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ABSTRACT Introduction: HIV/AIDS is considered one of the great cases of public health, but it is seen that patients who use antiretroviral therapy (ART) and practice strength training promote a promotion of their health. Objectives: Assess the impact of strength and resistance training on cytokines and body composition in people living with HIV/AIDS. Methods: Randomized clinical trial, the sample consisted of 12 patients, 7 from the Strength Group (GF) and 5 from Group 2, Muscular Resistance (MGR). We compared the levels of IL-2, IL-4, IL-6, IL-10 and TNF-α cytokines and body composition in the first and last sessions. The patients completed 36 strength and resistance training sessions over 12 weeks. Results: After 36 sessions of GRM resistance training, there was a significant increase from 4,734 pg/mL to 5,050 pg/mL of IL-10 (p=0.002). Regarding the GFR, no significant results were found. For body composition, there were significant differences in GFR due to the increase in lean mass of the arms from 6,441g to 7,014g (p=0.04), legs from 16,379g to 17,281g (p=0.02) and whole body of 45,640g to 47,343g (p=0.01). In G2 there was a significant decrease in the percentage of fat in the arms from 23,160% to 20,750% (p = 0.04). To assess quality of life, the WHOQOL-HIV-Bref questionnaire was used, where significant improvement was found in all domains, except for the level of independence domain. Conclusion: We conclude that muscular resistance training is effective in increasing IL-10 and decreasing the percentage of fat in the arms, whereas strength training increases lean mass in arms, legs, and the whole body. Level of Evidence I; Randomized Clinical Trial.

RESUMEN Introducción: El VIH/SIDA es considerado uno de los grandes casos de salud pública, sin embargo, está comprobado que pacientes que hacen uso de la terapia antirretroviral (TARV) y practican entrenamiento de fuerza provoca una promoción de su salud. Objetivos: Evaluar el impacto del entrenamiento de fuerza en la resistencia a las citoquinas y en la composición corporal de las personas que viven con VIH/SIDA. Métodos: Ensayo clínico aleatorizado, la muestra estuvo compuesta por 12 pacientes, siete del Grupo de Fuerza (TFG) y cinco del Grupo de Resistencia Muscular (GRM). Se compararon los niveles de las citocinas IL-2, IL-4, IL-6, IL-10 y TNF-α y la composición corporal en la primera y la última sesión. Los pacientes completaron 36 sesiones de entrenamiento de fuerza y resistencia durante 12 semanas. Resultados: Tras 36 sesiones de entrenamiento de resistencia GRM, se produjo un aumento significativo de 4.734 pg/mL a 5.050 pg/mL de IL-10 (p=0,002). En cuanto a la TFG, no se encontraron resultados significativos. En cuanto a la composición corporal, hubo diferencias significativas en la TFG debido al aumento de la masa magra en brazos de 6.441g a 7.014g (p=0,04), piernas de 16.379g a 17.281g (p=0,02) y cuerpo entero de 45.640g a 47.343g (p=0,01). En el GRM hubo una disminución significativa del porcentaje de grasa en los brazos de 23.160% a 20.750% (p = 0,04). Para la evaluación de la calidad de vida se utilizó el cuestionario WHOQOL-HIV-Bref, donde se encontró una mejoría significativa en todos los dominios, excepto en el dominio nivel de independencia. Conclusión: Concluimos que el entrenamiento de resistencia muscular es eficaz para aumentar la IL-10 y disminuir el porcentaje de grasa en los brazos, mientras que el entrenamiento de fuerza aumenta la masa magra total. Nivel de Evidencia I; Ensayo clínico aleatorizado.

RESUMO Introdução: O HIV/AIDS é considerado um dos grandes casos de saúde pública, porém verifica-se que pacientes que fazem uso de terapia antirretroviral (TARV) e praticam treinamento de força provocam uma promoção de sua saúde. Objetivos: Avaliar o impacto do treinamento de força sobre a resistência nas citocinas e a composição corporal de pessoas vivendo com HIV/AIDS. Métodos: Ensaio clínico randomizado, a amostra foi composta por 12 pacientes, sendo sete do Grupo Força (TFG) e cinco do Grupo Resistência Muscular (GRM). Comparou-se os níveis das citocinas IL-2, IL-4, IL-6, IL-10 e TNF-α e a composição corporal na primeira e na última sessão. Os pacientes completaram 36 sessões de treinamento de força e resistência ao longo de 12 semanas. Resultados: Após 36 sessões de treinamento resistido GRM, houve um aumento significativo de 4.734 pg/mL para 5.050 pg/mL de IL-10 (p=0,002). Em relação à TFG, não foram encontrados resultados significativos. Para composição corporal, houve diferenças significativas na TFG devido ao aumento da massa magra dos braços de 6.441g para 7.014g (p=0,04), pernas de 16.379g para 17.281g (p=0,02) e corpo inteiro de 45.640g para 47.343g (p=0,01). No GRM houve diminuição significativa do percentual de gordura nos braços de 23.160% para 20.750% (p = 0,04). Para avaliação da qualidade de vida foi utilizado o questionário WHOQOL-HIV-Bref, onde foi encontrada uma melhora significativa em todos os domínios, exceto no domínio nível de independência. Conclusão: Conclui-se que o treinamento de resistência muscular é eficaz em aumentar a IL-10 e diminuir o percentual de gordura nos braços, enquanto o treinamento de força aumenta a massa magra geral. Nível de Evidência I; Ensaio Clínico Randomizado.

https://doi.org/10.1590/1517-8692202430012022_0123i

Videolaryngoscopy in anesthesia and perioperative medicine: innovations, challenges, and best practices medicine innovations challenges
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Quintao, Vinícius Caldeira

; Carvalho, Vanessa Henriques

; Costa, Luiz Guilherme Villares da

; Germano-Filho, Paulo Alipio

; Nascimento, José Carlos R.

; Lima, Rodrigo Moreira e

; Nunes, Rogean Rodrigues

; Brandão, Antônio Carlos

; Schmidt, André P.

Brazilian Journal of Anesthesiology

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2023, Volume 73 Nº 5 Páginas 525 - 528

Resumo: En | Texto: En | PDF: En

https://doi.org/10.1016/j.bjane.2023.08.003

Sustainable nitrogen efficiency in wheat by the dose and mode of supply
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Trautmann, Ana P. B.

; Silva, José A. G. da

; Carvalho, Ivan R.

; Colet, Christiane de F.

; Lucchese, Osório A.

; Basso, Natiane C. F.

; Alessi, Odenis

; Sgarbossa, Jaqueline

; Dias, Jéssica E. L.

; Peter, Cibele L.

Revista Brasileira de Engenharia Agrícola e Ambiental

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Set 2022, Volume 26 Nº 9 Páginas 670 - 679

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO O manejo do nitrogênio analisando indicadores agronômicos e ambientais garante produtividade satisfatória com maior qualidade ambiental. O objetivo neste estudo é propor um manejo mais sustentável de uso do nitrogênio em trigo, considerando o fornecimento em dose cheia e fracionada com estimativa da eficiência técnica, econômica e de expectativa de produtividade. Dois experimentos foram conduzidos, um para quantificar a produtividade de biomassa e outro, para avaliação do rendimento de grãos. O delineamento experimental foi o de blocos casualizados com quatro repetições em fatorial 5 × 3, para doses de N-fertilizante (0, 30, 60, 90 e 120 kg ha-1) e forma de fornecimento do nutriente [condição cheia (100%) no estádio fenológico V3 (terceira folha expandida); fracionada (70/30%) no estádio fenológico V3/V6 (terceira e sexta folha expandida) e; fracionada (70/30%) no estádio fenológico V3/R1 (início da antese)], respectivamente. O manejo mais sustentável do nitrogênio em trigo é obtido com a dose de expectativa de produtividade de 3 t ha-1 pela adubação manual, com o nutriente fornecido em dose cheia no estádio fenológico V3.

ABSTRACT The management of nitrogen by the assessment of agronomic and environmental indicators ensures satisfactory productivity with higher environmental quality. The objective of this study was to propose more sustainable management of nitrogen use in wheat, considering the supply of full and fractioned doses with an estimate of technical, economic efficiency, and expected productivity. Two experiments were conducted, one to quantify biomass productivity and the other to evaluate grain yield. The experimental design consisted of a randomized block with four repetitions in a 5 × 3 factorial, for N-fertilizer doses (0, 30, 60, 90, and 120 kg ha-1) and nutrient supply form [full condition (100%) in the phenological stage V3 (the third leaf expanded); fractionated (70/30%) in the phenological stage V3/V6 (third and sixth leaf expanded) and; fractionated (70/30%) in the phenological stage V3/R1 (beginning of anthesis)], respectively. The most sustainable management of nitrogen in wheat was obtained with the expected yield rate of 3 t ha-1 by manual fertilization, with nitrogen supplied in full dose at the V3 phenological stage.

https://doi.org/10.1590/1807-1929/agriambi.v26n9p670-679

Art and science: impact of semioccluded vocal tract exercises and choral singing on quality of life in subjects with congenital GH deficiency
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Andrade, Bruna M. R. de

; Valença, Eugenia H. O.

; Salvatori, Roberto

; Oliveira Neto, Luiz A.

; Souza, Anita H. O.

; Oliveira, Alaíde H. A.

; Oliveira, Mario C.P.

; Melo, Enaldo V.

; Carvalho, Susana de

; Sales, Neuza J

; Monteiro, Gisane C.

; Lima, José Marcel de

; Annunziato, Marcos Felipe Harder

; Mannis, Guilherme Daniel Breternitz

; Souza, Lucas E. de A.

; Goes, Yasmin D.

; Carvalho, Thayza S.

; Farias, Celiane de

; Santos, Michela P. dos

; Cardoso, Gabriela P. F.

; Sousa, Carla S. Pereira

; Santana, Julia Rodrigues

; Sales, Ester Almeida

; d’Avila, Jeferson Sampaio

; Aguiar-Oliveira, Manuel H.

Archives of Endocrinology and Metabolism

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Abr 2022, Volume 66 Nº 2 Páginas 198 - 205

Resumo: En | Texto: En | PDF: En

ABSTRACT Objectives: Currently, not much is known about the interactions between voice and growth hormone (GH). We have described large kindred with isolated GH deficiency (IGHD) due to a GHRH receptor mutation, resulting in severe short stature and high-pitched voice. These IGHD individuals have little interest in GH treatment, as they consider themselves “short long-lived people”, rather than patients. Interestingly, they report normal general quality of life, but they rate their Voice-Related Quality of Life (V-RQOL) as low. Here, we assessed the social and auditory-perceptual impacts of artistic-intervention voice therapy with semioccluded vocal tract exercises (SOVTE) and choral singing, on their voices. Material and methods: Seventeen GH-naïve adult IGHD individuals were enrolled in a single-arm interventional pre-post study with 13 weekly sessions of choir singing over 90 days. Outcome measures were V-RQOL scores, self-assessment of voice, and auditory-perceptual analysis (GRBAS scale, G: grade of the severity of dysphonia; R: roughness; B: breathiness; A: asthenia; and S: strain). Results: Marked improvements in total (p = 0.0001), physical (p = 0.0002), and socioemotional (p = 0.0001) V-RQOL scores and in self-assessment of voice (p = 0.004) were found. The general grades of vocal deviation (p = 0.0001), roughness (p = 0.0001), breathiness (p = 0.0001) and strain (p = 0.0001) exhibited accentuated reductions. Conclusions: Voice therapy with semioccluded vocal tract exercises and choral training improved social impact and perceptual voice assessments in IGHD subjects and markedly improved their voice-related quality of life. This is particularly important in a setting where GH replacement therapy is not widely accepted.

https://doi.org/10.20945/2359-3997000000449

Cordyceps cateniannulata and Cordyceps javanica: first report of pathogenicity to Glycaspis brimblecombei (Hemiptera: Aphalaridae)
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DOMINGUES, MAURÍCIO M.

; SANTOS, PAULA L.

; GÊA, BIANCA C.C.

; CARVALHO, VANESSA R.

; OLIVEIRA, FABRICIO N.

; SOLIMAN, EVERTON P.

; PEREIRA, FABRICIO F.

; ZANUNCIO, JOSÉ C.

; WILCKEN, CARLOS F.

Anais da Academia Brasileira de Ciências

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2022, Volume 94 elocation e20211566

Resumo: En | Texto: En | PDF: En

Abstract Cultivation of species of the genus Eucalyptus is important for the Brazilian economy, with 6.97 million hectares planted. Glycaspis brimblecombei Moore (Hemiptera: Aphalaridae), detected in Brazil in 2003, has dispersed and now damages Eucalyptus crops in all regions of this country. The location and identification of entomopathogenic fungi isolates may increase the options for integrated pest management. The objective of this research was to evaluate the pathogenicity of Cordyceps cateniannulata and Cordyceps javanica isolates to G. brimblecombei. Ten nymphs of G. brimblecombei, with or without lerps, were placed per Eucalyptus leaf cut with one of its edges on hydroretentive gel inside Petri dishes. The fungi isolates were suspended in a solution of Tween 80 (0.1%) at the concentration of 1.0 x 108 conidia mL-1 and sprayed on the G. brimblecombei nymphs. The mortality of this insect was evaluated daily for seven days, and the dead individuals were transferred to humid chambers. The conidia viability of the isolates was greater than 93%. The mortality of G. brimblecombei nymphs, seven days after the application of the fungi, was 100%. This is the first report of the pathogenicity of C. cateniannulata and C. javanica isolates, occurring naturally in the field, to G. brimblecombei.

https://doi.org/10.1590/0001-3765202220211566

Aquatic pollutants are associated with reproductive alterations and genotoxicity in estuarine fish (Sciades herzbergii - Bloch, 1794) from the Amazon Equatorial Coast
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Noleto, K. S.

; Mendes, D. C. S.

; Carvalho, I. F. S.

; Ribeiro, D. L. S.

; Santos, D. M. S.

; Ferreira, A. P. M.

; Marques, A. L. B.

; Carvalho Neta, R. N. F.

; Tchaicka, L.

; Torres Júnior, J. R. S.

Brazilian Journal of Biology

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2022, Volume 82 elocation e267996

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Os estuários estão constantemente sujeitos a impactos ambientais de atividades antropogênicas, como pesca, movimentação portuária, indústria ou esgoto doméstico, onde os peixes são um dos animais aquáticos mais atingidos, sendo capazes de refletir os impactos diretamente em seu organismo. Assim, o objetivo desse estudo foi realizar o biomonitoramento de um estuário da Costa Equatorial Amazônica por meio de análises de PAHs (Hidrocarboneto Policiclico Aromáticos) na água, além de metais traços, alterações histopatológicas e análises de micronúcleos eritrocitários em Sciades herzbergii.. O S. herzbergii foi utilizado como espécie modelo, devido seu comportamento estuarino residente. Amostras de gônadas e brânquias foram submetidas a avaliações histopatológicas. A quantificação de metais traços foi realizada em amostras de músculos esqueléticos dos animais coletados, onde foram encontrados teores de Chumbo (Pb), Cobre (Cu), Zinco (Zn), Cádmio (Cd), Magnésio (Mg), Ferro (Fe) e Alumínio (AL), e foram encontradas em concentrações acima dos limites recomendados, à exceção do Cádmio (Cd). A análise dos PAHs revelou a presença de Naftaleno e Acenafteno nas amostras de água. Análises histopatológicas e genotóxicas revelaram lesões em 100% dos espécimes estudados, resultados estes, potencialmente associados à presença de PAH e de metais traços, tanto na água quanto nos tecidos animais, comprovando um cenário geral de contaminação ambiental implicando diretamente em risco à saúde e sobrevivência da biota local. Esse estudo mostra a relevância do biomonitoramento contínuo dos ecossistemas estuarinos, afim de nortear autoridades no manejo de efluentes e assegurar o desenvolvimento evolutivo de espécies estuarinas, sobretudo dos peixes de importância na culinária regional, diretamente relacionados à segurança alimentar humana.

Abstract Estuaries are constantly subject to continuous environmental impacts of human activities, such as fisheries, port or industry, and domestic sewage, with fish being one of the most affected aquatic animals, reflecting the impacts directly on their bodies. Thus, the aim of this study was to carry out the biomonitoring of an estuary located on the Amazonian Equatorial Coast through analysis of PAHs (Polycyclic Aromatic Hydrocarbons) in the water, in addition to trace metals, histopathological alterations and analysis of erythrocyte micronuclei in Sciades herzbergii. S. herzbergii was used as a model species, due to its estuarine-resident behavior. Gonad and gill samples were subjected to histopathological evaluations. The quantification of trace metals was performed in samples of skeletal muscles of the animals collected, where concentrations of Lead (Pb), Copper (Cu), Zinc (Zn), Cadmium (Cd), Magnesium (Mg), Iron (Fe) and Aluminum (Al) were found. Except for Cadmium (Cd), all the concentrations were above the recommended limits. The PAHs analysis revealed the presence of Naphthalene and Acenaphthene in the water samples Histopathological and genotoxic analyses revealed of lesions in 100% of the study specimens. Thus, the histological and genotoxic alterations found in 100% of S. herzbergii specimens captured in São José Bay-MA are potentially associated with PAH concentrations present in the water. These results are potentially associated with the presence of PAH and trace metals, both in water and in animal tissues, inferring a general scenario of environmental contamination which directly implies a risk to the health and survival of the local biota. This study shows the relevance of continuous biomonitoring of estuarine ecosystems, in order to guide authorities regarding sewage management and ensure the evolutionary development of estuarine species, especially fishes of importance in the local cuisine, therefore related to human food security.

https://doi.org/10.1590/1519-6984.267996

Use and misuse of biomarkers and the role of D-dimer and C-reactive protein in the management of COVID-19: A post-hoc analysis of a prospective cohort study
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Gonçalves, Fabio Augusto Rodrigues

; Besen, Bruno Adler Maccagnan Pinheiro

; Lima, Clarice Antunes de

; Corá, Aline Pivetta

; Pereira, Antônio José Rodrigues

; Perazzio, Sandro Félix

; Gouvea, Christiane Pereira

; Fonseca, Luiz Augusto Marcondes

; Trindade, Evelinda Marramon

; Sumita, Nairo Massakazu

; Duarte, Alberto José da Silva

; Lichtenstein, Arnaldo

; Bonfa, Eloisa

; Utiyama, Edivaldo M.

; Segurado, Aluisio C.

; Perondi, Beatriz

; Miethke-Morais, Anna

; Montal, Amanda C.

; Harima, Leila

; Fusco, Solange R. G.

; Silva, Marjorie F.

; Rocha, Marcelo C.

; Marcilio, Izabel

; Rios, Izabel Cristina

; Kawano, Fabiane Yumi Ogihara

; Jesus, Maria Amélia de

; Kallas, Ésper George

; Carmo, Carolina

; Tanaka, Clarice

; Souza, Heraldo Possolo de

; Marchini, Julio F. M.

; Carvalho, Carlos

; Ferreira, Juliana C.

; Levin, Anna Sara Shafferman

; Oliveira, Maura Salaroli

; Guimarães, Thaís

; Lázari, Carolina dos Santos

; Sabino, Ester

; Magri, Marcello M. C.

; Barros-Filho, Tarcisio E. P.

; Francisco, Maria Cristina Peres Braido

; Costa, Silvia F.

Clinics

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2021, Volume 76 elocation e3547

Resumo: En | Texto: En | PDF: En

OBJECTIVE: Coronavirus disease 2019 (COVID-19) is associated with high mortality among hospitalized patients and incurs high costs. Severe acute respiratory syndrome coronavirus 2 infection can trigger both inflammatory and thrombotic processes, and these complications can lead to a poorer prognosis. This study aimed to evaluate the association and temporal trends of D-dimer and C-reactive protein (CRP) levels with the incidence of venous thromboembolism (VTE), hospital mortality, and costs among inpatients with COVID-19. METHODS: Data were extracted from electronic patient records and laboratory databases. Crude and adjusted associations for age, sex, number of comorbidities, Sequential Organ Failure Assessment score at admission, and D-dimer or CRP logistic regression models were used to evaluate associations. RESULTS: Between March and June 2020, COVID-19 was documented in 3,254 inpatients. The D-dimer level ≥4,000 ng/mL fibrinogen equivalent unit (FEU) mortality odds ratio (OR) was 4.48 (adjusted OR: 1.97). The CRP level ≥220 mg/dL OR for death was 7.73 (adjusted OR: 3.93). The D-dimer level ≥4,000 ng/mL FEU VTE OR was 3.96 (adjusted OR: 3.26). The CRP level ≥220 mg/dL OR for VTE was 2.71 (adjusted OR: 1.92). All these analyses were statistically significant (p<0.001). Stratified hospital costs demonstrated a dose-response pattern. Adjusted D-dimer and CRP levels were associated with higher mortality and doubled hospital costs. In the first week, elevated D-dimer levels predicted VTE occurrence and systemic inflammatory harm, while CRP was a hospital mortality predictor. CONCLUSION: D-dimer and CRP levels were associated with higher hospital mortality and a higher incidence of VTE. D-dimer was more strongly associated with VTE, although its discriminative ability was poor, while CRP was a stronger predictor of hospital mortality. Their use outside the usual indications should not be modified and should be discouraged.

https://doi.org/10.6061/clinics/2021/e3547

Steinernema diaprepesi Nguyen & Duncan (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Brazil
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HORTA, ANDRÉ B.

; DAMASCENA, ALIXELHE P.

; CARVALHO, VANESSA R. DE

; RIBEIRO, MURILO F.

; CASTRO, BÁRBARA M.C.

; WILCKEN, CARLOS F.

; ZANUNCIO, JOSÉ C.

; WILCKEN, SILVIA R.S.

Anais da Academia Brasileira de Ciências

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2021, Volume 93 Nº 4 elocation e20190943

Resumo: En | Texto: En | PDF: En

Abstract Entomopathogenic nematodes (EPNs) can control pests due to their mutual association with bacteria. The use of these biological control agents is increasing worldwide due to advances in research about its control efficiency, range of action and mass production. The identification of EPNs adapted to specific environmental and climatic conditions is important for sustainable pest suppression in integrated management (IPM) programs. The objective is to report, for the first time, the occurrence of the Steinernema diaprepesi in Brazil. Steel mesh traps with Galleria mellonella Linnaeus (Lepidoptera: Pyralidae) larvae were buried in red latosol cultivated with Eucalyptus. Infective juveniles (IJs) were isolated from dead larvae and multiplied in healthy ones of this host to confirm its pathogenicity and to start a laboratory population from the strain found in the field. The DNA of the IJs was extracted and amplified using PCR technique with the universal primers D2A and D3B. The detection of S. diaprepesi is the first report of this nematode in Brazil, increasing the knowledge about its distribution in the world and the diversity of EPNs that must be considered as agents of biological pest control in the country.

https://doi.org/10.1590/0001-3765202120190943 79 downloads

Persistent symptoms and decreased health-related quality of life after symptomatic pediatric COVID-19: A prospective study in a Latin American tertiary hospital
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Fink, Thais T.

; Marques, Heloisa H.S.

; Gualano, Bruno

; Lindoso, Livia

; Bain, Vera

; Astley, Camilla

; Martins, Fernanda

; Matheus, Denise

; Matsuo, Olivia M.

; Suguita, Priscila

; Trindade, Vitor

; Paula, Camila S.Y.

; Farhat, Sylvia C.L.

; Palmeira, Patricia

; Leal, Gabriela N.

; Suzuki, Lisa

; Odone Filho, Vicente

; Carneiro-Sampaio, Magda

; Duarte, Alberto José S.

; Antonangelo, Leila

; Batisttella, Linamara R.

; Polanczyk, Guilherme V.

; Pereira, Rosa Maria R.

; Carvalho, Carlos Roberto R.

; Buchpiguel, Carlos A.

; Latronico, Ana Claudia

; Seelaender, Marilia

; Silva, Clovis Artur

; Pereira, Maria Fernanda B.

; Sallum, Adriana M. E.

; Brentani, Alexandra V. M.

; Neto, Álvaro José S.

; Ihara, Amanda

; Santos, Andrea R.

; Canton, Ana Pinheiro M.

; Watanabe, Andreia

; Santos, Angélica C. dos

; Pastorino, Antonio C.

; Franco, Bernadette D. G. M.

; Caruzo, Bruna

; Ceneviva, Carina

; Martins, Carolina C. M. F.

; Prado, Danilo

; Abellan, Deipara M.

; Benatti, Fabiana B.

; Smaria, Fabiana

; Gonçalves, Fernanda T.

; Penteado, Fernando D.

; Castro, Gabriela S. F. de

; Gonçalves, Guilherme S.

; Roschel, Hamilton

; Disi, Ilana R.

; Marques, Isabela G.

; Castro, Inar A.

; Buscatti, Izabel M.

; Faiad, Jaline Z.

; Fiamoncini, Jarlei

; Rodrigues, Joaquim C.

; Carneiro, Jorge D. A.

; Paz, Jose A.

; Ferreira, Juliana C.

; Ferreira, Juliana C. O.

; Silva, Katia R.

; Bastos, Karina L. M.

; Kozu, Katia

; Cristofani, Lilian M.

; Souza, Lucas V. B.

; Campos, Lucia M. A.

; Silva Filho, Luiz Vicente R. F.

; Sapienza, Marcelo T.

; Lima, Marcos S.

; Garanito, Marlene P.

; Santos, Márcia F. A.

; Dorna, Mayra B.

; Aikawa, Nadia E.

; Litvinov, Nadia

; Sakita, Neusa K.

; Gaiolla, Paula V. V.

; Pasqualucci, Paula

; Toma, Ricardo K.

; Correa-Silva, Simone

; Sieczkowska, Sofia M.

; Imamura, Marta

; Forsait, Silvana

; Santos, Vera A.

; Zheng, Yingying

Clinics

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2021, Volume 76 elocation e3511

Resumo: En | Texto: En | PDF: En

OBJECTIVES: To prospectively evaluate demographic, anthropometric and health-related quality of life (HRQoL) in pediatric patients with laboratory-confirmed coronavirus disease 2019 (COVID-19) METHODS: This was a longitudinal observational study of surviving pediatric post-COVID-19 patients (n=53) and pediatric subjects without laboratory-confirmed COVID-19 included as controls (n=52) was performed. RESULTS: The median duration between COVID-19 diagnosis (n=53) and follow-up was 4.4 months (0.8-10.7). Twenty-three of 53 (43%) patients reported at least one persistent symptom at the longitudinal follow-up visit and 12/53 (23%) had long COVID-19, with at least one symptom lasting for >12 weeks. The most frequently reported symptoms at the longitudinal follow-up visit were headache (19%), severe recurrent headache (9%), tiredness (9%), dyspnea (8%), and concentration difficulty (4%). At the longitudinal follow-up visit, the frequencies of anemia (11% versus 0%, p=0.030), lymphopenia (42% versus 18%, p=0.020), C-reactive protein level of >30 mg/L (35% versus 0%, p=0.0001), and D-dimer level of >1000 ng/mL (43% versus 6%, p=0.0004) significantly reduced compared with baseline values. Chest X-ray abnormalities (11% versus 2%, p=0.178) and cardiac alterations on echocardiogram (33% versus 22%, p=0.462) were similar at both visits. Comparison of characteristic data between patients with COVID-19 at the longitudinal follow-up visit and controls showed similar age (p=0.962), proportion of male sex (p=0.907), ethnicity (p=0.566), family minimum monthly wage (p=0.664), body mass index (p=0.601), and pediatric pre-existing chronic conditions (p=1.000). The Pediatric Quality of Live Inventory 4.0 scores, median physical score (69 [0-100] versus 81 [34-100], p=0.012), and school score (60 [15-100] versus 70 [15-95], p=0.028) were significantly lower in pediatric patients with COVID-19 at the longitudinal follow-up visit than in controls. CONCLUSIONS: Pediatric patients with COVID-19 showed a longitudinal impact on HRQoL parameters, particularly in physical/school domains, reinforcing the need for a prospective multidisciplinary approach for these patients. These data highlight the importance of closer monitoring of children and adolescents by the clinical team after COVID-19.

https://doi.org/10.6061/clinics/2021/e3511

10.

Growing vegetables in succession in different soils and doses of phosphorus in an organomineral fertilizer
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Vieira, Dinamar Márcia da S.

; Camargo, Reginaldo de

; Torres, Jose Luiz R.

; Silva, Adriane de A.

; Lana, Regina M. Q.

; Carvalho, Fabio J.

Revista Brasileira de Engenharia Agrícola e Ambiental

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Dez 2020, Volume 24 Nº 12 Páginas 806 - 813

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO O objetivo deste estudo foi avaliar o teor residual da aplicação de doses fosforo (P) via fertilizante organomineral (FO) em dois solos cultivados com repolho e alface em sucessão. Em blocos casualizados, utilizou-se cinco doses de FO como fonte de P. No Oxisol aplicaram-se: 0; 50; 100; 200 e 300% da dose de P recomendada para o repolho com utilização de FO, mais um tratamento com 100% da dose via fertilizante mineral. No Entisol aplicaram-se: 0; 50; 100; 200 e 300% da dose de P com FO para a cultura do repolho, além de um tratamento adicional (100% mineral), com quatro repetições. Avaliaram-se a produção de massa fresca (MF) e seca (MS), estado nutricional do repolho e da alface e o efeito residual de nutrientes no solo. O teor residual do P no solo aumentou (p ≤ 0,05) quanto maior foi à dose de FO utilizada no Oxisol nos dois ciclos avaliados, enquanto que no Entisol isto só ocorreu no primeiro ciclo. Usando 50% da dose de FO no repolho em ambos os solos proporciona produção de MF e MS no mínimo 11% superior, comparado à adubação mineral.

ABSTRACT The objective of this study was to evaluate the residual concentration from the application of levels of phosphorus contained in an organomineral fertilizer (OMF) in two soils cultivated with cabbage and lettuce in succession. In randomized blocks, five doses of OMF were used as a source of P in Oxisol (0, 50, 100, 200, and 300% of the P dose was recommended for cabbage crop using OMF), plus an additional treatment (100% mineral). The doses applied to the Entisol were as follows: 0, 50, 100, 200, and 300% of the recommended P dose with OMF for cabbage crop, plus an additional treatment (100% mineral), with four repetitions. The production of fresh matter (FM) and dry matter (DM), the nutritional status of the cabbage and lettuce, and the residual effect of nutrients in the soil were also evaluated. The P concentration in the soil increased (p ≤ 0.05) with higher doses of OMF in the Oxisol in the two cycles evaluated. However, this occurred only in the first cycle in the Entisol. Using the 50% dose of OMF in the cabbage in both soils provided FM and DM production values that were at least 11% higher than those attained with mineral fertilizer.

https://doi.org/10.1590/1807-1929/agriambi.v24n12p806-813 627 downloads

11.

Phenotypic adaptability and stability of herbaceous cotton genotypes in the Semiarid region of the Northeast of Brazil
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Silva, Ruan dos S.

; Farias, Francisco J. C.

; Teodoro, Paulo E.

; Cavalcanti, José J. V.

; Carvalho, Luiz P. de

; Queiroz, Damião R.

Revista Brasileira de Engenharia Agrícola e Ambiental

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Dez 2020, Volume 24 Nº 12 Páginas 800 - 805

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO A cultura do algodoeiro (Gossypium hirsutum L.) na região do Nordeste brasileiro é submetida a alta variabilidade edafoclimática, o que contribui para a ocorrência de forte interação genótipo versus ambiente (G x A). Nessa situação, faz-se necessário o uso de metodologias de adaptabilidade e estabilidade para o estudo da interação G x A antes da seleção e recomendação de cultivares. Dentre estas, a análise genótipo + G x E (GGE) biplot se destaca como uma das mais recomendadas e usadas atualmente. O objetivo deste estudo foi avaliar a interação genótipo versus ambiente (G × A) de linhagens finais de algodoeiro por meio da metodologia GGE biplot, visando identificar genótipos adaptados e estáveis para as condições do semiárido nordestino. Foram conduzidos quatro ensaios de valor de cultivo e uso nas cidades de Apodi, RN, e Barbalha, CE, Brasil, nos anos de 2016 e 2017. O delineamento experimental foi de blocos casualizados, com 17 tratamentos e quatro repetições. Os tratamentos foram constituídos de 17 genótipos de algodoeiro. As variáveis avaliadas foram: produtividade de algodão em caroço e produtividade de fibra. A análise identificou as linhagens CNPA BA 2011-4436, CNPA BA 2011-1197 e CNPA BA 2010-1174 como estáveis e com ampla adaptabilidade para a região avaliada.

ABSTRACT Cotton (Gossypium hirsutum L.) crop areas in the Northeast region of Brazil present high edaphoclimatic variability, which contributes to a strong genotype versus environment interaction (G × E). This situation requires the use of adaptability and stability methodologies to study G × E interaction before the selection and recommendation of cultivars. Among these methodologies, the genotype + G × E (GGE) biplot analysis has been currently used and highly recommended. Thus, the objective of this study was to evaluate the G × E of cotton genotypes through GGE biplot analysis, focusing on the identification of adapted and stable genotypes for the Semiarid region of the Northeast of Brazil. Four crop value and use tests were conducted in the municipalities of Apodi, state of Rio Grande do Norte, and Barbalha, state of Ceará, Brazil, in 2016 and 2017. A randomized block experimental design, with 17 treatments and four repetitions, was used. The treatments consisted of 17 cotton genotypes. The variables evaluated were: cotton seed and fiber yields. According to the analysis, the genotypes CNPA BA 2011-4436, CNPA BA 2011-1197, and CNPA BA 2010-1174 were stable and presented high adaptability to the evaluated region.

https://doi.org/10.1590/1807-1929/agriambi.v24n12p800-805 419 downloads

12.

Tenofovir Disoproxil Fumarate: New Chemical Developments and Encouraging in vitro Biological Results for SARS-CoV-2
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Clososki, Giuliano C.

; Soldi, Rafael A.

; Silva, Rodrigo M. da

; Guaratini, Thais

; Lopes, José N. C.

; Pereira, Pâmela R. R.

; Lopes, João L. C.

; Santos, Thiago dos

; Martins, Ronaldo B.

; Costa, Cristina S.

; Carvalho, Andréia N. de

; da Silva, Luis L. P.

; Arruda, Eurico

; Lopes, Norberto P.

Journal of the Brazilian Chemical Society

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Ago 2020, Volume 31 Nº 8 Páginas 1552 - 1556

Resumo: En | Texto: En | PDF: En

The recent emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led society to live with a serious public health problem. In this sense, repositioning of antiretrovirals has captured the attention of the scientific community. Tenofovir disoproxil fumarate (TDF) is an antiretroviral compound that is used to treat acquired immune deficiency syndrome (AIDS) and hepatitis B. In this short report, we present a scale-up investigation of TDF by in situ infrared spectroscopy monitoring and a forced degradation study to describe a new degradation product. Finally, we have evaluated TDF in vitro for SARS-CoV-2 for the first time foreseeing the using of this medicine in pre-clinical and clinical investigations for the COVID-19 (coronavirus disease 2019) treatment.

https://doi.org/10.21577/0103-5053.20200106 1823 downloads

13.

Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research
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COELHO, CATARINA A.

; BORDELO, JOÃO P.

; CAMASSA, JOSÉ A.

; BARROS, VERA A.

; BABO, PEDRO S.

; GOMES, MANUELA E.

; REIS, RUI L.

; AZEVEDO, JORGE T. DE

; REQUICHA, JOÃO F.

; FAÍSCA, PEDRO

; CARVALHO, PEDRO P.

; VIEGAS, CARLOS A.

; DIAS, ISABEL R.

Anais da Academia Brasileira de Ciências

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2020, Volume 92 Nº 4 elocation e20200435

Resumo: En | Texto: En | PDF: En

Abstract Osteoporosis is a metabolic disorder characterized by a loss of bone mass and structure and increasing the risk of fragility fractures, mostly among postmenopausal women. Sheep is a recognized large animal model for osteoporosis research. An experimental group of ewes (3-4 years old) was subjected to ovariectomy (OVX) and weekly glucocorticoid (GC) application for 24 weeks and compared with a sham control group. Blood and bone marrow parameters were analyzed before and 24 weeks after OVX and GC administration. Osteopenia was confirmed through micro-computed tomography and histomorphometric analysis of L4 vertebra in the study end. A statistically significant increase was observed in mean corpuscular volume, mean cell hemoglobin and monocytes and a decrease in red blood count and eosinophils (p<0.05). Alkaline phosphatase (ALP), gamma-glutamyl transpeptidase, magnesium and α1-globulin increased, and creatinine, albumin, sodium and estradiol decreased (p<0.05). A slight decrease of bone formation markers (bone ALP and osteocalcin) and an increase of bone resorption markers (C-terminal telopeptides of collagen type 1 and tartrate-resistant acid phosphatase) were observed, but without statistical significance. This study aims to contribute to better knowledge of sheep as a model for osteoporosis research and the consequences that a performed induction protocol may impose on organic metabolism.

https://doi.org/10.1590/0001-3765202020200435 677 downloads

14.

Freshwater fish richness baseline from the São Francisco Interbasin Water Transfer Project in the Brazilian Semiarid
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Silva, Márcio J.

; Ramos, Telton P. A.

; Carvalho, Fernando R.

; Brito, Marcelo F. G.

; Ramos, Robson T. C.

; Rosa, Ricardo S.

; Sánchez-Botero, Jorge I.

; Novaes, José L. C.

; Costa, Rodrigo S.

; Lima, Sergio M. Q.

Neotropical Ichthyology

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2020, Volume 18 Nº 4 elocation e200063

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Dentre as ecorregiões dulcícolas neotropicais, a Nordeste Médio-Oriental (MNCE) é uma lacuna de conhecimento sobre peixes. Suas drenagens temporárias são receptoras do projeto de transposição do São Francisco (SFR-IWT) no semiárido brasileiro. Fornecemos uma linha de base abrangente da riqueza de peixes das cinco bacias do SFR-IWT. Riqueza de espécies, espécies compartilhadas, endêmicas, ameaçadas e não-nativas foram obtidas através de amostragem, coleções ictiológicas, literatura e repositórios online (306 localidades). No total, foram registradas 121 espécies, 111 nativas e 16 (14,41%) listadas para todas as bacias. A maior riqueza de espécies nativas (78, 70,27%) foi registrada no trecho sub-médio do São Francisco (SFRE), incluindo 23 endêmicas e 61 (54,95%) nas bacias da MNCE (13 endêmicas), e 28 (25,23 %) compartilhadas entre ambas ecorregiões. Na MNCE, foram registradas 50 espécies na Jaguaribe (JAG), 39 na Piranhas-Açu, 36 na Paraíba do Norte e 32 na Apodi-Mossoró (APO). O número de espécies compartilhadas entre SFRE e cada bacia receptora variou de 24 (23,08%, JAG) a 20 (22,22%, APO). JAG contém 81,97% das espécies das bacias receptoras. Sua maior riqueza e endemismo merecem atenção especial em relação às mudanças hidrológicas em curso. Este estudo ajudará a detectar possíveis modificações na ictiofauna das principais drenagens da MNCE.

Abstract Among Neotropical freshwater ecoregions, the Mid-Northeastern Caatinga (MNCE) is a fish knowledge gap. Its temporary drainages are receptors of the São Francisco interbasin water transfer project (SFR-IWT) in the Brazilian semiarid. We provide a comprehensive baseline of fish richness of the five SFR-IWT basins. Species richness, shared, endemic, threatened and non-native species were obtained using sampling, ichthyologic collections, literature and online repositories (306 localities). In total 121, species were recorded, 111 of them native, and 16 (14.41%) listed for all basins. Higher richness of native species (78, 70.27%) was recorded in the São Francisco lower-middle stretch (SFRE), including 23 endemic, 61 (54.95%) in MNCE basins (13 endemic), and 28 (25.23%) shared between both ecoregions. In the MNCE, 50 species were recorded in Jaguaribe (JAG), 39 in Piranhas-Açu, 36 in Paraíba do Norte, and 32 in Apodi-Mossoró (APO). The number of species shared between the SFRE and each receptor basin varied from 24 (23.08%, JAG) to 20 (22.22%, APO). JAG contains 81.97% of the receptor basins’ species. Its higher richness and endemism deserve special attention regarding the ongoing hydrological changes. This study will help detect possible modifications in the ichthyofauna of the main MNCE drainages.

https://doi.org/10.1590/1982-0224-2020-0063 1387 downloads

15.

SPRAY RETENTION ON COFFEE LEAVES ASSOCIATED WITH TYPE AND CONCENTRATION OF ADJUVANTS
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Cação, José E. da C. R.

; Moreira, Bruno R. de A.

; Raetano, Carlos G.

; Carvalho, Fernando K.

; Prado, Evandro P.

Engenharia Agrícola

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Out 2019, Volume 39 Nº 5 Páginas 623 - 629

Resumo: En | Texto: En | PDF: En

ABSTRACT Agricultural spray adjuvants (ASA) are widely used in pesticide applications to enhance the performance of pesticides. The aim of this research was to investigate the effects of ASA on static surface tension (SST) and foliar spray retention on coffee leaves. The SST of ASA at different concentrations was determined by the drop weight method. Spray retention on adaxial and abaxial coffee leaf surfaces was performed using a micro-sprayer at solution concentrations of 0, 0.01, 0.1, 0.5, and 1.0% v v−1. The ASA assessed were: polyether-polymethylsiloxane-copolymer (PPC); Nonylphenol ethoxylate; nonyl polyethylene glycol ether; mineral oil; nonylphenoxy polyethoxy ethanol; carboxyl copolymer of styrene and butadiene; primary aliphatic oxyalkylated alcohol plus carboxyl copolymer of styrene and butadiene; and soyal phospholipids and propionic acid. All ASA reduced the SST of the aqueous solutions. PPC provided the best performance in decreasing SST, reaching values below 20 mN m−1 at a concentration of 0.05% v v−1. Spray retention on leaves was influenced by adjuvant type as well as concentration. A very strong positive correlation between SST and spray retention on coffee leaves was observed. Decreasing the SST of the solution provided a reduction of spray retention when spraying was performed until run-off point.

https://doi.org/10.1590/1809-4430-eng.agric.v39n5p623-629/2019 869 downloads

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