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Artigos Originais • Rev. Inst. Med. trop. S. Paulo 31 (4) • Ago 1989 • https://doi.org/10.1590/S0036-46651989000400003   copiar

Febre purpúrica brasileira: caracterização rápida das cepas invasoras de Haemophilus aegyptius

Brazilian purpuric fever: fast characterization of invasive Haemophilus aegyptius

Autoria SCIMAGO INSTITUTIONS RANKINGS

Resumos

Cepas de H. aegyptius isoladas em surtos de Febre Purpúrica Brasileira (FPB) no Brasil, foram caracterizadas pelo método de aglutinação em lâmina utilizando um anti-soro produzido com cepa de H. aegyptius isolada de cultura de sangue de paciente com FPB. Através desse método foi possível identificar cepas de H. aegyptius responsáveis por surtos de conjuntivite com características antigênicas iguais às cepas isoladas de FPB. A sensibilidade e especificidade da soroaglutinação em lâmina foi de 97,7% e 89,6% respectivamente, podendo ser utilizado como método de triagem em estudos de conjuntivites purulentas, para detectar cepas invasivas de H. aegyptius associadas a FPB, possibilitando assim a implantação de medidas que ampliem a eficiência na prevenção e na vigilância epidemiológica da doença.

Haemophilus aegyptius; Febre purpúrica brasileira; Diagnóstico laboratorial; Soroaglutinação em lâmina

Strains of H. aegyptius isolated during outbreak of Brazilian Purpuric Fever (BPF) in Brazil were characterized antigenically by slide agglutination test utilizing antiserum produced with a H. aegyptius strain isolated from blood culture from a patient with BPF. By means of this method, it were identified H. aegyptius strains responsible for outbreaks of conjunctivitis with identical antigenic characteristics to strains isolated from BPF. The sensitivity and specificity of slide seroagglutination test was 97.7% and 89.6% respectively; therefore this assay was efficient to be used as a screening method in the studies of purulent conjunctivitis for detecting high risk populations for BPF, and to implement measures that will increase the efficiency of epidemiologic surveillance.

ARTIGOS ORIGINAIS

Febre purpúrica Brasileira. Caracterização rápida das cepas invasoras de Haemophilus aegyptius

Brazilian purpuric fever. Fast characterization of invasive Haemophilus aegyptius

M.C.C. BrandileoneI; V.S.D. VieiraI; M.L.C. TondellaI; C.T. SacchiI; I.M. LandgrafI; R.C. ZanellaI; W.F. BibbII; K. IrinoI

Grupo de Estudo da Febre Purpúrica Brasileira

ISeção de Bacteriologia do Instituto Adolfo Lutz, São Paulo, Brasil

IICenters for Disease Control, Atlanta, Georgia, USA

Endereço para correspondência Endereço para correspondência: Dra. Maria Cristina de Cunto Brandileone Instituto Adolfo Lutz, Seção de Bacteriologia Av. Dr. Arnaldo, 355 CEP 01246, São Paulo, SP, Brasil

RESUMO

Cepas de H. aegyptius isoladas em surtos de Febre Purpúrica Brasileira (FPB) no Brasil, foram caracterizadas pelo método de aglutinação em lâmina utilizando um anti-soro produzido com cepa de H. aegyptius isolada de cultura de sangue de paciente com FPB. Através desse método foi possível identificar cepas de H. aegyptius responsáveis por surtos de conjuntivite com características antigênicas iguais às cepas isoladas de FPB. A sensibilidade e especificidade da soroaglutinação em lâmina foi de 97,7% e 89,6% respectivamente, podendo ser utilizado como método de triagem em estudos de conjuntivites purulentas, para detectar cepas invasivas de H. aegyptius associadas a FPB, possibilitando assim a implantação de medidas que ampliem a eficiência na prevenção e na vigilância epidemiológica da doença.

Unitermos:Haemophilus aegyptius: Febre purpúrica brasileira, Diagnóstico laboratorial; Soroaglutinação em lâmina.

SUMMARY

Strains of H. aegyptius isolated during outbreak of Brazilian Purpuric Fever (BPF) in Brazil were characterized antigenically by slide agglutination test utilizing antiserum produced with a H. aegyptius strain isolated from blood culture from a patient with BPF. By means of this method, it were identified H. aegyptius strains responsible for outbreaks of conjunctivitis with identical antigenic characteristics to strains isolated from BPF. The sensitivity and specificity of slide seroagglutination test was 97.7% and 89.6% respectively; therefore this assay was efficient to be used as a screening method in the studies of purulent conjunctivitis for detecting high risk populations for BPF, and to implement measures that will increase the efficiency of epidemiologic surveillance.

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AGRADECIMENTOS

Agradecemos o Dr. Lee Harrison, do Meningites and Special Pathogens, Division of Bacterial Diseases, Center for Disease Control, Atlanta, Estados Unidos, pelo envio das cepas do grupo 5.

REFERÊNCIAS BIBLIOGRÁFICAS

1. ALBRITTON, W.L. - Infections due to Haemophilus other than H. influenzae. Ann. Rev. Microbiol., 36:199-216, 1982.

2. ALBRITTON, W.L.; SETLOW, J.K.; THOMAS, M.; SOTTNEK, F. & STEIGERWALT, A.G. - Heterospecific transformation in the genus Haemophilus. Molec. gen. Genet., 193:358-363, 1984.

3. BRAZILIAN PURPURIC FEVER TASK FORCE - Preliminary report: epidemic fatal purpuric fever among children - Brazil. M.M.W.R., 34:217-219, 1985.

4. BRAZILIAN PURPURIC FEVER TASK FORCE - Brazilian purpuric fever: Haemophilus aegyptius bacteremia complicating purulent conjunctivitis. M.M.W.R., 35:553-554, 1986.

5. BRAZILIAN PURPURIC FEVER STUDY GROUP - Brazilian purpuric fever: epidemic purpura fulminans associated with antecedent purulent conjunctivitis. Lancet 2:757-761, 1987.

6. BRAZILIAN PURPURIC FEVER STUDY GROUP - Haemophilus aegyptius bacteraemia in Brazilian purpuric fever. Lancet, 2:761-763, 1987.

7. BRENNER, D.J.; MAYER, L.W.; CARLONE, G.M.; HARRISON, L.H.; BIBB, W.F.; BRANDILEONE, M.C.C.; SOTTNEK, F.O.; IRINO, K.; REEVES, M.W.; SWENSON, J.M.; BIRKNESS, K.A.; WEYANT, R.S.; BERKELY, S.F.; WOODS, T.C.; STEIGERWALT, A.G.; GRIMONT, P.A.D.; McKINNEY, R.M.; FLEMING, D.W.; GHEESLING, L.L.; COOKSEY, R.C.; ARKO, R.Y.; BROOME, C.V. & THE BRAZILIAN PURPURIC FEVER STUDY GROUP - Biochemical, genetic and epidemiologic characterization of Haemophilus influenzae biogroup aegyptius (Haemophilus aegyptius) strains associated with Brazilian purpuric fever. J. clin. Microbiol., 26:1524-1534, 1988.

8. DAVIS, D.J.; PITTMAN, M. & GRIFFITS, J.J. - Hemagglutination by the Koch-Weeks bacillus. J. Bact., 59:427-431, 1950.

9. FLEMING, D.W.; BERKELY, S.F.; BROOME, C.V.; BRANDILEONE, M.C.C.; WALDMAN, E.A. & THE BRAZILIAN PURPURIC FEVER STUDY GROUP - Haemophilus aegyptius conjunctivitis associated with Brazilian purpuric fever. In: ANNUAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, 88. Washington, DC, 1986. Program and abstracts. Abstr. C2.

10. HARRELL, W.K.; ASHWORTH, H.; BRITT, L.E.; GEORGE, J.R.; GRAY Jr., S.B.; GREEN, J.H.; GROSS, H. & JOHNSON, J.E. - Procedural manual of production of bacterial, fungal and parasitic reagents. Atlanta, Biological Reagents Section, Laboratory Division, Centers for Disease Control, 1970. p. 18-20.

11. IRINO, K.; LEE, I.M.L.; KAKU, M.; BRANDILEONE, M.C.C.; MELLES, C.E.A.; LEVY, C.E.; BERKLEY, S.E.; FLEMING, D.W.; SILVA, G.A. & HARRISON, L. - Febre purpúrica brasileira: resultados preliminares da investigação etiológica. Rev. Inst. Med. trop. S. Paulo, 29:174-177, 1987.

12. IRINO, K.; GRIMONT, F.; CASIN, L; GRIMONT, P.A.D. & THE BRAZILIAN PURPURIC FEVER STUDY GROUP - rDNA gene restriction patterns of Haemophilus influenzae biogroup aegyptius strains associated with the Brazilian purpuric fever. J. clin. Microbiol., 26:1535-1538, 1988.

13. KILIAN, M. & BIBERSTEIN, E.L. - Haemophilus Winslow, Broadhurst, Buchanan, Krumwiede, Robers and Smith, 1917. In: KRIEG, N.R. & HOLT, J.G., ed. - Bergey's manual of systematic bacteriology. Baltimore, Williams & Wilkins, 1984. v.1, p.558-569.

14. LEIDY, G.; HAHN, E. & ALEXANDER, H.E. - Interspecific transformation in Haemophilus: a possible index of relationship between H. influenzae and H.aegyptius. Proc. Soc. exp. Biol. (N.Y.), 102:86-88, 1959.

15. LEIDY, G.; JAFFEE, I. & ALEXANDER, H.E. - Further evidence of a high degree of genetic homology between H. influenzae and H. aegyptius. Proc. Soc. exp. Biol. (N.Y.), 118:671-679, 1964.

16. MAZLOUM, H.A.; KILIAN, M.; MOHAMED, Z. & SAID, M.D. - Differentiation of Haemophilus aegyptius and Haemophylus influenzae. Acta path, micro-bio I. scand., 90:109-112, 1982.

17. McINTYRE, P.; WHEATON, G.; ERLICH, J. & HANSMAN, D. - Brazilian purpuric fever in central Australia. Lancet, 2:112, 1987.

18. PITTMAN, M. - Variation and type specificity in bacterial species Haemophilus influenzae. J. exp. Med., 53:471-492, 1931.

19. PITTMAN, M. & DAVIS, D.J. - Identification of the Koch-Weeks Bacillus (Haemophilus aegyptius). J. Bact., 59:413-426, 1950.

20. POHL, S. - DNA relatedness among members of Actinobacillus: Haemophilus and Pasteurellae. In: KILLIAN, M.; FREDERIKSEN, W. & BIBERSTEIN, E.L., ed. - Haemophilus, Pasteurella and Actinobacillus. London, Academic Press, 1981. p.245-253.

21. PORATH, A.; WANDERMAN, K.; SIMU, A.; VIDNE, B. & ALKAN, M. - Case report: endocarditis caused by Haemophilus aegyptius. Amer. J. med. Sci., 292:110-111, 1986.

Recebido para publicação em 20/12/1988.

  • 1. ALBRITTON, W.L. - Infections due to Haemophilus other than H. influenzae. Ann. Rev. Microbiol., 36:199-216, 1982.
  • 2. ALBRITTON, W.L.; SETLOW, J.K.; THOMAS, M.; SOTTNEK, F. & STEIGERWALT, A.G. - Heterospecific transformation in the genus Haemophilus. Molec. gen. Genet., 193:358-363, 1984.
  • 3. BRAZILIAN PURPURIC FEVER TASK FORCE - Preliminary report: epidemic fatal purpuric fever among children - Brazil. M.M.W.R., 34:217-219, 1985.
  • 4. BRAZILIAN PURPURIC FEVER TASK FORCE - Brazilian purpuric fever: Haemophilus aegyptius bacteremia complicating purulent conjunctivitis. M.M.W.R., 35:553-554, 1986.
  • 5. BRAZILIAN PURPURIC FEVER STUDY GROUP -  Brazilian purpuric fever: epidemic purpura fulminans associated with antecedent purulent conjunctivitis. Lancet 2:757-761, 1987.
  • 6. BRAZILIAN PURPURIC FEVER STUDY GROUP -  Haemophilus aegyptius bacteraemia in Brazilian purpuric fever. Lancet, 2:761-763, 1987.
  • 7. BRENNER, D.J.; MAYER, L.W.; CARLONE, G.M.; HARRISON, L.H.; BIBB, W.F.; BRANDILEONE, M.C.C.; SOTTNEK, F.O.; IRINO, K.; REEVES, M.W.; SWENSON, J.M.; BIRKNESS, K.A.; WEYANT, R.S.; BERKELY, S.F.; WOODS, T.C.; STEIGERWALT, A.G.; GRIMONT, P.A.D.; McKINNEY, R.M.; FLEMING, D.W.; GHEESLING, L.L.; COOKSEY, R.C.; ARKO, R.Y.; BROOME, C.V. & THE BRAZILIAN PURPURIC FEVER STUDY GROUP - Biochemical, genetic and epidemiologic characterization of Haemophilus influenzae biogroup aegyptius (Haemophilus aegyptius) strains associated with Brazilian purpuric fever. J. clin. Microbiol., 26:1524-1534, 1988.
  • 8. DAVIS, D.J.; PITTMAN, M. & GRIFFITS, J.J. - Hemagglutination by the Koch-Weeks bacillus. J. Bact., 59:427-431, 1950.
  • 9. FLEMING, D.W.; BERKELY, S.F.; BROOME, C.V.; BRANDILEONE, M.C.C.; WALDMAN, E.A. & THE BRAZILIAN PURPURIC FEVER STUDY GROUP - Haemophilus aegyptius conjunctivitis associated with Brazilian purpuric fever. In: ANNUAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, 88. Washington, DC, 1986. Program and abstracts. Abstr. C2.
  • 10. HARRELL, W.K.; ASHWORTH, H.; BRITT, L.E.; GEORGE, J.R.; GRAY Jr., S.B.; GREEN, J.H.; GROSS, H. & JOHNSON, J.E. - Procedural manual of production of bacterial, fungal and parasitic reagents. Atlanta, Biological Reagents Section, Laboratory Division, Centers for Disease Control, 1970. p. 18-20.
  • 11. IRINO, K.; LEE, I.M.L.; KAKU, M.; BRANDILEONE, M.C.C.; MELLES, C.E.A.; LEVY, C.E.; BERKLEY, S.E.; FLEMING, D.W.; SILVA, G.A. & HARRISON, L. - Febre purpúrica brasileira: resultados preliminares da investigação etiológica. Rev. Inst. Med. trop. S. Paulo, 29:174-177, 1987.
  • 12. IRINO, K.; GRIMONT, F.; CASIN, L; GRIMONT, P.A.D. & THE BRAZILIAN PURPURIC FEVER STUDY GROUP - rDNA gene restriction patterns of Haemophilus influenzae biogroup aegyptius strains associated with the Brazilian purpuric fever. J. clin. Microbiol., 26:1535-1538, 1988.
  • 13. KILIAN, M. & BIBERSTEIN, E.L. - Haemophilus Winslow, Broadhurst, Buchanan, Krumwiede, Robers and Smith, 1917. In: KRIEG, N.R. & HOLT, J.G., ed. - Bergey's manual of systematic bacteriology. Baltimore, Williams & Wilkins, 1984. v.1, p.558-569.
  • 14. LEIDY, G.; HAHN, E. & ALEXANDER, H.E. - Interspecific transformation in Haemophilus: a possible index of relationship between H. influenzae and H.aegyptius. Proc. Soc. exp. Biol. (N.Y.), 102:86-88, 1959.
  • 15. LEIDY, G.; JAFFEE, I. & ALEXANDER, H.E. - Further evidence of a high degree of genetic homology between H. influenzae and H. aegyptius. Proc. Soc. exp. Biol. (N.Y.), 118:671-679, 1964.
  • 16. MAZLOUM, H.A.; KILIAN, M.; MOHAMED, Z. & SAID, M.D. - Differentiation of Haemophilus aegyptius and Haemophylus influenzae. Acta path, micro-bio I. scand., 90:109-112, 1982.
  • 17. McINTYRE, P.; WHEATON, G.; ERLICH, J. & HANSMAN, D. - Brazilian purpuric fever in central Australia. Lancet, 2:112, 1987.
  • 18. PITTMAN, M. - Variation and type specificity in bacterial species Haemophilus influenzae. J. exp. Med., 53:471-492, 1931.
  • 19. PITTMAN, M. & DAVIS, D.J. - Identification of the Koch-Weeks Bacillus (Haemophilus aegyptius). J. Bact., 59:413-426, 1950.
  • 20. POHL, S. - DNA relatedness among members of Actinobacillus: Haemophilus and Pasteurellae. In: KILLIAN, M.; FREDERIKSEN, W. & BIBERSTEIN, E.L., ed. - Haemophilus, Pasteurella and Actinobacillus. London, Academic Press, 1981. p.245-253.
  • 21. PORATH, A.; WANDERMAN, K.; SIMU, A.; VIDNE, B. & ALKAN, M. - Case report: endocarditis caused by Haemophilus aegyptius. Amer. J. med. Sci., 292:110-111, 1986.
  • Endereço para correspondência:
    Dra. Maria Cristina de Cunto Brandileone
    Instituto Adolfo Lutz, Seção de Bacteriologia
    Av. Dr. Arnaldo, 355
    CEP 01246, São Paulo, SP, Brasil

    • Datas de Publicação

    • Publicação nesta coleção
      14 Out 2011
    • Data do Fascículo
      Ago 1989

    Histórico

    • Recebido
      20 Dez 1988
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