Schinus terebinthifolius raddi (Aroeira) and Orbignya phalerata mart. (Babassu) effect in cecorrahphy healing in rats

Scheibe, Christian Lamar; Ribas-Filho, Jurandir Marcondes; Czeczko, Nicolau Gregori; Malafaia, Osvaldo; Barboza, Luiz Eduardo Durães; Ribas, Fernanda Marcondes; Wendler, Eduardo; Torres, Orlando; Lovato, Fernanda Christo; Scapini, João Guilherme Seifert

doi:10.1590/S0102-865020160060000007

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Original Articles • Acta Cir. Bras. 31 (06) • June 2016 • https://doi.org/10.1590/S0102-865020160060000007 copy

Schinus terebinthifolius raddi (Aroeira) and Orbignya phalerata mart. (Babassu) effect in cecorrahphy healing in rats^¹ 1 Research performed at Postgraduate Program in Principles of Surgery, Faculdade Evangélica do Paraná (FEPAR), Universidade Evangélica do Paraná, Hospital de Curitiba, Medical Research Institute, Curitiba-PR, and Hospital São Domingos, Sao Luis-MA, Brazil. Part of Master degree thesis, Postgraduate Program in Principles of Surgery. Tutor: Jurandir Marcondes Ribas Filho.

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

PURPOSE:

To evaluate the effect of Schinus terebinthifolius Raddi (aroeira) and Orbignya phalerata Mart. (babassu) in the healing process of cecorrhaphy in rats.

METHODS

: Fifty four rats were used, distributed into three groups randomly: aroeira, babassu and control, which were divided into three subgroups (six animals) according to the time of the deaths (7, 14, 21 days). All underwent the same surgical procedure, cecotomy and cecorrhaphy. The animals in group aroeira and babassu received daily dose of 100 mg/kg of hydroalcoholic extract and 50 mg/kg of aquous extract respectively, by gavage. The control group received only saline solution. The parameters evaluated were: macroscopic changes, ,resistance test to air insufflations and histological changes.

RESULTS

: All animals showed good healing without infection. All groups presented adhesions between cecum and neighboring organs. The resistance test insufflating of atmospheric air showed progressive increase of pressure according to the days in the aroeira group, and decrease in babassu group, without significant difference. Microscopy showed significant difference in the polymorphonuclear, hyperemia, angiogenesis, fibroblast proliferation and collagen histological variables in the 14^th day.

CONCLUSION

: Hydroalcoholic extract of aroeira and the aqueous extract of babassu favored the healing process in cecorrhaphy in rats.

Key words:
Wound Healing; Colon; Phytotherapy; Plant Extracts; Rats.

Day	Nair³⁷	Group			p
Day	Nair³⁷	Control	Aroeira	Babassu
7	0	0 (0.0)	1 (16.7)	0 (0.0)	0.638
	I	3 (50.0)	3 (50.0)	3 (50.0)
	II	3 (50.0)	2 (33.3)	2 (33.3)
	III	0 (0.0)	0 (0.0)	1 (16.7)
14	I	1 (16.7)	2 (33.3)	2 (33.3)	0.126
	II	2 (33.3)	4 (66.7)	4 (66.7)
	III	3 (50.0)	0 (0.0)	0 (0.0)
21	0	1 (16.7)	1 (16.7)	0 (0.0)	0.014
	I	4 (66.7)	0 (0.0)	0 (0.0)
	II	1 (16.7)	5 (83.3)	6 (100.0)
In general	0	1 (5.6)	2 (11.1)	0 (0.0)	0.195
	I	8 (44.4)	5 (27.8)	5 (27.8)
	II	6 ( 33.3)	11 (61.1)	12 (66.7)
III	3 (16.7)	0 (0.0)	1 (5.6)

Days	Group	Rupture site		p
Days	Group	Out of the suture	On the suture
Control	7	1	5	0.135
	14	4	2
	21	4	2
Aroeira	7	4	2	0.049
	14	2	4
	21	6	0
Babassu	7	4	2	1.000
	14	4	2
	21	4	2

Day			Group		p
Day		Control	Aroeira	Babassu	p
7	Polymorphonuclear	Mod./Accent.	Mod./ Accent.	Accent.	0.385
	Monomorphonuclear	Mod./Accent.	Accent.	Mod.	0.057
	Edema	Mod.	Mod.	Mod.	0.322
	Congestion	Mod./Accent.	Accent.	Accent.	0.150
	Angiogenesis Fibroblast proliferation	Mod. Mod./Accent.	Accent. Accent.	Mod./Accent. Accent.	0.533 0.123
	Fibrosis	Slight	Slight	Abs	0.189
	Collagen	Slight	Slight	Abs/ Slight	0.284
14	Polymorphonuclear	Mod. a	Slight/Mod. ab	Abs b	0.004
	Monomorphonuclear	Accent.	Mod./ Accent.	Slight/Mod.	0.104
	Edema	Mod.	Slight/Mod.	Slight/Mod.	0.809
	Congestion	Accent. a	Mod. b	Slight c	0.004
	Angiogenesis Fibroblast proliferation	Mod. a Accent. a	Mod./ Accent. a Accent. a	Slight b Mod. b	0.011 0.030
	Fibrosis	Slight	Slight	Abs	0.056
	Collagen	Slight a	Slight a	Abs b	0.033
21	Polymorphonuclear	Abs/Slight	Slight	Slight	0.414
	Monomorphonuclear	Mod.	Mod.	Mod.	0.087
	Edema	Slight	Slight/Mod.	Mod.	0.533
	Congestion	Mod.	Mod.	Mod.	0.812
	Angiogenesis Fibroblast proliferation	Slight/Mod. Accent.	Slight/Mod. Accent.	Slight Accent.	0.757 0.298
	Fibrosis	Abs/ Slight	Abs	Abs	0.492
	Collagen	Abs/ Slight	Slight	Abs	0.533

	Day/ Group	GC	GA	GB	p
Polymorphonuclear	7	Mod./Accent.	Mod./Accent.	Accent.	0.385
	14	Mod. a	Slight/Mod. ab	Abs b	0.004*
	21	Abs/Slight	Slight	Slight	0.414
Edema	7	Mod.	Mod.	Mod.	0.322
	14	Mod.	Slight/Mod.	Slight/Mod.	0.809
	21	Slight	Slight/Mod.	Mod.	0.533
Congestion	7	Mod./Accent.	Accent.	Accent.	0.150
	14	Accent. a	Mod. b	Slight c	0.004
	21	Mod.	Mod.	Mod.	0.812
Monomorphonuclear	7	Mod./ Accent.	Accent.	Mod.	0.057
	14	Accent.	Mod./ Accent.	Slight/Mod.	0.104
	21	Mod.	Mod.	Mod.	0.087
Angiogenesis	7	Mod.	Accent.	Mod./Accent.	0.533
	14	Mod. a	Mod./ Accent. a	Slight b	0.011
	21	Slight/Mod.	Slight/Mod.	Slight	0.757
Fibroblast proliferation	7	Mod./Accent.	Accent.	Accent.	0.123
	14	Accent. a	Accent. a	Mod. b	0.030
	21	Accent.	Accent.	Accent.	0.298
Fibrosis	7	Slight	Slight	Abs	0.189
	14	Slight	Slight	Abs	0.056
	21	Abs/Slight	Abs	Abs	0.492
Collagen	7	Slight	Slight	Abs/Slight	0.284
	14	Slight a	Slight a	Abs b	0.033
	21	Abs/Slight	Slight	Abs	0.533

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[1] Correspondence: Christian Lamar Scheibe Alameda Augusto Stellfeld, 1980 80730-150 Curitiba - PR Brasil Tel.: (55 41)3240-5488 clscheibe@uol.com.br