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Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946

Abstract

ANDRADE, Ana Paula Franco de et al. Immunoperoxidase for the detection for the detection of antibodies in cerebrospinal fluid in neurocysticercosis: use of Cysticercus cellulosae and Cysticercus longicollis particles fixed on microscopy slides. Rev. Inst. Med. trop. S. Paulo [online]. 1996, vol.38, n.4, pp.259-264. ISSN 1678-9946.  http://dx.doi.org/10.1590/S0036-46651996000400004.

The ORF strain of Cysticercus longicollis represents an important model for the study of heterologous antigens in the immunodiagnosis of neurocysticercosis (NC). The immunoperoxidase (IP) technique was standardized using a particulate antigen suspension of Cysticercus longicollis (Cl) and Cysticercus cellulosae (Cc). Cerebrospinal fluid (CSF) samples were incubated on the antigen fixed to microscopy slides; the conjugate employed was anti-IgG-peroxidase and the enzymatic reaction was started by covering the slides with chromogen solution (diaminobenzidine/H2O2). After washing with distilled water, the slide was stained with 2% malachite green in water. Of the CSF samples from 21 patients with NC, 19 (90.5%) were positive, whereas the 8 CSF samples from the control group (100%) were negative. The results of the IP-Cl test applied to 127 CSF samples from patients with suspected NC showed 28.3% reactivity as opposed to 29.1 % for the IP-Cc test. The agreement index for the IP test (Cl x Cc) was 94.2%, with no significant difference between the two antigens.

Keywords : Immunoperoxidase; Neurocysticercosis; Cysticercus cellulosae; Cysticercus longicollis; Cerebrospinal fluid.

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