This study reveals development of difuse peritonitis model in rats with a known bacterial concentration. We analised 150 rats, adults, mate, Wistar race with medium weight of 150 grams. Solutions of Escherichia coli and Bacteroides fragilis in diferent concentrations were percutaneous inoculated into the peritoneal cavity in the following proportion: 1 ml of each suspension to each 100 grams of the total weight of the rato the animals were allocated in five groups of thirty rats. On - group I (control) a solution of sodium cloride 0.9%. On the following groups the proportions were as follows: group II - 10(9); group III - 10(8); group 1V -10(7) and group V - 10(6). After a rat death, a necropsy was performed in order to evaluate macroscopic effects on peritoneal cavity as well collect samples of secretions for culture. The survival rats ofeach group were subdivided with no choice in two subgroups. The animals for subgroup A were sacrificed 24 hours after inoculation and the ones of subgroup B, 120 hours after inoculation. We observed that the rats of group 1 did not acquire peritonitis. On group II and III, 100% of subgroup A and 95.03% of subgroup B, developed acute peritonitis. On group II only 4.17% developed peritonitis and died 72 hours and on group V neither peritonitis or death developed. The animals which died on group II and III on an average of 96.67% showed macroscopic alterations with difused peritoneum exudate, adherences and no abscesses. The animals which developed peritonitis exhibited bilateral pleural effusion. On animals of group II and III which died, also were found the presence of Eschirichia coli and Bacteroides fragilis responsible for peritonitis and pleural effusion. This model shows that the animal which received the highest bacterial concentrations showed loss of weight, clinical alterations of sepsis, acute peritonitis with pleural effusion and premature death.
Peritonitis; Cavity abdominal punction; Bacterial inoculation