SUMMARY
PURPOSE:
To investigate the contribution of the deletion polymorphism and insertion (rs1799752) of the angiotensin converting enzyme (ACE) gene in the aetiology of Polycystic Ovarian Syndrome (PCOS).
METHODOLOGY:
97 women diagnosed with PCOS who received care at the Gynaecology and Obstetrics clinic of the Hospital das Clínicas of UFTM, participated in this study. The control group consisted of 94 women. All participants were submitted to the collection of 10 mL of whole blood and the genomic DNA was obtained by the saline extraction method. The genotyping of the samples was performed by means of the Polymerase Chain Reaction (PCR). The statistics analyses were performed by descriptive analysis, univariate analysis and logistic regression model. The results were presented in odds ratio (OR) and confidence interval of 95% (CI-95%), with a significance level of 5% (p≤0.05).
RESULTS:
There were no statistical differences between patients and controls for the genotypic (χ22. Andrade VH, Mata AM, Borges RS, Costa-Silva DR, Martins LM, Ferreira PM, et al. Current aspects of polycystic ovary syndrome: a literature review. Rev Assoc Med Bras. 2016;62(9):867-71. = 1.52, p = 0.47) and allelic frequencies (χ22. Andrade VH, Mata AM, Borges RS, Costa-Silva DR, Martins LM, Ferreira PM, et al. Current aspects of polycystic ovary syndrome: a literature review. Rev Assoc Med Bras. 2016;62(9):867-71. = 0.21, p = 0.76). The distribution of the genotypic frequency is not in HWE for patients (χ22. Andrade VH, Mata AM, Borges RS, Costa-Silva DR, Martins LM, Ferreira PM, et al. Current aspects of polycystic ovary syndrome: a literature review. Rev Assoc Med Bras. 2016;62(9):867-71. = 18.80, p <0.05) and for controls (χ22. Andrade VH, Mata AM, Borges RS, Costa-Silva DR, Martins LM, Ferreira PM, et al. Current aspects of polycystic ovary syndrome: a literature review. Rev Assoc Med Bras. 2016;62(9):867-71. = 6.85, p <0.05). In relation to the risk factors for the syndrome, the history of familial PCOS is more frequent between women with the syndrome.
CONCLUSION:
In the study population, there was no association between I/D polymorphism of the ACE gene and PCOS.
KEYWORDS:
Polycystic ovary syndrome; Ovarian cysts; Polymorphism, genetic; Angiotensin
M: represents the 100 pb marker of molecular weight; II: the homozygous genotype for insertion; DD: homozygous genotype for the deletion; ID: heterozygous genotype for insertion/deletion.