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Fitopatologia Brasileira

Print version ISSN 0100-4158On-line version ISSN 1678-4677


MEINHARDT, LYNDEL W.; WULFF, NELSON A.; BELLATO, CLÁUDIA M.  and  TSAI, SIU M.. Telomere and microsatellite primers reveal diversity among Sclerotinia sclerotiorum isolates from Brazil. Fitopatol. bras. [online]. 2002, vol.27, n.2, pp.211-215. ISSN 0100-4158.

Sclerotinia sclerotiorum, the causal agent of white mold, is a problem of winter bean (Phaseolus vulgaris) production in Brazil under center-pivot irrigation. Isolates of S. sclerotiorum were obtained from a center-pivot-irrigated field near Guaíra-SP, Brazil. Mycelial compatibility group (MCG) studies revealed the presence of only two MCG. PCR/RFLP analysis of the ITS1-5.8S-ITS2 ribosomal subunit regions of these field isolates of S. sclerotiorum failed to show any genetic differences between these two MCGs. DNA amplification with a chromosomal telomere sequence-based primer and one microsatellite primer revealed genetic polymorphisms among isolates within the same MCG. Isolates taken from beans and two other crops from another region of Brazil showed the same two MCG and had identical banding patterns for the telomere and microsatellite primers. These findings support the use of telomere sequence-based primers for revealing genotypic differences among S. sclerotiorum isolates.

Keywords : genetic diversity; mycelial compatibility group; Phaseolus vulgaris.

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