Taenia solium metacestode antigens in ELISA for the diagnosis of bovine cysticercosis

L.L. Monteiro P.S.A. Pinto J.H.P. Salcedo J.V. Araújo W.L.M. Santos P.R. Cecon About the authors

Some parameters of ELISA were evaluated using positive and negative bovine sera for cysticercosis and three types of antigens of Taenia solium larvae: total, scolex and membrane. The antigen concentrations (0.25; 0.5; 1; 2 and 4µg/well) and the serum dilutions (1:25, 1:50, 1:100 and 1:200) were the parameters that influenced less the test performance; while blocking substance, skimmed milk, and conjugate dilutions, 1:1.250, 1:2.500 and 1:5.000 were the best indexes of the test performance. It was concluded that this combination of criteria should be considered in the diagnosis of bovine cysticercosis, in routine diagnosis and for the ELISA test standardization.

bovine cysticercosis; ELISA; antigen; Taenia solium

Universidade Federal de Minas Gerais, Escola de Veterinária Caixa Postal 567, 30123-970 Belo Horizonte MG - Brazil, Tel.: (55 31) 3409-2041, Tel.: (55 31) 3409-2042 - Belo Horizonte - MG - Brazil
E-mail: abmvz.artigo@abmvz.org.br