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• Bol. Inst. Oceanogr. 38 (1) • 1990 • https://doi.org/10.1590/S0373-55241990000100005   copy

Electrophoretic study on intraspecific variations and interspecific relationships of marine catfishes (Siluriformes, Ariidae) of Cananéia (São Paulo, Brazil): 1. General proteins of eye-lens and skeletic muscle

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstracts

Cellulose acetate electrophoresis of eye-lens proteins and Polyacrylamide flat gel electrophoresis of skeletic muscle proteins of six species of marine catfishes were carried out. Genetic polymorphism only occured at one locus of the electropherograms of eye-lens of Cathorops spixii. Ontogenetic variations in the relative concentration of bands were found in the electropherograms of eye-lens and skeletic muscle proteins. The six species of catfishes can be identified by means of quantitative and qualitative differences in the electropherograms. Coefficients of similarity were determined by the band-counting method and UPGMA dendrograms were constructed to illustrate the interspecific relationships among the species.

Electrophoresis; Proteins; Muscles; Eye-lens; Interspecific relationships; Ariidae; Cananéia; Brazil

Eletroforeses de proteínas gerais de cristalinos e de músculo esquelético de seis espécies de bagres marinhos foram realizadas, respectivamente, em membranas de acetato de celulose e em géis de poliacrilamida. Polimorfismo genético ocorreu apenas em um locus de eletroferogramas do cristalino de Cathorops spixii. Variações ontogenéticas nas concentrações relativas das bandas foram observadas nos eletroferogramas do cristalino e do músculo esquelético. As seis espécies de bagres marinhos podem ser identificadas através das diferenças quantitativas e qualitativas nos eletroferogramas. Coeficientes de similaridade foram determinadas pelo método de contagem de bandas e dendrogramas UPGMA foram construídos para ilustrar as relações interespecíficas entre as espécies.

Eletroforese; Proteínas; Músculos; Cristalino; Relações interespecíficas; Ariidae; Cananéia

ARTIGOS

Electrophoretic study on intraspecific variations and interspecific relationships of marine catfishes (Siluriformes, Ariidae) of Cananéia (São Paulo, Brazil). 1. General proteins of eye-lens and skeletic muscle

Hana Suzuki; Phan Van Ngan

Instituto Oceanográfico da Universidade de São Paulo (Caixa Postal 9075, 01051 São Paulo, SP)

ABSTRACT

Cellulose acetate electrophoresis of eye-lens proteins and Polyacrylamide flat gel electrophoresis of skeletic muscle proteins of six species of marine catfishes were carried out. Genetic polymorphism only occured at one locus of the electropherograms of eye-lens of Cathorops spixii. Ontogenetic variations in the relative concentration of bands were found in the electropherograms of eye-lens and skeletic muscle proteins. The six species of catfishes can be identified by means of quantitative and qualitative differences in the electropherograms. Coefficients of similarity were determined by the band-counting method and UPGMA dendrograms were constructed to illustrate the interspecific relationships among the species.

Descriptors: Electrophoresis, Proteins, Muscles, Eye-lens, Interspecific relationships, Ariidae, Cananéia, Brazil

RESUMO

Eletroforeses de proteínas gerais de cristalinos e de músculo esquelético de seis espécies de bagres marinhos foram realizadas, respectivamente, em membranas de acetato de celulose e em géis de poliacrilamida. Polimorfismo genético ocorreu apenas em um locus de eletroferogramas do cristalino de Cathorops spixii. Variações ontogenéticas nas concentrações relativas das bandas foram observadas nos eletroferogramas do cristalino e do músculo esquelético. As seis espécies de bagres marinhos podem ser identificadas através das diferenças quantitativas e qualitativas nos eletroferogramas. Coeficientes de similaridade foram determinadas pelo método de contagem de bandas e dendrogramas UPGMA foram construídos para ilustrar as relações interespecíficas entre as espécies.

Descritores: Eletroforese, Proteínas, Músculos, Cristalino, Relações interespecíficas, Ariidae, Cananéia: SP.

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Acknowledgements

We are grateful to Dr. Vicente Gomes and Mrs. Maria José A. C. R. Passos for their aid in sampling and laboratory analyses. This work was supported by grants from FAPESP (80/1694-0) and CNPq (10.1739/82 and 40.0528/82).

(Received 17-Nov-88; accepted 08-Aug-90)

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Publication Dates

  • Publication in this collection
    18 May 2012
  • Date of issue
    1990

History

  • Received
    17 Nov 1988
  • Accepted
    08 Aug 1990
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