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Brazilian Archives of Biology and Technology
On-line version ISSN 1678-4324
FILGUEIRAS, Cristina Tostes and VANETTI, Maria Cristina Dantas. Effect of eugenol on growth and listeriolysin o production by Listeria monocytogenes. Braz. arch. biol. technol. [online]. 2006, vol.49, n.3, pp. 405-409. ISSN 1678-4324. http://dx.doi.org/10.1590/S1516-89132006000400008.
The inhibitory effect of eugenol, a naturally occurring compound mainly present in the essential oil fraction of cloves, was studied on the growth and listeriolysin O (LLO) production by Listeria monocytogenes. Potassium efflux from cells promoted by eugenol was also determined after 24 h incubation in phosphate buffered saline. Eugenol promoted a delay on the growth of L. monocytogenes at concentrations of 100, 300 and 500 µg mL-1and above 800 µg mL-1 the effect was bactericidal. Production of LLO by L. monocytogenes in the presence of eugenol was reduced 80-100%. An accumulation of external K+ was observed above 300 µg mL-1 of eugenol which indicated that the cell membrane was affected. The results showed the effectiveness of eugenol in controlling growth and LLO production of L. monocytogenes cells.
Keywords : Eugenol; Listeria monocytogenes; antilisteric; LLO.