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• Arq. Neuro-Psiquiatr. 18 (1) • Mar 1960 • https://doi.org/10.1590/S0004-282X1960000100002   copiar

Electroforese em papel das proteínas do líqüido cefalorraquidiano: IV. valores normais

Paper strip electrophoresis of cerebrospinal fluid proteins: IV. normal values

Autoria SCIMAGO INSTITUTIONS RANKINGS

Resumos

Foram analisadas, mediante electroforese em papel, as proteínas do LCR cisternal de 30 pessoas adultas (13 sadias e 17 portadoras de neuroses); as médias encontradas em relação às diversas frações proteicas foram as seguintes: pré-albumina 2,2%; albumina 51,6%; globulinas α1 5,0%, α2 8,7%, β (incluindo os percentuais da fração τ) 21,6% e γ 10,9%. Em relação aos resultados encontrados para as frações proteicas do soro sangüíneo de 30 pessoas adultas (17 normais e 13 portadoras de neuroses) incluindo 7 daquelas cujo LCR foi estudado, o perfil das proteínas do LCR mostrou-se diferente, pois no LCR verifica-se a presença da fração pré-albumina, maior riqueza em globulinas β e pequeno teor de γ-globulina.

Cisternal cerebrospinal fluid (CSF) protein fractions were studied by paper electrophoresis. Through sub-occipital puncture samples were collected from 30 adults (age range from 15 to 45 years old) : 13 healthy people and 17 psychoneurotic patients, without past history of neurologic disease and/or evidence of organic affection. Syphilis serology was negative in all cases as well as CSF showed any abnormality in respect to pressure, aspect, color, cytology, total protein content, colloidal reactions, syphilis and cysti-cercosis complement fixation tests. Technical details were previously described; protein concentration was achieved by dyalisis against 30% poli-vinylpirrolidone at 4°C. Results are detailed in the table 1; mean values obtained for protein fractions were: pre-albumin 2.2%, albumin 51.6%, globulins: α1 5.0%, α2 8.7%, β 21.6% (including the τ fraction) and γ 10.9%. There was any significative difference between results found in normal people and in psychoneurotic patients. The values found are in agreement with those reported by Mumenthaler and Marki from data published until 1956. For comparison purposes the author's data are presented together with others obtained by several investigators (table 4). The results were compared to those found in sera from 30 adults selected in the same way and analysed by the same technique (table 2). Comparison showed similar results when the means found in respect to all sera and CSF samples were considered or when means found for seven cases in which it was possible to analyse CSF and serum proteins simultaneously were considered (table 3). Albumin (including pre-albumin) and α-globulins have similar relative patterns in both CSF and serum protein profiles, but those of β and y-globulins are disimilar. As far as to the sera, CSF proteins have relatively more jg-globulin (1.5 to twices) and less y-globulin (0.5 approximately).

Electroforese em papel das proteínas do líqüido cefalorraquidiano: IV. valores normais

Paper strip electrophoresis of cerebrospinal fluid proteins. IV. normal values

A. Spina-França

Assistente extranumerário. Laboratório da Clínica Neurológica da Fac. Med. da Univ. de São Paulo (Prof. Adherbal Tolosa)

RESUMO

Foram analisadas, mediante electroforese em papel, as proteínas do LCR cisternal de 30 pessoas adultas (13 sadias e 17 portadoras de neuroses); as médias encontradas em relação às diversas frações proteicas foram as seguintes: pré-albumina 2,2%; albumina 51,6%; globulinas α1 5,0%, α2 8,7%, β (incluindo os percentuais da fração τ) 21,6% e γ 10,9%.

Em relação aos resultados encontrados para as frações proteicas do soro sangüíneo de 30 pessoas adultas (17 normais e 13 portadoras de neuroses) incluindo 7 daquelas cujo LCR foi estudado, o perfil das proteínas do LCR mostrou-se diferente, pois no LCR verifica-se a presença da fração pré-albumina, maior riqueza em globulinas β e pequeno teor de γ-globulina.

SUMMARY

Cisternal cerebrospinal fluid (CSF) protein fractions were studied by paper electrophoresis. Through sub-occipital puncture samples were collected from 30 adults (age range from 15 to 45 years old) : 13 healthy people and 17 psychoneurotic patients, without past history of neurologic disease and/or evidence of organic affection. Syphilis serology was negative in all cases as well as CSF showed any abnormality in respect to pressure, aspect, color, cytology, total protein content, colloidal reactions, syphilis and cysti-cercosis complement fixation tests. Technical details were previously described; protein concentration was achieved by dyalisis against 30% poli-vinylpirrolidone at 4°C.

Results are detailed in the table 1; mean values obtained for protein fractions were: pre-albumin 2.2%, albumin 51.6%, globulins: α1 5.0%, α2 8.7%, β 21.6% (including the τ fraction) and γ 10.9%. There was any significative difference between results found in normal people and in psychoneurotic patients. The values found are in agreement with those reported by Mumenthaler and Marki from data published until 1956. For comparison purposes the author's data are presented together with others obtained by several investigators (table 4).

The results were compared to those found in sera from 30 adults selected in the same way and analysed by the same technique (table 2). Comparison showed similar results when the means found in respect to all sera and CSF samples were considered or when means found for seven cases in which it was possible to analyse CSF and serum proteins simultaneously were considered (table 3). Albumin (including pre-albumin) and α-globulins have similar relative patterns in both CSF and serum protein profiles, but those of β and y-globulins are disimilar. As far as to the sera, CSF proteins have relatively more jg-globulin (1.5 to twices) and less y-globulin (0.5 approximately).

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REFERÊNCIAS

1. BAUER, H. - Über die Bedeutung der Papier Elektrophorese des Liquors für die klinische Forschung. Dtsch. Z. Nervenh., 170:381 (setembro) 1953.

2. BÉLANGER, C; MARTIN, J. L. - Études sur le liquide céphalo-rachidien par l'électro-phorèse sur papier. I. Méthodes et valeurs normales. Laval Méd., 26:622 (novembro) 1958.

3. DELANK, H. W. - Klinische Erfahrungen mit elektrophoretischen Liquoreiweissuntersuchungen. Dtsch. Z. Nervenh., 174:429 (abril) 1956.

4. ESSER, H. - Die elektrophoretische Untersuchung der Liquoreiweisskörper und ihre klinische Bedeutung. Münch, med. Wschr., 94:2313 (novembro, 14) 1952.

5. HANZAL, F.; VYMAZAL, J. - The significance of electrophoretic investigation in inflammatory diseases of the nervous system. Acta Psychiat. Neurol. Scand., 33:283, 1958.

6. HAUSTIN, J. - Prefácio ao livro de Sunderman, F. W. e Boerner, F. - Normal Values in Clinical Medicine. Ed. W. B. Saunders Co., Filadélfia, 1950.

7. KNAPP, A. - Über die Papierelektrophorese des Liquor cerebrospinalis. Arch. klin. experiment. Dermat., 201:446, 1955.

8. KUTZIM, H.; SCHEID, W.; VONKENNEL, J. - Die Verschiebung der Bluteiweisskörper im Blut und Liquor bei Syphilis des Zentralnervensystem. Medizinische, 17:609 (abril, 24) 1954.

9. MUMENTHALER, M.; MÄRKI, H. - über die Liquorelektrophorese. Methodik und klinische Anwendung. Klin. Wschr., 35:1 (janeiro, 1) 1957.

10. OLDERSHAUSEN, H. F. v.; GRIES, G.; ALY, F. W. - Zur klinischen Bedeutung von Liquor und Serumelektrophoreseuntersuchungen bei der Poliomyelitis anterior acuta. Dtsch. Z. Nervenh., 170:254 (junho) 1953.

11. RISIO, C. De; INESI, G.; TONINI, G. - Studies of the proteins of human cerebrospinal fluid by paper electrophoresis. Confinia Neurol., 17:271, 1957.

12. ROSSI, R. - L'elettroforesi nello studio delle frazloni proteiche, lipoproteiche e glicoproteiche del liquor normale ed in corso di meningite tubercolare. Riv. Clin. Pediat. (Florenca) 55:426 (maio) 1957.

13. SARTESCHI, P.; FABIANI, P. - Limiti all'indagine elettroforetica su carta della crasi proteica ematoliquorali in malatti neurologici e psichici. Rass. St. Psichiat., 45:1193 (novembro-dezembro) 1956.

14. SCHMIDT, C; MATIAR, H. - Das quantitative Verhältnis der Serum und Liquorproteine. Dtsch. Z. Nervenh., 174:443 (abril) 1956.

15. SCHÖNENBERG, H. - Die Papier Elektrophorese des Liquor cerebrospinalis. Ann. Paediat. (Basiléia), 183:309 (novembro) 1954.

16. SPINA-FRANÇA, A. - Electroforese em papel das proteínas do liqüido cefalorraquidiano: a) I. Considerações gerais sôbre a electroforese em papel. Arq. Neuro-Psiquiat., 16:155 (junho) 1958. b) II. Principais resultados registrados na literatura. Arq. Neuro-Psiquiat., 16:223 (setembro) 1958. c) III. Técnica. Arq. Neuro-Psiquiat, 16:236 (setembro) 1958.

17. TANAKA, Z. - Electrophoretic study of cerebrospinal fluid protein components. I. Survey in normal liquor. Tohoku J. Exper. Med. (Sendai), 63:245 (fevereiro) 1956.

18. WIDELL, S. - On the cerebrospinal fluid in normal children and in patients with acute abacterical meningoencephalitis. Acta Paediat. (Uppsala), supl. 115, maio 1958.

Clínica Neurológica. Hospital das Clínicas da Fac. Med. da Univ. de São Paulo - Caixa Postal 3461 - São Paulo, Brasil.

  • 1. BAUER, H. - Über die Bedeutung der Papier Elektrophorese des Liquors für die klinische Forschung. Dtsch. Z. Nervenh., 170:381 (setembro) 1953.
  • 2. BÉLANGER, C; MARTIN, J. L. - Études sur le liquide céphalo-rachidien par l'électro-phorèse sur papier. I. Méthodes et valeurs normales. Laval Méd., 26:622 (novembro) 1958.
  • 3. DELANK, H. W. - Klinische Erfahrungen mit elektrophoretischen Liquoreiweissuntersuchungen. Dtsch. Z. Nervenh., 174:429 (abril) 1956.
  • 4. ESSER, H. - Die elektrophoretische Untersuchung der Liquoreiweisskörper und ihre klinische Bedeutung. Münch, med. Wschr., 94:2313 (novembro, 14) 1952.
  • 5. HANZAL, F.; VYMAZAL, J. - The significance of electrophoretic investigation in inflammatory diseases of the nervous system. Acta Psychiat. Neurol. Scand., 33:283, 1958.
  • 6. HAUSTIN, J. - Prefácio ao livro de Sunderman, F. W. e Boerner, F. - Normal Values in Clinical Medicine. Ed. W. B. Saunders Co., Filadélfia, 1950.
  • 7. KNAPP, A. - Über die Papierelektrophorese des Liquor cerebrospinalis. Arch. klin. experiment. Dermat., 201:446, 1955.
  • 8. KUTZIM, H.; SCHEID, W.; VONKENNEL, J. - Die Verschiebung der Bluteiweisskörper im Blut und Liquor bei Syphilis des Zentralnervensystem. Medizinische, 17:609 (abril, 24) 1954.
  • 9. MUMENTHALER, M.; MÄRKI, H. - über die Liquorelektrophorese. Methodik und klinische Anwendung. Klin. Wschr., 35:1 (janeiro, 1) 1957.
  • 10. OLDERSHAUSEN, H. F. v.; GRIES, G.; ALY, F. W. - Zur klinischen Bedeutung von Liquor und Serumelektrophoreseuntersuchungen bei der Poliomyelitis anterior acuta. Dtsch. Z. Nervenh., 170:254 (junho) 1953.
  • 11. RISIO, C. De; INESI, G.; TONINI, G. - Studies of the proteins of human cerebrospinal fluid by paper electrophoresis. Confinia Neurol., 17:271, 1957.
  • 12. ROSSI, R. - L'elettroforesi nello studio delle frazloni proteiche, lipoproteiche e glicoproteiche del liquor normale ed in corso di meningite tubercolare. Riv. Clin. Pediat. (Florenca) 55:426 (maio) 1957.
  • 13. SARTESCHI, P.; FABIANI, P. - Limiti all'indagine elettroforetica su carta della crasi proteica ematoliquorali in malatti neurologici e psichici. Rass. St. Psichiat., 45:1193 (novembro-dezembro) 1956.
  • 14. SCHMIDT, C; MATIAR, H. - Das quantitative Verhältnis der Serum und Liquorproteine. Dtsch. Z. Nervenh., 174:443 (abril) 1956.
  • 15. SCHÖNENBERG, H. - Die Papier Elektrophorese des Liquor cerebrospinalis. Ann. Paediat. (Basiléia), 183:309 (novembro) 1954.
  • 16. SPINA-FRANÇA, A. - Electroforese em papel das proteínas do liqüido cefalorraquidiano: a) I. Considerações gerais sôbre a electroforese em papel. Arq. Neuro-Psiquiat., 16:155 (junho) 1958.
  • b) II. Principais resultados registrados na literatura. Arq. Neuro-Psiquiat., 16:223 (setembro) 1958.
  • c) III. Técnica. Arq. Neuro-Psiquiat, 16:236 (setembro) 1958.
  • 17. TANAKA, Z. - Electrophoretic study of cerebrospinal fluid protein components. I. Survey in normal liquor. Tohoku J. Exper. Med. (Sendai), 63:245 (fevereiro) 1956.
  • 18. WIDELL, S. - On the cerebrospinal fluid in normal children and in patients with acute abacterical meningoencephalitis. Acta Paediat. (Uppsala), supl. 115, maio 1958.

Datas de Publicação

  • Publicação nesta coleção
    09 Dez 2013
  • Data do Fascículo
    Mar 1960
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