American trypanosomiasis (Chagas' disease) in conventional and germfree rats and mice

Silva, Marcelo E.; Evangelista, Elizio A.; Nicoli, Jacques R.; Bambirra, Eduardo A.; Vieira, Enio C.

doi:10.1590/S0036-46651987000500004

Abstracts

Germfree (GF) and conventional (CV) CFW (LOB) mice and Wistar and Sprague-Dawley rats were infected with Trypanosoma cruzi. The disease was more severe in the GF than in the CV animals as revealed by: (1) an earlier and more intense parasitemia; (2) a more precocious mortality; (3) a twice enlarged spleen: (4) a more intense cell and tissue parasitism; (5) visceral signs of cardiac failure.

Chagas' disease; Trypanosomiasis; Germfree rats; Germfree mice

Camundongos CFW (LOB) e ratos Wistar e Sprague-Dawley isentos de germes (GF) e convencionais (CV) foram infectados com Trypanosoma cruzi. A doença foi mais grave nos animais GF do que nos CV, o que foi demonstrado por: (1) uma parasitemia mais precoce e mais intensa: (2) uma mortalidade mais precoce: (3) baço duas vezes maior; (4) um parasitismo celular e tissular mais intenso; (5) sinais viscerais de insuficiência cardíaca.

ORIGINAL ARTICLES

American trypanosomiasis (Chagas' disease) in conventional and germfree rats and mice (¹ (1 ) This work was extracted from the M. S. thesis of MES. )

Tripanosomíase americana (doença de Chagas) em ratos e camundongos convencionais e isentos de germes

Marcelo E. Silva^I; Elizio A. Evangelista^II; Jacques R. Nicoli^II; Eduardo A. Bambirra^III; Enio C. VieiraIII

^IDepartamento de Nutrição, Escola de Farmácia, Universidade Federal de Ouro Preto, Ouro Preto, MG., Brasil

^IIDepartamento de Bioquímica Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG) Belo Horizonte, MG., Brasil

^IIIDepartamento de Anatomia Patológica, Faculdade de Medicina, UFMG, Belo Horizonte, MG., Brasil

SUMMARY

Germfree (GF) and conventional (CV) CFW (LOB) mice and Wistar and Sprague-Dawley rats were infected with Trypanosoma cruzi. The disease was more severe in the GF than in the CV animals as revealed by: (1) an earlier and more intense parasitemia; (2) a more precocious mortality; (3) a twice enlarged spleen: (4) a more intense cell and tissue parasitism; (5) visceral signs of cardiac failure.

Key words: Chagas' disease; Trypanosomiasis; Germfree rats; Germfree mice.

RESUMO

Camundongos CFW (LOB) e ratos Wistar e Sprague-Dawley isentos de germes (GF) e convencionais (CV) foram infectados com Trypanosoma cruzi. A doença foi mais grave nos animais GF do que nos CV, o que foi demonstrado por: (1) uma parasitemia mais precoce e mais intensa: (2) uma mortalidade mais precoce: (3) baço duas vezes maior; (4) um parasitismo celular e tissular mais intenso; (5) sinais viscerais de insuficiência cardíaca.

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ACKNOWLEDGEMENTS

This work was supported by Financiadora de Estudos e Projetos (FINEP) and by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). The technical help of Ronilda M. de Paula and Carlos Gomes Silva is acknowledged.

Recebido para publicação em 16/1/87.

1. BRENER, Z. - Therapeutic activity and criterion of cure on mice experimentally infected with Trypanosoma cruzi Rev. Inst. Med. trop. S. Paulo, 4: 389-396, 1962.
2. CULBERTSON, J. T. & KESSLER, W. R. - Age resistance of mice to Trypanosoma cruzi J. Parasit., 28: 155-158, 1942.
3. FINERTY, J. F.; TOBIE, J. E. & EVANS, C. B. - Antibody and immunoglobulin synthesis in germfree and conventional mice infected with Plasmodium berghei Amer. J. trop. Med. Hyg., 21: 499-505, 1972.
4. GOODMAN, M. G.; FIDLER, J. M. & WEIGLE, W. O. - Nonspecific activation of murine lymphocytes. III. Cells responding rriitogenically to 2 mercaptoethanol are typi cal unstimulated lymphocytes. J. Immunol., 121: 1899-1904, 1978.
5. GORDON, H. A. & PESTI, L. - The gnotobiotic animal as a tool in the study of host microbial relationship. Bact. Rev., 35: 390-429, 1971.
6. HYDE, C. L.; FINERTY, J. F. & EVANS, C. B. - Antibody and immunoglobulin synthesis in germfree and conventional mice infected with Eperythrozoon coccoides Amer. J. trop. Med. Hyg., 21: 506-511, 1972.
7. KIYONO, H.; McGHEE, J. R. & MICHALEK, S. M. - LPS regulation of the immune response: comparison of response to LPS in germfree, Escherichia coli-monoassociated and conventional mice. J. Immunol., 124: 36-41, 1980.
8. KOLODNY, M. H. - Studies on age resistance against trypanosome infection. I. The resistance of rats of different ages to infection with T. cruzi Amer. J. Hyg., 29: 13-24, 1939.
9. KOLODNY, M. H. - The effect of environmental temperature upon experimental trypanosomiasis (T. cruzi) of rats. Amer. J. Hyg., 32: 21-23, 1940.
10. OLSON, G. B. & WOSTMANN, B. S. - Lymphocytopoiesis, plasmocytopoiesis and cellular proliferation in nonantigenically stimulated germfree mice. J. Immunol., 97: 267-274, 1966a.
11. OLSON, G. B. & WOSTMANN, B. S. - Cellular and humoral immune response of germfree mice stimulated with 7s HGGor Salmonella typhimurium J. Immunol., 97: 275-286, 1966b.
12. PLEASANTS, J. R. - Gnoto biotics. In: MELBY JR., E. C & ALTMAN, N. H. ed. - Handbook of laboratory animal science. Cleveland, CRC Press, 1974. p. 119-174.
13. POLLARD, M. - Viral status of germfree mice. Natl. Cancer Inst. Monograph., 20: 167-172, 1965
14. ROGERS, T. J. & BALISH, E. - Effect of the systemic candidiasis on blastogenesis of lymphocytes from germfree and conventional rats. Infect. Immun., 20: 142-150, 1978.
15. SELL, S. & FAHEY, J. L. - Relationship between γ-globulin metabolism and low serum γ-globulin in germfree mice. J. Immunol., 93: 81-87, 1964.
16. SZERI, I., ANDERLIK, P.; BANOS, Z. & RADNAI, B. - Decreased cellular response in germ free animals. Acta Microbiol. Acad. Sci. hung., 23: 231-234, 1976.
17. TREXLER, P. C. - The use of plastics in the design of isolator systems. Ann. N. Y. Acad. Sci., 78: 29-36, 1959.
18. VIEIRA, L. Q.; MORAES E SANTOS, T. & VIEIRA, E C. - Alterations in cell number, size and collagen content in livers of conventional and germfree mice bearing Schistosoma mansoni In. WOSTMANN, B. S., ed. - Germfree research: Microflora. Control and its application to the biomedical sciences. New York, Alan R. Liss, 1985. p. 235-238.
19. WAGNER, M. - Determination of germfree status. Ann. N. Y. Acad. Sci., 78: 89-101, 1959.
20. WOODWARD, B. - A stereological ultrastructural study of peritoneal macrophages from germfree and conventionally reared mice. Cell Tiss. Res., 192: 157-166. 1978.

(1

) This work was extracted from the M. S. thesis of MES.

Publication Dates

Publication in this collection
17 Feb 2011
Date of issue
Oct 1987

History

Received
16 Jan 1987

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. BRENER, Z. - Therapeutic activity and criterion of cure on mice experimentally infected with Trypanosoma cruzi Rev. Inst. Med. trop. S. Paulo, 4: 389-396, 1962.

[2] 2. CULBERTSON, J. T. & KESSLER, W. R. - Age resistance of mice to Trypanosoma cruzi J. Parasit., 28: 155-158, 1942.

[3] 3. FINERTY, J. F.; TOBIE, J. E. & EVANS, C. B. - Antibody and immunoglobulin synthesis in germfree and conventional mice infected with Plasmodium berghei Amer. J. trop. Med. Hyg., 21: 499-505, 1972.

[4] 4. GOODMAN, M. G.; FIDLER, J. M. & WEIGLE, W. O. - Nonspecific activation of murine lymphocytes. III. Cells responding rriitogenically to 2 mercaptoethanol are typi cal unstimulated lymphocytes. J. Immunol., 121: 1899-1904, 1978.

[5] 5. GORDON, H. A. & PESTI, L. - The gnotobiotic animal as a tool in the study of host microbial relationship. Bact. Rev., 35: 390-429, 1971.

[6] 6. HYDE, C. L.; FINERTY, J. F. & EVANS, C. B. - Antibody and immunoglobulin synthesis in germfree and conventional mice infected with Eperythrozoon coccoides Amer. J. trop. Med. Hyg., 21: 506-511, 1972.

[7] 7. KIYONO, H.; McGHEE, J. R. & MICHALEK, S. M. - LPS regulation of the immune response: comparison of response to LPS in germfree, Escherichia coli-monoassociated and conventional mice. J. Immunol., 124: 36-41, 1980.

[8] 8. KOLODNY, M. H. - Studies on age resistance against trypanosome infection. I. The resistance of rats of different ages to infection with T. cruzi Amer. J. Hyg., 29: 13-24, 1939.

[9] 9. KOLODNY, M. H. - The effect of environmental temperature upon experimental trypanosomiasis (T. cruzi) of rats. Amer. J. Hyg., 32: 21-23, 1940.

[10] 10. OLSON, G. B. & WOSTMANN, B. S. - Lymphocytopoiesis, plasmocytopoiesis and cellular proliferation in nonantigenically stimulated germfree mice. J. Immunol., 97: 267-274, 1966a.

[11] 11. OLSON, G. B. & WOSTMANN, B. S. - Cellular and humoral immune response of germfree mice stimulated with 7s HGGor Salmonella typhimurium J. Immunol., 97: 275-286, 1966b.

[12] 12. PLEASANTS, J. R. - Gnoto biotics. In: MELBY JR., E. C & ALTMAN, N. H. ed. - Handbook of laboratory animal science. Cleveland, CRC Press, 1974. p. 119-174.

[13] 13. POLLARD, M. - Viral status of germfree mice. Natl. Cancer Inst. Monograph., 20: 167-172, 1965

[14] 14. ROGERS, T. J. & BALISH, E. - Effect of the systemic candidiasis on blastogenesis of lymphocytes from germfree and conventional rats. Infect. Immun., 20: 142-150, 1978.

[15] 15. SELL, S. & FAHEY, J. L. - Relationship between γ-globulin metabolism and low serum γ-globulin in germfree mice. J. Immunol., 93: 81-87, 1964.

[16] 16. SZERI, I., ANDERLIK, P.; BANOS, Z. & RADNAI, B. - Decreased cellular response in germ free animals. Acta Microbiol. Acad. Sci. hung., 23: 231-234, 1976.

[17] 17. TREXLER, P. C. - The use of plastics in the design of isolator systems. Ann. N. Y. Acad. Sci., 78: 29-36, 1959.

[18] 18. VIEIRA, L. Q.; MORAES E SANTOS, T. & VIEIRA, E C. - Alterations in cell number, size and collagen content in livers of conventional and germfree mice bearing Schistosoma mansoni In. WOSTMANN, B. S., ed. - Germfree research: Microflora. Control and its application to the biomedical sciences. New York, Alan R. Liss, 1985. p. 235-238.

[19] 19. WAGNER, M. - Determination of germfree status. Ann. N. Y. Acad. Sci., 78: 89-101, 1959.

[20] 20. WOODWARD, B. - A stereological ultrastructural study of peritoneal macrophages from germfree and conventionally reared mice. Cell Tiss. Res., 192: 157-166. 1978.