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Methodological development of a quality index for agricultural operations, for corn cultivation using multi-criterion analysis

Abstracts

One of the major problems in the Brazilian agriculture is related to the production loss in the field, which, due to many factors, is not being considered. This article has the objective to develop a methodology to identify a quality index which integrates some crop quality variables which are able to indicate how the crop is developing in terms of loss in the field. The results have showed that the strategy of multi-criterion analysis and Fuzzy logic proved to be important tools for the assessment and preparation of a quality index for corn production. The index proposed performed well in representing the quality of agricultural operations when compared with reality.

annual crop; optimization; fuzzy; crop quality


Um dos maiores problemas na agricultura brasileira refere-se à perda da produção no campo que, devido a vários fatores, não é considerada. Este artigo tem o objetivo de desenvolver uma metodologia para identificar um índice de qualidade que integre algumas variáveis qualitativas da cultura que são capazes de mostrar como está o desenvolvimento em termos de perda no campo. Os resultados mostraram que a análise de multicritério e lógica fuzzy são ferramentas importantes na verificação e confecção de um índice de qualidade de perdas, para a cultura do milho. O índice calculado representou bem a qualidade das operações agrícolas, quando comparado com a realidade.

cultura anual; otimização; fuzzy; qualidade de cultura


AGRARIAN SCIENCES

Methodological development of a quality index for agricultural operations, for corn cultivation using multi-criterion analysis

Rubens A.C. LamparelliI; Hugo S. DiasII

ICentro de Pesquisas Meteorológicas e Climáticas Aplicadas à Agricultura, UNICAMP Cidade Universitária Zeferino Vaz s/n, Barão Geraldo, 13083-970 Campinas, SP, Brasil

IILaboratório de Geoprocessamento, Faculdade de Engenharia Agrícola Caixa Postal 6011, 13083-875 Campinas, SP, Brasil

Correspondence to Correspondence to: Rubens Augusto Camargo Lamparelli E-mail: rubens@cpa.unicamp.br

ABSTRACT

One of the major problems in the Brazilian agriculture is related to the production loss in the field, which, due to many factors, is not being considered. This article has the objective to develop a methodology to identify a quality index which integrates some crop quality variables which are able to indicate how the crop is developing in terms of loss in the field. The results have showed that the strategy of multi-criterion analysis and Fuzzy logic proved to be important tools for the assessment and preparation of a quality index for corn production. The index proposed performed well in representing the quality of agricultural operations when compared with reality.

Key words: annual crop, optimization, fuzzy, crop quality.

RESUMO

Um dos maiores problemas na agricultura brasileira refere-se à perda da produção no campo que, devido a vários fatores, não é considerada. Este artigo tem o objetivo de desenvolver uma metodologia para identificar um índice de qualidade que integre algumas variáveis qualitativas da cultura que são capazes de mostrar como está o desenvolvimento em termos de perda no campo. Os resultados mostraram que a análise de multicritério e lógica fuzzy são ferramentas importantes na verificação e confecção de um índice de qualidade de perdas, para a cultura do milho. O índice calculado representou bem a qualidade das operações agrícolas, quando comparado com a realidade.

Palavras-chave: cultura anual, otimização, fuzzy, qualidade de cultura.

INTRODUCTION

The winter corn began to be planted in the State of Paraná in the mid-1980. With the end of the government's policy of subsidizing the production of wheat, a vertiginous growth of the area of the winter corn cultivation starts in the Center-South of Brazil, with the Middle-Paranapanema in the State of São Paulo standing out due to the improvement of technology applied to this crop (CONAB 2007).

In spite of recent gains, the difference between the productivity of experimental trials and that of commercial crops is enormous, and is linked to the lacking use of technology by farmers, the key factor to explain such different ways in the quality with which the operations are carried out. The distribution of the plants along the line of planting, the crop mulch being kept on the soil, the density of the plants, the infestation of weeds and the loss of crops are just some of the factors that have determined the difference between sowing in experiments and that of farmers. (IEA 2002). De Maria et al. (1999) have shown that 60% of the crops of winter corn, in the middle-Paranapanema, had some problem of soil compacting probably due to preparation without ideal conditions of dampness, while 8% of the used land was unsuitable for the crop given to its low contents of phosphorus and low saturation by bases.

A brief review of international literature relates agriculture to the quality of the water (Honisch et al. 2002, Benson et al. 2006), the soil (Andrews and Carroll 2001, Gruhn et al. 2000) and the environment (Biggs et al. 2007), but not exactly to those factors linked to farming operations.

Even though techniques for the quality control of processes have been developed by and for the industrial sector, their use displays great potential in the quality control of agricultural processes (Bonilha 1994, Milan and Fernandes 2002). Measurements of central trends in the control of a production process, such as means, are fundamental in quality control, but not enough for their proper characterization. Control of variability is vital to characterize the process and also to indicate the causes of problems within the process (Bonilha 1994, Peche Filho 1999, 1994). Some quality control programs make use of the coefficient of variation, CV, as a measure of dispersion (Pimentel-Gomes 1978, Garcia 1989).

Scapim et al. (1995), analyzing the results of 66 tests in the area of plant enhancement and genetics, proposed a classification of the coefficients of variation for some characteristics of corn growing, such as height of the plant, weight of 100 grains, height, weight and number of the ears, weight of the grains and prolificity, with a view to appraising the accuracy of the experiments. Based on the criterion put forward by Garcia (1989), the coefficient of variation (CV) was classified by those authors by virtue of the mean (m) and standard deviation (sd) of the CV of the 66 studied tests. The authors concluded that the limits of classes, proposed by Garcia (1989), are similar to those proposed by Pimentel-Gomes (1978) whose studied characteristics are much influenced by the ambient (weight and number of the ears, weight of grains and prolificity). However, for variations such as height of the plant and weight of 100 grains, Garcia's method (1989) is better suited as it proposes smaller class intervals.

Molin (2002), appraising relative productivity for corn, soybean and wheat during four harvesting seasons, used as minimum limit a coefficient of variation of 30% to represent the variability between the crops (variability of productivity over time). Above this value the productivity was considered non constant.

The lack of uniformity of density is so great in the cultivation of corn that many studies have sought methods for the correction of productivity due to densities in experimental portions. Even in experimental plots for variety competition, for research purpose, the final densities of eight experiments analyzed by Schmidt et al. (2001) obtained coefficients of variation ranging from 3.92% to 12.82%.

Milan and Fernandes (2002) used histograms, control charts and a T test for the statistical control of the preparation (chisel and grading tillage) of Ultissol soil in the region of Piracicaba, São Paulo State in two treatments, with and without quality control during performance. For one part of the preparation, the scarification, quality control provided acceptable levels on the control chart and reduced the variability of the data. On the other hand, grading, quality control likewise reduced the variability of the data but did not allow the achievement of the specified standards as the limits were set on theoretical bases, without a preliminary practical test (field conditions).

Laying down standards of quality control (limits of specification, means and variability) in the assessment of certain quality variables in the growing of winter corn is fundamental for starting up a quality program. The choice of the variables that may be used in the quality control process is not easy. The analysis must take into account the importance and hierarchy among them. Multi-criterion decision analysis is a manner widely used in such cases.

It is the field of study that considers a decision in the presence of two or more conflicting objectives, providing the decision-maker with tools for the choice of the best alternative (Tecle and Duckstein 1994, Pieter- sen 2006).

Many works have used this technique for this purpose (Pietersen 2006, Gilliams et al. 2005) with particular emphasis on the work of Baja et al. 2007 who used the Multi-criterion method and the GIS (Geographical Information System) to generate two indices of Earth sustainability. The work shows the importance of the Multi-criterion method in the derivation of quantitative indices for natural phenomena.

The aim of this work was to suggest an operational quality index for the cultivation of winter corn using the Multi-criterion method, taking into consideration hierarchical analysis and Fuzzy Logic by using data from certain items of verification.

MATERIALS AND METHODS

The studied region is bounded by the coordinates 50°48'17.6" W / 22°58'5.15" S and 50°24'58.4" W/ 22°36'20.9" S in the South of São Paulo State, Brazil.

From the standpoint of mapping-out the operational quality of the crops, the areas were submitted to spatial analysis on the date of sowing, crop mulch (%) (mean of the planted strip and coefficient of variation of the strip); interline spacing (cm) (mean of the planted strip and coefficient of variation of the strip); density (number of plants per meter) (mean of the strip); number of acceptable spaces greater than 0.10 m and smaller than 0.30 m (plants properly positioned); number of plants per meter (mean of the planted strip); length of gaps greater than 0.30 m (mean of the strip) (m m-1), infestation of weeds in the development of the crop and after harvest (proportion of the area covered with weeds) (mean of the strip); loss of crop (total) (mean of the strip) (kg ha-1).

Considering the large number of semivariograms assessed for each variable, a standard of analysis was established to simplify exposition of the results. Initially semivariograms were sought to prove the existence of spatial correlation (Souza Dias 2006). Secondly, through an intensive analysis of the crossed validations, we sought the model with the best cross validation result (Souza Dias 2006). Spatialization of the variables during the development of the crops was done on the basis of 108 planted strips which were sampled, while the variables analyzed after the harvest were performed on 156 sampled strips.

The classification of the quality of the crops was performed through the IDRISI 32 decision-support modules using maps of the quality items of the crops produced by kriging method of the values of the means encountered in the sampled strips.

A careful analysis of the available items of quality verification makes it clear that these items are not sufficiently wide-ranging for the classification and mapping-out of the overall quality of the crops of winter corn. The lack of important control items, such as: productivity, quality of the grains and an analysis of sus-tainability of the production system prevents a broader analysis of quality. As a result we established that the analysis would be of operational quality in the cultivation, being it the maximum that the chosen items of verification would allow.

VERIFICATION ITEMS OF OPERATIONAL QUALITY ESTIMATE OF THE DATE OF SOWING

The estimate of the date of sowing was made by subtracting from the date of post-harvest sampling the number of days necessary to accumulate 1661 daily thermal units (DTU's), and subtracting another two days (considering the average time between the harvest and the sampling) (IAC 2001, Fornasieri Filho 1992).

CROP MULCH

The proportion of the soil covered by vegetable wastes, known as crop mulch, was established during the development of the corn growth and immediately after its harvest, making a visual estimation of the area covered with harvested waste in the first five spaces among the lines, counting from the top downwards (Fig. 2). Remarkably, at the fifth sampling point of the strip, after the visual assessment, an assessment was made of crop mulch by the string method (Laflen et al. 1981). It was done to verify the accuracy ofthe visual assessment. The crop mulch was expressed as a percentage (%).


SPACING AMONG THE LINES

Spacing among the lines was evaluated by measuring the distances of five spaces among the lines of plants in each sample with a tape measure. This variable was expressed in centimeters (cm) (Fig. 2).

DENSITY AND POPULATION OF PLANTS

The density, or number of plants per linear meter (pl/m), was determined by counting the number of plants in five linear meters in the first five lines of planting of the sample, from the top downwards (Fig. 2).

SPACING AMONG PLANTS

On the 4th line, with a view to assessing the distribution of plants along 5 meters of line, we counted the number of plants in each space of 0.10 m. The assessment of spacing among plants was indirect. The counting and calculation of the number and size of the spaces among plants and the assessment of the uniformity of distribution of plants were performed using a computer program developed in Visual Basic. The criteria employed were adapted from those recommended by Kurachi et al. (1989). An assessment was made of the acceptable spaces, per meter of line (no. m-1), greater than 0.10 m and smaller than 0.30m.

Based on the number of acceptable spaces and the average length of the spaces among plants, we estimated the length of acceptable spaces greater than 0.10 m and smaller than 0.30 m. These variables were expressed in meters of acceptable spaces per meter of line (m m-1).

The number of multiple spaces was also assessed (agglomerated plants), which means the number of spaces smaller than 0.20 m with two or more plants. This variable was expressed in numbers of multiple spaces per meter of line (no. m-1).

Two sizes of gaps were counted: the number of spaces without plants greater than 0.30 m and the number of spaces without plants greater than 0.50 m. These variables were expressed in numbers of gaps per meter of line (no. m-1).

Based on the number of gaps and the mean length of the gaps (Dambrós et al. 1998), we estimated the length of gaps greater than 0.30 m, expressed in meters of gap per meter of line (m m-1).

INFESTATION OF WEEDS

The infestation of weeds (proportion, in % of area, of the surface of soil occupied by weeds) was assessed visually by an estimate of the percentage of area occupied by weeds within a square of 25 cm2, as per the scale proposed by Braun-Blanquet (1932), quoted by Blanco (1977).

LOSS OF CROP

The methodology applied was adapted from the system proposed by Finch et al. (1980), Mantovani (1989) and Fornasieri Filho (1992). Loss of crop was assessed through the manual collection of loose grains in a rectangle of five meters in length by one meter in width (5 m2) in such a way that the longer side ran perpendicular to the planted lines. The structures (ear, cobs) with grains were collected manually over a whole square (sample) of 5 meters along the side (25 m2). All grains found on the surface of the soil were collected, identified, classified, husked, cleaned, dried and weighed. They were classified into: loose grains on the ground (grains that normally pass through the track of the harvester, but which are thrown away); grains fixed to pieces of cob (structures that pass through the harvester's track system); ears, normally whole (that have not passed through the harvester's track system); and, finally, technical loss of crop (total sum of the previous three). This variable was expressed in kilograms of material lost per hectare (km ha-1).

CRITERIA OF HIERARCHICAL TREE

For the development of the multi-criterion analysis, it is fundamental to make a careful definition of the objectives, as this guides the process of decision or choice (Forman and Selly 2001). We establish the weightings for the criteria based on these objectives.

A clear definition of the criteria allows one to set the degree of relative importance among them which will be used in the construction of the dual-comparison matrix. This matrix will calculate the relative weightings of the criteria that will be used in the multi-criterion analysis.

In the first step to multi-criterion analysis, we created a criteria of hierarchical tree (Fig. 3) seeking an organization of these criteria into levels, which would subsequently allow a rationalization in the distribution of weightings. The criteria were subdivided into two levels. At the First level we have the criterion of assessment of the factor that directly influenced the operational quality of the cultivation (crop mulch). At the Second level there were the criteria that influenced directly the First level criteria, such as the mean of crop mulch on the strip and the variability of crop mulch on it.


The hierarchy of criteria was established during the assembly of the tree of criteria, grouping together all the sub-criteria that concern to one same criterion at a higher level. In this way, an attempt was made to avoid overlapping in the assessment. Once the criteria are on one selfsame hierarchical level, they can be compared and their weighting can be set.

The Second level criteria break down each First level criterion into components or aspects that are fundamental to it. The criterion of crop mulch (First level) was created considering that crop mulch requires two criteria to be assessed: - the mean of crop mulch on the strip and its variability (coefficient of variation of coverage of the strip) (Second level criteria). Similarly, the First level criterion of harvested waste was created considering that the crop mulch after the harvest also requires two criteria to be assessed: the mean and the variability of crop mulch (Second level criteria).

Spacing between lines was also assessed by two Second level criteria: - mean and variability of the spacing (coefficient of variation of the spacing on the strip).

In assessment of the plant distribution criterion (First level), three Second level criteria were used: properly spaced plants and their density, number of spaces between plants greater than 0.10 m and smaller than 0.30 m, and length of gaps (length of gaps greater than 0.30 m).

The infestation of weeds (First level criterion) was assessed considering infestation during the development phase and after the harvest.

For the criteria of date of sowing and loss of crop, no sub-criteria were created and their means were used directly as First level criteria.

Forman and Selly (2001) consider that the AHP (Analytic Hierarchy Process) is based on three fundamental principles: decomposition, comparative judgement and hierarchical composition. With the criteria arranged into a hierarchy, the comparative judgment among the criteria of one same level is done through the construction of a square matrix with the values of the important rates to be compared among the criteria. The rate of importance among the criteria is established according to a scale of values ranging from 1/9 to 9.

Once the matrix is constructed, the weightings of each criterion were extracted by the Weight model of IDRISI 32, which calculates the principal autovector by producing weightings that added together are equal to One (1), as required by the procedure of weight linear combination (WLC).

OPERATIONAL QUALITY

Operational quality is the objective that seeks to assess the quality of the performed operations. Considering the large number of criteria at the First level and the importance of the matrix consistency, Table I was drawn up with a verbal scale which places the First level criteria in a hierarchy, starting from the most important criterion. This table assisted in making an assessment of consistency by comparison of parity. On making the comparison of parity, when there was any disagreement with the previously established matrix, the table was re-scaled in order to represent correctly the relative importance of the criteria. The foundation of the process was the development of coherent argumentation that would allow a determination of the value of relative importance for the criteria.

The relative importance given to the Second level criteria and the information reliability on criterion assessment were both taken into account regarding the establishment of the matrix rates of importance for estimating these criteria.

FIRST LEVEL CRITERIA FOR OPERATIONAL QUALITY

The First level criteria chosen for the determination of operational quality were Crop mulch, Spacing among lines, Distribution of plants in the line of sowing, Infestation of weeds, Date of sowing, Harvested waste, Loss of crop.

- Crop mulch was considered a highly important criterion for the development of the plants in the assessment of operational quality, as it is influenced by many operations, mainly by the harvest, preparation of the soil and sowing. This index is considered by the agronomic community an excellent means of assessment of operational quality. The method of determining this variable was considered highly reliable.

- Spacing among lines was considered a very important criterion in the assessment of operational quality of the crops, since it assesses directly the quality of the sowing operation. In this case, variability is far more important than the mean spacing. Whereas variability is linked to operational failures, mean spacing is more a function of the farmer's choice. Its lack of uniformity (variability) may mean the loss of plants due to crushing during care of the crop, with an increase of losses in the harvest.

- The distribution of plants in the line of sowing was considered a highly important criterion in assessment of the operational quality of the crops, as it assesses directly the quality of the sowing, which is the most complex and delicate operation in setting up a corn plantation. The method of determination was established by the integration of three sub-criteria (density, number of proper spaces, length of gaps).

- The infestation of weeds in the crop is a very important criterion in assessment of operational quality, as it assesses the operations of control and handling of the weeds. The infestation of weeds was assessed at two points, namely: during crop development and post harvest. Each point was considered as a Second level criterion. Reliability of the information is good.

- The date of sowing is a highly important criterion in the indirect measurement of operational quality. Being determined on the basis of the date of the after-harvest sampling, it displays poor reliability. However, it is a criterion that assesses the planning and suitability of the time of starting the plantation.

- Harvested waste (crop mulch after the harvest) was considered a highly important criterion in the assessment of operational quality, as it assesses the quality of the performed harvest. This variable assesses the system of crushing and distribution of straw by the harvester.

- Loss of crop was considered a highly important criterion in assessment of operational quality and it was measured as for the Harvested waste. On top of this, it assesses indirectly a whole series of operational conditions (suitability of the time of harvest, distribution of plants, health of the crop) which causes plants to fall over and ears to drop off, among other factors.

SECOND LEVEL CRITERIA FOR OPERATIONAL QUALITY

The mean of crop mulch (Second level criterion) was considered moderately less important than the variability of crop mulch in the determination of crop mulch during development and in harvested waste (coverage after harvest), which is a First level criterion in the assessment of operational quality. We took into account that variability is the most important factor in the determination of operational quality, as this is related to the uniformity of the operations that influenced the distribution and uniformity of this coverage, whereas the means are far more dependent on the productivity of previous crops.

The variability of spacing among the lines (Second level criterion) was the only factor considered in determining the spaces among the lines (First level criterion in the assessment of operational quality). The mean of spacing was disregarded as a criterion for assessment of operational quality, since the mean depends only on the spacing option chosen by the farmer.

In the determination of plant distribution (First level criterion), the number of suitable spaces among plants was considered moderately more important than density and the length of gaps. it was considered that, among the assessed criteria, the number of suitable spaces among plants better assesses the quality of sowing than density and the length of gaps.

The infestation of weeds after harvest (Second level criterion) was considered moderately less important than the infestation of weeds during the development of the crop in determining the infestation of weeds, a First level criterion in the assessment of operational quality. Whereas the infestation of weeds during development assesses the quality of operations of weed control, the infestation of weeds after harvest influences the quality of the harvest, but does not assess it directly, being rather a function of closing and competition for water, light and nutrients of the crop and the bank of seeds in the soil, and may be the outcome of a late emergence of weeds.

NORMALIZATION OF THE VALUES OF THE CRITERIA

To make it possible to compare and combine information from different criteria, it was necessary to transform the values of the criteria into one selfsame index of assessment. in the case of Boolean criteria, the levels of information are transformed into values between 0 and 1, and thus they can be combined. However, continuous factors need to be combined through the sum total of the weightings multiplied by normalized values of the criteria (Eastman 2001).

in which: S = suitability; wi = weighting of factor i; and xi = normalized value of the criterion of factor i.

Fuzzy logic was then employed for normalizing the factors using the functions of pertinence. The use of fuzzy logic was performed in Idrisi 32.

DEFINITIONS OFCUT-OFFVALUES FOR CALCULATING THE VALUES OF PERTINENCE ANDESTABLISHING THEFUZZYSET ON THEBASIS OF SETS OF SUPPORTING VALUES FOR THE CRITERIA

The procedure adopted sought to establish the cut-off values and the interval of values using the statistics of the population studied. In this way, the mean of the population always represents the degree of pertinence 0.5 for monotone functions of pertinence and a value of one (1) for symmetrical functions, and the limits used for determining the inflexion values were established placing the degree of pertinence of the fuzzy set [0.1] among x values that would represent almost the whole sampled population, preserving a symmetry of function around the mean.

RESULTS AND DISCUSSION

WEIGHTINGS FOR THE FIRST LEVEL CRITERIA IN THE DETERMINATION OF OPERATIONAL QUALITY

Table II presents the matrix of parity comparison of relative importance of the First level criteria, the basis to calculate weightings that settle the operational quality of the crops (objective).

The weightings which were calculated on the basis of the above matrix by the method of normalized autovector (Forman and Selly 2001) were crop mulch, 0.1526; spacing between lines, 0.0788; plant distribution, 0.3605; infestation of weeds, 0.1134; date of sowing, 0.0446; harvested waste, 0.0552; and loss of harvest, 0.1958. The rate of consistency, 0.04, indicates coherence in the values of the rates of importance that made up the matrix.

The method (Forman and Selly 2001) allowed the establishment of the matrix set out in Table III which, in its turn, allowed calculation of the presented weightings. The index of consistency of 0.013 and a rate of consistency of 0.014 indicate coherence in the values of the rates of importance that made up the matrix.

NORMALIZATION OF CRITERIA FOR ASSESSMENT OF OPERATIONAL QUALITY

Table IV presents the chosen functions of pertinence, the forms of these functions and the inflexion values employed in the normalization of each First or Second level criterion in the assessment of operational quality.

As already described, the values of the points of inflexion, for the case of operational quality, were determined on the basis of the statistics of each variable.

MULTI-CRITERION ANALYSIS OF OPERATIONAL QUALITY

Figure 4 presents the spatial distribution of operational quality of the crops on a scale where zero (0) represents the planted strip of worst operational quality and 255 the strip of best operational quality. One finds the existence of a nucleus of poor quality of crops to the North of the Municipality of Tarumã (Southwest of Assis, Southeast of Maracai) which probably reflects the dispersed and fragmented crops in a region dominated by sugar cane, more sandy soils and the lack of technical assistance from the grain cooperatives settled in Cândido Mota (Coopermota) and Pedrinhas Paulista (CAP).


On the other hand, a clear nucleus of quality can be seen to the Southwest of Cândido Mota, a region of medium and large producers, with a good level of capitalization and high technology. These communities called Água do Macuco and lower Pari enjoy considerable action from the assistance of Coopermota and sales of agricultural inputs.

A band running East-West contains crops of high operational quality, and is precisely on the axis of operations of the two most important cooperatives in the region; CAP (Pedrinhas Paulista Agricultural Cooperative) and Coopermota (Cooperative of Coffee Growers of the Mid Sorocabana Region).

The fall-off in quality close to the Paranapanema River is due to the poor levels of quality of the crops assessed in the North of Paraná State which, while not mapped, influenced values to the North of the river, on the São Paulo side.

The weightings of the autovector are listed in Table V, in which the rate of consistency was 0.04.

From the standpoint of the influence of the criteria in the make-up of the map of operational quality (Graph 1), we discovered the great influence of plant distribution (36% of weighting), which has, as its principal sub-criterion, the number of suitably spaced plants. Besides this criterion, the loss of crop, with a weighting of approximately 20%, and crop mulch, with 15% weighting, also had a strong impact on the values of the map of operational quality.


CONCLUSIONS

The available items of verification allowed classification of the operational quality of the crops and the development of a method that makes possible, in subsequent works, analysis and mapping of a more general quality of Agricultural Operations.

The strategy of multi-criterion analysis and Fuzzy logic proved to be important tools for the assessment and preparation of a quality index for corn cultivation.

The index was good in representing the quality of agricultural operations when compared with reality.

Manuscript received on July 7, 2008; accepted for publication on March 3, 2009; presented by ELIBIOL. RECH

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  • Correspondence to:

    Rubens Augusto Camargo Lamparelli
    E-mail:
  • Publication Dates

    • Publication in this collection
      16 Nov 2009
    • Date of issue
      Dec 2009

    History

    • Accepted
      03 Mar 2009
    • Received
      07 July 2008
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