o atendimento no centro de referência. A escoliose idiopática foi a mais frequente em 38 (63%) pacientes. Nenhuma conduta havia sido realizada em 47 (78,3%) pacientes antes do encaminhamento ao centro de referência. Após o atendimento no centro de referência, o tratamento cirúrgico foi indicado em 44 (73,3%) pacientes. O tempo médio de espera pela cirurgia após a indicação foi de 22 meses, variando de 6 a 40 meses. Conclusões: A lista de pacientes com escoliose pediátrica atendidos no único centro de referência da região Centro-oeste é composta por meninas jovens, com escoliose idiopática, que em grande parte não tiveram a indicação do uso de colete antes do encaminhamento ao centro de referência, e tiveram em sua maioria indicação de cirurgia para tratamento adequado. Nível de Evidência IV; Estudo Descritivo.

https://doi.org/10.1590/s1808-185120222203273450

The SISBIOTA-Diptera Brazilian Network: A long-term survey of Diptera from unexplored Brazilian Western Arc of Amazon, Cerrado, and Pantanal
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Lamas, Carlos José Einicker

; Fachin, Diego Aguilar

; Falaschi, Rafaela Lopes

; Alcantara, Daniel Máximo Correa de

; Ale-Rocha, Rosaly

; Amorim, Dalton de Souza

; Araújo, Maíra Xavier

; Ascendino, Sharlene

; Baldassio, Letícia

; Bellodi, Carolina Ferraz

; Bravo, Freddy

; Calhau, Julia

; Capellari, Renato Soares

; Carmo-Neto, Antonio Marcelino do

; Cegolin, Bianca Melo

; Couri, Márcia Souto

; Carvalho, Claudio José Barros de

; Dios, Rodrigo de Vilhena Perez

; Falcon, Aida Vanessa Gomez

; Fusari, Livia Maria

; Garcia, Carolina de Almeida

; Gil-Azevedo, Leonardo Henrique

; Gomes, Marina Morim

; Graciolli, Gustavo

; Gudin, Filipe Macedo

; Henriques, Augusto Loureiro

; Krolow, Tiago Kütter

; Mendes, Luanna Layla

; Limeira-de-Oliveira, Francisco

; Maia, Valéria Cid

; Marinoni, Luciane

; Mello, Ramon Luciano

; Mello-Patiu, Cátia Antunes de

; Morales, Mírian Nunes

; Oliveira, Sarah Siqueira

; Patiu, Claudemir

; Proença, Barbara

; Pujol-Luz, Cristiane Vieira de Assis

; Pujol-Luz, José Roberto

; Rafael, José Albertino

; Riccardi, Paula Raile

; Rodrigues, João Paulo Vinicios

; Roque, Fabio de Oliveira

; Sallum, Maria Anice Mureb

; Santis, Marcelo Domingos de

; Santos, Charles Morphy Dias dos

; Santos, Josenilson Rodrigues dos

; Savaris, Marcoandre

; Shimabukuro, Paloma Helena Fernandes

; Silva, Vera Cristina

; Schelesky-Prado, Daniel de Castro

; Silva-Neto, Alberto Moreira da

; Camargo, Alexssandro

; Sousa, Viviane Rodrigues de

; Urso-Guimarães, Maria Virginia

; Wiedenbrug, Sofia

; Yamaguchi, Carolina

; Nihei, Silvio Shigueo

Revista Brasileira de Entomologia

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2023, Volume 67 N. 4 elocation e20230051

Abstract: En | Text: En | PDF: En

ABSTRACT The SISBIOTA-BRASIL was a three-year multimillion-dollar research program of the Brazilian government to document plants and animals in endangered/understudied areas and biomes in Brazil. Distributional patterns and the historical events that generated them are extensively unknown regarding Brazilian fauna and flora. This deficiency hinders the development of conservation policies and the understanding of evolutionary processes. Conservation decisions depend on precise knowledge of the taxonomy and geographic distribution of species. Given such a premise, we proposed to research the diversity of Diptera of the Brazilian western arc of Amazon, Cerrado, and Pantanal in the states of Mato Grosso, Mato Grosso do Sul, and Rondônia. Three important biomes of the South American continent characterize these Brazilian states: Amazon forest, Cerrado (Brazilian Savannah), and Pantanal. Besides their ecological relevance, these biomes historically lack intensive entomological surveys. Therefore, they are much underrepresented in the Brazilian natural history collections and in the scientific literature, which is further aggravated by the fact that these areas are being exponentially and rapidly converted to commercial lands. Our project involved over 90 collaborators from 24 different Brazilian institutions and one from Colombia among researchers, postdocs, graduate and undergraduate students, and technicians. We processed and analyzed nearly 300,000 specimens from ~60 families of Diptera collected with a large variety of methods in the sampled areas. Here, we provide a detailed overview of the genera and species diversity of 41 families treated. Our results point to a total of 2,130 species and 514 genera compiled and identified for the three states altogether, with an increase of 41% and 29% in the numbers of species and genera known for the three states combined, respectively. Overall, the 10 most species-rich families were Tachinidae, Cecidomyiidae, Tabanidae, Psychodidae, Sarcophagidae, Stratiomyidae, Bombyliidae, Syrphidae, Tephritidae, and Asilidae. The 10 most diverse in the number of genera were Tachinidae, Stratiomyidae, Asilidae, Mycetophilidae, Syrphidae, Tabanidae, Muscidae, Dolichopodidae, Sarcophagidae, and Chloropidae. So far, 111 scientific papers were published regarding taxonomic, phylogenetic, and biogeographical aspects of the studied families, with the description of 101 new species and three new genera. We expect that additional publications will result from this investigation because several specimens are now curated and being researched by specialists.

https://doi.org/10.1590/1806-9665-rbent-2023-0051

Recommendations by the Scientific Department of Neuroimmunology of the Brazilian Academy of Neurology (DCNI/ABN) and the Brazilian Committee for Treatment and Research in Multiple Sclerosis and Neuroimmunological Diseases (BCTRIMS) on vaccination in general and specifically against SARS-CoV-2 for patients with demyelinating diseases of the central nervous system
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Becker, Jefferson

; Ferreira, Lis Campos

; Damasceno, Alfredo

; Bichuetti, Denis Bernardi

; Christo, Paulo Pereira

; Callegaro, Dagoberto

; Peixoto, Marco Aurélio Lana

; Sousa, Nise Alessandra De Carvalho

; Almeida, Sérgio Monteiro De

; Adoni, Tarso

; Santiago-Amaral, Juliana

; Junqueira, Thiago

; Pereira, Samira Luisa Apóstolos

; Gomes, Ana Beatriz Ayroza Galvão Ribeiro

; Pitombeira, Milena

; Paolilo, Renata Barbosa

; Grzesiuk, Anderson Kuntz

; Piccolo, Ana Claudia

; D´Almeida, José Arthur Costa

; Gomes Neto, Antonio Pereira

; Oliveira, Augusto Cesar Penalva De

; Oliveira, Bianca Santos De

; Tauil, Carlos Bernardo

; Vasconcelos, Claudia Ferreira

; Kaimen-Maciel, Damacio

; Varela, Daniel

; Diniz, Denise Sisterolli

; Oliveira, Enedina Maria Lobato De

; Malfetano, Fabiola Rachid

; Borges, Fernando Elias

; Figueira, Fernando Faria Andrade

; Gondim, Francisco De Assis Aquino

; Passos, Giordani Rodrigues Dos

; Silva, Guilherme Diogo

; Olival, Guilherme Sciascia Do

; Santos, Gutemberg Augusto Cruz Dos

; Ruocco, Heloisa Helena

; Sato, Henry Koiti

; Soares Neto, Herval Ribeiro

; Cortoni Calia, Leandro

; Gonçalves, Marcus Vinícius Magno

; Vecino, Maria Cecilia Aragón De

; Pimentel, Maria Lucia Vellutini

; Ribeiro, Marlise De Castro

; Boaventura, Mateus

; Parolin, Mônica Koncke Fiuza

; Melo, Renata Brant De Souza

; Lázaro, Robson

; Thomaz, Rodrigo Barbosa

; Kleinpaul, Rodrigo

; Dias, Ronaldo Maciel

; Gomes, Sidney

; Lucatto, Simone Abrante

; Alves-Leon, Soniza Vieira

; Fukuda, Thiago

; Ribeiro, Taysa Alexandrino Gonsalves Jubé

; Winckler, Thereza Cristina D’ávila

; Fragoso, Yara Dadalti

; Nascimento, Osvaldo José Moreira Do

; Ferreira, Maria Lucia Brito

; Mendes, Maria Fernanda

; Brum, Doralina Guimarães

; Glehn, Felipe Von

Arquivos de Neuro-Psiquiatria

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nov 2021, Volume 79 N. 11 Pages 1049 - 1061

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O DC de Neuroimunologia da ABN e o BCTRIMS trazem, nesse documento, as recomendações sobre vacinação da população com doenças desmielinizantes do sistema nervoso central (SNC) contra infecções em geral e contra o coronavírus da síndrome respiratória aguda grave 2 (SARS-CoV-2), causador da COVID-19. Destaca-se a gravidade do atual momento frente ao avanço da COVID-19 em nosso País, o que torna mais evidente e importante a criação de guia de referência para orientação aos médicos, pacientes e autoridades de saúde pública quanto à vacinação, meio efetivo e seguro no controle de determinadas doenças infecciosa. O DCNI/ABN e o BCTRIMS recomendam que os pacientes com doenças desmielinizantes do SNC (ex., EM e NMOSD) sejam constantemente monitorados, quanto a atualização do seu calendário vacinal, especialmente, no início ou antes da mudança do tratamento com uma droga modificadora de doença (DMD). É importante também salientar que as vacinas são seguras e os médicos devem estimular o seu uso em todos os pacientes. Evidentemente, deve ser dada especial atenção às vacinas com vírus vivos atenuados. Por fim, é importante que os médicos verifiquem qual DMD o paciente está em uso e quando foi feita a sua última dose, pois cada fármaco pode interagir de forma diferente com a indução da resposta imune.

ABSTRACT The Scientific Department of Neuroimmunology of the Brazilian Academy of Neurology (DCNI/ABN) and Brazilian Committee for Treatment and Research in Multiple Sclerosis and Neuroimmunological Diseases (BCTRIMS) provide recommendations in this document for vaccination of the population with demyelinating diseases of the central nervous system (CNS) against infections in general and against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes COVID-19. We emphasize the seriousness of the current situation in view of the spread of COVID-19 in our country. Therefore, reference guides on vaccination for clinicians, patients, and public health authorities are particularly important to prevent some infectious diseases. The DCNI/ABN and BCTRIMS recommend that patients with CNS demyelinating diseases (e.g., MS and NMOSD) be continually monitored for updates to their vaccination schedule, especially at the beginning or before a change in treatment with a disease modifying drug (DMD). It is also important to note that vaccines are safe, and physicians should encourage their use in all patients. Clearly, special care should be taken when live attenuated viruses are involved. Finally, it is important for physicians to verify which DMD the patient is receiving and when the last dose was taken, as each drug may affect the induction of immune response differently.

https://doi.org/10.1590/0004-282x-anp-2021-0162 1 downloads

MicroRNA-27b Expression in Rats Submitted to Physical Exercise and Experimental Cerebral Ischemia
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De-Russi, Brenno Marco

; Porsani, Lucas Barbosa

; Cirino, Mucio Luiz de Assis

; Silva, Jairo Pinheiro da

; Turra, Letícia Passi

; Novais, Paulo Cezar

; Lizarte-Neto, Fermino Sanches

; Fazan, Valéria de Paula Sassoli

; Thomazini, José Antônio

; Colli, Benedicto Oscar

; Tirapelli, Daniela Pretti da Cunha

; Tirapelli, Luis Fernando

; Carvalho, Camila Albuquerque Melo de

International Journal of Morphology

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jun 2021, Volume 39 N. 3 Pages 754 - 758

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

SUMMARY: Cerebral ischemia has not only a high mortality rate, which is the second leading cause of death worldwide, but is also responsible for severe disabilities in working age individuals, generating enormous public expending for treatment and rehabilitation of the affected individuals. The role of microRNAs in the pathophysiology of cerebral ischemia has been highlighted in current investigations. In addition, recent studies have also highlighted physical exercise as a possible protective factor both in the prevention and in the effects of cerebral ischemia, placing it as an important study resource. Thus, we investigated the role of physical exercise in experimental cerebral ischemia associated with the expression of microRNA-27b. 16 animals were used, divided into four experimental groups: Control, Physical Exercise, Cerebral Ischemia and Cerebral Ischemia associated with Physical Exercise. The real-time PCR methodology was used to analyze the expression of microRNA-27b. Although there were no statistically significant differences in the expression of microRNA-27b between the groups studied, the increased expression of microRNA-27b in the Physical Exercise group indicates its neuroprotective role in the pathophysiology of cerebral ischemia.

RESUMEN: La isquemia cerebral no solo tiene una alta tasa de mortalidad y es la segunda causa principal de muerte en todo el mundo, sino también es la causa de enfermedades invalidantes en personas en edad laboral, lo que genera un gasto público enorme para el tratamiento y la rehabilitación de las personas afectadas. El papel de los microARN en la fisiopatología de la isquemia cerebral se ha destacado en las investigaciones actuales. Además, estudios recientes también han destacado el ejercicio físico como un posible factor protector tanto en la prevención como en los efectos de la isquemia cerebral, situándolo como un importante recurso de estudio. Por lo tanto, investigamos el papel del ejercicio físico en la isquemia cerebral experimental asociada con la expresión del microARN-27b. Se utilizaron 16 animales, divididos en cuatro grupos experimentales: Control, Ejercicio Físico, Isquemia Cerebral e Isquemia Cerebral asociada al Ejercicio Físico. Se utili- zó la metodología de PCR en tiempo real para analizar la expresión de microARN-27b. Aunque no se observaron diferencias estadísticamente significativas en la expresión de microARN-27b entre los grupos estudiados, la mayor expresión de microARN-27b en el grupo de Ejercicio Físico indica su papel neuroprotector en la fisiopatología de la isquemia cerebral.

75 downloads

Rendimento gravimétrico da carbonização e caracterização qualitativa do carvão vegetal em clones de híbridos de Corymbia spp para uso industrial
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Loureiro, Breno Assis

; Assis, Maíra Reis de

; Melo, Isabel Cristina Nogueira Alves de

; Oliveira, Ana Flávia Fernandes de

; Trugilho, Paulo Fernando

Ciência Florestal

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mar 2021, Volume 31 N. 1 Pages 214 - 232

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Na presente pesquisa, objetivou-se avaliar os rendimentos gravimétricos da carbonização e as características qualitativas do carvão vegetal de clones de híbridos obtidos dos cruzamentos de espécies de Corymbia, e identificar os mais adequados para uso siderúrgico. Seis clones, aos 6 anos e 5 meses de idade, foram utilizados na pesquisa e amostradas cinco árvores de cada material genético. As carbonizações laboratoriais foram realizadas à temperatura final de 450°C, taxa de aquecimento de 100°C.h-1 e tempo de residência de 30 minutos. Os rendimentos gravimétricos da carbonização foram determinados em relação à madeira seca. O carvão produzido foi qualificado, considerando-se a análise química imediata, poder calorífico superior, densidade energética, consumo específico de madeira e a eficiência energética. Na avaliação do experimento, foi utilizado o delineamento inteiramente casualizado com cinco repetições (árvores). A análise multivariada de componentes principais foi empregada para obter a variância dos vetores normalizados, considerando as características avaliadas nos carvões. Os resultados obtidos indicaram o elevado potencial para a utilização dos materiais genéticos de Corymbia, para fins energéticos, sobretudo para a produção de carvão vegetal siderúrgico. O clone 1 foi o destaque para a produção de carvão vegetal e lenha, pois apresenta maiores densidades aparentes e energéticas, maior teor e rendimento em carbono fixo, além de possuir menor consumo específico de madeira por tonelada de carvão vegetal produzido. Os clones 2 e 4 foram os que mais se distinguiram dos demais, respectivamente, em decorrência de seu maior teor de cinzas e por apresentar maior consumo específico de madeira na produção de carvão. O uso de clones híbridos de Corymbia, para a produção de carvão vegetal, pode ser uma boa alternativa, em razão de seu rápido crescimento e qualidades semelhantes de madeira e carvão vegetal a outras espécies que já são usadas para a finalidade energética.

ABSTRACT This research aimed to evaluate the carbonization gravimetric yields and qualitative characteristics of the charcoal of hybrid clones obtained from the crossings of Corymbia species and to identify the most suitable for the steel industry. We used six clones, at six years and five months of age, sampling five trees of each genetic material. The laboratory carbonizations consisted of a final temperature of 450°C, a heating rate of 100°C.h-1, and a residence time of 30 minutes. We determined the carbonization gravimetric yields when compared to dry wood. The charcoal produced was qualified considering the proximate chemical analysis, superior calorific value, energy density, specific wood consumption, and energy efficiency. We organized the experiment in a completely randomized design with five replicates (trees). The multivariate principal component analysis determined the variance of the normalized vectors, considering the charcoal characteristics evaluated. The results indicated a high potential for the use of Corymbia genetic materials for energy purposes, especially for producing charcoal for the steel industry. Clone 1 was prominent for producing charcoal and firewood, given its higher apparent and energetic densities, fixed carbon content and yield, and lower specific wood consumption per ton of charcoal produced. Clones 2 and 4 most distinguished themselves from the others due to higher ash content and higher specific wood consumption, respectively. The use of Corymbia hybrid clones for producing charcoal may be a good alternative due to its rapid growth and wood and charcoal characteristics similar to those of other species that are already used for energy purposes.

https://doi.org/10.5902/1980509836120 163 downloads

Composição físico-química e compostos bioativos do extrato aquoso de amendoim sem pele e enriquecido com pele
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Oliveira, Thárcia Kiara Beserra de

; Almeida, Francisco de Assis Cardoso

; Gomes, Josivanda Palmeira

; Lima, Amélia Ruth Nascimento

; Melo Neto, Inácio de Barros

; Silva Júnior, Paulo Roberto da

; Ramos, Katharina Rodrigues de Lima Porto

Brazilian Journal of Food Technology

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2021, Volume 24 elocation e2020166

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Objetivou-se realizar a caracterização físico-química e dos compostos bioativos do extrato aquoso de amendoim sem pele e acrescido de 1% de pele. Os extratos aquosos de amendoim sem e com pele foram caracterizados quanto aos parâmetros: umidade, extrato seco total, cinzas, lipídios, proteínas, teor de sólidos solúveis, acidez titulável, pH, carboidrato e fibras totais, além de compostos fenólicos e flavonoides. Os percentuais de umidade encontrados foram elevados em todas as amostras, resultante de sua base de extrato aquoso produzido na proporção 1:8 (amendoim:água). Observou-se que os extratos aquosos de amendoim sem pele e acrescido de 1% de pele não apresentaram diferença significativa entre si para os parâmetros de pH, como também para sólidos solúveis totais, lipídios e carboidratos. No entanto, a acidez, proteínas, fibras e cinzas apresentaram diferença estatística. Na determinação de compostos fenólicos, notou-se que o extrato aquoso de amendoim com pele apresentou uma quantidade maior quando comparado ao sem pele. Quanto aos flavonoides, a maior quantidade também foi encontrada na amostra com pele, apresentando uma diferença média de 2,52 µg/100 g base úmida. Os extratos aquosos de amendoim com e sem pele mostraram ser uma valiosa alternativa para um melhor aproveitamento do amendoim, proporcionando à população um produto nutricional. A bebida acrescida de 1% de pele enriqueceu o produto quanto ao potencial antioxidante devido às suas maiores concentrações de compostos fenólicos totais e flavonoides.

Abstract The objective of this work was to carry out the physical-chemical characterization and bioactive compounds of aqueous extract of peanuts without skin and with addition of 1% of skin. The aqueous extracts of peanuts without and with skin were characterized according to the parameters: moisture, total dry extract, ash, lipids, proteins, soluble solids content, titratable acidity, pH, carbohydrates and total fibers, in addition to phenolic and flavonoid compounds. The percentages of moisture found were high in all samples, resulting from its base of aqueous extract produced in the proportion 1:8 (peanut:water). It was observed that the aqueous extracts of peanuts without skin and the addition of 1% of skin did not present significant difference between them for the pH parameters, as well as for total soluble solids, lipids and carbohydrates. However, acidity, proteins, fibers and ash presented a statistical difference. In the determination of phenolic compounds, it was noted that the aqueous extract of peanuts with skin presented a larger amount when compared to those without skin. As for flavonoids, the largest amount was also found in the skin sample, obtaining an average difference of 2.52 µg/100 g wet basis. The aqueous extract of peanuts with and without skin proved to be a valuable alternative for a better use of peanuts, providing the population with a nutritional product. The beverage added with 1% of skin enriches the product in terms of its antioxidant potential, due to its greater concentrations of phenolic compounds and flavonoids.

https://doi.org/10.1590/1981-6723.16620 284 downloads

Morphometric Analysis and Pattern of Protein and Gene Expression of Apoptosis in Focal Cerebral Ischemia in Rats and the Neuroprotetive Action of Hypothermia and Ketoprofen
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Tirapelli, Daniela P. C

; Cirino, Mucio Luiz de Assis

; Carvalho, Camila A. Melo de

; Novais, Paulo Cezar

; Figueredo, Rafael Zimak

; Porsani, Lucas Barbosa

; Gula, Isabella Stracieri

; Rodrigues, Andressa Romualdo

; Silva, Jairo Pinheiro da

; Pereira, Adriana Lis

; Lizarte Neto, Fermino Sanches

; Schimming, Bruno Cesar

; Tazima, Maria de Fátima Galli Sorita

; Fazan, Valéria de Paula Sassoli

; Carlotti-Jr, Carlos Gilberto

; Tirapelli, Luis Fernando

; Colli, Benedicto Oscar

International Journal of Morphology

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jun 2020, Volume 38 N. 3 Pages 523 - 529

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

SUMMARY: This study aimed to investigate the morphometric and the pattern of protein and gene expression related to the extrinsic apoptotic pathway in experimental focal cerebral ischemia and the hole of neuroprotection with hypothermia and ketoprofen. For this analysis, 120 rats were randomly divided into 3 groups (20 animals each): control - no surgery (20 animals); sham - simulation of surgery (20 animals); ischemic - focal ischemia for 1 hour, without reperfusion (80 animals) and divided into four subgroups with 20 animals each: ischemic + intraischemic hypothermia; ischemic + previous intravenous ketoprofen, and ischemic + hypothermia and ketoprofen. The infarct volume was measured using morphometric analysis of infarct areas defined by triphenyl tetrazolium chloride and the patterns of expression of the apoptosis genes (Fas, c-Flip, caspase-8 and caspase-3) and the apoptosis protein caspase-3 were evaluated by quantitative real-time PCR and immunohistochemistry, respectively. Hypo expression of genes of extrinsic pathway of apoptosis was observed: Fas receptor, c-Flip and caspase-8 in the ischemics areas. Increases in the gene and protein caspase-3 in the ischemic areas were also observed, and these increases were reduced by hypothermia and ketoprofen, also noted in the morphometric study. The caspases-3 increase suggests that this gene plays an important role in apoptosis, probably culminating in cell death and that the neuroprotective effect of hypothermia and ketoprofen is involved.

RESUMEN: Este estudio tuvo como objetivo investigar la morfometría y el patrón de expresión de proteínas y genes relacionados con la vía apoptótica extrínseca en la isquemia cerebral focal experimental y el agujero de neuroprotección con hipotermia y ketoprofeno. Se dividieron aleatoriamente 120 ratas en 3 grupos (20 animales cada uno): control - sin cirugía (20 animales); simulación - simulación de cirugía (20 animales); isquemia isquemia focal durante 1 hora, sin reperfusión (80 animales) y dividida en cuatro subgrupos con 20 animales cada uno: isquemia + hipotermia intraisquémica; isquemia + ketoprofeno intravenoso previo, e isquemia + hipotermia y ketoprofeno. El volumen del infarto se midió utilizando un análisis morfométrico de áreas de infarto definidas por cloruro de trifenil tetrazolio y los patrones de expresión de los genes de apoptosis (Fas, c-Flip, caspase-8 y caspase-3) y la proteína de apoptosis caspase-3 fueron evaluados por PCR cuantitativa en tiempo real e inmunohistoquímica, respectivamente. Se observó hipoexpresión de genes de la vía extrínseca de la apoptosis: receptor Fas, c-Flip y caspasa-8 en las áreas isquémicas. También se observaron aumentos en el gen y la proteína caspasa-3 en las áreas isquémicas y estos aumentos se redujeron por hipotermia y ketoprofeno, también observado por estudio morfométrico. El aumento de caspasas-3 sugiere que este gen tiene un papel importante en la apoptosis, y probable causa de muerte celular, involucrando el efecto neuroprotector de la hipotermia y el ketoprofeno.

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Salt stress alleviation by seed priming with silicon in lettuce seedlings: an approach based on enhancing antioxidant responses
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Alves, Rita de Cássia

; Nicolau, Mayara Cristina Malvas

; Checchio, Mirela Vantini

; Sousa Junior, Gilmar da Silveira

; Oliveira, Francisco de Assis de

; Prado, Renato Melo

; Gratão, Priscila Lupino

Bragantia

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mar 2020, Volume 79 N. 1 Pages 19 - 29

Abstract: En | Text: En | PDF: En

ABSTRACT Seed germination and seedling growth are the stages most sensitive to salt stress, which can induce ion-specific effects and oxidative stress. Although silicon (Si) has been known to improve plant defense systems against stressful-conditions, little is known about seed priming with Si and mechanisms of Si-mediated alleviation of salt stress in seedlings. To further the modulation of salt-stress responses and their relation with Si, seed priming with calcium silicate (0, 0.05 and 0.1 mM Ca2SiO4) was used in lettuce seeds to improve seedgermination and tolerance on subsequent salt-stress exposure (0 and 50 mM NaCl). The effect of Si-priming was assessed on germination, germination rate index and mean germination time and survival of seedlings in salt-stressful conditions supported by biochemical approach involving antioxidant responses. Overall results indicated that Si plays a role in alleviating the negative effects of salt stress by improving germination performance and displayed increased SOD, CAT and GR activities and reduced malondialdehyde and H2O2 contents. Thus, our findings demonstrate that seed-priming with Si is an efficient management technique thatcan be used to alleviate deleterious effects of salt-stressful on germination of lettuce seeds and enhance salt tolerance of seedlings due the increased activity of ROS- scavenging enzymes.

https://doi.org/10.1590/1678-4499.20190360 1094 downloads

Chronic alcoholism associated with diabetes induced apoptosis in the corpus cavernosum of rats
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Schimming, Bruno Cesar

; Cirino, Mucio Luiz de Assis

; Lizarte Neto, Fermino Sanches

; Novais, Paulo Cezar

; Carvalho, Camila Albuquerque Melo de

; Tirapelli, Daniela Pretti da Cunha

; Molina, Carlos Augusto Fernandes

; Tirapelli, Luis Fernando

; Tucci Junior, Silvio

Acta Cirúrgica Brasileira

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2020, Volume 35 N. 8 elocation e202000805

Abstract: En | Text: En | PDF: En

Abstract Purpose To evaluate the effects of alcohol exposure and diabetes on apoptotic process in the corpus cavernosum. Methods Forty eight male Wistar rats were divided into four groups: control, diabetic, alcoholic and diabetic-alcoholic. Samples of the corpus cavernosum were prepared to study protein expression of apoptotic genes (Caspases-3 and 9) by immunohistochemistry and Real-Time PCR. Results The immunoreactivity of Caspases-3 and -9 was diffuse and higher in the treated groups though there was no significant difference between the experimental groups, only when compared with the control group. An increase was observed in the gene expression of Caspases-9 in the diabetic and ethanol-diabetic groups when compared with control and ethanol groups. Conclusions The association of these factors (ethanol and diabetes) probably can affect the apoptosis mechanism in lesions of the cavernous tissue in the rat penis. Both gene and protein expression of Caspase-9 in diabetic and ethanol-diabetic groups suggest the involvement of the apoptosis cascade from this study model.

https://doi.org/10.1590/s0102-865020200080000005 723 downloads

10.

Detection of Drug Resistant Mycobacterium Tuberculosis Strains Using Kit SIRE Nitratase®: a Multicenter Study
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Miranda, Silvana Spindola de

; Almeida, Isabela Neves de

; Mansur, Maria de Fátima Filardi Oliveira

; Figueredo, Lida Jouca de Assis

; Carvalho, Wânia da Silva

; Hadaad, João Paulo Amaral

; Diniz, Jaciara de Lourdes do Carmo Guimarães

; Groll, Andrea von

; Silva, Pedro Almeida da

; Lopes, Maria Luiza

; Santos, Marcelo Cordeiro dos

; Brito, Alexandra

; Mello, Fernanda Carvalho de Queiroz

; Malaquias, Thiago da Silva Santos

; Croda, Julio

; Pinhata, Juliana Maira Watanabe

; Oliveira, Rosângela Siqueira de

; Chimara, Erica

; Rossetti, Maria Lúcia

; Halon, Maria Laura

; Lourenço, Maria Cristina

; Medeiros, Reginalda Ferreira de Melo

; Montes, Fátima Cristina Onofre Fandinho

; Machado, Diana

; Viveiros, Miguel

; Kritski, Afrânio Lineu

Brazilian Archives of Biology and Technology

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2020, Volume 63 elocation e20190179

Abstract: En | Text: En | PDF: En

Abstract (1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.

https://doi.org/10.1590/1678-4324-2020190179 905 downloads

11.

Morphological and molecular analysis of apoptosis in the corpus cavernosum of rats submitted to a chronic alcoholism model
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Meirelles, Rogério José de Azevedo

; Lizarte Neto, Fermino Sanches

; Cirino, Mucio Luiz de Assis

; Novais, Paulo Cezar

; Gula, Isabella Stracieri

; Silva, Jairo Pinheiro da

; Tazzima, Maria de Fátima Galli Sorita

; Fazan, Valéria Paula Sassoli

; Durand, Marina Toledo

; Tirapelli, Daniela Pretti da Cunha

; Carvalho, Camila Albuquerque Melo de

; Schimming, Bruno César

; Molina, Carlos Augusto Fernandes

; Tucci Junior, Silvio

; Tirapelli, Luis Fernando

Acta Cirúrgica Brasileira

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2020, Volume 35 N. 3 elocation e202000305

Abstract: En | Text: En | PDF: En

Abstract Purpose To evaluate the effect of chronic alcoholism on morphometry and apoptosis mechanism and correlate with miRNA-21 expression in the corpus cavernosum of rats. Methods Twenty-four rats were divided into two experimental groups: Control (C) and Alcoholic group (A). After two weeks of an adaptive phase, rats from group A received only ethanol solution (20%) during 7 weeks. The morphometric and caspase-3 immunohistochemistry analysis were performed in the corpus cavernosum. The miRNA-21 expression was analyzed in blood and cavernous tissue. Results Chronic ethanol consumption decreased cavernosal smooth muscle area of alcoholic rats. The protein expression of caspase 3 in the corpus cavernosum was higher in A compared to the C group. There was no difference in the expression of miRNA-21 in serum and cavernous tissue between the groups. Conclusion Chronic ethanol consumption reduced smooth muscle area and increased caspase 3 in the corpus cavernosum of rats, without altered serum and cavernosal miR-21 gene expression.

https://doi.org/10.1590/s0102-865020200030000005 715 downloads

12.

Atualização das Diretrizes em Cardiogeriatria da Sociedade Brasileira de Cardiologia – 2019
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Feitosa Filho, Gilson Soares

; Peixoto, José Maria

; Pinheiro, José Elias Soares

; Afiune Neto, Abrahão

; Albuquerque, Afonso Luiz Tavares de

; Cattani, Álvaro César

; Nussbacher, Amit

; Camarano, Ana Amelia

; Sichinels, Angela Hermínia

; Sousa, Antonio Carlos Sobral

; Alencar Filho, Aristóteles Comte de

; Gravina, Claudia F.

; Sobral Filho, Dario Celestino

; Pitthan, Eduardo

; Costa, Elisa Franco de Assis

; Duarte, Elizabeth da Rosa

; Freitas, Elizabete Viana de

; Moriguchi, Emilio Hideyuki

; Mesquita, Evandro Tinoco

; Fernandes, Fábio

; Fuchs, Felipe Costa

; Feitosa, Gilson Soares

; Pierre, Humberto

; Pereira Filho, Ilnei

; Helber, Izo

; Borges, Jairo Lins

; Garcia, Jéssica Myrian de Amorim

; Souza, José Antonio Gordillo de

; Zanon, José Carlos da Costa

; Alves, Josmar de Castro

; Mohallem, Kalil Lays

; Chaves, Laura Mariana de Siqueira Mendonça

; Moura, Lídia Ana Zytynski

; Silva, Márcia Cristina Amélia da

; Toledo, Maria Alice de Vilhena

; Assunção, Maria Elisa Lucena Sales de Melo

; Wajngarten, Mauricio

; Gonçalves, Mauro José Oliveira

; Lopes, Neuza Helena Moreira

; Rodrigues, Nezilour Lobato

; Toscano, Paulo Roberto Pereira

; Rousseff, Pedro

; Maia, Ricardo Antonio Rosado

; Franken, Roberto Alexandre

; Miranda, Roberto Dischinger

; Gamarski, Roberto

; Rosa, Ronaldo Fernandes

; Santos, Silvio Carlos de Moraes

; Galera, Siulmara Cristina

; Grespan, Stela Maris da Silva

; Silva, Teresa Cristina Rogerio da

; Esteves, William Antonio de Magalhães

Arquivos Brasileiros de Cardiologia

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may 2019, Volume 112 N. 5 Pages 649 - 705

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.5935/abc.20190086 15622 downloads

13.

Mangrove endophyte promotes reforestation tree (Acacia polyphylla) growth
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Castro, Renata Assis

; Dourado, Manuella Nóbrega

; Almeida, Jaqueline Raquel de

; Lacava, Paulo Teixeira

; Nave, André

; Melo, Itamar Soares de

; Azevedo, João Lucio de

; Quecine, Maria Carolina

Brazilian Journal of Microbiology

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mar 2018, Volume 49 N. 1 Pages 59 - 66

Abstract: En | Text: En | PDF: En

ABSTRACT Mangroves are ecosystems located in the transition zone between land and sea that serve as a potential source of biotechnological resources. Brazil's extensive coast contains one of the largest mangrove forests in the world (encompassing an area of 25,000 km2 along all the coast). Endophytic bacteria were isolated from the following three plant species: Rhizophora mangle, Laguncularia racemosa and Avicennia nitida. A large number of these isolates, 115 in total, were evaluated for their ability to fix nitrogen and solubilize phosphorous. Bacteria that tested positive for both of these tests were examined further to determine their level of indole acetic acid production. Two strains with high indole acetic acid production were selected for use as inoculants for reforestation trees, and then the growth of the plants was evaluated under field conditions. The bacterium Pseudomonas fluorescens (strain MCR1.10) had a low phosphorus solubilization index, while this index was higher in the other strain used, Enterobacter sp. (strain MCR1.48). We used the reforestation tree Acacia polyphylla. The results indicate that inoculation with the MCR1.48 endophyte increases Acacia polyphylla shoot dry mass, demonstrating that this strain effectively promotes the plant's growth and fitness, which can be used in the seedling production of this tree. Therefore, we successfully screened the biotechnological potential of endophyte isolates from mangrove, with a focus on plant growth promotion, and selected a strain able to provide limited nutrients and hormones for in plant growth.

https://doi.org/10.1016/j.bjm.2017.04.002 1427 downloads

14.

Cost-related underuse of medications in older adults: ELSI-Brazil
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Loyola Filho, Antônio Ignácio de

; Firmo, Josélia Oliveira Araújo

; Mambrini, Juliana Vaz de Melo

; Peixoto, Sérgio Viana

; Souza Junior, Paulo Roberto Borges de

; Andrade, Fabíola Bof de

; Lima-Costa, Maria Fernanda

; Acúrcio, Francisco de Assis

Revista de Saúde Pública

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2018, Volume 52 Suppl. 2 elocation 8s

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO OBJETIVO Determinar a prevalência e os fatores associados à subutilização de medicamentos por motivos financeiros em amostra nacional representativa da população brasileira com 50 anos ou mais. MÉTODOS Entre 9.412 participantes do Estudo Longitudinal sobre a Saúde dos Idosos Brasileiros (ELSI-Brasil), 6.014 informaram usar medicamento(s) de uso contínuo e foram incluídos na análise. A subutilização de medicamentos foi definida como ter, por motivos financeiros, deixado de tomar ou ter diminuído o número de comprimidos ou a dose de algum medicamento receitado pelo médico. O marco teórico empregado para a seleção das variáveis exploratórias incluiu fatores predisponentes, capacitantes e de necessidade. As associações foram testadas por meio de regressão de Poisson. RESULTADOS A prevalência de subutilização de medicamentos foi de 10,6%. Após ajustes pertinentes, associações positivas e estatisticamente significantes (p < 0,05) com o desfecho foram observadas para o sexo feminino [razão de prevalência (RP) = 1,39]; renda familiar às vezes (RP = 1,74) e nunca (RP = 2,94) suficiente para as despesas; frequência com que o médico explica sobre a doença e tratamento (RP = 1,31 para raramente ou nunca); número de medicamentos utilizados (RP = 1,39 para 2–4 e 1,53 para 5 ou mais); autoavaliação da saúde razoável (RP = 2,02) e ruim ou muito ruim (RP = 2,92); e diagnóstico médico de depressão (RP = 1,69). Associações negativas foram observadas para idade igual a 60–79 (RP = 0,75) e 80 anos ou mais (RP = 0,43), posição socioeconômica do domicílio (RP = 0,70; 0,79 e 0,60 para o segundo, terceiro e quartil superior) e cobertura por plano privado de saúde (RP = 0,79). Não foram observadas associações entre hipertensão e diabetes autorreferidos e subutilização de medicamentos. CONCLUSÕES A subutilização de medicamentos por motivos financeiros tem caráter multidimensional e complexo, abrangendo características sociodemográficas, de condições de saúde e de utilização de serviços de saúde. Esclarecer ao paciente sobre a doença e o seu tratamento, e ampliar o acesso universal à assistência farmacêutica, podem minimizar os riscos da subutilização.

ABSTRACT OBJECTIVE To assess the prevalence and factors associated with cost-related underuse of medications in a nationally representative sample of Brazilians aged 50 years and over. METHODS Among the 9,412 participants of the Brazilian Longitudinal Study of Aging (ELSI-Brazil), 6,014 reported using at least one medication on regular basis and were included in the analysis. Underuse of medications was by stopping taking or reducing the number of tablets or the dose of any prescribed medication for financial reasons. The theoretical framework used for the selection of the exploratory variables included predisposing factors, enabling factors, and factors of need. Associations were tested by Poisson regression. RESULTS The prevalence of underuse of medications was 10.6%. After adjustments for relevant covariables, positive and statistically significant associations (p < 0.05) with the outcome were found for females [prevalence ratio (PR) = 1.39], sufficiency of the family income for expenses (PR = 1.74 for sometimes and PR 2.42 for never), frequency with which the physician explains about the disease and treatment (PR = 1.31 for rarely or never), number of medications used (PR = 1.39 for 2–4 and 1.53 for 5 or more), fair (PR = 2.02) and poor or very poor self-rated health (PR = 2.92), and a previous medical diagnosis of depression (PR = 1.69). Negative associations were observed for the age groups of 60–79 years (PR = 0.75) and 80 years and over (PR = 0.43), socioeconomic status of the household (PR = 0.70, 0.79, and 0.60 for the second, third, and fourth quartile, respectively), and private health plan coverage (PR = 0.79). There were no associations between hypertension and self-reported diabetes and underuse of medications. CONCLUSIONS Cost-related underuse of medications is multidimensional and complex, and it covers socio-demographic characteristics, health conditions, and the use of health services. The explanation about the disease and its treatment to the patient and the expansion of the universal access to pharmaceutical care can minimize the risks of underuse.

https://doi.org/10.11606/s1518-8787.2018052000622 36 downloads

15.

Cost-related underuse of medications in older adults: ELSI-Brazil
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Loyola Filho, Antônio Ignácio de

; Firmo, Josélia Oliveira Araújo

; Mambrini, Juliana Vaz de Melo

; Peixoto, Sérgio Viana

; Souza Junior, Paulo Roberto Borges de

; Andrade, Fabíola Bof de

; Lima-Costa, Maria Fernanda

; Acúrcio, Francisco de Assis

Revista de Saúde Pública

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2018, Volume 52 Suppl. 2 elocation 8s

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.11606/s1518-8787.2018052000622 1563 downloads

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