PURPOSE: To study for clinical and morphological point of views, the effects of a chalcone in allergic conjunctivitis induced by ovalbumim in guinea pig. METHODS: Fifty-four white male guinea pigs weighing approximately 400 g were used in the study. All animals were sensitized by intraperitoneal injection of ovalbumin suspended in Freund’s complete adjuvant solution. Conjunctivitis was induced by the ovalbumin by instillation of ovalbumin in conjunctival sac of right eye´s. The animals were randomized in 3 groups agreement with the proposed treatment: chalcone,corticoid and saline. The clinical analysis were recorded at 5, 10 e 40 min, 7 e 24 h after induced and daily until 7 days after induced. Morphologic analysis consisted in evaluating edema, necrosis, vascularization and exocytosis at the 1, 3 and 7 days following induction of conjunctivitis. RESULTS: Clinical alterations were more accentuated 24 h after induction. Inflammatory signs were less apparent in chalcone group as compared to saline Corticoid group presented less inflammatory signs when compared to chalcone and control groups. Morphologic analysis disclosed that chalcona effect is similar to corticoid effects. CONCLUSION: It is concluded that chalcona had therapeutic effect in the allergic conjunctivitis induced by ovalbumine in guinea pigs.
Allergy conjunctivitis; Ovalbumin; Chalcone; Guinea pig
