Print version ISSN 0036-4665
Rev. Inst. Med. trop. S. Paulo vol.54 no.1 São Paulo Jan./Feb. 2012
Diagnosticando co-infecção leishmaniose visceral e HIV/AIDS: uma série de casos em Pernambuco, Brasil
Aracele Tenório de Almeida e CavalcantiI; Zulma MedeirosI,II; Fábio LopesII; Luiz Dias de AndradeII; Valéria de Melo FerreiraIII; Vera MagalhãesIV; Demócrito de Barros Miranda-FilhoI
IUniversidade de Pernambuco, Brazil
IIFundação Oswaldo Cruz - FIOCRUZ (Centro de Pesquisa Aggeu Magalhães-Laboratório de Doenças Transmissíveis)
IIILaboratório Central de Saúde Pública do estado de Pernambuco (Laboratório Dr. Milton Bezerra Sobral)
IVUniversidade Federal de Pernambuco (Hospital das Clínicas), Brazil
HIV/AIDS-associated visceral leishmaniasis may display the characteristics of an aggressive disease or without specific symptoms at all, thus making diagnosis difficult. The present study describes the results of diagnostic tests applied to a series of suspected VL cases in HIV-infected/AIDS patients admitted in referral hospitals in Pernambuco, Brazil. From a total of 14 eligible patients with cytopenias and/or fever of an unknown etiology, and indication of bone marrow aspirate, 10 patients were selected for inclusion in the study. Diagnosis was confirmed by the following examinations: Leishmania detection in bone marrow aspirate, direct agglutination test, indirect immunofluorescence, rK39 dipstick test, polymerase chain reaction and latex agglutination test. Five out of the ten patients were diagnosed with co-infection. A positive direct agglutination test was recorded for all five co-infected patients, the Leishmania detection and latex agglutination tests were positive in four patients, the rK39 dipstick test in three, the indirect immunofluorescence in two and a positive polymerase chain reaction was recorded for one patient. This series of cases was the first to be conducted in Brazil using this set of tests in order to detect co-infection. However, no consensus has thus far been reached regarding the most appropriate examination for the screening and monitoring of this group of patients.
Key words: Visceral Leishmaniasis; HIV/AIDS; HIV/VL co-infection; Diagnostic tests.
A associação da leishmaniose visceral com o HIV/AIDS pode se manifestar com características de doença agressiva ou sem sintomas específicos, dificultando o diagnóstico. Este artigo descreve o resultado de testes diagnósticos aplicados a uma série de casos suspeitos de leishmaniose visceral em pacientes com HIV/AIDS internados em hospitais de referência de Pernambuco, Brasil. De 14 pacientes elegíveis com citopenias e/ou febre de etiologia indefinida, com indicações de mielograma, foram incluídos dez. Para o diagnóstico, foram empregados os seguintes exames: pesquisa de Leishmania em aspirado de medula óssea, DAT, IFI, rK39, PCR e a KAtex. Cinco dos dez pacientes foram diagnosticados com co-infecção. Entre os cinco co-infectados, o DAT foi positivo em todos, a pesquisa de Leishmania foi positiva em quatro, assim como o KAtex, o rK39 em três, a IFI em dois e a PCR positiva em apenas um. Esta série de casos foi a primeira realizada no Brasil utilizando esse conjunto de exames para detecção de co-infecção, no entanto, ainda não há consenso quanto aos exames mais adequados a serem aplicados para screening e acompanhamento desse grupo de pacientes.
In HIV-infected patients, visceral leishmaniasis (VL) accelerates the onset of AIDS and the disease can progress rapidly into severe forms22,24. HIV-VL co-infection may appear clinically in its classical form or with very aggressive characteristics, sometimes non-specific and difficult to diagnose29.
The association between the diseases is recent and has an increasing number of cases in Brazil and worldwide - especially in the Mediterranean region of Europe15,18. In Brazil, a phenomenon of overlapping infections characterized by the ruralization of AIDS and urbanization of VL has been observed, which indicates the emergence of parasitic disease as an important infection opportunistic to HIVpatients11. However, there are very few reports from Brazil concerning co-infection6,10,17,25.
The present study describes the results of diagnostic tests applied to a series of suspected VL cases in HIV-infected/AIDS patients.
PATIENTS AND METHODS
HIV-infected/AIDS patients were included in the study after they were admitted to four public hospitals in Pernambuco, Brazil, between September 2008 and January 2010. The study included 18-year-old patients, who presented cytopenia (leucocyte count < 3,000/mm3, hemoglobin concentration < 10 g/dL, platelet count <120,000/mm3) in at least two cell lines and/or fever (> 38 °C) for more than eight days, with no defined etiology and indication of bone marrow aspirate from the doctor's assistant. The study was approved by the Research Ethics Committee at the Aggeu Magalhães Research Center - Fundação Oswaldo Cruz (CAAE 0121.0.095.000-08).
HIV diagnosis was confirmed by Western blot or indirect immunofluorescence7 and VL by identifying amastigotes in bone marrow aspirates and/or positive results in KAtex12, with good therapeutic responses.
Evidence for a favorable response to therapy (clinical remission) was considered when the patient was afebrile and when physical signs as well as laboratory abnormalities significantly improved after a complete course of treatment1.
The direct agglutination test (DAT) was used to detect anti-Leishmania antibodies. A result was considered positive when ≥ 1/6,40021. The reaction to indirect immunofluorescence (IFA) was considered positive when fluorescence was observed in a dilution of ≥ 1/8018 and the rK39 dipstick test was considered positive when two lines were visualized on the strip9.
For the polymerase chain reaction in peripheral blood (PCR - illustra TM tissue & cells genomicPrep Mini Spin KIT), RV1 and RV2 primers were used15. DNA bands were separated by electrophoresis and visualized under a UV light, and photographed with the Kodak Gel Logic 100 Imaging System, using Kodak molecular imaging software 4.0.0. The latex agglutination test (Katex - Kalon Biological Ltd's, UK) was categorized as: positive +++, and ++, and +, and negative5.
Of the 14 eligible patients, two died before undergoing the bone marrow aspirate and two were excluded due to the existence of other diagnoses (tuberculosis and lymphoma). Nine patients were previously diagnosed as having AIDS and other case was diagnosed with AIDS during a screening of VL.
The mean age was 36.1 years (27-44 years/±5.8). Five patients had attended school for 11 or more years and five owned a dog. All ten patients presented splenomegaly, weight loss and asthenia and one reported no fever.
Nine patients were male and came from the metropolitan region of Recife, of which eight were from the urban area and one from the rural area (Table 1).
Five co-infected cases were identified from amongst ten who were included in the study. Among the five cases of co-infection, there were two cases of VL (first episode) and three cases of relapse. Four patients tested positive for Leishmania in the bone marrow aspirate. A positive PCR was recorded for only one of these patients and nine patients obtained a positive DAT. Patient number 6 began a therapeutic test with amphotericin B prior to completing the examination for Leishmania in bone marrow aspirate, and tested negative, although the KAtex (++) and DAT (1/12,800) were positive. A clinical improvement was observed with the therapeutic test: hematological counts were normalized and splenomegaly and fever reduced. Of the ten HIV-infected patients, nine (five with VL and four without VL) had a positive serologic test (DAT).
The mean age of the five co-infected patients was 37.2 (31-44 years/± 6.0). Four were male, from the metropolitan region of Recife, and the only female patient was from the rural area. The female patient of this series was an agricultural worker, living in the agricultural zone of Pernambuco, and reported cases of VL at home (partner) and around the local neighborhood. Two of the male patients maintained sexual relations with other men and the female was in a stable relationship with her HIV-infected partner. None of the patients reported intravenous drug use. Two of these patients had attended school for more than 11 years and also owned dogs.
A positive DAT was recorded in all co-infected patients (1/12,800 - 1/1,638,400). Four patients presented a positive KAtex test (++/+++), and one did not perform the examination. Three patients recorded a positive rK39 dipstick test; two a positive IFA (1/80) and one patient presented a positive PCR (Table 1).
The mean CD4 count was 208 cells/mm3 (56-399/+153). Four patients were taking antiretrovirals (ART) and one was diagnosed with VL concomitantly with AIDS. The five patients diagnosed as co-infected were treated with amphotericin B. Three of the five cases diagnosed in the study reported previous episodes of co-infection, two of them relapsed in less than one year after treatment and did not receive prophylaxis, the other relapsed two years after discontinuation of prophylaxis with amphotericin B.
Five co-infected cases were identified from amongst ten who presented clinical indications of bone marrow aspirate, signs and symptoms compatible with classical forms of VL. The symptoms presented by the co-infected patients in this series may occur in other situations associated with AIDS, which can hinder or delay a clinical suspicion of VL10,22.
All patients had splenomegaly. Febrile splenomegaly and cytopenia in HIV-infected patients may also be observed in tuberculosis26, histoplasmosis8 and lymphoma23. Cytopenias are frequent during the course of HIV infection and may result from several mechanisms24. In addition, is important to be alert for possible situations of co-infection where manifestations of VL are atypically presented28.
In this series, one of the five co-infected patients was diagnosed with VL concomitantly with AIDS. Visceral leishmaniasis may be the first infection related in 13% up to 47% of HIV-infected patients22,24. It is important to make an early diagnosis and monitor the development of leishmaniasis in HIV-infected patients, since these cases are less responsive to treatment and have higher fatality rates30.
All patients with previous episodes of VL had taken ART. In 2008, Ter Horst, et al.32, reported a reduction in the occurrence of new episodes with the use of ART. Mira et al. 19, however, concluded that if the patients had a high viral load and a low immune response, ART proved ineffective in preventing new episodes of the disease.
Microscopic observation of bone marrow aspirate is very specific (100%), but sensitivity has been reported between 67% and 94%9,24. Several reasons have been cited for the limited sensitivity: a low number of Leishmania-infected cells as a consequence of pancytopenia; a hemodiluted sample due to inadequate collection or a low parasite load when the patient has already started therapy20,30. Patient six tested negative for Leishmania, but presented positive results with DAT and KAtex. This case was considered co-infection and showed a good therapeutic response.
Urinary antigen detection (KAtex test) was able to positively diagnose all cases of infection even after initiating amphotericin B. However, it has been reported that antigenuria becomes negative after successful chemotherapy, suggesting that KAtex may be a useful tool for monitoring the efficacy of the treatment2. In another two studies of co-infected patients in Spain, KAtex had a sensitivity of 85.7% and 100%, respectively, when the parasite load was high27,34. Attar et al.,5 reported the potential use of this test to diagnose active infection, although the need still exists for additional studies in order to provide a better definition of the role of KAtex, both in investigating the infection and during post-therapy follow-up. The present report of the use of KAtex in co-infected patients is the first publication on the Brazilian population. Whether the test is applicable to detecting asymptomatic or subclinical populations has still not been sufficiently clarified5,13,27.
The rK39 dipstick test confirmed the myelogram in all cases of recurrence, and no false-positive results were observed. In a study by Sundar et al. 31, in India, the rK39 dipstick format has proved 100% sensitive and 98% specific when compared to splenic aspirates. Another validation study, conducted in a tertiary care hospital in southeastern Nepal, compared DAT with the rK39 dipstick test on 184 patients with a clinical suspicion of VL, and found a sensitivity of 97% and a specificity of 71% for the rK39 dipstick test9. The ease of use of the rK39 dipstick test and its low cost enable early diagnosis of VL in decentralized settings thereby improves access to early life-saving treatment33.
ASSIS et al.4, reporting in VL sensitivities and specificities of the DAT were 90% and 96%, respectively. The authors concluded that DAT constituted a useful test and can replace IFAT as the routine diagnostic test used by the Brazilian Leishmaniasis Control Program. There is no consensus regarding the ideal cutoff point for co-infected patients. A positive DAT was recorded for all co-infected patients, but four out of the five patients not considered co-infected with VL also tested positive. The lowest titer amongst co-infected patients was 1/12,800 (patient 7) and the highest was 1/1,638,400 (patient 10). Two of the patients considered without VL had borderline titers (1/6,400) and the other two obtained high titers for DAT (1/25,600 and 1/> 51,200). One of them was diagnosed with a liver abscess and the other a pulmonary abscess. DAT, despite being one of the cheapest, simplest and most sensitive for diagnosing VL14, does not differentiate between past and recent infections35. The DAT test was not a diagnost criterion for co-infection study. This finding may be explained by a high prevalence of VL in the area. Due to the low sensitivity of bone marrow aspirate (67-94%),9,24 it is possible that patients with positive serology and negative aspirate actually had VL. The use of KAtex can, however, increase the sensitivity for the diagnosis of active disease12,13.
Regarding serologic findings in this study, the functional impairment of cell-mediated immunity due to HIV infection may result in the absence of an antibody response to Leishmania infection, even when that is parasitemia. This impairment of the immune system may explain the higher frequency of false-negative antibody tests results in co-infected patients29. Chappuis et al.,9 suggest that patients who test positive with rK39 dipstick test or present elevated DAT titers, with a history of leishmaniasis, should be submitted to a Leishmania test by bone marrow or spleen aspirate in order to investigate co-infection.
No significant anti-Leishmania antibody titers were detected by immunofluorescence, and only two of the five cases tested positive. The results of the present study are similar to studies that have described low titers for IFA in diagnosing co-infections24,29. The three cases with a new episode of co-infection presented positive results for rK39 dipstick test as well as DAT.
The PCR used was positive in only one of the five patients. The technique and the primers used in the PCR of this series are available within the leishmaniasis service in Pernambuco, although, there are no studies that validate them for co-infected HIV patients. A Brazilian study in 2006 11 using the same primers for PCR in the total blood of dogs, encountered a low sensitivity in this method and suggested the possibility of treating the low parasite load in the blood. The method used for DNA extraction, however, can have influenced the sensitivity and specificity of the PCR technique3.
This is the first study conducted in Brazil to investigate HIV-VL co-infection that describes the results of a set of diagnostic tools (parasitological, serological, molecular and urinary antigen detection). Only 14 patients were eligible for the study in more than one year. This low number of patients may be explained by the inclusion criteria based on the indication of bone marrow aspirate from the doctor´s assistant.
This series has included only five patients with co-infection and it is difficult therefore to analyze the results of the different diagnostic tests. In addition, there were cases of relapse, making even more difficult to reach reliable conclusions. Despite the small size of the sample, this article is a valuable addition to the limited literature on a topic that is of growing importance, both in Brazil and in other VL-endemic areas of the world.
From the clinical symptoms of the suspected or confirmed patients it was possible to observe that there are still difficulties in screening and diagnosing this particular group of patients. Thus, it may be perceived that there are limitations in these diagnostic methods and further studies may indicate which examinations are most appropriate for diagnosing and monitoring patients with HIV-VL co-infection.
Funding: This work was supported by grant APQ-1284-4.01/08 from the FACEPE (Foundation for Science and Technology of the State of Pernambuco) and PIBIC/CNPq (National Counsel of Technological and Scientific Development, Institutional Program for Scientific Initiation Scholarships), proposal contract No.: 109169/2009-3.
1. Alexandrino-de-Oliveira P, Santos-Oliveira JR, Dorval ME, Da-Costa FC, Pereira GR, da Cunha RV, et al. HIV/AIDS-associated visceral leishmaniasis in patients from an endemic area in Central-west Brazil. Mem Inst Oswaldo Cruz. 2010;105:692-7. [ Links ]
2. Alvar J, Aparicio P, Aseffa A, Den Boer M, Cañavate C, Dedet JP, et al. The relationship between leishmaniasis and AIDS: the second 10 years. Clin Microbiol Rev. 2008;21:334-59. [ Links ]
3. Alvar J, Cañavate C, Molina R, Moreno J, Nieto J. Canine leishmaniasis. Adv Parasitol. 2004;57:1-88. [ Links ]
4. Assis TS, Braga AS, Pedras MJ, Oliveira E, Barral A, de Siqueira IC, et al. Multi-centric prospective evaluation of rk39 rapid test and direct agglutination test for the diagnosis of visceral leishmaniasis in Brazil. Trans R Soc Trop Med Hyg. 2011;105:81-5. [ Links ]
5. Attar ZJ, Chance ML, El-Safi S, Carney J, Azazy A, El-Hadi M, et al. Latex agglutination test for the detection of urinary antigens in visceral leishmaniasis. Acta Trop. 2001;78:11-6. [ Links ]
6. Carranza-Tamayo CO, Assis TSM, Neri ATB, Cupolillo E, Rabello A, Romero GAS. Prevalence of Leishmania infection in adult HIV/AIDS patients treated in a tertiary-level care center in Brasilia, Federal District, Brazil. Trans R Soc Trop Med Hyg. 2009;103:743-8. [ Links ]
7. Centers for Disease Control. 1993 revised classification system for HIV infection and expanded surveillance case definition for AIDS among adolescents and adults. MMWR Recomm Rep. 1992;41(RR-17):1-19. [ Links ]
8. Chang MR, Taira CL, Paniago AMM, Taira DL, Cunha RV, Wanke B. Study of 30 cases of histoplasmosis observed in the Mato Grosso do Sul State, Brazil. Rev Inst Med Trop Sao Paulo. 2007;49:37-9. [ Links ]
9. Chappuis F, Rijal S, Singh R, Acharya P, Karki BMS, Das ML, et al. Prospective evaluation and comparison of the direct agglutination test and an rK39-antigen-based dipstick test for the diagnosis of suspected kala-azar in Nepal. Trop Med Int Health. 2003;8:277-85. [ Links ]
10. Daher EF, Fonseca PP, Gerhard ES, Silva Leitão TMJ, Silva Júnior GB. Clinical and epidemiological features of visceral leishmaniasis and co-infection in 15 patients from Brazil. J Parasitol. 2009;95:652-5. [ Links ]
11. Dantas-Torres F, Brandão-Filho SP. Expansão geográfica da leishmaniose visceral no Estado de Pernambuco. Rev Soc Bras Med Trop. 2006;39:352-6. [ Links ]
12. Ghatei MA, Hatam GR, Hossini MH, Sarkari B. Performance of latex agglutination test (KAtex) in diagnosis of visceral leishmaniasis in Iran. Iran J Immunol. 2009;6:202-7. [ Links ]
13. Gavgani AM, Vatan SK, Ghazanchaei A. KAtex antigen-detection test as a diagnostic tool for latent visceral leishmaniasis cases. Afr J Biotechnol. 2008;7:852-9. [ Links ]
14. Hailu A, Berhe N. The performance of direct agglutination tests (DAT) in the diagnosis of visceral leishmaniasis among Ethiopian patients with HIV co-infection. Ann Trop Med Parasitol. 2002;96:25-30. [ Links ]
15. Le Fichoux Y, Quaranta JF, Aufeuvre JP, Lelievre A, Marty P, Suffia I, et al. Occurrence of Leishmania infantum parasitemia in asymptomatic blood donors living in an area of endemicity in southern France. J Clin Microbiol. 1999;37:1953-7. [ Links ]
16. Lyons S, Veeken H, Long J. Visceral leishmaniasis and HIV in Tigray, Ethiopia. Trop Med Int Health. 2003;8:733-9. [ Links ]
17. Maia-Elkhoury ANS, Carmo EH, Sousa-Gomes ML, Mota, E. Análise dos registros de leishmaniose visceral pelo método de captura-recaptura. Rev Saude Pública. 2007;41:931-7. [ Links ]
18. Marques N, Cabral S, Sá R, Coelho F, Oliveira J, Saraiva da Cunha JG, et al. Leishmaniose visceral e infecção por vírus da imunodeficiência humana na era da terapêutica anti-retrovírica de alta eficácia. Acta Med Port. 2007;20:291-8. [ Links ]
19. Mira JA, Corzo JE, Rivero A, Macías J, De León FL, Torre-Cisneros J, et al. Frequency of visceral leishmaniasis relapses in human immunodeficiency virus-infected patients receiving highly active antiretroviral therapy. Am J Trop Med Hyg. 2004;70:298-301. [ Links ]
20. Molina I, Falcó V, Crespo M, Riera C, Ribera E, Curran A, et al. Efficacy of liposomal amphotericin B for secondary prophylaxis of visceral leishmaniasis in HIV-infected patients. J Antimicrob Chemother. 2007;60:837-42. [ Links ]
21. Osman OF, Oskam L, Zijlstra EE, Kroon NC, Schoone GJ, Khalil ET, et al. Evaluation of PCR for diagnosis of visceral leishmaniasis. J Clin Microbiol. 1997;35:2454-7. [ Links ]
22. Pasquau F, Ena J, Sanchez R, Cuadrado JM, Amador C, Flores J, et al. Leishmaniasis as an opportunistic infection in HIV-infected patients: determinants of relapse and mortality in a collaborative study of 228 episodes in a Mediterreanean region. Euro J Clin Microbiol Infect Dis. 2005;24:411-8. [ Links ]
23. Pastore RD, Chadburn A, Kripas C, Schattner EJ. Novel association of haemophagocytic syndrome with Kaposi's sarcoma-associated herpesvirus-related primary effusion lymphoma, Br J Haematol. 2000;111:1112-5. [ Links ]
24. Pintado V, López-Vélez R. HIV-associated visceral leishmaniasis. Clin Microbiol Infect. 2001;7:291-300. [ Links ]
25. Rabello A, Orsini M, Disch J. Leishmania/HIV co-infection in Brazil: an appraisal. Ann Trop Med Parasitol. 2003;97(Suppl 1):17-28. [ Links ]
26. Reyes Corcho A, Díaz Jidy M, Pérez Rodriguez A, Bouza Jiménez Y, Bouza Jiménez Y. Factores asociados con la presnecia de tuberculosis en pacientes con el syndrome de inmunodeficiencia adquirida en Cuba. Rev Panam Salud Publica. 2004;15: 341-7. [ Links ]
27. Riera C, Fisa R, Lopez P, Ribera E, Carrió J, Falcó V, et al. Evaluation of a latex agglutination test (KAtex) for detection of Leishmania antigen in urine of patients with HIV-Leishmania co-infection: value in diagnosis and post-treatment follow up. Eur J Clin Microbiol Infect Dis. 2004;23:899-904. [ Links ]
28. Roselino AM, Chociay MF, Costa RS, Machado AA, Figueiredo JFC. L. (L.) chagasi in AIDS and visceral leishmaniasis (kala-azar) co-infection. Rev Inst Med Trop Sao Paulo. 2008;50:251-4. [ Links ]
29. Sinha PK, Pandey K, Bhattacharya SK. Diagnosis & management of leishmania/HIV co-infection. Indian J Med Res. 2005;121:407-14. [ Links ]
30. Sundar S, Chakravarty J, Agarwal D, Rai M, Murray HW. Single-dose liposomal amphotericin B for visceral leishmaniasis in India. N Engl J Med. 2010;362:504-12. [ Links ]
31. Sundar S, Reed SG, Singh VP, Kumar PC, Murray HW. Rapid accurate field diagnosis of Indian visceral leishmaniasis. Lancet. 1998;351:563-5. [ Links ]
32. Ter Horst R, Collin SM, Ritmeíjer K, Bogale A, Davidson RN. Concordant HIV infection and visceral leishmaniasis in Ethiopia: the influence of antiretroviral treatment and other factors on outcome. Clin Infect Dis. 2008;46:1702-9. [ Links ]
33. Ter Horst R, Tefera T, Assefa G, Ebrahim AZ, Davidson RN, Ritmeijer K. Field evaluation of rK39 test and direct agglutination test for diagnosis of visceral leishmaniasis in a population with high prevalence of human immunodeficiency virus in Ethiopia. Am J Trop Med Hyg. 2009;80:929-34. [ Links ]
34. Vilaplana C, Blanco S, Domínguez J, Giménez M, Ausina V, Muñoz C. Noninvasive method for diagnosis of visceral leishmaniasis by a latex agglutination test for detection of antigens in urine samples. J Clin Microbiol. 2004;42:1853-4. [ Links ]
35. Zijlstra EE, Ali MS, El-Hassan AM, El-Toum IA, Satti M, Ghalib HW, et al. Kala-azar: a comparative study of parasitological methods and the direct agglutination test in diagnosis. Trans R Soc Trop Med Hyg. 1992;86:505-7. [ Links ]
Aracele Tenorio de Almeida e Cavalcanti.
Universidade de Pernambuco, Avenida Manoel Borba 361, Boa Vista, Recife, PE, Brasil.
Tel. +55.81.9262-2691. E-mail: email@example.com
Received: 24 July 2011
Accepted: 14 December 2011