RESUMO Especula-se que a pandemia pelo novo coronavírus SARS-CoV-2 (COVID-19) tenha agravado a prevalência de sobrepeso e obesidade em cães e gatos. O presente artigo teve como objetivo realizar um levantamento de sobrepeso/obesidade, o qual avaliou os registros de 1001 casos consecutivos atendidos no Hospital de Clínicas Veterinárias da Universidade Federal do Rio Grande do Sul, durante a reabertura da instituição ao público, após meses fechada devido à pandemia. Observou-se uma prevalência de sobrepeso ou de obesidade de 36,63%, sendo a frequência ligeiramente maior em gatos (38,37%) do que em cães (35,76%) e não sendo documentado aumento local na prevalência. Castrações (cães e gatos), idade > 10 anos (cães) e sexo masculino (gatos) foram associadas ao sobrepeso/obesidade. Em cerca de 30% das fichas não havia peso registrado, e em cerca de 50% não havia registro de ECC. Essas falhas foram mais frequentes em fichas de felinos e demandam medidas para aumentar o registro desses dados pelas equipes clínicas.

ABSTRACT Anecdotal reports suggest that the new coronavirus SARS-CoV-2 pandemic (COVID-19) has increased overweight and obesity prevalence in dogs and cats. The present report aimed to perform a retrospective overweight/obesity survey on 1,001 consecutive cases attended in the Veterinary Clinics Hospital from the Federal University of Rio Grande do Sul during its reopening to the public after months closed due to the pandemic. The overall overweight or obesity observed prevalence was 36.63%; being the frequency in cats slightly greater (38.37%) than compared to dogs (35.76%). No local increase in these frequencies was detected. Neutering (dogs and cats), age greater than 10 years (dogs), and male gender (cats) were factors associated with overweight/obesity. Bodyweight was not registered in about 30% of the medical records, while the body condition score was not registered in about 50% of the cases. These flaws were more often observed in feline medical records and efforts to improve these data records are warranted.

Abstract Being a food product that contains perishable ingredients and involves a significant degree of manual handling during preparation, sushi is regarded as a potentially hazardous food, which may lead to foodborne disease outbreaks. In Portugal, consumption of takeaway sushi meals has strongly increased throughout the past few years; however, there is limited information regarding its compliance with food quality standards. Under this context, the present study aimed to evaluate the microbiological quality and safety of take-away ready-to-eat sushi meals in Lisbon, Portugal. Sixty-two samples were collected from different origins (restaurant and hypermarket), and each sample was tested for aerobic mesophilic microorganisms, Enterobacteriaceae, Escherichia coli, positive coagulase Staphylococci, presumptive Bacillus cereus count, as for detection of pathogenic microorganisms, such as Salmonella spp., Listeria monocytogenes and Vibrio parahaemolyticus, V. cholerae and V. vulnificus. Results revealed that 48.4% (30/62) were deemed unsatisfactory, 35.5% (22/62) were classified as borderline and only 16.1% (10/62) were considered satisfactory. Even though we did not detect the incidence of potentially pathogenic microorganisms in sushi, the presence of B. cereus and coagulase-positive Staphylococci was detected at unsatisfactory levels. Furthermore, significant differences between the place of origin (restaurant vs. hypermarket) and type of fish were also observed. Overall, the high number of samples classified with a level of microbiological quality “unsatisfactory” and “borderline” highlights the need to review good hygiene practices, as well as the quality of the raw materials used, to obtain a final product with a satisfactory quality and safety level.

Resumo Por ser um género alimentício que contém ingredientes perecíveis e envolve um grau significativo de manipulação manual durante a sua preparação, o sushi é considerado um alimento potencialmente perigoso, que pode causar surtos de doença de origem alimentar. Em Portugal, o consumo de refeições de sushi prontas a consumir tem aumentado ao longo dos últimos anos. No entanto, a informação sobre o cumprimento das normas de qualidade alimentar é limitada. Neste contexto, o presente estudo teve como objetivo avaliar a qualidade e a segurança microbiológica de refeições de sushi prontas para consumo em take-way, na região de Lisboa, Portugal. Foram colhidas 62 amostras de diferentes origens (restaurante e hipermercado), e em cada amostra foi efetuada a contagem de microrganismos aeróbios mesófilos, Enterobacteriaceae, Escherichia coli, estafilococos coagulase positiva, Bacillus cereus presuntivos, e deteção de microrganismos patogénicos, tais como: Salmonella spp., Listeria monocytogenes, Vibrio parahaemolyticus, Vibrio cholerae e Vibrio vulnificus. Os resultados revelaram que 48,4% (30/62) das amostras foram consideradas insatisfatórias, 35,5% (22/62) foram classificadas como “borderline” e apenas 16,1% (10/62) foram consideradas como satisfatórias. Embora não tenham sido detetados microrganismos potencialmente patogénicos nas amostras de sushi, a presença de B. cereus e estafilococos coagulase positivos foram detetados em níveis insatisfatórios. Além disso, também foram observadas diferenças significativas entre o local de origem (restaurante vs. hipermercado) e tipo de peixe.No geral, o elevado número de amostras classificadas com um nível de qualidade microbiológica insatisfatória e “borderline” evidência a necessidade de revisão das boas práticas de higiene, bem como da qualidade das matérias-primas utilizadas, para obter um produto final com qualidade e segurança satisfatória.

RESUMO O objetivo do presente estudo foi avaliar os efeitos da substituição da silagem de milho por cana-de-açúcar na dieta de cabras Saanen em lactação e determinar a ingestão e a digestibilidade de nutrientes, o comportamento ingestivo, a produção e a composição do leite. As dietas experimentais foram compostas de níveis crescentes (0, 33, 66 e 100%) de substituição na matéria seca (MS). Doze cabras Saanen multíparas, com peso corporal médio de 45,2kg, produção média de leite de 3,0kg dia-1, foram distribuídas em delineamento experimental triplo quadrado latino (4 × 4). A ingestão de matéria seca (IMS) e de outros nutrientes foi estimada por meio da diferença entre o total de nutrientes nos alimentos oferecidos e o total nas sobras. A IMS, a proteína bruta, a fibra em detergente neutro (FDN) e os nutrientes digestíveis totais não foram influenciados, mas a digestibilidade aparente da MS e da FDN diminuiu. A produção de leite não foi influenciada pelos níveis de substituição, mas a produção de leite corrigida para 3,5% de gordura diminuiu. A cana-de-açúcar representa uma alternativa alimentar para cabras com produção média de leite em períodos críticos de forragem, pois não altera o consumo de MS e a produção de leite, mesmo diminuindo a digestibilidade aparente de alguns nutrientes e influenciando o comportamento ingestivo dos animais.

ABSTRACT The aim of the present study was to assess the effects of replacing corn silage with sugarcane in the diet of lactating Saanen goats and to determine their intake and digestibility of nutrients, ingestive behavior, milk yield and composition. The experimental diets were composed of increasing levels (0, 33, 66 and 100%) of substitution in dry matter (DM). Twelve multiparous Saanen goats, with an average body weight of 45.2kg, average milk yield of 3.0kg day-1, distributed in a triple latin square experimental design (4 × 4) were used. The dry matter intake (DMI) and other nutrients were estimated through the difference between the total nutrient in the food offered and its total in the leftovers. The DMI, crude protein, neutral detergent fiber (NDF) and total digestible nutrients were not influenced, but the apparent digestibility of DM and NDF decreased. Feeding time and feeding efficiency were not influenced, the rumination and total chewing times increased, and the leisure time decreased, both linearly. Milk yield was not influenced by substitution levels, but corrected milk yield to 3.5% fat decreased. Sugar cane represents a dietary alternative for goats with medium milk yield in critical periods of forage, since it does not change the consumption of DM and milk yield, even with the apparent declining digestibility of some nutrients, influencing the ingestive behavior of the animals.

RESUMO: A interferência entre sistemas de raízes ocorre abaixo da superfície do solo muito antes que a competição entre plantas se expresse na parte aérea. Entre os recursos de competição, os nutrientes são destacados pela limitação nos solos, tornando necessária a suplementação, especialmente do fósforo (P). Dessa forma, objetivou-se neste estudo estimar, a partir da semeadura, características visuais nos sistemas de raízes do feijoeiro (Phaseolus vulgaris) de variedade Carioca, em competição com braquiária (Urochloa brizantha), considerando-se ainda a aplicação de fósforo P2O5. Utilizou-se esquema fatorial com seis tratamentos, resultantes da combinação de três esquemas de cultivo (feijoeiro em cultivo isolado ou em competição com uma ou duas plantas de braquiária) e aplicação ou não de adubação fosfatada (90 kg ha-1 de P2O5), conduzido em delineamento inteiramente casualizado em casa de vegetação com ambiente controlado, com quatro repetições. Após a semeadura das espécies, os resultados da visualização foram tomados a cada 12 horas, por meio do registro fotográfico do desenvolvimento do sistema radicular em rizotrons. Foi observado maior crescimento e área total do sistema radicular quando em monocultivo e em competição com uma planta de braquiária em presença de adubação fosfatada. Em contrapartida, o feijão em recipiente com duas plantas de braquiária investiu recursos em maior comprimento e área total do sistema radicular em ausência do fertilizante. Pode-se afirmar a existência de indícios da interferência a partir de três dias após a semeadura das espécies.

ABSTRACT: Interference between root systems occurs below the ground surface long before the competition between plants is expressed in the shoot. Nutrients stand out for being limited in the soil, requiring supplementation, especially phosphorus. Thus, this study aimed at estimating, from sowing, visual characteristics in the root systems of bean plants from the Carioca variety (Phaseolus vulgaris) when in competition with brachiaria (Urochloa brizantha), considering also the application of phosphorus P2O5. A factorial design with six treatments was used resulting from the combination of three cultivation schemes (beans in isolated crops or in competition with one or two brachiaria plants), and the application or not of phosphate fertilizer (90 kg ha-1 of P2O5), in a completely randomized design with four replications. After the sowing of the species, the visualization results were taken at every 12 hours, by means of the photographic record of the development of the root system in rhizotrons. Greater growth and greater total area of the root system were observed in bean monocultures and when competing with one brachiaria plant in the presence of phosphate fertilization. In contrast, the bean plant in the container with two brachiaria plants invested its resources in greater length and total area of the root system when in the absence of the fertilizer. It can be stated that there is evidence of interference starting three days after the sowing of the species.

Maternal smoking is a risk factor for progeny obesity. We have previously shown, in a rat model of neonatal tobacco smoke exposure, a mild increase in food intake and a considerable increase in visceral adiposity in the adult offspring. Males also had secondary hyperthyroidism, while females had only higher T4. Since brown adipose tissue (BAT) hypofunction is related to obesity, here we tested the hypothesis that higher levels of thyroid hormones are not functional in BAT, suggesting a lower metabolic rate. We evaluated autonomic nerve activity in BAT and its function in adult rats that were exposed to tobacco smoke during lactation. At birth, litters were adjusted to 3 male and 3 female pups/litter. From postnatal day (PND) 3 to 21, Wistar lactating rats and their pups were divided into SE group, smoke-exposed in a cigarette smoking machine (4 times/day) and C group, exposed to filtered air. Offspring were sacrificed at PND180. Adult SE rats of both genders had lower interscapular BAT autonomic nervous system activity, with higher BAT mass but no change in morphology. BAT UCP1 and CPT1a protein levels were decreased in the SE groups of both genders. Male SE rats had lower β3-AR, TRα1, and TRβ1 expression while females showed lower PGC1α expression. BAT Dio2 mRNA and hypothalamic POMC and MC4R levels were similar between groups. Hypothalamic pAMPK level was higher in SE males and lower in SE females. Thus, neonatal cigarette smoke exposure induces lower BAT thermogenic capacity, which can be obesogenic at adulthood.

ABSTRACT The objective of this study was to evaluate the testicular echotexture of Nelore bulls and to correlate it with spermatogenic parameters. Forty one bulls aged 3-5 years, with body weight of 510.2 ± 21.61 kg were selected for the study. The animals were subjected to ultrasonography, using a portable device coupled with a linear transducer at a frequency of 7.5 MHz. Immediately afterwards they were castrated and the testicular tissue was treated with Karnovsky fixative. Next, the fragments were embedded in glycol methacrylate and stained with toluidine blue-1% sodium borate solution. The average pixel intensity in the testicular parenchyma was determined for 1,600-mm2 surface sections. The right and left testicles and the area between the two testicles resulted in average pixel intensities of 105.22 ± 13.58, 107.95 ± 12.82, and 106.59 ± 11.43, respectively. The efficiency coefficient of spermatogonial mitosis was 19.74; meiotic index was 2.63, and overall yield of spermatogenesis was 52.0. No correlation (P>0.05) was observed between testicular echotexture and any of the measured morphometric parameters. These results suggest that testicular echotexture was not a viable method for the indirect analysis of cellular population in the seminiferous epithelium, intrinsic spermatogenic performance, or the Sertoli cell index in Nelore bulls.

RESUMEN El objetivo de este estudio fue evaluar la ecotextura testicular de los toros Nelore y correlacionarlo con los parámetros de espermatogénesis. Para este propósito, 41 toros de edades de 3-5 años y con pesos corporales en el intervalo de 510,2 ± 21,61 kg fueron seleccionados para el estudio. Los toros se sometieron a ecografía, utilizando un dispositivo portátil junto con un transductor lineal a una frecuencia de 7,5 MHz. Inmediatamente después, los animales fueron castrados, y el tejido testicular se trató con Karnovsky fijador. Los fragmentos testiculares se incluyeron en metacrilato de glicol y se tiñeron con una solución de borato de sodio-azul de toluidina al 1%. La intensidad media de pixeles en el parénquima testicular se determinó para secciones de superficie de 1.600 mm2. Los El área entre los dos testículos derecho e izquierdo dieron lugar a la intensidad media de los pixeles de 105,22 ± 13,58, 107,95 ± 12,82 y 106,59 ± 11,43, respectivamente. El coeficiente de eficiencia de la mitosis espermatogonial fue 19,74; Índice meiótico fue 2,63, y el rendimiento global del espermatogénesis alcanzó el 52,0. No se observó correlación (P>0,05) entre ecotextura testicular y cualquiera de los parámetros morfométricos medidos. Estos resultados sugieren que ecotextura testicular no es un método viable para el análisis indirecto de la población celular en el epitelio seminífero, el rendimiento de la espermatogénesis intrínseco, o el índice de células de Sertoli en toros Nelore.

A number of infectious diseases amongst travelers and the immigrant populations are a major public health concern. Some have a long incubation period or remain asymptomatic or paucisymptomatic for many years before leading to significant clinical manifestations and/or complications. HIV, hepatitis B and C, tuberculosis or latent syphilis are among the most significant persistent diseases in migrants. Schistosomiasis and strongyloidiasis, for instance, are persistent helminthic infections that may cause significant morbidity, particularly in patients co-infected with HIV, hepatitis B and C. Chagas disease, which was initially confined to Latin America, must also now be considered in immigrants from endemic countries. Visceral leishmaniasis and malaria are other examples of parasitic diseases that must be taken into account by physicians treating incarcerated migrants. The focus of this review article is on the risk of neglected tropical diseases in particularly vulnerable correctional populations and on the risk of infectious diseases that commonly affect migrants but which are often underestimated.

Son muchas las enfermedades en viajeros y poblaciones inmigrantes que suponen un problema de salud pública de primer orden. Algunas tienen un periodo largo de incubación o son asintomáticas o paucisintomático durante muchos años antes de provocar manifestaciones clínicas significativas y/o complicaciones. La infección por VIH, hepatitis B y C, la tuberculosis o la sífilis latente se encuentran entre las enfermedades persistentes más relevantes en población emigrante. La esquistosomiasis y la estrongiloidiasis, por ejemplo, son infecciones helmínticas persistentes que pueden causar importante morbilidad, especialmente en pacientes coinfectados por VIH, hepatitis B y C. La enfermedad de Chagas, que inicialmente se creyó limitada a América Latina, ahora también tiene que ser considerada en los inmigrantes de países endémicos. La leishmaniasis visceral y la malaria son otros ejemplos de enfermedades parasitarias que tienen que tenerse en cuenta cuando se trata con inmigrantes encarcelados. El objetivo de este artículo es revisar el riesgo de las enfermedades tropicales desatendidas en especial dada la vulnerabilidad de la población penitenciaria y el riesgo de las enfermedades infecciosas que normalmente afectan a emigrantes pero que a menudo son infraestimadas.

Objetivou-se com este estudo investigar a ocorrência de Mycoplasma spp., Mycoplasma galissepticum (MG) e Mycoplasma synoviae (MS) em psitacídeos de cativeiro localizado no estado de Pernambuco, Brasil. Foram estudadas 85 aves provenientes do Parque Estadual Dois Irmãos, localizado no estado do Pernambuco, Brasil. De cada psitacídeo analisado foram obtidas três amostras por meio de swabs da cloaca, palato e conjuntiva totalizando 255 amostras. As amostras coletadas foram submetidas à extração de DNA e à reação em cadeia da polimerase (PCR), sendo as positivas submetidas ao isolamento em ágar Frey. O DNA de Mycoplasma spp. foi detectado em 16,47% (14/85) dos psitacídeos estudados. Das 255 amostras analisadas, 6,66% (17/255) foram positivas para a presença de Mycoplasma spp., sendo 41,18% (7/17) provenientes da conjuntiva, 35,29% (6/17) do palato e 23,53% (4/17) da cloaca. Nenhuma amostra foi positiva para MG ou MS na PCR. Os resultados obtidos permitem confirmar a presença do DNA de Mycoplasma spp. em conjuntiva, palato e cloaca nas aves estudadas. Foram detectadas colônias semelhantes a membros da classe Mollicutes em 17,64% das amostras (3/17). Esse é o primeiro relato da presença de Mycoplasma spp. em psitacídeos de cativeiro no Nordeste do Brasil.

The aim of this study was to investigate the occurrence of Mycoplasma spp., Mycoplasma galissepticum (MG) and Mycoplasma synoviae (MS) in captive psittacines. Eighty-five wild birds from Parque Estadual Dois Irmãos, Pernambuco state, northeastern Brazil, were used. From each psittacid analyzed three samples were obtained through cloaca, palate and conjunctiva swabs, totaling 255 samples. Samples collected were submitted to DNA extraction and Polimerase Chain Reaction (PCR). Mycoplasma spp. DNA was detected in 16.47% (14/85) of psittacines studied. From 255 samples, 6.66% (17/255) were positive for Mycoplasma spp.: 41.18% (7/17) of positivity in conjunctiva, 35.29% (6/17) in palate and 23.53% (4/17) in cloaca. There was no positive sample for MG or MS in PCR. Similar colonies were found for members of the Mollicutes Class in 17.64% of the samples (3/17). The results confirmed Mycoplasma spp. DNA in conjunctiva, palate and cloaca from the wild birds analyzed. This is the first record of Mycoplasma spp. in captive psittacines from northeastern Brazil.

The parasympathetic nervous system is important for β-cell secretion and mass regulation. Here, we characterized involvement of the vagus nerve in pancreatic β-cell morphofunctional regulation and body nutrient homeostasis in 90-day-old monosodium glutamate (MSG)-obese rats. Male newborn Wistar rats received MSG (4 g/kg body weight) or saline [control (CTL) group] during the first 5 days of life. At 30 days of age, both groups of rats were submitted to sham-surgery (CTL and MSG groups) or subdiaphragmatic vagotomy (Cvag and Mvag groups). The 90-day-old MSG rats presented obesity, hyperinsulinemia, insulin resistance, and hypertriglyceridemia. Their pancreatic islets hypersecreted insulin in response to glucose but did not increase insulin release upon carbachol (Cch) stimulus, despite a higher intracellular Ca2+ mobilization. Furthermore, while the pancreas weight was 34% lower in MSG rats, no alteration in islet and β-cell mass was observed. However, in the MSG pancreas, increases of 51% and 55% were observed in the total islet and β-cell area/pancreas section, respectively. Also, the β-cell number per β-cell area was 19% higher in MSG rat pancreas than in CTL pancreas. Vagotomy prevented obesity, reducing 25% of body fat stores and ameliorated glucose homeostasis in Mvag rats. Mvag islets demonstrated partially reduced insulin secretion in response to 11.1 mM glucose and presented normalization of Cch-induced Ca2+ mobilization and insulin release. All morphometric parameters were similar among Mvag and CTL rat pancreases. Therefore, the higher insulin release in MSG rats was associated with greater β-cell/islet numbers and not due to hypertrophy. Vagotomy improved whole body nutrient homeostasis and endocrine pancreatic morphofunction in Mvag rats.

The objective of this prospective study was to determine the plasma levels of nitric oxide (NO) in women with chronic pelvic pain secondary to endometriosis (n=24) and abdominal myofascial pain syndrome (n=16). NO levels were measured in plasma collected before and 1 month after treatment. Pretreatment NO levels (μM) were lower in healthy volunteers (47.0±12.7) than in women with myofascial pain (64.2±5.0, P=0.01) or endometriosis (99.5±12.9, P<0.0001). After treatment, plasma NO levels were reduced only in the endometriosis group (99.5±12.9 vs 61.6±5.9, P=0.002). A correlation between reduction of pain intensity and reduction of NO level was observed in the endometriosis group [correlation = 0.67 (95%CI = 0.35 to 0.85), P<0.0001]. Reduction of NO levels was associated with an increase of pain threshold in this group [correlation = -0.53 (-0.78 to -0.14), P<0.0001]. NO levels appeared elevated in women with chronic pelvic pain diagnosed as secondary to endometriosis, and were directly associated with reduction in pain intensity and increase in pain threshold after treatment. Further studies are needed to investigate the role of NO in the pathophysiology of pain in women with endometriosis and its eventual association with central sensitization.

Em vista da grande quantidade de agroquímicos aplicados na agricultura, principalmente os herbicidas, cresce a preocupação com a possível contaminação do ambiente com esses produtos, inclusive em corpos dâ€(tm)água. Diante do exposto, o objetivo deste trabalho foi detectar e quantificar herbicidas, por meio de análise multirresíduo, em amostras de água coletadas em poços semiartesianos e nascentes, em uma área rural da cidade de Jaboticabal (SP). Foram coletadas amostras em 32 poços e 13 nascentes, em três épocas: outubro de 2010, fevereiro e maio de 2011. Foram coletadas, adicionalmente, amostras em uma residência na área urbana. Foi realizada análise por cromatografia líquida de alta eficiência acoplada à espectrometria de massas e foram avaliados os herbicidas ametryn, amicarbazone, clomazone, diclosulan, diuron, hexazinone, imazapic, imazapyr, isoxaflutole, S-metolachlor, sulfentrazone, sulfometuron-methyl e tebuthiuron. Em poços semiartesianos foram encontrados mais herbicidas em relação às nascentes. Entre os herbicidas avaliados, hexazinone, imazapyr e sulfentrazone foram detectados em valores quantificáveis de acordo com o método analítico, ao passo que o clomazone foi o herbicida mais frequente, sendo detectado em mais de 60% das amostras. Foram detectados também os herbicidas ametryn, amicarbazone e diuron. Em nenhuma das amostras foram detectados os herbicidas diclosulan, imazapic, isoxaflutole, S-metolachlor, sulfometuron-methyl e tebuthiuron. A utilização incorreta desses produtos, sem um prévio conhecimento do comportamento deles no solo, faz com que haja contaminação de águas subterrâneas e nascentes, tornando importante o monitoramento constante desse recurso.

Due to the large amount of pesticides applied in agriculture, mainly herbicides, there is a growing concern about a possible environmental contamination with these products, including water bodies. Given the above, the aim of the present work was to detect and quantify herbicides through multiresidue analysis in water samples collected in semi-artesian wells and springs in a rural area of the city of Jaboticabal (SP). Samples were collected from 32 wells and 13 water springs, in three different seasons: October 2010, February 2011 and May 2011. Additionally, samples at a residence in the urban area were also collected. Analysis using high performance liquid chromatography coupled to mass spectrometry was performed and herbicides ametryn, amicarbazone, clomazone, diclosulan, diuron, hexazinone, imazapic, imazapyr, isoxaflutole, S-metolachlor, sulfentrazone, sulfometuron-methyl, and tebuthiuron were evaluated. In semi-artesian wells, an incresed quantity of herbicides was found in comparison with the water springs. Among the tested herbicides, hexazinone, imazapyr and sulfentrazone were detected in measurable amounts in accordance with the analytical method applied, while clomazone was the most common herbicide being detected in more than 60% of the samples. Ametryn, diuron and amicarbazone herbicides were also detected. Diclosulan, imazapic, isoxaflutole, S-metolachlor, sulfometuron-methyl, and tebuthiuron were not detected in any sample. Inappropriate use of these products without prior knowledge of the behavior of the soil can lead to groundwaters and water springs contamination, thus an ongoing monitoring of this resource becomes very important.

The present work was conducted to evaluate the use of cassava bagasse (BM) in rations with enzyme complex (CE) as supplement for broilers in two phases. Two experiments were conducted to assess the weight gain (GP), feed intake (CR) and feed conversion (CA) in the phases 1-21 and 22-40 days of age, of broilers receiving diets containing 0 and 20 % of BM and, with or without CE. The experiments were conducted in a completely randomized block design with 8 replicates and 20 broiler per experimental unit using a 2x2 factorial arrangement (0 and 20 % of BM, with or without CE). There was no interaction between treatments, inclusion of BM and CE. The inclusion of BM decreased CR and GP in both phases. Whereas the inclusion of CE improved GP and CA at the initial stage did not affect the performance at 22-40 days of age, the cost of feed per kilogram of weight gain was lower and the rates of economic efficiency and cost for both phases was better in animals subjected to diets with 0 % of cassava bagasse inclusion with the enzymatic complex.

Objetivou-se avaliar a utilização de bagaço de mandioca (BM) em rações suplementadas com complexo enzimático (CE) para frangos de corte em duas fases. Foram avaliados o ganho de peso (GP), consumo de ração (CR) e conversão alimentar (CA) de frangos de corte nas fases de 1 a 21 e 22 a 40 dias, alimentados com rações contendo 0 e 20 % de inclusão de BM com e sem adição de CE. Os experimentos foram realizados num delineamento experimental em blocos casualizados com 4 tratamentos utilizando um arranjo fatorial 2x2 (0 e 20 % de BM, com e sem CE) com 8 repetições e 20 aves por unidade experimental. Não foi observada interação entre os tratamentos, inclusão de BM e CE. A inclusão do BM diminuiu o CR e o GP em ambas as fases de criação. Enquanto que a inclusão do CE melhorou o GP e a CA das aves na fase inicial, não afetando o desempenho na fase de 22 a 40 dias de idade, já menor custo de ração por quilograma de ganho de peso e os melhores índices de eficiência econômica e de custo para ambas as fases foi encontrado para os animais que foram submetidos a rações com 0 % de inclusão de bagaco de mandioca com adição de complexo enzimatico.

Avaliou-se o efeito da utilização de níveis crescente de minerais orgânicos sobre a qualidade externa e interna de ovos e a resistência de ossos da tíbia de poedeiras semi-pesadas. Foram utilizadas 256 aves, com idade inicial de 30 semanas, distribuídas em 64 gaiolas perfazendo quatro aves por gaiola em delineamento inteiramente ao acaso. Os tratamentos consistiram da inclusão de minerais inorgânicos ou níveis crescentes de orgânicos na dieta basal das poedeiras, resultando em quatro tratamentos. Foram avaliados peso do ovo (g), gravidade específica, peso (g) e espessura (mm) da casca, altura do albúmen (mm), unidade Haugh, peso da gema (g) e do albúmen (g) e resistência óssea (kg). Houve diferença significativa (P>0,05) entre as médias para peso do ovo e do albúmen. O fornecimento de dietas suplementadas com minerais orgânicos, em nível intermediário resultou na produção de ovos mais pesados, com maior peso de albúmen, e na manutenção das demais características de qualidade externa e interna dos ovos e de resistência óssea.

The effect of increasing levels of organic minerals on the external and internal quality of eggs and tibia bone strength of brown-egg laying hens was studied. A total of 256, 30-week old was equally distributed in 64 cages in a total of four birds per cage in a completely randomized experimental design. The dietary treatments consisted of inorganic or increasing levels of organic minerals in the diets of laying hens, resulting in four treatments. Egg weight (g), specific gravity, shell weight and thickness (mm), albumen height (mm), Haugh unit, yolk weight and albumen (g) and bone strength (kg) were evaluated. A significant difference (P<0.05) among treatment means for egg weight and albumen was found. Intermediate inclusion levels of organic minerals resulted in the production of heavier eggs, with higher albumen weight and maintenance of the other characteristics of internal and external quality of eggs and bone strength.

Endometriosis is a complex and multifactorial disease. Chromosomal imbalance screening in endometriotic tissue can be used to detect hot-spot regions in the search for a possible genetic marker for endometriosis. The objective of the present study was to detect chromosomal imbalances by comparative genomic hybridization (CGH) in ectopic tissue samples from ovarian endometriomas and eutopic tissue from the same patients. We evaluated 10 ovarian endometriotic tissues and 10 eutopic endometrial tissues by metaphase CGH. CGH was prepared with normal and test DNA enzymatically digested, ligated to adaptors and amplified by PCR. A second PCR was performed for DNA labeling. Equal amounts of both normal and test-labeled DNA were hybridized in human normal metaphases. The Isis FISH Imaging System V 5.0 software was used for chromosome analysis. In both eutopic and ectopic groups, 4/10 samples presented chromosomal alterations, mainly chromosomal gains. CGH identified 11q12.3-q13.1, 17p11.1-p12, 17q25.3-qter, and 19p as critical regions. Genomic imbalances in 11q, 17p, 17q, and 19p were detected in normal eutopic and/or ectopic endometrium from women with ovarian endometriosis. These regions contain genes such as POLR2G, MXRA7 and UBA52 involved in biological processes that may lead to the establishment and maintenance of endometriotic implants. This genomic imbalance may affect genes in which dysregulation impacts both eutopic and ectopic endometrium.

Foram avaliados os efeitos de duas fontes de gordura, Lac100® (Yakult), fonte de ácido graxo ômega-6, e linhaça em grão (Linum usitatissimum) (LIN), fonte de ácido graxo ômega-3, sobre a digestibilidade aparente da matéria seca (DMS), proteína bruta (DPB), fibra em detergente neutro (DFDN), fibra em detergente ácido (DFDA) e extrato etéreo (DEE), e concentrações sanguíneas de HDL, LDL, VLDL, colesterol, triglicerídeos, glicose, cálcio, fósforo, N-ureico e progesterona. Foram utilizadas 10 vacas da raça Holandesa em lactação, distribuídas nos dois tratamentos, em um delineamento estatístico inteiramente casualizado. Os animais alimentados com Lac100® apresentaram maiores valores de DFDN (57,44% vs. 50,80%) e DEE (77,70% vs. 72,18%) quando comparados aos alimentados com LIN. Os animais alimentados com Lac100® apresentaram maiores concentrações de LDL (111,00 vs. 45,46mg/dL), HDL (82,27 vs. 64,93mg/dL) e colesterol total (203,60 vs. 116,13mg/dL) e menor concentração de N-ureico (13,22 vs. 15,19mg/dL) em relação aos animais alimentados com linhaça em grão. As concentrações sanguíneas de glicose, cálcio, fósforo e progesterona não foram alteradas. Os resultados sugerem que a fonte de gordura na dieta modifica a DFDN e a DEE e, as concentrações sanguíneas de LDL, HDL e colesterol total em vacas em lactação.

The effect of two sources of fat, calcium salts of soybean oil, Lac100® (Yakult), a source of omega-6 fatty acids or whole flaxseed (Linum usitatissiumum), a source of omega-3 fatty acids were evaluated to determine the apparent digestibility of dry matter (DDM), crude protein (DCP), neutral detergent fiber (DNDF), acid detergent fiber (DADF), and ether extract (DEE); and blood concentrations of HDL, LDL, VLDL, cholesterol, triglycerides, glucose, calcium, phosphorus, N-urea, and progesterone. Ten lactating Holstein cows were allocated in two treatments and a completely randomized design was used. Cows fed Lac100® had higher values of DNDF (57.44% vs. 50.80%) and DEE (77.70% vs. 72.18%) compared to those fed flaxseed. Cows fed Lac100® had higher blood concentrations of LDL (111.00 vs. 45.46mg/dL), HDL (82.27 vs. 64.93mg/dL), and total cholesterol (203.60 vs. 116.13mg/dL) and lower N-urea (13.22 vs. 15.19mg/dL) compared to those fed flaxseed. Blood concentrations of glucose, calcium, phosphorus, and progesterone were similar between the treatments. These results suggest that dietary fat modifies DNDF and DEE and blood concentrations of LDL, HDL, VLDL, and total cholesterol in lactating dairy cows.