The enantioselective synthesis of (R)-(+)-6-isopropenyl-3-methyl-2-cycloheptenone

ARTICLE

The enantioselective synthesis of (R)-(+)-6-isopropenyl-3-methyl-2-cycloheptenone

Timothy J. Brocksom^*; Ursula Brocksom; Fernanda P. Barbosa

Departamento de Química, Universidade Federal de São Carlos, 13565-905 São Carlos-SP, Brazil

ABSTRACT

(R)-(+)-6-isopropenyl-3-methyl-2-cycloheptenone is synthesized from (R)-(-)-carvone by reduction and trialkylsilyl-enolether formation, cyclopropanation with methylene iodide and diethylzinc, followed by a Saegusa type oxidation with ferric chloride and finally dehydrochlorination with base. The title compound is obtained in five steps and 38% overall yield, being a useful chiron for guaiane sesquiterpene natural products.

Keywords: cycloheptenone, carvone, ring expansion, perhydroazulene, enantioselectivity

RESUMO

(R)-(+)-6-isopropenil-3-metil-2-cicloeptenona foi sintetizada a partir de (R)-(-)-carvona, por redução e formação do éter-enólico-trialquilsilil, ciclopropanação com iodeto de metileno e dietil-zinco, seguida por oxidação de Saegusa com cloreto férrico e finalmente, desidroalogenação com base. O produto final, um quiron para a síntese de produtos naturais sesquiterpênicos do tipo guaiano, foi obtido em cinco etapas com 38% de rendimento global.

INTRODUCTION

The total synthesis of guaiane sesquiterpenes¹ is of current interest due to innumerous examples of these natural products with relevant biological activities, and the challenge of the perhydroazulene carbon skeleton containing two methyl and an isopropyl groups. Our interest in their synthesis has led us to develop methods for the transformation of para-menthane monoterpenes into single isomer cycloheptenones,^2-4 and then by pent-annelation^5,6 into the required complete carbon skeleton. Scheme 1 outlines our synthetic strategy which involves two quite different routes for the ring expansion, by cyclopropanation and central sigma bond cleavage^2,3 or nucleophilic addition and rearrangement.⁴ The intermediate cycloheptenone can then by pent-annelated by more classical ionic reactions (allylation, Wacker oxidation and intramolecular aldol reaction)⁵ or by the ring closing metathesis⁶ of a suitable neighboring allyl-vinyl derivative.

Thus far we have developed several distinct methodologies for the ring expansion of different para-menthane monoterpenes, and in this paper we would like to describe a new route which transforms readily available (R)-(-)-carvone (1) into the desired (R)-(+)-6-isopropenyl-3-methyl-2-cycloheptenone (2) in five steps and 38% overall yield (Scheme 2), with complete regioselectivity and retention of stereochemical integrity.

Results and Discussion

The enantiodivergent strategy we have developed³ requires a double bond in the para-menthane structure at the C-1 to C-2 centers for the (R)-cycloheptenone enantiomer or the C-1 to C-6 centers for the (S)-enantiomer (Scheme 3).

Herein we describe an enantio- and regioselective route to the (R)-enantiomer of 2 by regioselective formation of the trialkylsilyl-enolether 4 of dihydrocarvone (3) followed by chemoselective cyclopropanation and oxidative cleavage of the common sigma bond. Elimination of HCl with base completes the synthesis of 2 (Scheme 4).

A similar strategy was used by Marques et al.⁷ in the synthesis of the sesquiterpene africanol, for the ring expansion of 2,5,5-trimethyl-cyclohexanone.

The reduction of the conjugated double bond of (R)-(-)-carvone (1) can be readily effected with zinc dust in alkaline methanol/water as we have developed³ in our laboratory, as a simple modification of the well known electron transfer methods, thus producing dihydrocarvone (3) as an epimeric mixture. The weak base catalyzed enolization leads to the thermodynamically preferred enolate which is captured by both trimethylsilyl chloride and triethylsilyl chloride, leading to enolethers 4a and 4b in excellent overall yields from carvone (1).³

The key step of chemoselective cyclopropanation of 4 to produce the substituted nor-carane 5 was executed by the Furukawa modification⁸ of the Simmons-Smith reaction,⁹ using methylene iodide in the presence of diethylzinc. This reaction was found to be very capricious with respect to reproducibility and highly influenced by the two reagents. Methylene iodide is not easily purified by standard methods including distillation and chromatography on silica gel without substantial loss of reagent. In the event, we purify methylene iodide by quick treatment with the minimum quantity of silica gel whereupon the reagent becomes colorless for a short time. In a similar fashion, solutions of diethylzinc are of variable reactivity depending upon concentration, solvent and even batch, and diethylzinc is best acquired as a dilute solution (approximately molar) in hexane.

We have found very little difference between the reactivities of the trimethylsilyl ether 4a or the triethylsilyl ether 4b, the same being true for their stabilities with respect to the reagents. Thus the cyclopropanation reactions described below were all performed with the more readily available trimethylsilyl ether 4a.

In the first procedure described in the experimental section, we have utilized the very surprising catalytic effect of a dry oxygen atmosphere¹⁰ on this cyclopropanation reaction. This method gave us the best results of 83% yield of 5 and 6 in a 95 to 5 relation. This reaction was performed in toluene and dichloromethane (1:1) for 2 hours at room temperature with one equivalent of methylene iodide. Gas chromatographic analysis of the diastereomeric mixture of 5 gave a 6% diastereomeric excess of an undetermined isomer, confirming the expected lack of stereoselectivity of the cyclopropanation at the internal double bond.

The standard procedure⁸ with maintenance of the dry nitrogen atmosphere and conducted in toluene as solvent, gave variable results of chemoselectivity and isolated crude yields of 5 and 6, as described in the second procedure. Although higher yields can be obtained with the use of more methylene iodide and diethyl zinc, as expected the chemoselectivity for 5 over 6 decreases substantially. A third method was attempted based on a publication by Charette et al.¹¹ In this method the solvent is dimethoxyethane which is proposed to have a complexing effect on the organo-zinc carbenoid. In the event, and with large excesses of methylene iodide and diethylzinc in dichloromethane for extended reaction times, no reaction was observed and starting material was recovered.

The separation and purification of 5 and 6 is not trivial, and requires careful column chromatography. We obviously investigated many different reaction conditions to avoid formation of the bis-cyclopropane 6 but with no success, which indicates similar kinetics of the reactions at the two double bonds. Although we had expected much higher reactivity at the trialkylsilyl-enol ether site for electronic arguments, we interpret this as being due to the reactivity of the methylene zinc carbenoid and possible steric effects. However, the cyclopropanation reaction is still highly chemoselective for the desired double bond, but the bis-cyclopropanated product 6 is still present to the extent of 5% in subsequent reactions.

The Saegusa oxidation¹² of mono-cyclopropanated 5 with dry ferric chloride in dimethylformamide led easily to the ring expanded 3-epimeric mixture of the 3-chloro-cycloheptanone 7 in 82% yield. Finally, treatment with sodium acetate in methanol at reflux furnished the conjugated cycloheptenone 2 in 74% yield. Alternatively, we have executed these two reactions without isolation of intermediate 7, although the yield is comparable, and this procedure is also described in the experimental section.

Gas chromatographic analyses on chiral columns were performed, and demonstrate unequivocally that the cycloheptenone 2 has the same extremely high enantiomeric excess as starting material (R)-(-)-carvone (1), and therefore maintenance of stereochemical integrity. Determination of the optical activity allowed comparison with samples prepared earlier in our laboratory³ and also confirm very high enantiomeric excess. The previous samples^3,4 have optical rotations (a_D) of +51.0 (c 1.47, (CHCl₃) and +49.0 (c 0.13, CHCl₃) respectively, whereas the present work shows optical rotations of +61.3 and +56.0 (c 0.02, CHCl₃) in two different procedures. These small differences are certainly due to the presence of trace chemical impurities and not to different enantiomeric excesses.

Conclusions

We have demonstrated the efficient synthesis of the single isomer (R)-(+)-cycloheptenone 2 in five steps from (R)-(-)-carvone (1). With the exception of the cyclopropanation reaction, the other reactions are all easily amenable to execution at the 10 to 20 gram scale, use relatively simple reagents and require very little chromatographic purification. This allows us to prepare large quantities of the desired cycloheptenone 2 in a relatively short time.

Experimental

General

¹H and ¹³C NMR spectra were obtained on a Bruker DRX-400 spectrometer at 400 and 100 MHz, and on a Bruker ARX-200 spectrometer at 200 and 50 MHz respectively, with CDCl₃ as solvent. Chemical shifts are in ppm downfield from a tetramethylsilane internal standard. Infrared spectra were recorded on Bomen Michelson model 102 FTIR or Hartman & Braun MB, and the most intense or representative bands are reported (in cm^-1). Mass spectra were determined by APCI with MeOH using a Micromass Quattro LC/MS spectrometer. Melting points were determined on a Micro Química model APF 301 apparatus and are uncorrected. Radial chromatography was performed on a Chromatotron^® model 8924 with 2 mm plates of silica gel 60, PF 254 with calcium sulphate, E. Merck 7749. Gas liquid chromatographic analyses were performed on a Shimadzu GC-17A, equipped with a DB-1 capillary column (0.25 mm x 30 m) and using nitrogen as carrier gas. The chiral GC analyses were performed on a heptakis-2,6-dimethyl-3-pentyl-b-cyclodextrin column on a HP Agilent 6890 series gas chromatograph, with nitrogen as carrier gas, at the Chemistry Institute, UNICAMP, in Campinas. The optical rotation measurements were determined on a Perkin-Elmer model 241 polarimeter. The micro-analytical data were obtained on a Fisons EA 1108 CHNS-O Analyser. Reactions with compounds sensitive to air or moisture were performed under a dry nitrogen atmosphere. Solvents and reagents were used directly from the manufacturer, or purified when required by standard procedures. Anhydrous solvents were dried by refluxing over sodium wire in the presence of the benzophenone ketyl as indicator. Methylene iodide was purified by swirling in a conical flask with the minimum quantity of chromatographic quality silica gel, and decantation under nitrogen pressure. Diethylzinc is an approximately 1 molar solution in hexane, obtained from Aldrich, and analyzed previous to use by the usual methodology for butyllithium solutions.

Dihydrocarvone (3), the trimethylsilyl-enolether 4a and the triethylsilyl-enolether 4b were prepared by our published procedures.³

Cyclopropanation of the trimethylsilyl-enolether 4a to (3R)-3-isopropenyl-6-methyl-1-trimethylsilyloxy-bicyclo -[4.1.0.]-heptane 5

Method (i). To a solution of trimethylsilyl-enolether 4a (1.413 g, 6.29 mmol) in dry toluene and CH₂Cl₂ (1:1; 6.30 mL), under dry nitrogen and at ice bath temperature, was slowly added a solution of Et₂Zn (1.0 mol L^-1 in hexane, 6.30 mL, 6.30 mmol), and then drop-by-drop CH₂I₂ (0.30 mL, 6.30 mmol). The inert atmosphere and ice bath were removed and the reaction mixture was stirred under a dry oxygen atmosphere. After 2 h, the mixture was stirred with a saturated solution of NH₄Cl (8 mL) for 10 min. The suspension was filtered through Celite^® with a hexane wash, the organic layer was then washed with a solution of NH₄Cl and NH₄OH (95:0.5) and a saturated solution of NH₄Cl, dried over anhydrous Na₂SO₄, filtered, and the solvent was evaporated. The crude product (1.494 g) was purified by column chromatography on silica gel, using hexane with 2% of Et₃N as eluent, to afford 1.235 g (83%) of 5 as a yellow oil; [a]_D²⁹ +54.0 (c=0.03; CHCl₃).

An analytical sample was prepared by further purification by column chromatography on silica gel, using hexane: EtOAc (98:2) with 2% of Et₃N as eluent. IR n_max/cm^-1: 840, 983, 1250,1447,1691,2855, 2925, 3068. ¹H NMR (400MHz, CDCl₃) d: 0.15 (broad s, 9H), 0.39-0.46 (m, 2H), 1.18 (s, 3H), 1.67 (s, 3H), 2.13-2.18 (m, 1H), 4.63-4.65 (m, 2H); ¹³C NMR (50 MHz, CDCl₃) d 1.4, 20.7, 20.8, 23.8, 25.7, 28.1, 31.7, 40.9, 42.1, 60.2, 108.5, 149.9. MS (APCI), m/z [M+H]⁺= 239. [a]_D²⁷ + 70.5 (c = 0.42; CHCl₃). Anal. Calc. for C₁₄H₂₆SiO: C, 70.52; H, 10.99: Found: C, 70.33; H, 10.75.

Method (ii). To a solution of trimethylsilyl-enolether 4a (571 mg, 2.54 mmol) in anhydrous toluene (3.28 mL), under dry nitrogen at aprox. –10 ºC, was slowly added a solution of Et₂Zn (1.0 mol L^-1 in hexane, 4.58 mL, 4.58 mmol), and then drop-by-drop CH₂I₂ (0.28 mL, 3.52 mmol, 1.38 equiv.). The cooling bath was removed after 15 min, and the reaction was followed by gas chromatographic analysis. The reaction mixture was stirred at room temperature for 2.5 h, and then a saturated solution of NH₄Cl (2 mL) was added and allowed to stir for 10 min. The suspension was filtered through Celite^® and washed with hexane, the organic layer was then washed with a saturated solution of NH₄Cl, dried over anhydrous Na₂SO₄, filtered, and the solvent evaporated to give the crude product (589 mg). GC analysis indicated the presence of 20% starting material 4a, and products 5 and 6 in a 4 to 1 proportion, as determined by peak areas.

(6R)-3-chloro-6-isopropenyl-3-methyl-cycloheptanone (7)

Method (i). To a solution of 5 (589 mg, with traces of 6) in DMF (2.10 mL) and CH₂Cl₂ (0.10 mL) at O ºC, was added dropwise a solution of FeCl₃ (1.160 g, 7.14 mmol; purified by refluxing with SOCl₂) in DMF (4.60 mL). The reaction mixture was stirred for 1 h, at O ºC and another 2 h at room temperature. A solution of HCl (1.0 mol L^-1, 10 mL) was then added and the product was extracted with hexane (7 x 10 mL). The organic layer was washed successively with a solution of HCl (1.0 mol L^-1, 10 mL), a saturated solution of NaHCO₃, and brine, dried over anhydrous Na₂SO₄, filtered, and the solvent was evaporated to give 7 (407 mg, 82%).

Method (ii). To a solution of 5 (1.235 g, with traces of 6) in THF (0.30 mL) and CH₂Cl₂ (6.35 mL), at O ºC and under a nitrogen atmosphere, was added drop-by-drop a suspension of anhydrous FeCl₃ (3.30 g, 20.31 mmol) and silica gel (3.30 g) in THF (13.50 mL). After 1 h the cooling bath was removed, and the reaction mixture was stirred at room temperature for 2 h. The nitrogen atmosphere was removed and the reaction mixture was treated directly as described in procedure (ii) below.

(6R)-6-isopropenyl-3-methyl-2-cycloheptenone (2)

Method (i). A solution of 7 (483 mg) in MeOH (30 mL) and a saturated solution of NaOAc (10.0 mL) in MeOH (30 mL) was stirred and the MeOH was evaporated. To the reaction mixture was added MeOH (30 mL), and stirred under reflux for 2 h. The solvent was evaporated and the crude product was purified by column chromatography on silica gel using hexane: EtOAc (9:1) as eluent, to afford 2 in 74% yield as a colorless oil. IR n_max/cm^-1: 891, 1442, 1652, 2931, 3072. ¹H NMR (400MHz, CDCl₃) d: 1.73 (broad s, 3H), 1.75-1.83 (m, 1H), 1.91-2.00 (m, 1H), 1.96 (bs, 3H), 2.32-2.38 (m, 1H), 2.49-2.55 (m, 2H), 2.66-2.69 (m, 2H), 4.73 (m, 2H), 5.93 (broad s, 1H); ¹³C NMR (100 MHz, CDCl₃) d 20.6, 27.4, 31.2, 33.7, 40.6, 47.8, 110.0, 129.8, 148.3, 158.5, 202.5. [a]_D²⁹ + 61.3 (c = 0.02; CHCl₃).

Method (ii). The previously obtained solution of 7 (1.235 g) in MeOH (50 mL) and a saturated solution of NaOAc (20 mL) was stirred and refluxed during 2 h. The product was isolated as described above in procedure (i). The crude product was purified by column chromatography on silica gel, using hexane: EtOAc (9:1) as eluent, to afford 2 as a colorless oil in 77% yield. MS (APCI), m/z [M+H]⁺= 165. [a]_D²⁹ + 56.0 (c = 0.02; CHCl₃).

Acknowledgments

The authors wish to thank FAPESP, CAPES and CNPq for financial support and a student fellowship. The (R)-(-)-carvone (1) was generously donated by Firmenich SA, São Paulo, Brazil. The authors thank Profs. R. A. Pilli and A. Marsaioli, IQ-UNICAMP, for help with the gas chromatographic analyses on chiral columns.

Received: January 24, 2006

Published on the web: June 12, 2006

FAPESP helped in meeting the publication costs of this article.

• 1. Fraga, B. M.; Nat. Prod. Rep. 2005, 22, 465.
• 2. Brocksom, T. J.; Pesquero, E. T. C.; Lopes, F. T.; Synth. Commun 1990, 20, 1181.
• 3. De Faria, M. L.; Magalhães, R. de A.; Silva, F. C.; Matias, L. G. de O.; Ceschi, M. A.; Brocksom, U.; Brocksom, T. J.; Tetrahedron: Asymmetry 2000, 11, 4093.
• 4. Brocksom, T. J.; Brocksom, U.; de Sousa, D. P.; Frederico, D.; Tetrahedron: Asymmetry 2005, 16, 3628.
• 5. Zukerman-Schpector, J.; Caracelli, I.; Carvalho, C. C.; de Faria, M. L.; Silva, F. C.; Matias, L. G. de O.; Brocksom, T. J.; J. Braz. Chem. Soc. 2001, 12, 154.
• 6. Brocksom, T. J.; Brocksom, U.; Frederico, D.; Tetrahedron Lett. 2004, 45, 9289.
• 7. Marques, F. A.; Ferreira, J. B.; Piers, E.; J. Braz. Chem. Soc. 2000, 11, 502. We thank Prof. Marques for helpful discussions on this methodology.
• 8. Furukawa, J.; Kawabata, N.; Nishimura, J., Tetrahedron 1968, 24, 53.
• 9. Smith, R. D.; Simmons, H. E.; Org. Synth. Coll. 1973, 5, 855.
• 10. Miyano, S.; Hashimoto, H.; Chem. Commun. 1971, 1418.
• 11. Charette, A. B.; Jolicoeur, E.; Bydlinski, G. A. S.; Org. Lett. 2001, 3, 3293.
• 12. Ito, Y.; Fujii, S.; Nakatsuka, M.; Kawamoto, F.; Saegusa, T.; Org. Synth. Coll. 1998, 6, 327.

Publication Dates

• Publication in this collection
  25 Aug 2006
• Date of issue
  Aug 2006

History

• Received
  24 Jan 2006