Urinary catheter: can exposure time and gauge affect biofilm formation?

Odinéa Maria Amorim Batista Rachel Maciel Monteiro Marinila Buzanelo Machado Adriano Menis Ferreira Andreia Rodrigues Moura da Costa Valle Evandro Watanabe Maria Zélia de Araújo Madeiro Maria Eliete Batista Moura About the authors



To assess the effects of exposure time and gauge of Foley catheters in biofilm formation.


In vitro study with samples of Foley catheter fragments made of siliconized latex of different gauges (#14 and #16 French gauge). Artificial urine was produced, which was inoculated with Staphylococcus aureus (ATCC 25923) and Pseudomonas aeruginosa (ATCC 27853) standard bacteria, incubated at 37 °C for 24 hours and 72 hours. The material was analyzed by means of culture (bacterial load) and scanning electron microscopy.


There was no difference in bacterial load of biofilms formed in Foley catheter surfaces with regard to different gauges (p > 0.05). On the other hand, exposure time (24 hours and 72 hours) was a determining factor for P. aeruginosa biofilm formation in Foley catheters (p < 0.05).


Exposure time had an effect on P. aeruginosa biofilm formation in Foley catheters, regardless of gauges.

Biofilm; Urinary catheters; Urinary tract infections; Staphylococcus aureus; Pseudomonas aeruginosa

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