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Brazilian Archives of Biology and Technology
On-line version ISSN 1678-4324
ROMAO, Silvia et al. Blood parameter analysis and morphological alterations as biomarkers on the health of Hoplias malabaricus and Geophagus brasiliensis. Braz. arch. biol. technol. [online]. 2006, vol.49, n.3, pp. 441-448. ISSN 1678-4324. http://dx.doi.org/10.1590/S1516-89132006000400012.
This study aimed to assess the influence of the environment on fish health. Samples of Hoplias malabaricus and Geophagus brasiliensis, were collected from three different environments: area I was urban and areas II and III were rural. Analyses of red blood cell count, microhematocrit, hemoglobin concentration, white blood cell count and differential white cell count in blood smear were carried out. Mean corpuscular volume and mean corpuscular hemoglobin concentration were calculated. To analyze morphological alterations, gills, liver, kidney and gonads were submitted to routine histological processing. Individuals collected from area III had slightly lower blood indices than collected from area I . Severe kidney changes, degeneration of and crystallization within kidney tubules were observed. In area I, crystallization was observed in 92% of the specimens of G. brasiliensis. These results suggested that such alterations were related with poor water circulation in the place.
Keywords : Fish; bioindicators; biomarkers; morphological alterations; hematology.