Evaluation of different commercial hyaluronic acids as a vehicle for injection of human adipose-derived mesenchymal stem cells

Kaleka, Camila Cohen; Zucconi, Eder; Vieira, Tierri da Silva; Secco, Mariane; Ferretti, Mário; Cohen, Moisés

doi:10.1016/j.rboe.2018.07.009

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Artigos Originais • Rev. bras. ortop. 53 (5) • Sep-Oct 2018 • https://doi.org/10.1016/j.rboe.2018.07.009 copy

Evaluation of different commercial hyaluronic acids as a vehicle for injection of human adipose-derived mesenchymal stem cells^☆ 1 Camila Cohen Kaleka and Eder Zucconi contributed equally to this study and share the authorship as the first authors of this scientific research.

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

Objective:

The main purpose of this study is to evaluate, in vitro, the cytotoxicity of different commercial brands of hyaluronic acids to be used as a vehicle for injection of human adipose-derived mesenchymal stem cells (AD-MSCs).

Methods:

AD-MSCs were divided into seven groups: one control group where AD-MSCs were cultivated with phosphate-buffered saline (PBS) and six other groups where AD-MSCs were cultivated with different commercial brands of hyaluronic acid. AD-MSC viability analysis was performed after 4, 24, and 48 h in contact with each treatment, using the trypan staining method on a Countess automated cell counter (Thermo Fisher Scientific).

Results:

The results clearly demonstrated a significant difference in cell viability when AD-MSCs were exposed to different hyaluronic acids when compared with the control group.

Conclusion:

These data suggest that hyaluronic acid can be used as a vehicle for injection of human AD-MSCs, but caution is needed to choose the best product, aiming at its future therapeutic application.

Keywords:
Cartilage diseases; Knee; Arthroscopy; Articular cartilage; Mesenchymal stem cells; Mesenchymal stem cell transplantation

Product	Molecule	Concentration	Molecular weight (MDa)	Elasticity (Pa 2.5 Hz)	Viscosity (Pa 2.5 Hz)	Source	Crosslinked molecule
Suprahyal^®	Sodium hyaluronate	1%	0.75	Viscoelasticity 1.2		Bacterial fermentation	No
Fermathron^®	Sodium hyaluronate	1%	>1	Viscoelasticity 0.84		Bacterial fermentation	No
Synvisc^®	Hylan G-F 20	0.8%	6.0	111	25	Avian	Yes
Orthovisc^®	Sodium hyaluronate	1.5%	1–2.9	60	46	Bacterial fermentation	No
Synovium^®	Sodium hyaluronate	2.5%	>2.8	160	159	Bacterial fermentation	No

Treatment	4 h		24 h		48 h
Treatment	Mean	SD	Mean	SD	Mean	SD
Fermathron^®	81.6	10.535	74.8^a a p < 0.05.	4.021	76.7	13.812
Orthovisc^®	67.4^a a p < 0.05.	10.433	66.5^a a p < 0.05.	5.792	59.6^a a p < 0.05.	13.124
Synvisc^®	81.7	7.506	67.8^a a p < 0.05.	12.631	58.2^a a p < 0.05.	14.332
Synovium^®	72.8	14.943	72.5^a a p < 0.05.	7.401	62.8	18.073
Suprahyal^®	84.1	6.809	81.2	9.915	81.6	9.717
Osteonil^®	71.4	14.3	59.1^a a p < 0.05.	16.282	71.7	11.218
PBS	90	3.464	83.5	2.933	77.4	12.347

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E-mail: rbo@sbot.org.br

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[1] *Corresponding author. E-mail: camilakaleka@gmail.com (C.C. Kaleka).

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Evaluation of different commercial hyaluronic acids as a vehicle for injection of human adipose-derived mesenchymal stem cells☆ 1 Camila Cohen Kaleka and Eder Zucconi contributed equally to this study and share the authorship as the first authors of this scientific research.

ABSTRACT

Objective:

Methods:

Results:

Conclusion:

Evaluation of different commercial hyaluronic acids as a vehicle for injection of human adipose-derived mesenchymal stem cells^☆ 1 Camila Cohen Kaleka and Eder Zucconi contributed equally to this study and share the authorship as the first authors of this scientific research.