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Revista da Sociedade Brasileira de Medicina Tropical

Print version ISSN 0037-8682

Rev. Soc. Bras. Med. Trop. vol.9 no.3 Uberaba May/June 1975

http://dx.doi.org/10.1590/S0037-86821975000300005 

Aspectos ultra-estruturais da forma epimastigota do Trypanosoma cruzi em meio LIT

 

 

Wanderley de SouzaI; Noemia Grynberg II; F. Nery-GuimarãesII

I Laboratório de Microscopia Eletrônica, Instituto de Biofísica U.F.R.J., Cidade Universitária, Rio de Janeiro - RJ
II Laboratório de Protozoologia, Instituto Oswaldo Cruz, Rio de Janeiro - RJ

 

 


RESUMO

E feito um estudo da ultra-estrutura da forma epismastigota do Trypanosoma cruzi mantida em meio de cultivo acelular.

O núcleo das formas em divisão apresenta um aspecto homogêneo. Microtúbulos intranucleares são observados durante a divisão. No entanto, a membrana nuclear permanece íntegra.

O citoplasma apresenta-se com vacúolos de dimensões e aspectos variados.

Com o método do ácido periódico-tiosemicarbazida-proteinato de prata, polissacaríáeos e/ou glicoproteínas foram localizados na membrana celular e na membrana que delimita certos vacúolos citoplasmáticos.


SUMMARY

In this paper the fine structure of the epimastigotes forms of Trypanosoma cruzi maintained in an acellular culture medium, is described.

During division the nucleus shows a homogenous structure owing to the dispersion of its chromatin and nucleolar material. Microtubules similar to those of a mitotic spindle in metazoan cells appear, running frorn one pole to the other. During the whole process of division the nuclear membrane remains intact.

The cytoplam shows many vacuoles. With the cytochemical method of acid periodic-thiosemicarbazide-silver proteinate, a polysaccharide surface coat is observed on the whole surface membrane system of T. cruzi. A positive re- action is also observed in the membranes of some vacuoles in the cytoplasm of the parasites.


 

 

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Recebido para publicação em 21.5.1975.

 

 

Lamentamos informar o falecimento do Prof. F. Nery-Guimarães em 15-4-1975. A sua amizade e a maneira com que se dedicava à investigação científica ficarão para sempre na memória de todos aqueles que com ele conviveram.

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