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Brazilian Journal of Pharmaceutical Sciences

versão On-line ISSN 2175-9790


ANTUNES, Marina Venzon; WAGNER, Sandrine Comparsi; CAMARGO, Joíza Lins  e  LINDEN, Rafael. Standardization of method for determining glycosylated hemoglobin (Hb A1c) by cation exchange high performance liquid chromatography. Braz. J. Pharm. Sci. [online]. 2009, vol.45, n.4, pp.650-657. ISSN 2175-9790.

Hemoblobin A1c is the most important parameter for the monitoring of metabolic control of patients with diabetes mellitus. The purpose of this study was to adapt the Mono S method to a conventional HPLC system, allowing highly selective HbA1c determination without the acquisition of kits or the use of dedicated systems The results obtained were compared to the Tinaquant® immune turbidimetric method and the Bio-Rad Variant® chromatographic method. The developed method presented intra-study precision (C.V. %) of 1.39-3.65 and inter-study precision (C.V. %) of 2.80-3.02%. The determination coefficients among methods were: HPLC Mono S x Tinaquant®: r2: 0.9856 (n=60) and HPLC Mono S x HPLC Bio-Rad Variant®: r2: 0.9806 (n=16). A conversion equation between HPLC Mono S and Bio-Rad Variant® was calculated allowing yielding comparable and interchangeable values. The HPLC Mono-S is a precise, low-cost method which yields similar values to the Bio-Rad Variant® method on conventional HPLC equipment.

Palavras-chave : High performance liquid chromatography; Mono S [method]; Hemoglobin [A1c]; Hemoglobin [variants].

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