ABSTRACT
Salinity is an important abiotic factor since it is responsible for the local and/or regional distribution of algae. In coastal regions, salinity changes with prevailing winds, precipitation and tide, and particularly in extreme intertidal conditions. Acanthophora spicifera is a red seaweed that occurs in the supratidal region in which changes in abiotic conditions occur frequently. This study evaluated the effects of salinity on the metabolism and morphology of A. spicifera. Algae were acclimatized under culture conditions with sterilized seawater for seven days. Experiments used different salinities (15 to 50 psu) for seven days, followed by metabolic analyses. This study demonstrates that extreme salinities affect physiological parameters of A. spicifera, such as decrease in growth rate, as well as morphological parameters and concentrations of secondary metabolites. Acanthophora spicifera exhibited high tolerance to 25 to 40 psu, with little change in physiology, which favors the occurrence of this species in diverse environments. However, 15, 20, 45 and 50 psu were the most damaging and led to loss of biomass, depigmentation of apices, and the highest concentrations of antioxidant metabolites. The 50 psu treatment caused the greatest changes in general, greatly reducing a biomass and chlorophyll content, and facilitating the presence of endophytes.
Keywords
Acanthophora spicifera; microscopy; morphology; physiology; salinity
Introduction
Many abiotic factors are important to aquatic ecosystems, including, for example, temperature, irradiance, nutrients, pH and salinity. Each factor can uniquely affect the metabolism of organisms. Salinity is particularly important, especially for algae, since it is responsible for local and/or regional distribution (Yarish & Kirkman 1990Yarish C, Kirkman H. 1990. Seaweeds: their environment, biogeography, and ecophysiology. Nova Jersey, John Wiley & Sons.; Nejrup et al. 2012Nejrup LB, Pedersen MF, Nejrup LB, Pedersen MF. 2012. The effect of temporal variability in salinity on the invasive red alga Gracilaria vermiculophylla. European Journal of Phycology 47: 254-263. ). Salinity tends to be a fairly stable abiotic factor in oceanic waters, but in the coastal region where red algae are found, this factor changes with prevailing winds, precipitation, and tides (Lartigue et al. 2003Lartigue J, Neill A, Hayden BL, et al. 2003. The impact of salinity fluctuations on net oxygen production and inorganic nitrogen uptake by Ulva lactuca (Chlorophyceae). Aquatic Botany 75: 339-350. ; Nejrup et al. 2012Nejrup LB, Pedersen MF, Nejrup LB, Pedersen MF. 2012. The effect of temporal variability in salinity on the invasive red alga Gracilaria vermiculophylla. European Journal of Phycology 47: 254-263. ). Since salinity affects osmotic adjustment and turgor pressure regulation, it is an important abiotic factor in the marine environment for the maintenance and survival of algae (Kirst 1990Kirst GO. 1990. Salinity tolerance of eukaryotic marine algae. Annual Review of Plant Biology 41: 21-53.).
According to the IPCC (2012IPCC - Intergovernmental Panel on Climate Change. 2012. Managing the risks of extreme events and disasters to advance climate change adaptation. In: Field CB, Barros V, Stocker TF, et al. (eds.) A special report of working groups I and II of the intergovernmental panel on climate change. Cambridge, New York, Cambridge University Press. p. 255-256. ), rainfall is expected to become very irregular in South America. Increased rainfall is expected in southeastern and southern Brazil, Paraguay, Uruguay, Argentina and some regions of Bolivia (Scherner et al. 2013Scherner F, Ventura R, Barufi JB, Horta PA. 2013. Salinity critical threshold values for photosynthesis of two cosmopolitan seaweed species: Providing baselines for potential shifts on seaweed assemblages. Marine Environmental Research 91: 14-25. ), leading to a change in the abiotic factors of aquatic ecosystems, including salinity. Fluctuations in salinity can change the density of water, nutrient uptake, and osmotic pressure in plant cells (Lobban et al. 1994Lobban CS, Harrison PJ, Duncan MJ. 1994. Light and photosynthesis. Seaweed Ecology and Physiology 123-162.; Fong et al. 1996Fong P, Boyer KE, Desmond JS, Zedler JB. 1996. Salinity stress, nitrogen competition, and facilitation: what controls seasonal succession of two opportunistic green macroalgae?. Journal of Experimental Marine Biology and Ecology 206: 203-221.), leading to significant physiological and biochemical stress for algae, such as changes in growth rate, photosynthetic performance, morphology, and germination in the green microalga Nannochloropsis salina (Bartley et al. 2013Bartley ML, Boeing WJ, Corcoran AA, et al. 2013 Effects of salinity on growth and lipid accumulation of biofuel microalga Nannochloropsis salina and invading organisms. Biomass and Bioenergy 54: 83-88.) and thalli of the red algae Pterocladiella capillacea (Felix et al. 2014Felix MR, Osorio LKP, Ouriques LC, et al. 2014. The effect of cadmium under different salinity conditions on the cellular architecture and metabolism in the red alga Pterocladiella capillacea (Rhodophyta, Gelidiales). Microscopy and Microanalysis 20: 1411-24. ), Stylonema alsidii (Stylonematophyceae) (Nitschke et al. 2014Nitschke U, Karsten U, Eggert A. 2014. Journal of experimental marine biology and ecology physiological performance of the red alga Stylonema alsidii (Stylonematophyceae) under varying salinities. Journal of Experimental Marine Biology and Ecology 460: 170-176. ), and Kappaphycus alvarezii (Mandal et al. 2015Mandal SK, Ajay G, Monisha N, et al. 2015. Differential response of varying temperature and salinity regimes on nutrient uptake of drifting fragments of Kappaphycus alvarezii: implication on survival and growth. Journal of Applied Phycology 27: 1571-1581. ). For red algae spores treated with different salinity, the development of the Gelidium floridanum was inhibited under hyposaline conditions, but only delayed under hypersaline conditions (Filipin et al. 2016Filipin EP, Bouzon ZL, Ouriques L, et al. 2016. Evaluation of salinity effects on the release, adhesion, and germination of the tetraspores of Gelidium floridanum (Rhodophyta, Florideophyceae). Journal of Applied Phycology 28: 2925-2938. ). However, studies of thalli and spores have generally yielded little information about the response of secondary metabolites relative to their antioxidant activity when salinity changes.
Acanthophora spicifera is a red seaweed found in tropical and subtropical regions (Kilar & Mclachlan 1986Kilar JA, Mclachlan J. 1986. Ecological studies of the alga, Acanthophora spicifera (Vahl) Barrg. (Ceramiales : Rhodophyta): vegetative fragmentation. Journal of Experimental Marine Biology and Ecology 104: 1-21.). It is native to the Caribbean and Florida (Horn 2012Horn RA. 2012. The effect of litopenaeus stylirostris aquaculture on macroalgae growth in opunohu Bay, moorea, French Polynesia. Student Research Papers 1: 1-12.), and it is exotic in Brazil, occurring from Maranhão State to Rio Grande do Sul (Fig. 1A). This species occurs in the supratidal region where changes in abiotic factors are constant. Little information about the phenotypic plasticity of this alga is available, but in Florianópolis, Santa Catarina, Brazil (Fig. 1A), it is found in two distinct environments: Conceição Lagoon and Sambaqui Beach. Conceição Lagoon (27º34’S; 48º27’W) is a small coastal lagoon located in the easternmost central part of Santa Catarina, connecting with the sea through a channel. It receives drainage of small freshwater flows and the Capivara River to the north, with consolidated substrate, and it is the biggest lagoon in the region (Souza-Mosimann et al. 2011Souza-Mosimann RM, Laudares-Silva R, Talgatti D, et al. 2011. The diatom flora in Conceição Lagoon, Florianópolis, SC, Brazil. Ínsula Revista de Botânica 40: 25-54.). Sambaqui Beach (27°29’S; 48°32’W) is located in the north bay of Florianópolis. It is part of the channel between the island and the mainland. Analyses performed in our laboratory indicate that Conceição Lagoon waters possesses on average 1.06 ± 0.27 µM NH4 + (ammonia), 8.47 ± 0.01 µM NO3 - (nitrate), 0.17 ± 0.01 µM PO4 -3 (phosphate), and 25 practical salinity unit (psu). For Sambaqui Beach, these values were 1.13 ± 0.05 µM NH4 +, 3.73 ± 0.01 µM NO3 -, 0.52 ± 0.01 µM PO4 -3, and 30 psu. However, measurements carried out by our research group show that the salinity at this site reached up to 37 psu, in summer. This indicates that this species can survive in quite different conditions, taking into account salinity and soluble nutrients.
A. Map with the occurrence of A. spicifera, from Maranhão State (red line) to Rio Grande do Sul (red line), and the location of both sites on Santa Catarina Island where the seaweed occurs: Conceição Lagoon and Sambaqui Beach. B. Macroalga A. spicifera collected at Sambaqui Beach with red-brownish color, typical of the species. Stipe branched erect alternating and abundant and covered by numerous short papillose branches, which have four to five short spinescent tips (magnifying).
Acanthophora spicifera presents a red-brownish or greenish color, grows in tufts of 8 to 10 cm and has branched erect branches alternately and abundantly (Fig. 1B) (Cordeiro-Marino 1978Cordeiro-Marino M. 1978. Rodentíceas marine bentônicas of the State of Santa Catarina. Instituto de Botânica 7: 1-243.). These branches are covered by numerous short papillose branches, which have 4 to 5 short spinescent tips (Cordeiro-Marino 1978Cordeiro-Marino M. 1978. Rodentíceas marine bentônicas of the State of Santa Catarina. Instituto de Botânica 7: 1-243.) (Fig. 1B). A. spicifera has a three-phase and isomorphic life cycle. The sexed phase is followed by two asexual generations that produce spores, termed carposporophytic and tetrasporophytic generations (Cordeiro-Marino 1978Cordeiro-Marino M. 1978. Rodentíceas marine bentônicas of the State of Santa Catarina. Instituto de Botânica 7: 1-243.). Several compounds of interest have been identified in A. spicifera, including flavonoids with antibacterial (Seenivasan et al. 2012Seenivasan R, Rekha M, Indu H, et al. 2012. Antibacterial activity and phytochemical analysis of selected seaweeds from Mandapam Coast, India. Journal of Applied Pharmaceutical Science 2: 159-169.), antitumor (Lavakumar et al. 2012Lavakumar V, Ahamed KFH, Ravichandran V. 2012. Anticancer and antioxidant effect of Acanthophora spicifera against EAC induced carcinoma in mice. Journal of Pharmacy Research 5: 1503-1507.), procoagulant and antioxidant activities (Zeng et al. 2001Zeng L-M, Wang C-J, Su J-Y, et al. 2001. Flavonoids from the red alga Acanthophora spicifera. Chinese Journal of Chemistry 19: 1097-1100.). Phenolic compounds with antiproliferative (Murugan & Iyer 2014Murugan K, Iyer VV. 2014. Antioxidant and antiproliferative activities of extracts of selected red and brown seaweeds from the Mandapam Coast of Tamil Nadu. Journal of Food Biochemistry 38: 92-101.) and antioxidant activities were also identified (Ganesan et al. 2008Ganesan P, Kumar CS, Bhaskar N. 2008. Antioxidant properties of methanol extract and its solvent fractions obtained from selected Indian red seaweeds. Bioresource Technology 99: 2717-2723. ). This species is also used as a biofilter (Fialho 2013Fialho FAN. 2013. Integrated multitrophic aquaculture: macroalgae biofilters in the culture of the arrowhead. Monograph, Universidade Federal de Santa Catarina, Florianópolis. ) and as a foodstuff in the human diet (Lang 2007Lang KL, Palermo JA, Falkenberg M, Schenkel EP. 2007. Steroids from the red alga Acanthophora spicifera. Biochemical Systematics and Ecology 35: 805-808. ; Zakaria et al. 2011Zakaria NA, Ibrahim D, Shaida SF, Supardy NA. 2011. Phytochemical composition and antibacterial potential of hexane extract from malaysian red algae, Acanthophora spicifera (Vahl) Borgesen. World Applied Sciences Journal 15: 496-501.). It is also a food source for fish, crabs and green turtles (Chelonia mydas) (Lang 2007Lang KL, Palermo JA, Falkenberg M, Schenkel EP. 2007. Steroids from the red alga Acanthophora spicifera. Biochemical Systematics and Ecology 35: 805-808. ). A. spicifera is also a producer of agarana (Duarte et al. 2004Duarte MER, Cauduro JP, Noseda DG, et al. 2004. The structure of the agaran sulfate from Acanthophora spicifera (Rhodomelaceae, Ceramiales) and its antiviral activity. Relation between structure and antiviral activity in agarans. Carbohydrate Research 339: 335-347. ), a sulphated polysaccharide of great economic value.
Therefore, this work aimed to determine the effect of salinity on the growth rate, concentration of photosynthetic pigments, as well as structure and concentration of secondary metabolites, phycobiliproteins, carotenoids, phenolics and flavonoids of the macroalga A. spicifera. It is anticipated that these data will improve our understanding of the present distribution of this species and allow projection of its future distribution.
Materials and methods
Collection sites, seawater and algal material
The experiments were performed with seaweeds collected at Sambaqui Beach, Florianópolis (Santa Catarina Island, Brazil). Sambaqui Beach (27°29’S; 48°32’W) is located in the north bay of Florianópolis. It is part of the channel between the island and the mainland. Tetrasporophytic samples of Acanthophora spicifera (M. Vahl) Børgesen (80 g of total thallus) attached to rocks were collected from Sambaqui Beach in December 2014 during the Southern Hemisphere summer season. Collection was made in the intertidal zone during low tide in the morning. The specimens were transported at ambient temperature in dark containers to the Laboratório de Biologia Celular Vegetal (Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, Brazil, and macroepiphytes were meticulously eliminated by cleaning with a brush and washing with filtered seawater. Thalli were maintained in culture medium with sterilized seawater from the site containing von Stosch medium with MnCl2, EDTA, NaHPO4, FeSO4, biotin, cyanocobalamin, thiamine and nitrate (Edwards 1972Edwards P. 1972. Cultured red alga to measure pollution. Marine Pollution Bulletin 3: 184-88.) and cultivated under laboratory-controlled conditions, including 24 ± 2 °C, continuous aeration, 70 ± 10 µmol photons m-2s-1 (fluorescent lamps, Philips C-5 Super 84 16W/840), and 12 h photocycle (from 08:00 to 20:00) during seven days for acclimation before experi mental treatments. The sterilized seawater was renewed every two days. PAR was measured with a Solar Light PMA 2100 quantameter (Solar Light Co., Glenside, PA, USA) and a Solar Light 2132 spherical sensor (Solar Light Co.).
Experimental setup
After acclimation, thalli (± 4.0 g fresh weight (FW) ± 8 individuals per beaker) of A. spicifera were cultivated for seven days in beakers containing 500 mL of respective sterilized seawater. Culture conditions were the same as those described for the acclimation period. Low salinities were obtained by addition of distilled water, while high salinities were attained through gradual freezing and thawing of seawater until the final concentration was reached. Four replicates were used for each experimental group (15, 20, 25, 30, 35, 40, 45 and 50 psu). The reference value was 30 psu because this is the salinity observed in the seawater of Sambaqui Beach. Culture conditions were the same as those described for the acclimation period. At the end of the experiments, some of the algae were photographed for analysis of external morphology, and the rest was frozen for cytological and metabolic analysis.
Morphological features
At the end of the experiments, the algae were photographed with a digital camera (Sony Dsc W-620 14.1 Megapixels) for analysis of external morphology.
Growth rate
Growth rates (n = 4) were calculated using the following equation: Growth rates [% day-1] = [(Wt/Wi)1/t - 1] x 100, where Wi = initial fresh weight (FW), Wt = fresh weight after seven days, and t = experimental time in days (Penniman et al. 1986Penniman CA, Mathieson AC, Emerich Penniman C. 1986. Reproductive phenology and growth of Gracilaria tikvahiae McLachlan (Gigartinales, Rhodophyta) in the great bay estuary, New Hampshire. Botanica Marina 29: 147-154.).
Photosynthetic pigments
At the end of the experimental period, fresh samples were frozen by immersion in liquid nitrogen and kept at -80 ºC until ready for use. All pigments were extracted in quadruplicate for determination of chlorophyll a and phycobiliprotein contents.
Chlorophyll a was extracted from approximately 1 g dry weight (DW) with 3 mL DMSO at 40 ºC for 30 min, using a glass tissue homogenizer (Hiscox & Israelstam 1979Hiscox JT, Israelstam GF. 1979. A method for the extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany 57: 1332-1334.; Schmidt et al. 2010Schmidt ÉC, Maraschin M, Bouzon ZL. 2010. Effects of UVB radiation on the carragenophyte Kappaphycus alvarezii (Rhodophyta, Gigartinales): changes in ultrastructure, growth, and photosynthetic pigments. Hydrobiologia 649: 171-182.). The homogenates were centrifuged at 2000 x g for 20 min, and pigments were quantified from the supernatant in a spectrophotometer (Hitachi, Model 100-20; Hitachi Co., Japan) at 630, 647 and 664 nm. Chlorophyll a concentrations were calculated according to Jeffrey & Humphrey (1975Jeffrey ST, Humphrey GF. 1975. New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural phytoplankton. Biochemie und Physiologie der Pflanzen.).
Phycobiliproteins were extracted from about 1 g FW ground to a powder with liquid nitrogen and 3 mL 0.05 M phosphate buffer, pH 6.4, at 4 ºC in darkness. The homogenates were centrifuged at 2,000 g for 20 min. Levels of allophycocyanin (APC), phycocyanin (PC), and phycoerythrin (PE) were determined by spectrophotometry (Hitachi, Model 100-20; Hitachi Co., Japan) at 498, 615 and 651 nm, and concentrations were calculated using the equations of Kursar & Alberte (1983Kursar TA, Alberte RS. 1983. Photosynthetic unit organization in a red alga relationships between light-harvesting pigments and reaction centers. Plant Physiology 72: 409-414.).
The extraction of carotenoids was performed according to Aman et al. (2005Aman R, Carle R, Beifuss U, Schieber A. 2005. Isolation of carotenoids from plant materials and dietary supplements by high-speed counter-current chromatography. Journal of Chromatography A 1074: 99-105. ). Carotenoids were extracted from samples (1.0 g FW, n=4) using methanol 80 %. After maceration, the samples were kept at rest (1 h) in a darkroom. The recovered extract was centrifuged at 2000 x g for 10 min, and total carotenoids were quantified from the supernatant in a spectrophotometer (Hitachi, Model 100-20; Hitachi Co., Japan) at 450 nm.
Total phenolic and flavonoid compounds
Analyses of phenolic compounds were performed using the spectrophotometric method of Folin-Ciocalteau based on Popova et al. (2007Popova M, Bankova VS, Bogdanov S, et al. 2007. Chemical characteristics of poplar type propolis of different geographic origin. Apidologie 38: 306-311.). Polyphenolics were extracted from frozen samples of 1.0 g DW (n = 4) by using methanol 80 % maintained in a darkroom for 1 h. In a test tube, 300 mL of the extract, 225 mL of Folin, and 2.5 mL of sodium carbonate 2 % were added, followed by incubation at room temperature for 1 h. Absorbance of the reaction mixture was measured at 750 nm by spectrophotometry (Hitachi, Model 100-20; Hitachi Co., Japan). Quantification of the total phenolic compounds was made from the curve standard of gallic acid (50 to 1250 µg dg.mL-1 - r2 = 0.99, y = 0.0106x).
The same extract with methanol 80 % was used to determine flavonoid compounds. Total flavonoid content was determined by the aluminum chloride colorimetric method (Zacarias et al. 2007Zacarias AA, Moresco HH, Horst H, Brighente IMC, Marques MCA, Pizzollati MG. 2007. Determination of phenolic and flavonoid contents in the extract and fractions of Tabebuia heptaphylla. In: Sociedade Brasileira de Química (ed.) 30th Annual meeting of the Brazilian chemical society. Santa Maria, Sociedade Brasileira de Química. p. 86. ). Briefly, an aliquot of 0.5 mL of extracts was added to 2.5 mL of ethanol and 0.5 mL of 2 % aluminum chloride hexahydrate (AlCl3·6H2O). After incubation at room temperature for 1 h, absorbance of the reaction mixture was measured at 420 nm by spectrophotometry (Hitachi, Model 100-20; Hitachi Co., Japan). The quantification of flavonoids was done from the curve of standard quercetin (700 to 2500 µg.mL-1 - r2 = 0.99, y = 0.0108x).
Light microscopy (LM) and cytochemistry
Thalli samples approximately 5 mm in length were fixed in 2.5 % paraformaldehyde in 0.1 M phosphate buffer (pH 7.2) overnight following the description in Schmidt et al. (2009Schmidt ÉC, Scariot LA, Rover T, et al. 2009. Changes in ultrastructure and histochemistry of two red macroalgae strains of Kappaphycus alvarezii (Rhodophyta, Gigartinales), as a consequence of ultraviolet B radiation exposure. Micron 40: 860-869.). Subsequently, the samples were dehydrated in increasing series of aqueous ethanol solutions and infiltrated with Historesin (Leica Historesin, Heidelberg, Germany). Then, sections 5 µm in length were stained with 0.5 % Toluidine Blue (TB-O), pH 3.0 (Merck Darmstadt, Germany), and used to detect acid polysaccharides through a metachromatic reaction (Gordon & McCandless 1973Gordon EM, McCandless EL. 1973. Ultrastructute and histochemistry of Chondrus crispus Stackhouse. Nova Scotian, Institute of Science.), while Periodic Acid-Schiff (PAS) was used to identify neutral polysaccharides (Gahan 1984Gahan PB. 1984. Plant histochemistry and cytochemistry. New York, Academic Press.). The samples were investigated with an Epifluorescent microscope (Olympus BX 41, Tokyo, Japan) equipped with Image Q Capture Pro 5.1 software (QImaging Corporation, Austin, TX, USA).
Statistical analyses
The data passed the Shapiro-Wilk normality test, and all samples were within normal range. Afterwards, the data were analyzed by unifactorial Analysis of Variance (ANOVA) and Tukey a posteriori test. All samples were compared, using salinity as the independent factor and using a cutoff of p ≤ 0.05. Different letters indicate significant differences according to ANOVA and Tukey’s test (p ≤ 0.05). The statistical analyses were performed using the Statistica software package (Release 10.0).
Results
Morphological features and growth rates
After seven days of exposure to different salinities, A. spicifera presented changes in external morphology. After treatment of 15 psu, the seaweed presented no significant changes, maintaining a greenish brown stipe typical of the species (Fig. 2A). With 20 psu, a depigmentation rendered the stalk yellowish with few branches (Fig. 2B). Treatment with 25 psu led to a slightly depigmented stalk with some new branches (Fig. 2C). Results of the 30 psu treatment were similar to those with 20 psu, leaving a yellowish stalk, but large, new branches (Fig. 2D). After treatments of 35 and 40 psu, the stalks were depigmented with some new branches (Fig. 2E, F). At 45 and 50 psu, no new branches were observed, and the stipe had slight depigmentation (Fig. 2G, H). Thus, at salinities of 20, 25, 30, 35, and 40 psu, external morphological responses were nearly identical, including the presence of new branches and depigmented stipe with a yellowish coloration. On the other hand, in salinities of 15, 45 and 50 psu, the responses showed a slight depigmentation of the stalk and no growth of new branches. All treatments presented a depigmentation of the stipe, but this was already expected because the intensity of PAR in the culture room was lower than that found in the field. The data took this into account, and did not confound the treatment results.
Morphological features of A. spicifera treated with 15, 20, 25, 30, 35, 40, 45 and 50 psu. A, G, H: Note that responses showed a slight depigmentation of the stalk, when compared with the seaweed in the field (Fig. 1), and no growth of new branches. B-F: Observe the presence of new branches (arrows) and depigmented stipe with a yellowish color. Please see the PDF version for color reference.
The treatments were significantly different (Fig. 3), with positive growth rates for treatments of 15 to 35 psu and negative growth rates for 40 to 50 psu. Significantly higher growth rate was observed in the sample exposed to 25 psu, with a growth of 3.60 % per day, while the lowest growth rate, statistically, was found in the sample exposed to 50 psu, with a growth rate of 0.36 % per day.
Growth rates (% day-1) of A. spicifera exposed to 15, 20, 25, 30, 35, 40, 45 and 50 psu for a period of seven days (n = 4; mean ± SD). Different letters indicate significant differences according to unifactorial analysis of variance and Tukey’s test (p ≤ 0.05).
Photosynthetic pigments
Quantification of photosynthetic pigments is summarized in Table 1. After seven days of experimentation, the concentration of chlorophyll a presented significant differences (Tab. 1). The 40 psu sample had the highest concentration of chlorophyll a, with a mean of 73.54 ug/g dry mass, while the 50 psu sample showed the lowest concentration of the pigment, with a mean of 56.07 ug/g dry mass. Samples of 15, 25 and 35 psu were statistically the same as those with higher pigment concentrations, followed by samples of 40 psu with a decrease of 3.28 %, 6.08 % and 4.97 %, respectively, when compared to the 40 psu sample.
Contents of photosynthetic pigments (ug/g dry weight) of chlorophyll a (Chl a), phycobiliproteins (APC: allophycocyanin, PC: phycocyanin, PE: phycoerythrin) and total carotenoids of A. spicifera exposed to 15, 20, 25, 30, 35, 40, 45 and 50 psu for a period of seven days (n = 4; mean ± SD). Different letters indicate significant differences according to bifactorial analysis of variance and Tukey’s test (p ≤ 0.05).
For APC, the highest concentration was observed for the sample treated with 15 psu, and the lowest concentration was observed in the 30 psu sample with 93.84 and 31.50 ug/g dry mass, respectively. The highest concentrations of PC and PE were found in the 20 psu samples with 110.15 and 206.94 ug/g dry mass, respectively. The lowest concentrations were observed in the 30 psu samples with a concentration of 57.54 and 128.01 ug/g dry mass, respectively.
The highest concentration of total carotenoids was observed in the sample treated with 50 psu with a concentration of 379.09 ug/g dry mass. The lowest concentration was observed in the 30 psu sample with 245.38 ug/g dry mass. For all other samples, concentrations of APC, PC, PE, as well as total carotenoids, were close to the highest concentration of the analyzed pigment, which demonstrates a large decline in the concentration of these pigments in the sample treated with 30 psu.
Phenolic and flavonoid compounds
The concentration of total phenolics and flavonoids was significantly affected by salinity (Tab. 2). The highest concentrations of phenolics and flavonoids were observed in the 50 psu sample, with a concentration of 4.57 and 2.01 ug/g of dry weight, respectively. On the other hand, the lowest concentrations of these secondary metabolites were observed in samples treated with 30 psu at 3.07 and 1.35 μg/g of dry weight, respectively.
Total phenolics and flavonoids (ug/g dry weight) of A. spicifera exposed to 15, 20, 25, 30, 35, 40, 45 and 50 psu for a period of seven days (n = 4; mean ± SD). Different letters indicate significant differences according to bifactorial analysis of variance and Tukey’s test (p ≤ 0.05).
LM observations and cytochemistry
Samples of A. spicifera treated with 15, 20, 25, 30, 35, 40, 45 and 50 psu and stained with Toluidine Blue (TB-O) showed a metachromatic reaction in the cell walls (CW), indicating the presence of sulfated acidic polysaccharides, such as sulfated agarans (Fig. 4A-H). The 15 psu sample presented a medium thickening of the cell wall (black arrows) in both cortical (CC) and subcortical (SC) cells, with endophytes (red arrows) (Fig. 4A). Samples treated with 20 to 40 psu also showed a thickening of the cell wall in CCs and SCs (arrows), but no endophytes were present (Fig. 4B-F). However, the samples exposed at 45 and 50 psu presented very thick cell walls when compared to all other samples (Fig. 4G, H). Especially, in the 50 psu sample, a significant disorganization in the format of the cells can be seen, as well as the presence of endophytes (red arrows) (Fig. 4H).
Light microscopy of transversal sections of A. spicifera exposed to 15, 20, 25, 30, 35, 40, 45 and 50 psu for a period of seven days and stained with TB-O. Note the metachromatic reaction of cell wall (CW), cortical cells (CC) and subcortical cells (SC). Note that CC and SC showed a reduction in cell volume and an increase in CW thickness in all salinities (black arrows), while greater thickening was observed in the 45 and 50 psu samples. Note the presence of endophytes (red arrows) in 15 and 50 psu samples. Please see the PDF version for color reference.
Samples stained with Periodic Acid-Schiff (PAS) showed a reaction with the cellulose compounds present in the cell wall, and in the cytoplasm, a positive reaction to starch grains was observed (Fig. 5A-H ). In samples of 15, 20 and 25 psu, a large amount of starch grains (Fig. 5A-C) was observed. On the other hand, in the 30 psu sample, the amount of starch grains was very small (Fig. 5D), whereas the 35, 40, 45 and 50 psu samples presented a larger amount of starch grains (Fig. 5E-H) when compared to the 30 psu sample, but a smaller amount when compared to the 15, 20 and 25 psu samples.
Light microscopy of transversal sections of A. spicifera exposed to 15, 20, 25, 30, 35, 40, 45 and 50 psu for a period of 7 days and stained with PAS. Note the reaction with the cellulose compounds present in the cell wall (CW) and the presence the starch grains (S). Note the large amount of starch observed in 15, 20 and 25 psu samples. In contrast, the amount of starch grains in the 30 psu sample was very small, while the 35, 40, 45 and 50 psu samples presented a larger amount of starch grains when compared to the sample treated with 30 psu.
Discussion
Samples of the red alga A. spicifera exposed to 15, 20, 25, 30, 35, 40, 45 and 50 psu showed important physiological responses. Since the intensity of PAR in the culture room was lower than that found in the field, all treatments presented a depigmentation of the stipe. According to Fenchel & Straarup (1971Fenchel T, Straarup BJ. 1971. Vertical distribution of photosynthetic pigments and the penetration of light in marine sediments. Oikos 22: 172-182. ), with greater depth of water, less ingress of light can be expected, resulting in lower concentrations of chlorophyll a and phycocyanin in the sediment. Thus, since light intensity is reduced, algae can conserve energy by producing fewer pigment molecules. In the present study with less available light, pigments were diminished in specimens cultivated under 15, 45 and 50 psu, and these were the same specimens that presented most difference relative to external morphology, i.e., absence of new stalks. The tetrasporangia of A. spicifera occur at the base of short branches, leaving the stipe a little lighter in color compared to that in the infertile region (Cordeiro-Marino 1978Cordeiro-Marino M. 1978. Rodentíceas marine bentônicas of the State of Santa Catarina. Instituto de Botânica 7: 1-243.). In treatments of 20 to 40 psu, a depigmentation was perceived in the center of the stalk, but with growth of new branches. In addition to PAR intensity, this depigmentation may indicate that the algae are trying to propagate the species in the form of spore release, showing that the reproductive cycle could be maintained normally in these salinities. In addition, the growth rate was negative in samples treated with 40, 45 and 50 psu, but positive in the 15 psu sample which, however, did not present new branches. This result may have resulted from cell wall thickening in this treatment, as seen in the TB-O in LM, which can lead to weight gain. Fluctuation in salinity leads to cellular stress in algae, in turn causing membrane leakage of ions and electrolytes, pH changes, solute crystallization and pro tein denaturation (Collén & Davison 1999Collén J, Davison IR. 1999. Reactive oxygen metabolism in intertidal Fucus spp. (Phaeophyceae). Journal of Phycology 35: 62-69.; Bischof & Rautenberger 2012Bischof K, Rautenberger R. 2012. Seaweed responses to environmental stress: reactive oxygen and antioxidative strategies. In: Wiencke C, Bischof K. (eds.) Seaweed biology. Berlin, Heidelberg, Springer. p. 109-132.; Karsten & Holzinger 2012Karsten U, Holzinger A. 2012. Light, temperature, and desiccation effects on photosynthetic activity, and drought-induced ultrastructural changes in the green alga Klebsormidium dissectum (Streptophyta) from a high alpine soil crust. Microbial Ecology 63: 51-63.; Kumar et al. 2012Kumar M, Bijo AJ, Baghel RS, Reddy CRK, Jha B. 2012. Selenium and spermine alleviate cadmium induced toxicity in the red seaweed Gracilaria dura by regulating antioxidants and DNA methylation. Plant Physiology and Biochemistry 51: 129-138.). These events then cause changes in many different physiological processes with the corresponding accumulation of reactive oxygen species (ROS) (Collén & Davison 1999Collén J, Davison IR. 1999. Reactive oxygen metabolism in intertidal Fucus spp. (Phaeophyceae). Journal of Phycology 35: 62-69.; Bischof & Rautenberger 2012Bischof K, Rautenberger R. 2012. Seaweed responses to environmental stress: reactive oxygen and antioxidative strategies. In: Wiencke C, Bischof K. (eds.) Seaweed biology. Berlin, Heidelberg, Springer. p. 109-132.; Karsten & Holzinger 2012Karsten U, Holzinger A. 2012. Light, temperature, and desiccation effects on photosynthetic activity, and drought-induced ultrastructural changes in the green alga Klebsormidium dissectum (Streptophyta) from a high alpine soil crust. Microbial Ecology 63: 51-63.; Kumar et al. 2012Kumar M, Bijo AJ, Baghel RS, Reddy CRK, Jha B. 2012. Selenium and spermine alleviate cadmium induced toxicity in the red seaweed Gracilaria dura by regulating antioxidants and DNA methylation. Plant Physiology and Biochemistry 51: 129-138.). It is also well known that hypersaline stress is the most harmful to algae since it involves water deprivation. This is different from hyposaline stress where osmotic shock promotes cellular hydration (Kumar et al. 2014Kumar M, Kumari P, Reddy CRK, Jha B. 2014. Salinity and desiccation induced oxidative stress acclimation in seaweeds. London, Elsevier.). With turgor pressure reduced by high salinity, cell division is halted. This can explain the negative growth rate at the higher salinities (40, 45 and 50 psu). This was also verified in Hypnea cervicornis (Ding et al. 2013Ding L, Ma Y, Huang B, Chen S. 2013 Effects of seawater salinity and temperature on growth and pigment contents in Hypnea cervicornis J. Agardh (Gigartinales, Rhodophyta). Biomed Research International 2013: 1-10. ) and S. alsidii (Nitschke et al. 2014Nitschke U, Karsten U, Eggert A. 2014. Journal of experimental marine biology and ecology physiological performance of the red alga Stylonema alsidii (Stylonematophyceae) under varying salinities. Journal of Experimental Marine Biology and Ecology 460: 170-176. ).
Chlorophyll a is the main photosensitizing pigment of red algae (Franceschini et al. 2009Franceschini IM, Burliga AL, Reviers B, Prado JF, Hamlaoui S. 2009. Algae: a phylogenetic, taxonomic and ecological approach. Porto Alegre, Artmed Editora.; Suggett & Prášil 2010Suggett DJ, Prášil O. 2010. Chlorophyll a fluorescence in aquatic sciences: methods and applications. Dordrecht, Springer.), and the lowest concentration of this pigment was observed in the 50 psu sample. With little chlorophyll, the photosynthetic rate tends to decrease, explaining, in turn, the low growth rate in this sample. This decrease in chlorophyll content was most likely associated with the degenerative process of pigment biosynthesis as a compensation mechanism to maintain the availability of carbon compounds for the synthesis of antioxidant compounds, as observed in Palmaria palmata (Holzinger & Lütz 2006Holzinger A, Lütz C. 2006. Algae and UV irradiation: Effects on ultrastructure and related metabolic functions. Micron 37: 190-207. ) and Phycodrys austrogeorgica (Poppe et al. 2003Poppe F, Schmidt R, Hanelt D, Wiencke C. 2003. Effects of UV radiation on the ultrastructure of several red algae. Phycological 51: 11-19. ) stressed by ultraviolet radiation. At high salinities, the chlorophyll concentration was also low in K. alvarezii (Araújo et al. 2014Araújo PG, Ribeiro ALNL, Yokoya NS, Fujii MT. 2014. Temperature and salinity responses of drifting specimens of Kappaphycus alvarezii (Gigartinales, Rhodophyta) farmed on the Brazilian tropical coast. Journal of Applied Phycology 26: 1979-1988. ).
Allophycocyanin, phycocyanin and phycoerythrin have three main functions in red algae. They first serve as accessory photosynthetic pigments capturing different wavelengths for the realization of photosynthesis (Suggett & Prášil 2010Suggett DJ, Prášil O. 2010. Chlorophyll a fluorescence in aquatic sciences: methods and applications. Dordrecht, Springer.). Next, they promote photoprotection. To accomplish this, phycobiliproteins are organized in a complex antenna in the thylakoid membrane where APC occupies the center above chlorophyll, followed by PC and PE at the terminus (Parmar et al. 2013Parmar A, Singh NK, Dhoke R, Madamwar D. 2013. Influence of light on phycobiliprotein production in three marine cyanobacterial cultures. Acta Physiologiae Plantarum 35: 1817-26.). This arrangement assists in the process of photoprotection of chlorophyll a by preventing the intensity of light and radiation from reaching this important pigment (Watanabe & Ikeuchi 2013Watanabe M, Ikeuchi M. 2013. Phycobilisome: Architecture of a light-harvesting supercomplex. Photosynthesis Research 116: 265-76.; Kaňa et al. 2014Kaňa R, Kotabová E, Lukeš M, et al. 2014. Phycobilisome mobility and its role in the regulation of light harvesting in red algae. Plant Physiology 165: 1618-31.). Finally, APC, PC and PE serve as antioxidants to prevent oxidative stress (Cano-Europe et al. 2010Cano-Europe E, Ortiz-Butrón R, Gallardo-Casas CA, et al. 2010. Phycobiliproteins from Pseudanabaena tenuis rich in c-phycoerythrin protect against HgCl2-caused oxidative stress and cellular damage in the kidney. Journal of Applied Phycology 22: 495-501.; Kumar et al. 2010Kumar M, Kumari P, Gupta V, et al. 2010. Biochemical responses of red alga Gracilaria corticata (Gracilariales, Rhodophyta) to salinity induced oxidative stress Journal of Experimental Marine Biology and Ecology 391: 27-34.). The lowest concentrations of these three accessory pigments were verified in the 30 psu treatment (Tab. 1). Because it is an ideal salinity, the seaweed does not suffer from stress, thereby reaching a homeostasis that allows the algae to achieve a photosynthesis rate with its chlorophyll, while, at the same time, reducing the need for large amounts of pigments to aid in the capture of light energy. Plants produce ROS continuously as by-products of aerobic metabolism, but when they are under some stress, the amount of ROS tends to increase. Therefore, the plant needs to recruit its antioxidant agents to avert possible oxidative damage (Apel & Hirt 2004Apel K, Hirt H. 2004. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology 55: 373-399. ; Takahashi & Badger 2011Takahashi S, Badger MR. 2011. Photoprotection in plants: a new light on photosystem II damage. Trends in Plant Science 16: 53-60.). Since the seaweed at 30 psu represents its normal, homeostatic state, it produces little ROS and does not require the production of antioxidant agents, such as phycobiliproteins. On the other hand, the largest concentrations of phycobiliproteins were observed in the samples with lower salinities (15 and 20 psu), demonstrating that these treatments do, indeed, cause stress by the increased requirement for light and antioxidants (phycobiliproteins, carotenoids, phenols and flavonoids) when compared with the 30 psu treatment (Tabs. 1, 2), even though the algae still retain the capacity to protect this mechanism. This increase in the concentrations of phycobiliproteins under stress was also verified in Aglaothamnion uruguayense under ultraviolet radiation (Ouriques et al. 2017Ouriques LC, Pereira DT, Simioni C, et al. 2017. Physiological, morphological and ultrastructural responses to exposure to ultraviolet radiation in the red alga Aglaothamnion uruguayense (WR Taylor). Brazilian Journal of Botany 1-9.). However, UVR stress is different from that under high salinities that place seaweeds under even greater stress, which then forces the reduction of this defense pathway in order to channel energy toward more important defense mechanisms, such as the production of carotenoids, phenols and flavonoids.
Carotenoids, phenols and flavonoids are part of the secondary metabolism of red algae and serve as antioxidants (Zeng et al. 2001Zeng L-M, Wang C-J, Su J-Y, et al. 2001. Flavonoids from the red alga Acanthophora spicifera. Chinese Journal of Chemistry 19: 1097-1100.; Apel & Hirt 2004Apel K, Hirt H. 2004. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology 55: 373-399. ; Ganesan et al. 2008Ganesan P, Kumar CS, Bhaskar N. 2008. Antioxidant properties of methanol extract and its solvent fractions obtained from selected Indian red seaweeds. Bioresource Technology 99: 2717-2723. ; Kottuparambil et al. 2012Kottuparambil S, Shin W, Brown MT, Han T. 2012. UV-B affects photosynthesis, ROS production and motility of the freshwater flagellate, Euglena agilis Carter. Aquatic Toxicology 122: 206-213. ). All three metabolites were in higher concentrations in the samples treated with 50 psu, but lower concentrations in those treated with 30 psu, the latter repeating the pattern and motif found with phycobiliproteins. As previously seen, hypersalinity stress is much stronger when compared hyposalinity; therefore, the former causes greater cellular disorganization requiring the deployment of antioxidants. The same results were found in Dunaliella salina (Chlorophyta) (Marin et al. 1998Marín N, Morales F, Lodeiros C, Tamigneaux E. 1998. Effect of nitrate concentration on growth and pigment synthesis of Dunaliella salina cultivated under low illumination and preadapted to different salinities. Journal of Applied Phycology 10: 405-411.), Scenedesmus sp. (Chlorophyta) (Aburai et al. 2015Aburai N, Sumida D, Abe K. 2015. Effect of light level and salinity on the composition and accumulation of free and ester-type carotenoids in the aerial microalga Scenedesmus sp. (Chlorophyceae). Algal Research 8: 30-36. ), and P. capillacea (Schmidt et al. 2015Schmidt ÉC, Marthiellen MR, Polo LK, et al. 2015. Influence of cadmium and salinity in the red alga Pterocladiella capillacea: cell morphology, photosynthetic performance and antioxidant systems. Brazilian Journal of Botany 38: 737-749. ).
Upon hypersaline stress, a thickening of the cell wall was also confirmed to restrict the loss of water to the external environment. The sulfated agarans found in the cell wall of A. spicifera is a hydrophilic polysaccharide (Vasconcelos et al. 2015Vasconcelos AG, Araújo KV, Santana LAB. 2015. Polysaccharides extracted from marine algae and their biotechnological applications: a review. Revista Brasileira de Inovação Tecnológica em Saúde 5: 27-51.); thus, cell wall thickening also attracts and stores water. In addition, cells in 50 psu samples were weakened, favoring the presence of endophytes in the cell wall. Endophytes were also observed in samples treated with 15 psu, indicating that this salinity leaves the cell in a fragile condition. The composition of the host cell wall seems to determine the degree of endophyte infectivity, and infection is more common when a host cell is weak (Correa & McLachlan 1991Correa JA, McLachlan JL. 1991. Endophytic algae of Chondrus crispus (Rhodophyta). III. Host specificity. Journal of Phycology 27: 448-459. ). Although the causal mechanism of endophyte penetration is unknown in the tetrasporophyte, its presence did indicate some change, perhaps a loss of defenses, in these algae at 15 and 50 psu.
Red algal starch is known as "florid starch", and it is an extraplastidial starch product (Reviers 2006Reviers, B. 2006. Biology and phylogeny of algae. 2nd edn. Porto Alegre, Artmed.; Franceschini et al. 2009Franceschini IM, Burliga AL, Reviers B, Prado JF, Hamlaoui S. 2009. Algae: a phylogenetic, taxonomic and ecological approach. Porto Alegre, Artmed Editora.). The lowest amount of starch was verified in the 30 psu sample, while samples at all other salinities had a large amount of this material. Siaut et al. (2011 Siaut M, Cuiné S, Cagnon C, et al. 2011. Oil accumulation in the model green alga Chlamydomonas reinhardtii: characterization, variability between common laboratory strains and relationship with starch reserves. Biotechnology 11: 7.) showed that carbohydrate accumulation in Chlamydomonas reinhardtii (Chlorophyta) increased in response to immediate saline shock, corroborating the results of the present work.
Therefore, it can be concluded that the red seaweed A. spicifera has high tolerance to salinities of 25, 30, 35 and 40 psu, with little change in morphology and physiology, thus favoring the expansion of this species in diverse environments. However, salinities of 15, 20, 45 and 50 psu were the most damaging and led to loss of biomass and depigmentation of apices. The 50 psu treatment caused the greatest changes overall, greatly reducing biomass and chlorophyll and facilitating the presence of endophytes, thus indicating that high, more than low, salinity interferes with algae metabolism. In sum, for environments which show higher salinity, the results from this study show that this species could have limited distribution in the future, or become seriously threatened, which would, in turn, have pronounced ecological consequences.
Acknowledgements
The authors would like to acknowledge the staff of the Multiuser Laboratory of Biology Studies (LAMEB), Federal University of Santa Catarina, Florianópolis, SC, Brazil, for the use of their light microscopy. The authors acknowledge the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil) for the financial support. Zenilda L. Bouzon is a CNPq fellow.
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Publication Dates
-
Publication in this collection
21 Sept 2017 -
Date of issue
Oct-Dec 2017
History
-
Received
15 Feb 2017 -
Accepted
04 May 2017