|
|
Primer 1, |
AGCAGGCAAGGG TTCTATTACTAAAT |
100 |
88 |
50 |
100 |
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Primer 2, |
GAAGCAACAGCA GATTCTCAACA |
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AAGACCCTAAAGACTTT |
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Hirsch et al. 22 Hirsch HH, Knowles W, Dickenmann M, Passweg J, Klimkait T, Mihatsch MJ, et al. Prospective study of polyomavirus type BK replication and nephropathy in renal-transplant recipients. N Engl J Med 2002;347:488-96. PMID: 12181403 DOI: http://dx.doi.org/10.1056/NEJMoa020439
http://dx.doi.org/10.1056/NEJMoa020439...
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NI |
Probe |
CCCTCTGATCTACA CCAGTTT labeled with 6-carboxyfluorescein at the 5` end and |
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6-carboxytetramethylrhodamine at the |
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3` end |
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BKpangF |
ATGTGACCA ACACAGC |
60 |
76 |
65 |
72 |
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BKpangR |
CTG TGCCATCAAACACC |
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Pang et al.1111 Pang XL, Doucette K, LeBlanc B, Cockfield SM, Preiksaitis JK. Monitoring of polyomavirus BK virus viruria and viremia in renal allograft recipients by use of a quantitative real-time PCR assay: one-year prospective study. J Clin Microbiol 2007;45:3568-73. PMID: 17855578 DOI: http://dx.doi.org/10.1128/JCM.00655-07
http://dx.doi.org/10.1128/JCM.00655-07...
|
VP1 gene |
BKpangP1 |
AGGAGAACCCAGA GAGTGGA-fluorescein |
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BKpangP2 |
LC-Red 640-GGCAGCCTATGT ATGGTATGGAA-phosphate |
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(5`-AGG TAG AAG AGG TTA GGG TGT |
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TTG ATG GCA CAG-3`) dual-labeled at |
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Thamboo et al. 2828 Thamboo TP, Jeffery KJ, Friend PJ, Turner GD, Roberts IS. Urine cytology screening for polyoma virus infection following renal transplantation: the Oxford experience. J Clin Pathol 2007;60:927-30. DOI: http://dx.doi.org/10.1136/jcp.2006.042507
http://dx.doi.org/10.1136/jcp.2006.04250...
|
VP1 gene |
NI |
the 5` end with 6-carboxyfluorsecein (FAM) and the 3` end with |
67 |
33 |
20 |
80 |
|
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6-carboxytetramethylrhodamine |
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(TAMRA) |
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Primer PoL1s, |
CACTTTTGGGGGACCTAGT |
100 |
96 |
50 |
100 |
Viscount et al.88 Viscount HB, Eid AJ, Espy MJ, Griffin MD, Thomsen KM, Harmsen WS, Razonable RR, et al. Polyomavirus polymerase chain reaction as a surrogate marker of polyomavirus-associated nephropathy. Transplantation 2007;84:340-5. PMID: 17700158 DOI: http://dx.doi.org/10.1097/01.tp.0000275205.41078.51
http://dx.doi.org/10.1097/01.tp.00002752...
|
VP2 gene |
Primer PoL2as, Probe 1, |
CTCTACAGTAGCA AGGGATGC TCTGAGGCTGCTGCT |
|
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PoLP1, |
GCCACAGGATTTT-fluorescein |
|
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Probe 2, |
LC-Red 640-AGTAG CTGAAATTGCTG |
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PoLP2, |
CTGGAGAGGCTGCT-phosphate |
|
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|
Primer PoL1s, |
CACTTTTGGGGGACCTAGT |
100 |
91 |
15 |
100 |
Almerás et al. 2424 Alméras C, Foulongne V, Garrigue V, Szwarc I, Vetromile F, Segondy M, et al. Does reduction in immunosuppression in viremic patients prevent BK virus nephropathy in de novo renal transplant recipients? A prospective study. Transplantation 2008;85:1099-104. PMID: 18431228 DOI: http://dx.doi.org/10.1097/TP.0b013e31816a33d4
http://dx.doi.org/10.1097/TP.0b013e31816...
|
VP2 gene |
Primer PoL2as, Probe 1, PoLP1, |
CTCTACAGTAGCAAGGGATGC TCTGAGGCTGC TGCTGCCA CAGGATTTT-fluorescein |
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Probe 2, PoLP2, |
LC-Red 640-AGTAGCTG AAATTGCTGC TGGAGAGGCTGCT-phosphate |
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Babel et al. 1010 Babel N, Fendt J, Karaivanov S, Bold G, Arnold S, Sefrin A, et al. Sustained BK viruria as an early marker for the development of BKV-associated nephropathy: analysis of 4128 urine and serum samples. Transplantation 2009;88:89-95. PMID: 19584686 DOI: http://dx.doi.org/10.1097/TP.0b013e3181aa8f62
http://dx.doi.org/10.1097/TP.0b013e3181a...
|
VP1 gene |
NI |
NI |
100 |
96 |
43 |
100 |
Girmanova et al. 2929 Girmanova E, Brabcova I, Bandur S, Hribova P, Skibova J, Viklicky O. A prospective longitudinal study of BK virus infection in 120 Czech renal transplant recipients. J Med Virol 2011;83:1395-400. PMID: 21618550 DOI: http://dx.doi.org/10.1002/jmv.22106
http://dx.doi.org/10.1002/jmv.22106...
|
Gene that encode large T Ag |
Commercial kit |
BKV Q-PCR Detection Alert Kit (Chemagen) |
100 |
68 |
7 |
100 |
Pollara et al. 1616 Pollara CP, Corbellini S, Chiappini S, Sandrini S, De Tomasi D, Bonfanti C, et al. Quantitative viral load measurement for BKV infection in renal transplant recipients as a predictive tool for BKVAN. New Microbiol 2011;34:165-71.
|
Gene that encode large T Ag |
Commercial kit |
BKV Q.Alert Kit (Nanogen Advanced Diagnostics, Italy) |
95 |
100 |
NI |
NI |
Saundh et al. 99 Saundh BK, Baker R, Harris M, Welberry Smith MP, Cherukuri A, Hale A. Early BK polyomavirus (BKV) reactivation in donor kidney is a risk factor for development of BKV-associated nephropathy. J Infect Dis 2013;207:137-41. DOI: http://dx.doi.org/10.1093/infdis/jis642
http://dx.doi.org/10.1093/infdis/jis642...
|
Gene that encode large T Ag |
BKV Forward BKV Reverse BKV Probe |
TGA CTA AGA AAC TGG TGT AGA TCA YTCC TT TAAT GA AAA ATG GGA FAM AGT GTT GAG AAT CTG CTG TTG CTT C BHQ-1 |
100 |
91 |
17 |
100 |
Knight et al. 2121 Knight RJ, Gaber LW, Patel SJ, DeVos JM, Moore LW, Gaber AO. Screening for BK viremia reduces but does not eliminate the risk of BK nephropathy: a single-center retrospective analysis. Transplantation 2013;95:949-54. DOI: http://dx.doi.org/10.1097/TP.0b013e31828423cd
http://dx.doi.org/10.1097/TP.0b013e31828...
|
NI |
NI |
NI |
100 |
87 |
26 |
100 |
|
|
Primer 1, |
AGCAGGCAAGGGTTCTATTACTAAAT |
100 |
57 |
41 |
100 |
|
|
Primer 2, |
GAAGCAACAGCAGATTCTCAACA |
|
|
|
|
Menter et al. 2323 Menter T, Mayr M, Schaub S, Mihatsch MJ, Hirsch HH, Hopfer H. Pathology of resolving polyomavirus-associated nephropathy. Am J Transplant 2013;13:1474-83. DOI: http://dx.doi.org/10.1111/ajt.12218
http://dx.doi.org/10.1111/ajt.12218...
|
NI |
Probe |
AAGACCCTAAAGACTTTCCCTCTGAT CTACACCAGTTT labeled with 6-carboxyfluorescein at the 5` end and 6-carboxytetramethylrhodamine at the 3` end |
|
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