Insulin reduces the requirement for serum in Plasmodium falciparum culture

Tosta, Carlos Eduardo; Sala-Neto, Felício

doi:10.1590/S0037-86821984000100009

Abstracts

Insulin added to Plasmodium falciparum cultures (0.2 IU/ml) reduced the requirement for human serum from ten to five percent. This represents an obvious advantage by its serum-sparing effect and by reducing the chances of using contaminated serum in cultures. The growth-promoting ability of insulin was observed eitherin culture- adapted P. falciparum or in newly-isolated samples.

Plasmodium falciparum; Malaria; Insulin; Culture medium

A adição de insulina (0,2) UI/ml) à cultura de Plasmodium falciparum reduziu a necessidade de soro humano de dez para cinco por cento. Isto representa uma óbvia vantagem não só pela economia de soro como pela diminuição do risco de se utilizar amostras de soro contaminadas nas culturas. Esta capacidade da insulina de promover a multiplicação de plasmódio foi observada tanto em relação à P. falciparum adaptado à cultura por 12 meses como em amostras recém-isoladas do parasito.

Plasmodium falciparum; Malária; Insulina; Meio de cultura

FOREWORD

Insulin reduces the requirement for serum in Plasmodium falciparum culture

Carlos Eduardo Tosta; Felício Sala-Neto

^{Adress to correspondence} Adress to correspondence: Dr. Carlos Eduardo Tosta Laboratório de Imunologia Celular Departamento de Medicina Complementar Universidade de Brasília 70910 Brasília, Brasil.

ABSTRACT

Insulin added to Plasmodium falciparum cultures (0.2 IU/ml) reduced the requirement for human serum from ten to five percent. This represents an obvious advantage by its serum-sparing effect and by reducing the chances of using contaminated serum in cultures. The growth-promoting ability of insulin was observed eitherin culture- adapted P. falciparum or in newly-isolated samples.

Keywords:Plasmodium falciparum. Malaria. Insulin. Culture medium.

RESUMO

A adição de insulina (0,2) UI/ml) à cultura de Plasmodium falciparum reduziu a necessidade de soro humano de dez para cinco por cento. Isto representa uma óbvia vantagem não só pela economia de soro como pela diminuição do risco de se utilizar amostras de soro contaminadas nas culturas. Esta capacidade da insulina de promover a multiplicação de plasmódio foi observada tanto em relação à P. falciparum adaptado à cultura por 12 meses como em amostras recém-isoladas do parasito.

Palavras-chave:Plasmodium falciparum. Malária. Insulina. Meio de cultura.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

Recebido para publicação em 31/12/1983.

Work from the Laboratório de Imunologia Celular, MDC, Universidade de Brasília, supported by grants from CNPq and UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Medicine.

1. Brown RL, Griffith RL, Neubauer RH, Rabin H. Development of a serum-free medium which supports the long-term growth of human and non-human primate lymphoid cells. Journal of Cellular Physiology 115:191-198, 1983.
2. Chapronière-Rickenberg DM. Zinc levels in zinc- stabilized insulin are inhibitory to the growth of cells in vitro. In Vitro 19: 373-375, 1983.
3. Divo AA, Jensen JB. Studies on serum requirements for the cultivítion of Plasmodium falciparum I. Animal sera. Bulletin of the World Health Organization 60:565-569, 1982.
4. Jensen JB. Some aspects of serum requirements for continuous cultivation of Plasmodium falciparum Bulletin of the World Health Organization 57(Suppl 1): 27-31, 1979.
5. Mather JP, Sato GH. The growth of mouse melanoma cells in hormone-supplemented, serum-free medium. Experimental Cell Research 120:191-200, 1979.
6. Taub M, Livingston D. The development of serum-free hormone-supplemented media for primary kidney cultures and their use in examining renal functions. Annals of the New York Academy of Sciences 372:406- 421, 1981.
7. Trager W. Cultivation of erythrocytic stages of malaria. In: The in vitro cultivation of pathogens of tropical diseases. Tropical Diseases Research Series n.° 3, Schwabe, Basel, PP 3-13, 1980.
8. Trager W, Jensen JB. Human malaria parasites in continuous culture. Science 193: 673-675, 1976.

Adress to correspondence:

Dr. Carlos Eduardo Tosta

Laboratório de Imunologia Celular

Departamento de Medicina Complementar

Universidade de Brasília

70910

Brasília, Brasil.

Publication Dates

Publication in this collection
10 June 2013
Date of issue
Mar 1984

History

Received
31 Dec 1983
Accepted
31 Dec 1983

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. Brown RL, Griffith RL, Neubauer RH, Rabin H. Development of a serum-free medium which supports the long-term growth of human and non-human primate lymphoid cells. Journal of Cellular Physiology 115:191-198, 1983.

[2] 2. Chapronière-Rickenberg DM. Zinc levels in zinc- stabilized insulin are inhibitory to the growth of cells in vitro. In Vitro 19: 373-375, 1983.

[3] 3. Divo AA, Jensen JB. Studies on serum requirements for the cultivítion of Plasmodium falciparum I. Animal sera. Bulletin of the World Health Organization 60:565-569, 1982.

[4] 4. Jensen JB. Some aspects of serum requirements for continuous cultivation of Plasmodium falciparum Bulletin of the World Health Organization 57(Suppl 1): 27-31, 1979.

[5] 5. Mather JP, Sato GH. The growth of mouse melanoma cells in hormone-supplemented, serum-free medium. Experimental Cell Research 120:191-200, 1979.

[6] 6. Taub M, Livingston D. The development of serum-free hormone-supplemented media for primary kidney cultures and their use in examining renal functions. Annals of the New York Academy of Sciences 372:406- 421, 1981.

[7] 7. Trager W. Cultivation of erythrocytic stages of malaria. In: The in vitro cultivation of pathogens of tropical diseases. Tropical Diseases Research Series n.° 3, Schwabe, Basel, PP 3-13, 1980.

[8] 8. Trager W, Jensen JB. Human malaria parasites in continuous culture. Science 193: 673-675, 1976.