Physiological limitations in two sugarcane varieties under water suppression and after recovering

Medeiros, David Barbosa; Silva, Elizamar Ciríaco da; Nogueira, Rejane Jurema Mansur Custódio; Teixeira, Marcelo Menossi; Buckeridge, Marcos Silveira

Abstract

Increasing water scarcity and depleted water productivity in irrigated soils are inducing farmers to adopt improved varieties, such as those with high-capacity tolerance. The use of tolerant varieties of sugarcane might substantially avoid the decline of productivity under water deficit. This research aimed to evaluate the harmful effects of drought on the physiology of two sugarcane varieties (RB867515 and RB962962) during the initial development. Young plants were subjected to irrigation suspension until total stomata closure, and then rewatered. Significant reduction on stomatal conductance, transpiration, and net photosynthesis were observed. RB867515 showed a faster stomatal closure while RB962962 slowed the effects of drought on the gas exchanges parameters with a faster recovering after rewatering. Accumulation of carbohydrates, amino acids, proline, and protein in the leaves and roots of the stressed plants occurred in both varieties, substantially linked to reduction of the leaf water potential. Due to the severity of stress, this accumulation was not enough to maintain the cell turgor pressure, so relative water content was diminished. Water stress affected the contents of chlorophyll (a, b, and total) in both varieties, but not the levels of carotenoids. There was a significant reduction in dry matter under stress. In conclusion, RB962962 variety endured stressed conditions more than RB867515, since it slowed down the damaging effects of drought on the gas exchanges. In addition, RB962962 presented a faster recovery than RB867515, a feature that qualifies it as a variety capable of enduring short periods of drought without major losses in the initial stage of its development.

drought; photosynthesis; Saccharum spp; gas exchange

Ashraf M, Foolad MR (2007) Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany 59:206-216.
Barrs HD, Weatherley PE (1962) A re-examination of the relative turgidity technique for estimating water deficit in leaves. Australian Journal of Biological Science 15:413-428.
Bates LS (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205-207.
Begcy K, Mariano ED, Gentile A, Lembke CG, Zingaretti SM, Souza GM, Menossi M (2012) A novel stress-induced sugarcane gene confers tolerance to drought, salt and oxidative stress in transgenic tobacco plants. PLoS One 7:e44697.
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248-250.
Buckeridge MS, Souza AP, Arundale RA, Kristina J. Anderson-Teixeira KJ, Delucia E (2012) Ethanol from sugarcane in Brazil: a 'midway' strategy for increasing ethanol production while maximizing environmental benefits. Global Change Biology Bioenergy 4:119-126.
Cha-um S, Kirdmanee C (2009) Effect of osmotic stress on proline accumulation, photosynthetic abilities and growth of sugarcane plantlets (Saccharum officinarum L.). Pakistan Journal of Botany 40:2541-2552.
Cha-um S, Wangmoon S, Mongkolsiriwatana C, Ashraf M, Kirdmanee C (2012) Evaluating sugarcane (Saccharum sp.) cultivars for water deficit tolerance using some key physiological markers. Plant Biotechnology 29:431-439.
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350-356.
Edwards D, Batley E (2010) Plant genome sequencing: applications for crop improvement. Plant Biotechnology Journal 8:2-9.
Egert M, Tevini M (2002) Influence of drought on some physiological parameters symptomatic for oxidative stress in leaves of chives (Allium schoenoprasum). Environmental and Experimental Botany 48:43-49.
Farooq M, Wahid A, Kobayashi N, Fujita D, Basra SMA (2009) Plant drought stress: effects, mechanisms and management. Agronomy for Sustainable Development 29:185-212.
Ferreira TH, Gentile A, Vilela RD, Costa GGL, Dias LI, Endres L, Menossi M (2012) microRNAs associated with drought response in the bioenergy crop sugarcane (Saccharum spp.). PLoS One 7:e46703.
Gentile A, Ferreira TH, Mattos RS, Dias LI, Hoshino AA, Carneiro MS, Souza GM, Calsa Jr. T, Nogueira RM, Endres L, Menossi M (2013) Effects of drought on the microtranscriptome of field-grown sugarcane plants. Planta 237:783-798.
Gonçalves ER, Ferreira VM, Silva JV, Endres L, Barbosa TP, Duarte WG (2010) Gas exchange and chlorophyll a fluorescence of sugarcane varieties submitted to water stress . Revista Brasileira de Engenharia Agrícola e Ambiental 14:378-386.
Graça JP, Godrigues FA, Farias JRB, Oliveira MCN, Hoffmann-Campo CB, Zingaretti SM (2010) Physiological parameters in sugarcane cultivars submitted to water deficit. Brazilian Journal of Plant Physiology 22:189-197.
Guimarães ER, Mutton MA, Mutton MJR, Ferro MIT, Ravaneli GC, Silva JA (2008) Free proline accumulation in sugarcane under water restriction and spittlebug infestation. Scientia Agricola 65:628-633.
Hasegawa PM, Bressan RA, Zhu Jian-Kang, Bohnert HJ (2000) Plant cellular and molecular responses to high salinity. Annual Review of Plant Physiology and Plant Molecular Biology 51:463-499.
Hemaprabha G, Nagarajan R, Alarmelu S (2004) Response of sugarcane genotypes to water deficit stress. Sugar Tech 6:165-168.
Hessini K, Martínez JP, Gandoura M, Albouchib A, Soltania A, Abdellya C (2009) Effect of water stress on growth, osmotic adjustment, cell wall elasticity and water-use efficiency in Spartina alterniflora Environmental and Experimental Botany 67:312-319.
Inman-Bamber NG, Smith DM (2005) Water relations in sugarcane and response to water deficits. Field Crops Research 92:185-202.
Inman-Bamber, NG (2004) Sugarcane water stress criteria for irrigation and drying off. Field Crops Research 89:107-122.
Jangpromma N, Songsri P, Thammasirirak S, Jaisil P (2010) Rapid assessment of chlorophyll content in sugarcane using a SPAD chlorophyll meter across different water stress conditions. Asian Journal of Plant Sciences 9:368-374.
Jaleel CA, Manivannan P, Wahid A, Farooq M, Somasundaram R, Panneerselvam R (2009) Drought stress in plants: a review on morphological characteristics and pigments composition. International Journal of Agriculture and Biology 11:100-105.
Kavi Kishor PB, Sangam S, Amrutha RN, Sri Laxmi P, Naidu KR, Rao KRSS, Sreenath Rao Reddy KJ, Theriappan P, Sreenivasulu N (2005) Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress tolerance. Current Science 88:424-438.
Lawlor DW (2013) Genetic engineering to improve plant performance under drought: physiological evaluation of achievements, limitations, and possibilities. Journal of Experimental Botany 64:83-108.
Larcher, W (2006) Ecofisiologia vegetal. Translation: Prado CHBA. 1st ed. São Carlos: Rima.
Liberato MAR, Gonçalves JFC, Chevreuil LR, Nina Junior AR, Fernandes AV, Santos Junior UM. 2006. Leaf water potential, gas exchange and chlorophyll a fluorescence in acariquara seedlings (Minquartia guianensis Aubl.) under water stress and recovery. Brazilian Journal of Plant Physiology 18:315-323.
Lichtenthaler HK, Buschmann C (2001) Chlorophylls and carotenoids – Measurement and characterisation by UV-VIS. In: Current protocols in food analytical chemistry Madison: John Wiley & Sons. F4.3.1–F4.3.8.
Medeiros DB, Silva EC, Santos HRB, Pacheco CM, Musser RS, Nogueira RJMC (2012) Physiological and biochemical response to drought stress in the Barbados cherry. Brazilian Journal of Plant Physiology 24:181-192.
Nogueira RJMC, Moraes JAPV, Burity HA (2000) Modifications in vapor diffusion resistence of leaves and water relations in barbados cherry plants under water stress. Pesquisa Agropecuária Brasileira 35:1331-1342.
Nogueira RJMC, Moraes JAPV, Burity HÁ, Bezerra Neto E (2001) Modifications in vapor diffusion resistance of leaves and water relations in Barbados cherry plants under water stress. Revista Brasileira de Fisiologia Vegetal 13:75-87.
Pimentel C (1999) Gas exchange and chlorophyll a fluorescence of sugarcane varieties submitted to water stress. Pesquisa Agropecuária Brasileira 34:2021-2027.
Queiroz RJB, Santos DMM, Carlin SD, Marin A, Banzatto DA, Cazetta JO (2008) Effects of osmoprotectors on sugarcane plants growing under different water deficit levels. Científica 36:107-115.
Scholander PF, Hammel HT, Hemingsen EA, Bradstreet ED (1964) Hydrostatic pressure and osmotic potentials in leaves of mangroves and some other plants. Proceeding of the National Academy of Science 52:119-125.
Silva EC, Albuquerque MB, Azevedo Neto AD, Silva Junior CD (2013) Drought and its consequences to plants from individual to ecosystem. In: Sener Akinci, editor. Responses of organisms to water stress Croatia: InTech. pp.17-47.
Silva EC, Nogueira RJMC, Vale FHA, Melo NF, Araujo FP (2009) Water relations and organic solutes production in four umbu tree (Spondias tuberosa) genotypes under intermittent drought. Brazilian Journal of Plant Physiology 21:43-53.
Silva EC, Silva MFA, Nogueira RJMC, Albuquerque MB (2010) Growth evaluation and water relations of Erythrina velutina seedlings in response to drought stress. Brazilian Journal of Plant Physiology 22:225-233.
Silva MA, Jifon JL, Silva JAG, Sharma V (2007) Use of physiological parameters as fast tools to screen for drought tolerance in sugarcane. Brazilian Journal of Plant Physiology 19:193-201.
Simões Neto DE, Melo LJOT, Chaves A, Lima ROR (2005) Levantamento de novas variedades RB de cana-de-açúcar. Boletim técnico 1. Recife: UFRPE, Imprensa Universitária. 28p.
Sircelj H, Tausz M, Grill D, Batic F (2005) Biochemical responses in leaves of two apple tree cultivars subjected to progressing drought. Journal of Plant Physiology 162:1308-1318.
Souza CC, Oliveira FA, Silva IF, Amorim Neto MS (2000) Evaluation of methods of available water determination and irrigation management in 'terra roxa' under cotton crop. Revista. Brasileira de Engenharia Agrícola e Ambiental 4:338-342.
Souza RP, Machado EC, Silva JAB, Lagôa AMMA, Silveira JAG (2004) Photosynthetic gas exchange in cowpea (Vigna unguiculata) during water stress and recovery. Environmental and Experimental Botany 51:45-56.
Yemm EW, Cocking EC (1955) The determination of aminoacids by ninhydrin. Analyst 80:209-213.

Publication Dates

Publication in this collection
02 Dec 2013
Date of issue
2013

History

Received
17 Jan 2013
Accepted
11 Oct 2013

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] Ashraf M, Foolad MR (2007) Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany 59:206-216.

[2] Barrs HD, Weatherley PE (1962) A re-examination of the relative turgidity technique for estimating water deficit in leaves. Australian Journal of Biological Science 15:413-428.

[3] Bates LS (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205-207.

[4] Begcy K, Mariano ED, Gentile A, Lembke CG, Zingaretti SM, Souza GM, Menossi M (2012) A novel stress-induced sugarcane gene confers tolerance to drought, salt and oxidative stress in transgenic tobacco plants. PLoS One 7:e44697.

[5] Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248-250.

[6] Buckeridge MS, Souza AP, Arundale RA, Kristina J. Anderson-Teixeira KJ, Delucia E (2012) Ethanol from sugarcane in Brazil: a 'midway' strategy for increasing ethanol production while maximizing environmental benefits. Global Change Biology Bioenergy 4:119-126.

[7] Cha-um S, Kirdmanee C (2009) Effect of osmotic stress on proline accumulation, photosynthetic abilities and growth of sugarcane plantlets (Saccharum officinarum L.). Pakistan Journal of Botany 40:2541-2552.

[8] Cha-um S, Wangmoon S, Mongkolsiriwatana C, Ashraf M, Kirdmanee C (2012) Evaluating sugarcane (Saccharum sp.) cultivars for water deficit tolerance using some key physiological markers. Plant Biotechnology 29:431-439.

[9] Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350-356.

[10] Edwards D, Batley E (2010) Plant genome sequencing: applications for crop improvement. Plant Biotechnology Journal 8:2-9.

[11] Egert M, Tevini M (2002) Influence of drought on some physiological parameters symptomatic for oxidative stress in leaves of chives (Allium schoenoprasum). Environmental and Experimental Botany 48:43-49.

[12] Farooq M, Wahid A, Kobayashi N, Fujita D, Basra SMA (2009) Plant drought stress: effects, mechanisms and management. Agronomy for Sustainable Development 29:185-212.

[13] Ferreira TH, Gentile A, Vilela RD, Costa GGL, Dias LI, Endres L, Menossi M (2012) microRNAs associated with drought response in the bioenergy crop sugarcane (Saccharum spp.). PLoS One 7:e46703.

[14] Gentile A, Ferreira TH, Mattos RS, Dias LI, Hoshino AA, Carneiro MS, Souza GM, Calsa Jr. T, Nogueira RM, Endres L, Menossi M (2013) Effects of drought on the microtranscriptome of field-grown sugarcane plants. Planta 237:783-798.

[15] Gonçalves ER, Ferreira VM, Silva JV, Endres L, Barbosa TP, Duarte WG (2010) Gas exchange and chlorophyll a fluorescence of sugarcane varieties submitted to water stress . Revista Brasileira de Engenharia Agrícola e Ambiental 14:378-386.

[16] Graça JP, Godrigues FA, Farias JRB, Oliveira MCN, Hoffmann-Campo CB, Zingaretti SM (2010) Physiological parameters in sugarcane cultivars submitted to water deficit. Brazilian Journal of Plant Physiology 22:189-197.

[17] Guimarães ER, Mutton MA, Mutton MJR, Ferro MIT, Ravaneli GC, Silva JA (2008) Free proline accumulation in sugarcane under water restriction and spittlebug infestation. Scientia Agricola 65:628-633.

[18] Hasegawa PM, Bressan RA, Zhu Jian-Kang, Bohnert HJ (2000) Plant cellular and molecular responses to high salinity. Annual Review of Plant Physiology and Plant Molecular Biology 51:463-499.

[19] Hemaprabha G, Nagarajan R, Alarmelu S (2004) Response of sugarcane genotypes to water deficit stress. Sugar Tech 6:165-168.

[20] Hessini K, Martínez JP, Gandoura M, Albouchib A, Soltania A, Abdellya C (2009) Effect of water stress on growth, osmotic adjustment, cell wall elasticity and water-use efficiency in Spartina alterniflora Environmental and Experimental Botany 67:312-319.

[21] Inman-Bamber NG, Smith DM (2005) Water relations in sugarcane and response to water deficits. Field Crops Research 92:185-202.

[22] Inman-Bamber, NG (2004) Sugarcane water stress criteria for irrigation and drying off. Field Crops Research 89:107-122.

[23] Jangpromma N, Songsri P, Thammasirirak S, Jaisil P (2010) Rapid assessment of chlorophyll content in sugarcane using a SPAD chlorophyll meter across different water stress conditions. Asian Journal of Plant Sciences 9:368-374.

[24] Jaleel CA, Manivannan P, Wahid A, Farooq M, Somasundaram R, Panneerselvam R (2009) Drought stress in plants: a review on morphological characteristics and pigments composition. International Journal of Agriculture and Biology 11:100-105.

[25] Kavi Kishor PB, Sangam S, Amrutha RN, Sri Laxmi P, Naidu KR, Rao KRSS, Sreenath Rao Reddy KJ, Theriappan P, Sreenivasulu N (2005) Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress tolerance. Current Science 88:424-438.

[26] Lawlor DW (2013) Genetic engineering to improve plant performance under drought: physiological evaluation of achievements, limitations, and possibilities. Journal of Experimental Botany 64:83-108.

[27] Larcher, W (2006) Ecofisiologia vegetal. Translation: Prado CHBA. 1st ed. São Carlos: Rima.

[28] Liberato MAR, Gonçalves JFC, Chevreuil LR, Nina Junior AR, Fernandes AV, Santos Junior UM. 2006. Leaf water potential, gas exchange and chlorophyll a fluorescence in acariquara seedlings (Minquartia guianensis Aubl.) under water stress and recovery. Brazilian Journal of Plant Physiology 18:315-323.

[29] Lichtenthaler HK, Buschmann C (2001) Chlorophylls and carotenoids – Measurement and characterisation by UV-VIS. In: Current protocols in food analytical chemistry Madison: John Wiley & Sons. F4.3.1–F4.3.8.

[30] Medeiros DB, Silva EC, Santos HRB, Pacheco CM, Musser RS, Nogueira RJMC (2012) Physiological and biochemical response to drought stress in the Barbados cherry. Brazilian Journal of Plant Physiology 24:181-192.

[31] Nogueira RJMC, Moraes JAPV, Burity HA (2000) Modifications in vapor diffusion resistence of leaves and water relations in barbados cherry plants under water stress. Pesquisa Agropecuária Brasileira 35:1331-1342.

[32] Nogueira RJMC, Moraes JAPV, Burity HÁ, Bezerra Neto E (2001) Modifications in vapor diffusion resistance of leaves and water relations in Barbados cherry plants under water stress. Revista Brasileira de Fisiologia Vegetal 13:75-87.

[33] Pimentel C (1999) Gas exchange and chlorophyll a fluorescence of sugarcane varieties submitted to water stress. Pesquisa Agropecuária Brasileira 34:2021-2027.

[34] Queiroz RJB, Santos DMM, Carlin SD, Marin A, Banzatto DA, Cazetta JO (2008) Effects of osmoprotectors on sugarcane plants growing under different water deficit levels. Científica 36:107-115.

[35] Scholander PF, Hammel HT, Hemingsen EA, Bradstreet ED (1964) Hydrostatic pressure and osmotic potentials in leaves of mangroves and some other plants. Proceeding of the National Academy of Science 52:119-125.

[36] Silva EC, Albuquerque MB, Azevedo Neto AD, Silva Junior CD (2013) Drought and its consequences to plants from individual to ecosystem. In: Sener Akinci, editor. Responses of organisms to water stress Croatia: InTech. pp.17-47.

[37] Silva EC, Nogueira RJMC, Vale FHA, Melo NF, Araujo FP (2009) Water relations and organic solutes production in four umbu tree (Spondias tuberosa) genotypes under intermittent drought. Brazilian Journal of Plant Physiology 21:43-53.

[38] Silva EC, Silva MFA, Nogueira RJMC, Albuquerque MB (2010) Growth evaluation and water relations of Erythrina velutina seedlings in response to drought stress. Brazilian Journal of Plant Physiology 22:225-233.

[39] Silva MA, Jifon JL, Silva JAG, Sharma V (2007) Use of physiological parameters as fast tools to screen for drought tolerance in sugarcane. Brazilian Journal of Plant Physiology 19:193-201.

[40] Simões Neto DE, Melo LJOT, Chaves A, Lima ROR (2005) Levantamento de novas variedades RB de cana-de-açúcar. Boletim técnico 1. Recife: UFRPE, Imprensa Universitária. 28p.

[41] Sircelj H, Tausz M, Grill D, Batic F (2005) Biochemical responses in leaves of two apple tree cultivars subjected to progressing drought. Journal of Plant Physiology 162:1308-1318.

[42] Souza CC, Oliveira FA, Silva IF, Amorim Neto MS (2000) Evaluation of methods of available water determination and irrigation management in 'terra roxa' under cotton crop. Revista. Brasileira de Engenharia Agrícola e Ambiental 4:338-342.

[43] Souza RP, Machado EC, Silva JAB, Lagôa AMMA, Silveira JAG (2004) Photosynthetic gas exchange in cowpea (Vigna unguiculata) during water stress and recovery. Environmental and Experimental Botany 51:45-56.

[44] Yemm EW, Cocking EC (1955) The determination of aminoacids by ninhydrin. Analyst 80:209-213.

Brasil

Brasil

Physiological limitations in two sugarcane varieties under water suppression and after recovering

Abstract

Publication Dates

History