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Scientia Agricola

On-line version ISSN 1678-992X

Sci. agric. (Piracicaba, Braz.) vol.60 no.3 Piracicaba  2003

https://doi.org/10.1590/S0103-90162003000300018 

PLANT PHYSIOLOGY AND BIOCHEMISTRY

 

Morphomolecular characterization of Pleurotus ostreatus (Jacq. Fr.) kummer strains in relation to luminosity and temperature of frutification

 

Caracterização morfomolecular de isolados de Pleurotus ostreatus (Jacq. Fr.) kummer em relação à luminosidade e temperatura de frutificação

 

 

Regina Helena Marino*; Augusto Ferreira da Eira; Eiko Eurya Kuramae; Elvio Cardoso Queiroz

UNESP/FCA - Depto. de Produção Vegetal, C.P.237 - 18603-970 – Botucatu, SP - Brasil

 

 


ABSTRACT

Temperature is one of the main factors affecting mushrooms development and introduction in new areas. Effects of temperature (15ºC and 28ºC) and luminosity (120 and 900 lux) were evaluated for eight P. ostreatus strains in relation to precocity, yield, pileus area, stalk formation pattern, coloration and handling resistance. Genetic variability of strains was analysed by the Random Amplified Polymorphic DNA (RAPD) method. The Pos 98/37 strain was the only to yield white pileus at 28ºC – 900 lux, and grey ones at 15ºC and 120 lux. The Pos 96/05 strain, the latest, produced lead-coloured pileus at 15ºC, as did the remaining strains at this temperature. Strains cultivated at 15ºC did not differ in relation to handling resistance. At 28ºC mushrooms were less resistant. In relation to yield, the Pos 98/38 strain was significantly more efficient. The Pos 98/37 strain, at 28ºC, as compared to the same strain at 15ºC, was more efficient and had an asymmetric stalk formation pattern. Among strains cultivated at 15ºC, the stalk formation pattern was symmetric, except for the Pos 97/15 and Pos 97/17 strains. Molecular characterization of the Pos 98/37 strain was 30% similar to the remaining strains. The temperature of fructification and luminosity influence the induction and development of the isolates.

Keywords: mushrooms, temperature, morphology of basidiocarps, yield and marked RAPD


RESUMO

A temperatura é um dos principais fatores que influenciam o desenvolvimento e a introdução de cogumelos em nova áreas. O efeito da temperatura (15ºC e 28ºC) e a luminosidade (120 e 900 lux) foram avaliados em oito isolados de P. ostreatus quanto à precocidade, eficiência biológica, área do pileus, padrão de formação das pencas, coloração e resistência ao manuseio. A variabilidade genética dos isolados foi analisada pelo método "Random Amplified Polymorphic DNA" ou DNA polimórfico amplificado ao acaso (RAPD). O isolado Pos 98/37 foi o único a produzir a 28ºC e 900 lux, apresentando píleo branco nessa temperatura e cinza a 15ºC e 120 lux. O isolado Pos 96/05, o mais tardio, apresentou píleo chumbo a 15ºC a 120 lux, assim como os demais isolados nesta temperatura. Os isolados cultivados a 15ºC não diferiram quanto à maior resistência ao manuseio, enquanto a 28ºC, os cogumelos obtidos foram mais frágeis. Quanto à eficiência biológica, o isolado Pos 98/38 foi mais eficiente. O isolado 98/37 a 28ºC, comparado com o mesmo isolado a 15ºC, foi mais eficiente e apresentou padrão de formação de pencas assimétrico. Entre os isolados cultivados a 15ºC, o padrão de formação de pencas foi similar exceto nos isolados Pos 97/15 e Pos 97/17. Na caracterização molecular, o isolado Pos 98/37 apresentou 30% de similaridade com os demais. A temperatura de frutificação e a intensidade luminosa influenciaram a indução e o desenvolvimento dos isolados.

Palavras-chave: cogumelos, temperatura de produção, morfologia dos basidiocarpos, eficiência biológica e marcador RAPD


 

 

INTRODUCTION

Pleurotus ostreatus is an edible fungus of great biotechnological interest, not only for its ability to grow on numerous agricultural residues to produce mushrooms of high organoleptical quality (Rajarathnam & Bano, 1987), but also because this fungus produces secondary metabolites with pharmaceutical applications and some proteins of industrial use - amino acids, vitamins, etc. These properties significantly increased its commercial value in the last years (Bunyard et al., 1996; Zervakis & Balis, 1996).

P. ostreatus is extensively produced out in localities with average temperature of 15ºC (Zadrazil, 1978). There is, however, a variety of P. ostreatus from Thailand, which grows in temperatures above 25ºC and is considered to be P. ostreatus var. Florida, described by Eger et al. (1976) and Eger (1978), since it presents cream-coloured to white pileutables, and sexual compatibility with the Japanese isolate of P. ostreatus (Kinugawa et al., 1997).

The coloration of the pileus is probably related to luminous intensity (Durand, 1976). The coloration of "Shiitake" mushrooms (Lentinula edodes) is a characteristic associated to the interactive effect of the temperature and luminous intensity (Przybylowicz & Donoghue, 1990).

The objective of this work was to describe the morphomolecular characteristics of P. ostreatus isolates in relation to luminosity and temperature in axenic culture, evaluating its precocity, biological efficiency, pileus area, stalk formation pattern, coloration, handling resistance and molecular characteristics of the isolates, using Random Amplified Polymorphic DNA (RAPD) method.

 

MATERIAL AND METHODS

Eight isolates of P. ostreatus (cultures covered with mineral oil) were used in this experiment carried out in Botucatu, SP, Brazil (22º53'09"S and 48º26'42"W) (Table 1). They were obtained from growers and from re-isolates of the based context of basidiocarp and, under aseptic conditions multiplied in culture media based on sawdust-dextrose-agar (SDA) and incubated at 25ºC for seven days (Eira & Minhoni, 1997).

The substrate consisted of sawdust (49.8%), wheat bran (20%), rice bran (20%), sugarcane bagasse (10%) and lime (0.2%). The mixture was conditioned in bottles, with the standardization of 400 and autoclaved at 120ºC during 4 hours. Inoculation was achieved through the transference of 0.5 cm diameter circle of culture medium (Eira & Minhoni, 1997) colonized in the bottles with the sterilized sawdust-based substrate. Inoculation was performed in an aseptic chamber and at the temperature of 25ºC for 30 days, without light.

After incubation, primordium induction was made by taking off the coverage and adding 2 to 3 cm of water during 4 hours, without thermal shock. Treatments consisted of, (1) 15 ± 5ºC, 120 lux (photoperiod of 12h) and 85 to 90% relative humidity and (2) 28 ± 5ºC, 900 lux (photoperiod of 24h) and 85 to 90% relative humidity, set in a completely randomized design (n = 10) (bottles with 400 of humid substrate). Parameters for analyses were: primordium formation and harvest (days), biological efficiency (grams of fresh mushrooms/grams of dry substrate x 100), coloration of the pileus at harvest (visual analysis), size of the harvested basidiocarps (photographic analysis) and handling resistance evaluated by the presence of breakable pileus (easily broken or firm).

Each isolate was photographed after the harvest with a 640 x 480 pixel resolution digital camera. Digital analysis evaluated pileus area, which was related to temperature of fructification, trying to establish a stalk formation pattern and the intensity of coloration of the pileus given by the number of pixels in the grey scale (0 - black to 250 - white), using the Image Tool program (Celso, 1999). The fructification pattern was studied through the analysis of frequency, asymmetry and curtose, criming to separate the isolates of greater similarity.

The process of differentiation of the genetic material of the isolates followed the RAPD method described by Williams et al. (1990). The genonic DNA was extracted as suggested by Sadowsky et al. (1987) and Kuramae-Izioka (1997). Amplification reactions were performed using a DNA thermal cycler (MJ Research), programmed according to Sambrook et al. (1989). The samples and the Ladder 1 Kb (Gibco BRL Life Technologies, Inc.) molecular marker were applied to 1.5% agarose gel using the TBE buffer (0.1 mol L-1 Tris-HCl; 0.1 mol boric acid L-1; 0.02 m mol L-1 EDTA; pH 8.0). The eletrocphoresis ran on approximately 120 V for 3 hours. The gel was photographed using ultraviolet light. The genetic similarity between the isolates was calculated using program Numerical Taxonomy and Multivariate Analysis System (NTSYS), with the coefficient "simple matching" (SM) and the construction of the dendrogram for the Unweighted Pair-Group method Arithmetic Average (UPGMA) method.

 

RESULTS AND DISCUSSION

The isolate of P. ostreatus cultivated at 15ºC was later in relation to the period of formation of primordium and harvest. Isolate Pos 98/37 was the only isolate that fruited at 28ºC (Table 2). The reduction of the metabolism of microorganisms under low temperature was already expected, as mentioned for Zadrazil (1978). The use of isolate of L. edodes not adapted to tropical regions does not form primordia, similarly to what occurred at 28ºC with isolates of P. ostreatus, except for Pos 98/37 (Chang et al., 1995). This behavior is credited to the use of isolates introduced by Japanese immigrants for the commercial production of P. ostreatus (Eira & Minhoni, 1997) mushrooms. Isolates from Japan are commercially cultivated at an average 15ºC (Kinugawa et al., 1997). Isolates of P. ostreatus var. Florida are adapted to an average 25ºC, and produce pileus cream to white coloured (Eger et al., 1976). Kinugawa et al. (1997) also recorded an isolate similar the P. ostreatus var. Florida in Thailand with these characteristics. In this work, isolate Pos 98/37 at 28ºC and 900 lux presented white pileus, as mentioned by Kinugawa et al. (1997) for isolate of P. ostreatus of Thailand and by Eger et al. (1976) for P. ostreatus var. Florida. At 15ºC and 120 lux (Table 2), all isolates presented pileus with colorations changing from grey to lead, same as observed by Eger et al. (1976) and Kinugawa et al. (1997) in studies with P. ostreatus at 15ºC.

Producing mushroom "Shiitake" at low temperature needs less luminosity than at high temperature (Przybylowicz & Donoghue, 1990). This behavior was also observed for isolate of P. ostreatus at 15ºC, when a luminous intensity of 120 lux was enough to induce the coloration of the pileus of all isolates. On the other hand, only the isolate that fruited at 28ºC (Pos 98/37), with the luminous intensity of 900 lux, did not present coloration of the pileus, remaining white (Table 2 and Figure 1). Similarly, in parallel experiments, P.ostreatus activated at with luminous intensity of 120 lux and 28ºC did not present pileus coloration. But at 15ºC with luminous intensity of 900 lux, the eight isolates of P. ostreatus presented reduction of coloration with the increase of the luminosity (results not presented), evidencing the importance of the interaction temperature vs. isolate vs. luminous intensity. This color variation is very frequent in the species and depends on environmental conditions, as reported by Rajarathnam & Bano (1987) and Eger et al. (1974).

 

 

Edible mushrooms cultivated between 15-20ºC present better quality and durability than those at 25ºC (Eger et al., 1976; Przybylowicz & Donoghue, 1990). An example was observed in this work in relation to the handling resistance after harvest (Table 2).

Isolate Pos 98/37 presented greater biological efficiency (EB) at 28ºC than at 15ºC. The isolates Pos 97/15 and Pos 98/38 presented, at 15ºC, respectively, the lowest and the highest values of EB. However, isolate Pos 98/38 was harvested after the established commercial pattern what, in part, can explain the high values of EB (Table 2). The commercial pattern of mushrooms with pileus area up to 150 mm2 increases their value. In this experiment, isolates Pos 98/37, Pos 98/38 and Pos 98/40 at 15ºC and Pos 98/37 at 28ºC were harvested after reaching the commercial pattern (Figure 2).

 

 

The fructification temperature influenced the pattern of stalk formation of mushrooms, therefore isolate Pos 98/37 cultivated at 28ºC presented greater formation of primordium as compared to 15ºC. Isolates Pos 97/15, Pos 97/17 and Pos 98/40 also presented high concentration of primordium, with uniform distribution of mushrooms in relation to stalk formation pattern and area. In a similar way, isolate Pos 98/38, harvested after reaching the commercial pattern, presented uniform stalk formation patterns (Figure 2). Pos 98/37 of P. ostreatus (white variety and the only to fructify at 28ºC), presented polymorphism with 30% of genetic similarity in relation to isolated adapted to 15ºC for production (Figure 3). Isolate Pos 98/37 also was certified as P. ostreatus (Meijer1, 2000).

 

 

The presence of polymorphism and minor similarity between isolates Pos 98/37 and all other, is probably related to the existence of genes important to the tolerance to high temperatures, as observed by Li (1980) and cited by Müller (1988) for some isolates of P. ostreatus, which have seven dominant genes, inherited from generation to generation, related to the resistance to heat (Guinberteau et al., 1991; Callac et al., 1998) report the presence of genes responsible for the induction of primordium and other genes related to the coloration of the basidiocarps. These authors indicated that the genetic control of the pileus coloration is given by a monogenic, dominant factor, while the white color or without coloration is related to recessive alleles. However, considering pileus coloration, the temperature effect has also to be taken into consideration since isolate Pos 98/37, cultivated at 28ºC had white pileus, but at 15ºC became grey. Thus, the genetic manifestation of each isolate is influenced by temperature and luminous intensity (qualitative evaluation of the isolates), leaving aside other factors not studied herein.

On the other hand, as observed by Larraya et al. (1999), the other isolates of P. ostreatus were similar, indicating low genetic variability, which can result in a production risk, specially, in cases of biotic or abiotic stress. Therefore, among the isolates used in this experiment, Pos 98/37 have great importance for genetic improvement programs which aim precocious and heat tolerant isolates, behaving with commercially desirable morphologic characteristics, such as those described by Pahil et al. (1991), for an isolate of Agaricus bitorquis that, in addition to fructifying at 25ºC, presented good commercial quality.

 

ACKNOWLEDGMENTS

To FAPESP, Project 97/14675-5.

 

REFERENCES

BUNYARD, B.A.; CHAICHUCHOTE, S.; NICHOLSON, M.S.; ROYSE, D.J. Ribosomal DNA analysis for resolution of genotypic classes of Pleurotus. Mycological Research, v.2, p.143-150, 1996.        [ Links ]

CALLAC, P.; MOQUER, F.; IMBERNON, M.; GUEDES-LAFARGUE, M.R.; MAMOUN, M.; OLIVIER, J.M. Evidence for PPC1, a determinant of the pilei-pellis color of Agaricus bisporus fruitbodies. Fungal Genetics and Biology, v.23, p.181-188, 1998.        [ Links ]

CELSO, P.G. Interações entre Agaricus bisporus e microrganismos termófilos isolados do substrato de cultivo do cogumelo. Araraquara: UNESP/IQ, 1999. 83p. (Dissertação - Mestrado)        [ Links ]

CHANG, S.T.; KWAN, H.S.; KANG, Y.N. Colletion, characterization, and utilization of germ plasm of Lentinula edodes. Canadian Journal of Botany, n.73, p.955-961, 1995, Supplement 1.        [ Links ]

DURAND, R. Influence des radiations luminuses sur les processus de reproduction des champignons: hypotheses sur lidentite des photorecepteurs: revue bibliographique. Mycologia, v.60, p.3-16, 1976.        [ Links ]

EGER, G.; GOTTWALD, H. D.; VON NETZER, U. The action of light and other factors on sporophore initiation in Pleurotus ostreatus. Mushroom Science, v.9, p.575-583, 1974.        [ Links ]

EGER, G.; EDEN, G.; WISSING, E. Pleurotus ostreatus, breeding potential of a new cultivated mushroom. Theoretical and Applied Genetics, v.47, p.155-163, 1976.        [ Links ]

EGER, G. Biology and breeding of Pleurotus. In: CHANG, S.T.; HAYES, W.A. (Ed.) The biology and cultivation of edible mushrooms. New York: Academic Press, 1978. cap.24, p.497-519.        [ Links ]

EIRA, A.F.; MINHONI, M.T.A. Manual teórico-prático do cultivo de cogumelos comestíveis. 2.ed. Botucatu: Fundação de Pesquisa Agropecuária e Florestais, 1997. 115p.        [ Links ]

GUINBERTEAU, J.; OLIVIER, J.M.; TANNE, M.N. Improvement of Lepista species cultivation, technical factors, and selection of strains. Mushroom Science, v.2, p.615-621, 1991.        [ Links ]

KINUGAWA, K.; TANESAKA, E.; NAGATA, A.; WATANABE, K. Cross-compatibility between Thai and Japanese Oyster mushrooms and the inheritance of fruiting habits. Memoirs of the Faculty of Agriculture of Kinki University, n.30, p.7-11, 1997.        [ Links ]

KURAMAE-IZIOKA, E.E. A rapid, easy and high yield protocol for total genomic DNA isolation of Colletotrichum gloesporioides and Fusarium oxysporum. Revista Unimar, v.19, p.683-689, 1997.        [ Links ]

LARRAYA, L.; PEÑAS, M.M.; PÉREZ, G.; SANTOS, C.; RITTER, E.; PISABARRO, A.G.; RAMÍREZ, L. Identification of incompatibility aleles and characterisation of molecular markers genetically linked to the A incompatibility locus in the white rot fungus Pleurotus ostreatus. Current Genetic, v.34, p.486-493, 1999.        [ Links ]

MÜLLER, J. Genetic potencial of Pleurotus ostreatus: relevance to the disposal of agro-wastes. Micological Neotropical Aplicada, v.1, p.29-44, 1988.        [ Links ]

PAHIL, V.S., SMITH, J.F.; ELLIOTT, T.J. The testing and improvement of high temperature, wild Agaricus strains for use in tropical and sub-tropical climates. Mushroom Science, v.2, p. 589-599, 1991.        [ Links ]

PRZYBYLOWICZ, P.; DONOGHUE, J. Shiitake growes handbook. 2.ed. New York: Hunt Publishing Co, 1990. 217p.        [ Links ]

RAJARATHNAM , S.; BANO, Z. Pleurotus mushrooms. Parte 1 A: Morfology, life cycle, taxonomy, breeding and cultivation. CRR Critical Recviews in Food Science, v.26, p.157-223, 1987.        [ Links ]

SADOWSKY, M.J.; TULLY, R.E.; CREGAN, P.B.; KEYSER, H.H. Genetic diversity in Bradyrhizobium japonicum serogroup 123 and relation to genotype specific nodulation of soybean. Appllied and Environmental Microbiolology, v.53, p.2624-2630, 1987.        [ Links ]

SAMBROOK, J.; FRITSCH, E.F.; MANIATIS, T. Molecular cloning: a laboratory manual. 2.ed. New York: Academic Press, 1989.        [ Links ]

WILLIAMS, J.G.; KUBELIK, A.R.; LIVAK, K.J.; RAFALSKI, J.A.; TINGEY, S.V. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research, v.18, p.6531-5, 1990.        [ Links ]

ZADRAZIL, F. Cultivation of Pleurotus. In: CHANG, S.T.; HAYES, W.A. (Ed.) The biology and cultivation of edible mushrooms. New York: Academic Press, 1978. cap.25, p.521-557.        [ Links ]

ZERVAKIS, G.; BALIS, C. A pluralistic approach in the study of Pleurotus species with emphasis on compatibility and physiology of the European morphotaxa. Mycological Research, v.10, p.717-731, 1996.        [ Links ]

 

 

Received January 04, 2002
Accepted June 10, 2003

 

 

* Corresponding author <rehmarino@bol.com.br>

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