SciELO - Scientific Electronic Library Online

 
vol.15 issue1Anatomy and microanatomy of the reproductive system of Rectartemon (Rectartemon) depressus (Heynemann) (Mollusca, Gastropoda, Streptaxidae): the phallus complexThe microsculpture of the shell of Rectaktbmon (Rectíartemon) depressus (Heynemann) (Gastropoda, Stylomatophora, Streptaxidae) author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Revista Brasileira de Zoologia

Print version ISSN 0101-8175

Rev. Bras. Zool. vol.15 no.1 Curitiba  1998

http://dx.doi.org/10.1590/S0101-81751998000100008 

Mudanças morfológicas dos testículos de curimbatá Prochilodus scrofa (Steindachner) (teleostei, prochilodontidae), submetido à indução hormonal

 

Morphological changes of the testes of the curimbatá, Prochilodus scrofa (Steindachner), under hormonal stimulation

 

 

Emico Tahira Kavamoto; Massuka Yamane Narahara; Elaine Fender de Andrade-Talmelli

Divisão de Pesca Interior, Instituto de Pesca, CPA/SAA. Avenida Francisco Matarazzo 455, 05031-900 São Paulo, Brasil.

 

 


ABSTRACT

This Study Concerns the morphological changes of the testes of "curimbatá", Prochilodus scrofa (Steindachner, 1881) at the beginning of sperm production, before and after the hormonal injections. We examined 25 three year old males that were reared in tanks at the Experimental Fish and Frog Culturing Station of Pindamonhangaba of the Fisheries Institute of São Paulo. The testes were sampled at the start of sperm production, after the first hormone injection (5 IU/g for weight of hCG), and 14 days before and after a second injection. Testicle fragments of these specimens were fixed and embedded in historesin. At the beginning of sperm production, the milky-white gonad showed consistem superficial vascularization, exceeding thick sperm under light pressure. Microscopically, the presence of spermatogonia, many spermatocytes, spemiatids and some tubules with spermatozoa were conspicuous. After the first hormonal dose, the gonads increased in volume, occupying most of the abdominal cavity superficial blood irrigation was evident, and sperm flowed easily out of the genital pore. Many spermatozoa were observed in the histology sections, along with spermatocytes and spemiatids, within the lumen of the seminal tubules. After 14 days, the testes exhibited decreased volume and pink-white coloration. Light microscopy revealed few spermatozoa and disarrangement of the structures forming the seminal tubules. After the second hCG dose, the testes again increased in volume, occupying most of the abdominal cavity. Some tubules were filled with spermatozoa whereas others showed reduced numbers of this cellular type.

Key words: Prochilodus scrofa, fish, testes, morphology, hormonal stimulation


 

 

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

 

 

AGRADECIMENTOS. As autoras agradecem a Cleide S.R. Mainardes-Pinto, Chefe da Estação Experimental de Piscicultura e Ranicultura de Pindamonhangaba e aos funcionários de apoio à pesquisa pertencentes a esta Estação, pela colaboração na coleta de dados. Agradecem também, ao técnico José Plaza pela confecção das lâminas histológicas.

 

REFERÊNCIAS BIBLIOGRÁFICAS

Alexandrino, A.C.; MT. Phan & E.F.G. Pinheiro. 1985. Caracterização macroscópica e microscópica das gônadas do curimbatá, Prochilodus scrofa (Steindachner, 1881), durante o ciclo reprodutivo. Bolm. Zool. Univ. São Paulo 9:159-175.         [ Links ]

Billard, R.; J.P. Choisis & P. Reinaud. 1983. Stimulation of spermiation in carp in response to LH-RH and D-Ala6-LH-RH ethylamide. Aquaculture 35 (2):173-176.         [ Links ]

Billard, R; K. Bieniarz; W. Popek; P. Epler & A. Saad. 1989. Observations on a possible pherormonal stimulation of milt production in carp (Cyprinus carpio L.). Aquaculture 77 (4):387-392.         [ Links ]

Courtois, F.; F. Takashima & D.R. Billard. 1986. Stimulation of spermiation following repeated injection of carp pituitary homogenates in the carp. Bull. Jap. Soc. Sci. Fish 52 (6):995-997.         [ Links ]

Ciereszko, A.; L. Ramseyer & K. Dabrowski. 1996. Cryopreservation of Yellow Perch sêmen. Progressive Fish-Culturist 55:261-264.         [ Links ]

Kavamoto, E.T.; W.F. Silveira; H.M. Godinho & E. Romagosa. 1989. Fertilização em Prochilodus scrofa, Steindachner, 1881, com sêmen criopreservado em nitrogênio líquido. Bol. Inst. Pesca 16 (I):29-36.         [ Links ]

Kavamoto, E.T.; E.M. Ferraz; E.F. de Andrade-Talmelli; C.S.R. Mainardes-Pinto; E. Romagosa; M.Y. Narahara; R.C. Barnabe & B.E.S. de Campos. 1996. Estimulação da espermiação em curimbatá Prochilodus scrofa (Steindachner) através de aplicações de hCG (Osteichthyes, Characiformes, Prochilodontidae). Revta bras. Zool. 13 (1):27-38.         [ Links ]

Kobayashi, M.; K.L. Aida & I. Hanyu. 1986. Effeets of on milt amount and plasma levei of steroid hormones in male goldfish. Bull. Jap. Soc. Sci. Fish. 52 (4):755-756.         [ Links ]

Ohta, H. & T. Izawa. 1996. Diluent for cool storange of the japanese eel (Anguilla japonica) spermatozoa. Aquaculture 142:107-1 18.         [ Links ]

Saad, A. & R. Billard. 1987. Spermatozoa production and volume of semen collected after hormonal stimulation in the carp, Cyprinus carpio. Aquaculture, The Netherlands, 65 (1):67-77.         [ Links ]

Takashima, F.; C. Weill; R. Billard; L.W. Crim & A. Fostier. 1984. Stimulation of spermiation LH-RH analogue in carp. Bull. Jap. Soc. Sci. Fish. 50 (8):1329-1339.         [ Links ]

 

 

Recebido em 22.VIII.1996, aceito em 27.II.1998.

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License