SUMMARY
The aim of this work was to evaluate the antimicrobial activity of plant extracts Ocotea silvestris Vattimo-Gil and Ocotea diospyrifolia (Meisn.) Mez against 10 serotypes Salmonella enterica of poultry origin of the highest incidence in the western region of Paraná, as well as to perform the phytochemical screening of the compounds of the two species. After obtaining the aqueous extract and the organic extracts (ethyl alcohol, ethyl acetate and hexane) was determined Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) by technique microdilution broth. Phytochemical prospecting performed according to qualitative methodology of precipitation and colorimetry. The extracts presented inhibitory and bactericidal activity in different concentrations (200 to 25 mg.mL-1) against the serotypes of Salmonella, varying according to the solvent extractor. The ethyl acetate extract presented the best results, followed by the alcoholic, aqueous and hexane extract. It is noteworthy that studies of antimicrobial activity with vegetable extract within the genus Ocotea are scarce, however, the Lauraceae family presents significant biological activities, mainly related to its essential oils. Phytochemical prospecting detected the presence of steroids and alkaloids in both Ocotea species, which may justify the antimicrobial potential found for Salmonella serotypes. It is concluded that these plants represent an alternative for the control of Salmonella in the poultry sector, reflecting a new perspective for studies with natural products.
Keywords:
bactericidal; bacteriostatic; Lauraceae; microdilution