Equine platelet-rich plasma cryopreservation

P. Fantini M.S. Palhares M. Prades V.C. Macedo J.M. Silva Filho F.O.P. Leme J.U. Carmona About the authors

Equine platelet-rich plasma (PRP) frozen at -196°C in liquid nitrogen using DMSO as a cryoprotectant in two different concentrations (3% and 6%) was evaluated, using platelet morphology and aggregometry as the final parameters. Twelve PRP samples were used in two repetitions. The samples were submitted to slow cooling prior to frozen (-0.07°C/minute) until they reached the temperature of 4-5°C. Platelet cryopreserved in 3% or 6% DMSO, presented similar efficacy when the percentage of activation and platelet aggregation was evaluated.

Equine; platelet-rich plasma (PRP); cryopreservation; DMSO

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