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Brazilian Journal of Pharmaceutical Sciences

On-line version ISSN 2175-9790


QUEIROZ, Kelly Marques et al. Comparison of microbiological assay and HPLC-UV for determination of fluconazole in capsules. Braz. J. Pharm. Sci. [online]. 2009, vol.45, n.4, pp.693-700. ISSN 2175-9790.

The development of a specific agar diffusion bioassay for the quantitative determination of fluconazole formulated in capsules was carried out using a strain of Candida albicans ATCC 18804 as the test organism. A prospective validation of the method showed adequate linearity (r2=0.9995), precision (R.S.D. = 4.0% for intra-day and 4.5% for inter-day precision) and accuracy (mean recovery = 102.9%). High performance liquid chromatography was chosen as a comparison method for the fluconazole determination. The contents of fluconazole determined by both methods, for four capsule samples, showed a strong correlation, confirmed by Pearson's correlation coefficient value (r = 0.9884). The bioassay is a suitable method for both research and pharmaceutical industry laboratories.

Keywords : Fluconazole [quantitative determination]; Microbiological assay [agar diffusion]; High performance liquid chromatography.

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