We cytogenetically analyzed 18 individuals of Cyphocharax modestus, and 41 individuals of Steindachnerina insculpta from the Tibagi River basin (Paraná State, Brazil). All the specimens had 2n = 54 chromosomes, all meta-submetacentric (M-SM). The presence of 1 or 2 B chromosomes was seen in some individuals of C. modestus of the two sampled populations, showing inter- and intra individual variation. In S. insculpta, B chromosomes were also observed but only in one population, also showing inter- and intra individual variation. C-banding was used to show that the identified B chromosomes were totally heterochromatic in the two examined species.
supernumerary chromosomes; karyotype; C-banding; fish populations