Protection for glucan in experimental model of sepsis

PURPOSE: This study evaluated the insoluble (1 ® 3) b -glucan action, a polysaccharide isolated from the inner cell wall of the yeast Saccharomyces cerevisiae, as nonspecific immunostimulant agent in mice underwent experimental model of sepsis. METHODS: Seventy three Swiss mices received different doses of glucan by intraperitoneal (i.p.) administrations. Difuse sepsis was induced by the cecal puncture and ligation. RESULTS: The glucan-treated group showed a significant increase in the leukocyte number at the peritoneal fluid, and decrease in the bacterial colony-forming units. It was observed an increase in the survival rate at the insoluble glucan-treated group, when compared to controls. CONCLUSION: The insoluble glucan, when used in mices with peritoneal sepsis, enhances survival, induces protection against bacterial colonization of peritoneal fluid and increasing in leukocite migration.

Glucan; Sepsis; Survival; Leukocite migration; Bacterial colony

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