Comparison of biochemical and immunological profile of pediatric patients with acute myeloid leukemia in relation to healthy individuals

Sanches, Fabiane L.F.Z.; Nitsch, Taís M.; Vilela, Maria Marluce S.; Sgarbieri, Valdemiro C.

doi:10.1016/j.jped.2014.12.004

Acessibilidade / Reportar erro

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

ORIGINAL ARTICLES • J. Pediatr. (Rio J.) 91 (5) • Sep-Oct 2015 • https://doi.org/10.1016/j.jped.2014.12.004 copy

Comparison of biochemical and immunological profile of pediatric patients with acute myeloid leukemia in relation to healthy individuals^☆ 3 ☆ Please cite this article as: Sanches FL, Nitsch TM, Vilela MM, Sgarbieri VC. Comparison of biochemical and immunological profile of pediatric patients with acute myeloid leukemia in relation to healthy individuals. J Pediatr (Rio J). 2015;91:478-84 ^☆☆ ☆☆ Study conducted at Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

OBJECTIVE:

To compare the biochemical and immunological profiles of pediatric patients with acute myeloid leukemia (AML) with healthy children and adolescents.

METHODS:

This was a cross-sectional study in which 21 therapy-naïve patients with AML were compared with a group of 24 healthy individuals. The following data were analyzed: serum proteins, leucocytes and subgroups, erythrocytes, hematocrit, hemoglobin, platelets, cytokines in peripheral blood mononuclear cells cultures under spontaneous and BCG- or PHA-stimulated conditions, immunoglobulin A, and erythrocytic glutathione. Statistical analysis was performed using SPSS software, considering as significant p-values < 0.05.

RESULTS:

Serum albumin levels were higher (p < 0.0001) in the control group, as well as all the parameters related to red blood cells (p < 0.0001). For leucocytes and subgroups, no statistical difference was found between the AML and the control groups. For cytokines, the concentrations were significantly higher under spontaneous and BCG-stimulated conditions for TNF-a, IL-6, IL-10, and IFN-? in the control group. Under PHA-stimulated conditions, the concentration was higher (p = 0.002) only for IL-6. No difference was found between the two groups for the other cytokines and for IgA in the saliva. Erythrocytic glutathione was higher (p < 0.0001) in AML patients.

CONCLUSIONS:

It was possible to characterize the biochemical and immunological profile of pediatric patients with AML, as well as highlight some significant differences in these parameters when comparing with healthy children and adolescents.

Keywords:
Pediatrics; Leukemia; Biochemical assessment; Immune evaluation

Serum protein		AML (n = 21)		Control (n = 24)
Albumin	Median^a	3.6		4.8
	min–max	(2.6–4.6)		(4.4–5.4)
	p ^b		<0.0001
Prealbumin	Median^a	11.75		21.45
	min–max	(3.97–24.0)		(13.1–34.6)
	p ^b		<0.0001

Parameters	AML (n = 21)	Control (n = 24)	p ^a
Leucocytes (cell × 10⁹/L)	13.1^b (1.1–494.0)	6.51^b (1.79–15.11)	0.219
Lymphocytes (cell × 10⁹/L)	2.3 (0.6–58.4)	2.8 (1.83–8.64)	0.368
Monocytes (cell × 10⁹/L)	1.3 (0.1–10.6)	0.45 (0.24–0.91)	0.308
Granulocytes (cell × 10⁹/L)	4.3 (0.1–48.6)	3.64 (1.91–5.57)	0.825
Erythrocytes (cell × 10¹²/L)	2.55 (1.78–4.36)	4.82 (4.17–5.4)	<0.0001
Hematocrit (%)	23.4 (16.6–36.6)	40.7 (34.1–47.2)	<0.0001
Hemoglobin (g/dL)	7.7 (5.6–12.5)	13.55 (11.1–15.5)	<0.0001
Plalelets (cell × 10⁹/L)	38.0 (16–220)	307.0 (162–461)	<0.0001

Cytokines	Group	Conditions
		Spontaneous	BCG	PHA
TNF-a	AML	40.491^a (0.0–418.9)	831.47^a (41.01–15,825.3)	683.17^a (38.6–1977.2)
	Control	22.42 (0.0–316.7)	4800.42 (1408.7–13,101.1)	986.61 (87.7–27,882.1)
	p ^b	0.011	<0.0001	0.052

IL-6	AML	455.641 (0.0–10,328.2)	4807.2 (41.0–31,186.7)	3010.4 (54.2–28,250)
	Control	736.17 (19.8–5044.4)	28,961.67 (25,176.7–293,344.6)	9654.65 (1904–29,051.1)
	p ^b	0.197	<0.0001	0.002

IL-10	AML	0.0 (0.0–1796.8)	124.54 (0.0–1783.05)	393.80 (63.8–1127.9)
	Control	0.0 (0.0–119.7)	615.46 (118.6–2204.9)	627.52 (61.5–1465.2)
	p ^b	0.065	0.003	0.284

IFN-?	AML	23.17 (0.0–70.6)	98.71 (0.0–1665)	150.23 (0.0–3913.8)
	Control	0.0 (0.0–48.9)	676.77 (0.0–1935.3)	87.96 (0.0–630.5)
	p ^b	0.012	0.003	0.273

Sociedade Brasileira de Pediatria Av. Carlos Gomes, 328 cj. 304, 90480-000 Porto Alegre RS Brazil, Tel.: +55 51 3328-9520 - Porto Alegre - RS - Brazil
E-mail: jped@jped.com.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] *Corresponding author. E-mail: fabianelaflor@gmail.com(F.L.F.Z. Sanches).