Abstract

GOUVEA, Ana Cristina Miranda Senna et al. Anthocyanins standards (cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside) isolation from freeze-dried açaí (Euterpe oleraceae Mart.) by HPLC. Ciênc. Tecnol. Aliment. [online]. 2012, vol.32, n.1, pp. 43-46.  Epub Jan 24, 2012. ISSN 0101-2061.  http://dx.doi.org/10.1590/S0101-20612012005000001.

Availability of analytical standards is a critical aspect in developing methods for quantitative analysis of anthocyanins. The anthocyanins cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside were isolated from samples of freeze-dried açaí (Euterpe oleraceae Mart.), which is a round and purple well-known palm fruit in Brazil, and then used as standards. The isolation of the anthocyanins was performed by High Performance Liquid Chromatography (HPLC), using an adapted six-channel selection valve. The identification of anthocyanin pigments in açaí was based on mass spectrometric data for molecular ions and MS-MS product ions and on previous published data. After the collection procedure, standards with a high purity grade were obtained and an external standard curve of each anthocyanin was plotted.

Keywords : anthocyanins; standard; isolation; açaí; Euterpe oleraceae Mart.; HPLC.