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Urinary catheter: can exposure time and gauge affect biofilm formation?

Abstract

Objective:

To assess the effects of exposure time and gauge of Foley catheters in biofilm formation.

Method:

In vitro study with samples of Foley catheter fragments made of siliconized latex of different gauges (#14 and #16 French gauge). Artificial urine was produced, which was inoculated with Staphylococcus aureus (ATCC 25923) and Pseudomonas aeruginosa (ATCC 27853) standard bacteria, incubated at 37 °C for 24 hours and 72 hours. The material was analyzed by means of culture (bacterial load) and scanning electron microscopy.

Results:

There was no difference in bacterial load of biofilms formed in Foley catheter surfaces with regard to different gauges (p > 0.05). On the other hand, exposure time (24 hours and 72 hours) was a determining factor for P. aeruginosa biofilm formation in Foley catheters (p < 0.05).

Conclusion:

Exposure time had an effect on P. aeruginosa biofilm formation in Foley catheters, regardless of gauges.

Keywords
Biofilm; Urinary catheters; Urinary tract infections; Staphylococcus aureus; Pseudomonas aeruginosa

Escola Paulista de Enfermagem, Universidade Federal de São Paulo R. Napoleão de Barros, 754, 04024-002 São Paulo - SP/Brasil, Tel./Fax: (55 11) 5576 4430 - São Paulo - SP - Brazil
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