Cinética da absorção de cálcio na presença de magnésio em células foliares de cafeeiro (Coffea arabica L. cv. 'Mundo Novo')

Sarruge, José R.; Biasi, José; Castro, Paulo R. C.; Defelipo, Braz V.; Feitosa, Celi; Krug, Francisco J.; Morelli, Maurelio

doi:10.1590/S0071-12761973000100033

Resumos

No trabalho apresentado, estudou-se a cinética de absorção de íons, baseado na equação de LINEWEAVER & bURk (1934). o estudo visou verificar o efeito competitivo ou não do íon Mg²+ na absorção do íon Ca²+. Para facilidade do estudo, soluções de CaCl2 marcadas com 45Ca foram administradas em diferentes concentrações à tecidos foliares de café (Coffea arabica L., cv. 'Mundo Novo'), em solução contendo ou não uma concentração conhecida de MgCl2 segundo a técnica de SMITH & EPSTEIN (1964a e 1964b). São apresentados também cálculos estatísticos, considerados inéditos na aplicação do presente estudo. Conclue-se que há inibição competitiva devida ao íon Mg²+ pelos centros ativos dos carregadores.

This study based on LINEWEAVER & BURK's (1934) equation was made to verify both competitive or not effect of Mg2_f in absorption of Ca2+ ion. Solution of CaCl2 was traced with 45Ca. Calcium in different concentration was added to excised leaf tissue of Coffee-tree (Coffea arabica L.) in presence or not of magnesium, according to SMITH & EPSTEIN's (1964a.; 1964b.) technique. Special conduction in statistics was applied. Magnesium ion compete with calcium ion to active centers of metabolism transport.

José R. Sarruge^I; José Biasi^II; Paulo R. C. Castro^III; Braz V. Defelipo^IV; Celi Feitosa^V; Francisco J. Krug^VI; Maurelio Morelli^VII

^IDepartamento de Química, E. S. A. "Luiz de Queiroz" U. S. P

^IIEstação Experimental de Rio Caçador, EMBRAPA bolsista CNPq

^IIIDepartamento de Botânica, E. S. A. "Luiz de Queiroz" U. S. P

^IVDepartamento de Fitotecnia, E. S. A. U. F. Viçosa bolsista CAPES

^VSeção de Fertilidade do Solo, i. A. E. S. P. bolsista CNPq

^VIBolsista CNEN CENA

^VIIDepartamento de Solos, F. A. U. F. Santa Maria

RESUMO

No trabalho apresentado, estudou-se a cinética de absorção de íons, baseado na equação de LINEWEAVER & bURk (1934). o estudo visou verificar o efeito competitivo ou não do íon Mg²+ na absorção do íon Ca²+. Para facilidade do estudo, soluções de CaCl₂marcadas com ⁴⁵Ca foram administradas em diferentes concentrações à tecidos foliares de café (Coffea arabica L., cv. 'Mundo Novo'), em solução contendo ou não uma concentração conhecida de MgCl₂ segundo a técnica de SMITH & EPSTEIN (1964a e 1964b). São apresentados também cálculos estatísticos, considerados inéditos na aplicação do presente estudo.

Conclue-se que há inibição competitiva devida ao íon Mg²+ pelos centros ativos dos carregadores.

SUMMARY

This study based on LINEWEAVER & BURK's (1934) equation was made to verify both competitive or not effect of Mg^2_f in absorption of Ca²⁺ ion. Solution of CaCl₂ was traced with ⁴⁵Ca. Calcium in different concentration was added to excised leaf tissue of Coffee-tree (Coffea arabica L.) in presence or not of magnesium, according to SMITH & EPSTEIN's (1964a.; 1964b.) technique. Special conduction in statistics was applied.

Magnesium ion compete with calcium ion to active centers of metabolism transport.

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LITERATURA CITADA

Entregue para publicação em 28/12/1973.

BRAY, H. G. & WHITE, K., 1966 Kinetics and thermodinamics in biochemistry. 2.Ş edição. J. A. CHURCHILL Ltd., London. 418 pp.
BOWEN, H. J. M., 1966 Trace elements in Biochemistry. Academic Press. Inc., London. 241 pp.
COLLANDER, R., 1944 Selective adsorption of cation by higher plants. Plant Physiol. 16: 691-720.
CRUZ, A. D., 1973 Absorção de cálcio pelos tecidos da folha do cafeeiro (Coffea arabica L., var. Mundo Novo). Tese para obtenção do títudo de doutor, ESALQ Piracicaba. 43 pp.
EPSTEIN, E., 1953 Mechanism of ion absorption by roots. Nature. 171: 83-84.
EPSTEIN, E., 1956 Mineral nutrition of plants: Mechanisms of uptake and transport. Ann. Rev. Plant Physiol. 7: 1-24.
EPSTEIN, E., 1961 The essencial role of calcium in selective cation transport by plant cells Plant. Physiol. 31: 437-444.
EPSTEIN, E., 1962 Mutual effects of ions in their absorption by plants. Agrochimica. 6: 293-322.
EPSTEIN, E., 1972 Mineral nutrition of plants: Principles and perspectives. John Wiley & Sons. New York.
EPSTEIN, E. & HAGEN, C. E., 1952 A kinetic study of the absorption of alkali cations by barley roots. Plant Physiol. 27: 457-474.
EPSTEIN, S. & LEGGETT, I. E. 1954 The absorption of alkaline earth cations by barley roots: Kinetics and mechanism. Amer. J. Bot. 41(10): 785-791
FRIED, M. & BROESCHART, 1972 The soil-plant system in relation to inorganic nutrition. Academic Press, London. 358 pp.
FRIED, M. & SHAPIRO, R. E., 1961 Soil-plant relationships in ion uptake. Ann. Rev. Plant Physiol. 12: 91-112.
HOAGLAND, D. R., 1944 Lectures of the inorganic nutrition of plants. In Inorganic Plant Nutrition. Published by Chronica Botânica Comp., Waltham, Massassuchets. 226 pp.
JACKSON, P. C., HENDRICKS, S. B. & VASTA, B. M., 1962 Phosphorylation by barley roots mitochondria and phosphate absorption by barley roots. Plant Physiol. 37: 8-17
LINEWEAVER, H. & BURK, D., 1934 The determination of enzyme dissociation constants. J. Amer. Chem. Soc. 56: 658-666.
LUNDEGARDH, H., 1939 An eletro-chemical theory of salt absorption and respiration. Nature. 143: 203-206.
MAAS, E. V., MOORE,. D. P. & MASON, B. J., 1969 Influence of calcium and magnesium oh manganese absorption. Plant Physiol. 44: 796-800.
MICHAELIS, L. & MENTEN, M. L., 1913 Die Kinetic der Invertinwirkung. Biochem. Z. 49: 333-369.
MITCHELL, P., 1961 Coupling of phosphorylation to elétron and hidrogen transfer by a chemiosmotic type of mechanism. Nature. 191: 144-148.
MITCHELL, P., 1966 Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Biol. Rev. 41: 445-502.
MOORE, D. P., JACOBSON, L. & OVERSTREET, R., 1961 Uptake of calcium by excised barley roots. Plant Physiol. 36: 53-57.
SALISBURY, F. B. & ROSS, C., 1969 Plant physiology. Wadsworth Publishing Comp. Belmont, California. 747 pp.
SMITH, R. C. & EPSTEIN, E., 1964a Ion absorpition by shoot tissue: Technique and first findings with excised leaf tissue of corn. Plant Physiol. 39: (3) : 338-341.
SMITH, R. C. & EPSTEIN, E., 1964b Ion absorption by shoot tissue: Kinetics of potassium and rubidium absorption by corn leaf tissue. Plant Physiol. 39 (6) : 992-996.

**Cinética da absorção de cálcio na presença de magnésio em células foliares de cafeeiro (Coffea arabica L. cv. 'Mundo Novo')**

Kinetic of calcium absorption in presence of magnesium in excised leaf tissue of coffee-tree (Coffea arabica L., cv 'Mundo Novo')

Datas de Publicação

Publicação nesta coleção
29 Maio 2012
Data do Fascículo
1973

Histórico

Recebido
28 Dez 1973

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] BRAY, H. G. & WHITE, K., 1966 Kinetics and thermodinamics in biochemistry. 2.Ş edição. J. A. CHURCHILL Ltd., London. 418 pp.

[2] BOWEN, H. J. M., 1966 Trace elements in Biochemistry. Academic Press. Inc., London. 241 pp.

[3] COLLANDER, R., 1944 Selective adsorption of cation by higher plants. Plant Physiol. 16: 691-720.

[4] CRUZ, A. D., 1973 Absorção de cálcio pelos tecidos da folha do cafeeiro (Coffea arabica L., var. Mundo Novo). Tese para obtenção do títudo de doutor, ESALQ Piracicaba. 43 pp.

[5] EPSTEIN, E., 1953 Mechanism of ion absorption by roots. Nature. 171: 83-84.

[6] EPSTEIN, E., 1956 Mineral nutrition of plants: Mechanisms of uptake and transport. Ann. Rev. Plant Physiol. 7: 1-24.

[7] EPSTEIN, E., 1961 The essencial role of calcium in selective cation transport by plant cells Plant. Physiol. 31: 437-444.

[8] EPSTEIN, E., 1962 Mutual effects of ions in their absorption by plants. Agrochimica. 6: 293-322.

[9] EPSTEIN, E., 1972 Mineral nutrition of plants: Principles and perspectives. John Wiley & Sons. New York.

[10] EPSTEIN, E. & HAGEN, C. E., 1952 A kinetic study of the absorption of alkali cations by barley roots. Plant Physiol. 27: 457-474.

[11] EPSTEIN, S. & LEGGETT, I. E. 1954 The absorption of alkaline earth cations by barley roots: Kinetics and mechanism. Amer. J. Bot. 41(10): 785-791

[12] FRIED, M. & BROESCHART, 1972 The soil-plant system in relation to inorganic nutrition. Academic Press, London. 358 pp.

[13] FRIED, M. & SHAPIRO, R. E., 1961 Soil-plant relationships in ion uptake. Ann. Rev. Plant Physiol. 12: 91-112.

[14] HOAGLAND, D. R., 1944 Lectures of the inorganic nutrition of plants. In Inorganic Plant Nutrition. Published by Chronica Botânica Comp., Waltham, Massassuchets. 226 pp.

[15] JACKSON, P. C., HENDRICKS, S. B. & VASTA, B. M., 1962 Phosphorylation by barley roots mitochondria and phosphate absorption by barley roots. Plant Physiol. 37: 8-17

[16] LINEWEAVER, H. & BURK, D., 1934 The determination of enzyme dissociation constants. J. Amer. Chem. Soc. 56: 658-666.

[17] LUNDEGARDH, H., 1939 An eletro-chemical theory of salt absorption and respiration. Nature. 143: 203-206.

[18] MAAS, E. V., MOORE,. D. P. & MASON, B. J., 1969 Influence of calcium and magnesium oh manganese absorption. Plant Physiol. 44: 796-800.

[19] MICHAELIS, L. & MENTEN, M. L., 1913 Die Kinetic der Invertinwirkung. Biochem. Z. 49: 333-369.

[20] MITCHELL, P., 1961 Coupling of phosphorylation to elétron and hidrogen transfer by a chemiosmotic type of mechanism. Nature. 191: 144-148.

[21] MITCHELL, P., 1966 Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Biol. Rev. 41: 445-502.

[22] MOORE, D. P., JACOBSON, L. & OVERSTREET, R., 1961 Uptake of calcium by excised barley roots. Plant Physiol. 36: 53-57.

[23] SALISBURY, F. B. & ROSS, C., 1969 Plant physiology. Wadsworth Publishing Comp. Belmont, California. 747 pp.

[24] SMITH, R. C. & EPSTEIN, E., 1964a Ion absorpition by shoot tissue: Technique and first findings with excised leaf tissue of corn. Plant Physiol. 39: (3) : 338-341.

[25] SMITH, R. C. & EPSTEIN, E., 1964b Ion absorption by shoot tissue: Kinetics of potassium and rubidium absorption by corn leaf tissue. Plant Physiol. 39 (6) : 992-996.

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