SciELO - Scientific Electronic Library Online

vol.23 número3LC, LC-MS/MS studies for the identification and characterization of degradation products of hydrochlorothiazide and establishment of mechanistic approach towards degradationShort-term toxicity test: monitoring Klebsiella oxytoca bacterium respiration using a flow injection analysis/conductometric system índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados




Links relacionados


Journal of the Brazilian Chemical Society

versão impressa ISSN 0103-5053


PELUSA, Héctor F. et al. Quantification of autoantibodies to annexin V in plasma by an "in house" sandwich ELISA. J. Braz. Chem. Soc. [online]. 2012, vol.23, n.3, pp.453-460. ISSN 0103-5053.

An "in house" sandwich ELISA for the quantification of plasma anti-annexin V antibodies was developed and validated. The validation parameters studied were: (i) precision, expressed either as the intra- or the inter-assay coefficient of variation (CV), (ii) exactitude, expressed as the percentage deviation between the obtained value and the real value, (iii) limit of detection (LOD), evaluated from the reagents blank and (iv) robustness, obtained by deliberately introducing slight variations in different parameters. Also, a comparison between the "in house" technique and a commercial method was performed. The research revealed that both CV were < 20%, exactitude was within the 100 ± 20% range, limit of detection was below 1 U mL-1 and that slight variations in the technique did not produce any significant variations in the results. Comparison with the commercial method showed an acceptable correlation. It was concluded that the method developed here satisfactorily accomplishes the parameters of standardization and validation for an immunoassay.

Palavras-chave : ELISA; annexin V; antibodies; anti-phospholipid syndrome; foetal losses.

        · resumo em Português     · texto em Inglês     · Inglês ( pdf epdf )


Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons